Propionibacterium species are mostly environmental bacteria, some being commensal of mammals including humans, and sometimes pathogenic. These bacteria are poorly identified using routine laboratory methods. Recently,...Propionibacterium species are mostly environmental bacteria, some being commensal of mammals including humans, and sometimes pathogenic. These bacteria are poorly identified using routine laboratory methods. Recently, Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) has emerged as a rapid and efficient method to identify bacterial species. We evaluated the use of MALDI-TOF-MS for identification of all validated Propionibacterium species. Only four of the 15 tested reference strains (26.7%) were correctly identified at the species level, and P. acnes, the most common human pathogenic species was not identified. When applying MALDI-TOF-MS to 48 P. acnes strains, only 18.7% were correctly identified, suggesting an intraspecific variability of proteic profiles among Propionibacterium strains. However, by enriching the Bruker database with spectra from five of these strains and re-testing the other 43 strains against this new database, 93.0% were correctly identified. Our study demonstrates that MALDI-TOF-MS may be used for the identification of Propionibacterium isolates but requires a database enrichment in spectra from additional isolates.展开更多
We investigated the presence of MAP, CMV, or Epstein-Barr virus (EBV) in the saliva of patients presenting with periodontal disease, gingivitis or oral aphthosis. We also measured methylacetate in their breath and loo...We investigated the presence of MAP, CMV, or Epstein-Barr virus (EBV) in the saliva of patients presenting with periodontal disease, gingivitis or oral aphthosis. We also measured methylacetate in their breath and looked for the presence of PA on their tongue. All patients were prospectively enrolled into a two-year cohort study. We compared the group presenting with CD versus the group without CD. 167 patients were enrolled. 24 patients presented with CD. CMV and MAP were found concomitantly in 67% of CD patients (17 patients) whereas only 1 patient (<0.1%;p < 0.001) has these infectious agents in the comparative group. PA was rare in CD (25% versus 70.6%;p < 0.001). MA was low, especially with an empty stomach (0.59 ppm +/? 1.45 versus 5.14 ppm +/? 8.97;p < 0.001). It was concluded that the association MAP + CMV was frequently found in oral lesions of CD at an early stage. Detection of PA, MAP and CMV, as well as breath test could be easily performed and may participate to the early diagnosis of CD.展开更多
The improvement of propionic acid production for antifungal activity, as fermenting by calcium alginate immobilized cells of Propionibacterium acidipropionici TISTR 442 was investigated by using whey as substrate. Opt...The improvement of propionic acid production for antifungal activity, as fermenting by calcium alginate immobilized cells of Propionibacterium acidipropionici TISTR 442 was investigated by using whey as substrate. Optimal condition for immobilization was performed by adjusting tube distance to CaCI2 solution to be 4-6 cm and 7 mL/min flow rate of alginate gel. The production of propionic acid by immobilized cells in a 2 L fermentor using 1% CaCO3 and 5 N KOH to control the pH at 6.5 gave maximum propionic acid and they had consistent potential to recycle 2 rounds of fermentation and produced the total of 29.24 g/L propionic acid （15.85 ± 0.25 g/L and 13.39 ± 0.25 g/L propionic acid from Batch 1 and Batch 2 fermentation, respectively）. Compared to free-cell fermentation, propionic acid productivity increased 20% （0.083 g/h vs. 0.070 g/h） and fermentation time reduced 11% （192 h vs. 216 h） in 2 L fermentor with 40 g/L initial total sugar from whey. The fermented propionic acid as well as the commercial propionic acid from chemical process was able to inhibit the growth of the fungal tested.展开更多
Background:Acne vulgaris is one of the most common skin diseases that can significantly impact a considerable proportion of individuals over their lifetime.Objective:This study focuses on the exploration of the applic...Background:Acne vulgaris is one of the most common skin diseases that can significantly impact a considerable proportion of individuals over their lifetime.Objective:This study focuses on the exploration of the application potential of bamboo vinegar in cosmetics.Materials and Methods:The stock solution of bamboo vinegar is subjected to reduced-pressure distillation at different temperatures to obtain refined bamboo vinegar.Then,inhibition of Propionibacterium acnes(P.acnes)by refined bamboo vinegar is observed.Moreover,the refined bamboo vinegar is adsorbed and released with activated bamboo charcoal as the carrier.In all,this study aims to probe into the mechanism of the controlledrelease system of bamboo vinegar.Results:The results shows that the harmful substances(tar)in bamboo vinegar distilled at 70℃decreased by 94.44%,which is a more notable decrease compared with that in the stock solution.The total organic acid content in bamboo vinegar after reduced-pressure distillation is 11.840%,reaching the national standard for refined bamboo vinegar(GB/T 31734–2015).Additionally,the minimum inhibitory concentration of refined bamboo vinegar against P.acnes using the punch method is 7.90 mg/mL.This indicates that refined bamboo vinegar has the potential as a prospective raw material for formulations in anti-acne cosmetic products.Furthermore,the release rate of bamboo charcoal/bamboo vinegar in water for 15 min reaches 70.57%,which then slows down to a plateau.The slow-release behavior is agreed with the Ritger-Peppas model and is beneficial to relieve the irritation of bamboo vinegar to the skin and lengthen its bacteriostatic duration.Conclusion:The foregoing conclusions can serve as the theoretical foundation for the application of bamboo vinegar in anti-acne cosmetics.展开更多
This study aimed to compare the antimicrobial activities of topical agents against Propionibacterium acnes isolated from patients admitted to a hospital in Shanghai, China. The minimal inhibitory concentrations of the...This study aimed to compare the antimicrobial activities of topical agents against Propionibacterium acnes isolated from patients admitted to a hospital in Shanghai, China. The minimal inhibitory concentrations of the cultured P. acnes were determined in accordance with the Clinical and Laboratory Standards Institute. Susceptibilities to clindamycin and erythromycin were compared in terms of gender, age, disease duration, previous treatment, and disease severity. A total of 69 P. acnes strains were isolated from 98 patients （70.41%）. The susceptibility to triple antibiotic ointment （neomycin/bacitracin/polymyxin B） and bacitracin was 100%. The susceptibility to fusidic acid was 92.7%. The resistance rates to neomycin sulfate, erythromycin, and clindamycin were 11.7%, 49.3%, and 33.4%, respectively. The high resistance rate to clindamycin and erythromycin was significantly affected by gender, previous treatment, and disease severity rather than by age and disease duration. Topical antibiotics should not be used separately for long-term therapy to avoid multiresistance. The use of topical antibiotics should be determined by clinicians on the basis of clinical conditions.展开更多
In this study,15 strains of Lactic acid bacteria(Allata et al.)and 6 strains of propionic acid bacteria(PAB)were firstly tested for their antifungal activity against three spoilage fungi,Aspergillus niger,Penicillium ...In this study,15 strains of Lactic acid bacteria(Allata et al.)and 6 strains of propionic acid bacteria(PAB)were firstly tested for their antifungal activity against three spoilage fungi,Aspergillus niger,Penicillium crustosum and Aspergillus flavus.Two strains of LAB and PAB were selected and assessed,alone or paired,for their abilities to inhibit the most resistant mold,Aspergillus niger.A mixed culture of P.freudenreichii D6 and L.plantarum L9 was found to be the most active and their optimal inoculum was 1×10^(8) and 1×10^(6) CFU/mL,respectively.Furthermore,the in situ antifungal effect of the mixed culture was evaluated against bakery product spoilage fungi.It was found that the growth of airborne fungi was delayed for up to 7 days.The cell number of L.plantarum L9 was slightly increased by the presence of P.freudenreichii D6.Quantification of three main acids(propionic,lactic and acetic acid)produced by the protective cultures suggested that acetic acid was mainly responsible for the antifungal activity.In conclusion,the mixed culture of P.freudenreichii and L.plantarum may be exploited as biopreservatives in bakery products.展开更多
Objective Little information is available to guide treatment strategies regarding patients with unexpected positive cultures （UPC）,including Propionibacterium acnes （PA）,without overt signs of infection in revisio...Objective Little information is available to guide treatment strategies regarding patients with unexpected positive cultures （UPC）,including Propionibacterium acnes （PA）,without overt signs of infection in revision shoulder arthroplasty （RSA）.The purpose of our study was to analyze the prevalence,clinical meaning,treatment and prognosis of UPC in RSA.Data sources and study selection We performed a systematic review of the literature between 1950 and 2013 for all studies reporting on UPC for PA.Studies with the prevalence and prognosis of patients with UPC in RSA were analyzed.Results Six studies meeting our inclusion criteria yielded data for 1 402 patients who underwent a total of 1405 RSA.Based on the available data,following RSA 235 shoulders had UPCs with a pooled percentage of 16.7％ （235/1405）.The most commonly isolated bacteria from shoulders following RSA with UPCs was PA with pooled percentages of 63.4％ （149/235）.Occurrence of true infection from UPCs after RSA was seen in 24 shoulders （24/235,10.2％）.Antibiotic use did not influence the rate of the occurrence of true infection from UPCs （P=0.498）.Conclusions Our study showed a low risk of having a true infection from UPCs after RSA without clinical signs of preoperative infection at the time of the surgery.Therefore,prolonged antibiotic therapy may not be necessary in these patients.展开更多
To verify the inhibitory ability of the combination on the biofilm production of Propionibacterium acnes and its ameliorative effect on facial acne problems.The ability of the combination to inhibit the biofilm format...To verify the inhibitory ability of the combination on the biofilm production of Propionibacterium acnes and its ameliorative effect on facial acne problems.The ability of the combination to inhibit the biofilm formation of Propionibacterium acnes was verified by suspension quantification and crystal violet staining,and then 60 effective volunteers were selected for the efficacy measurement of topical acne elimination combinations+bases for 4 consecutive weeks,which were measured by a professional instrument,and the volunteers’facial acne lesion areas and acne lesion colours were evaluated respectively.In vitro tests verified that the combination can inhibit the formation of P.acnes biofilm and will not completely kill P.acnes and will not disrupt the micro-ecological balance of the skin;3%and 5%acne combinations can reduce the biofilm of P.acnes by 61.3%and 61.6%respectively;after 4 weeks of continuous use,the area of acne lesions of the samples with 5%acne combinations decreased by 54.61%and the colour of acne lesions decreased by 54.61%compared with the area of acne lesions before the use of the combination.54.61%,acne lesion colour a^(*)decreased by 13.74%,and the subjects’self-assessment of the mildness and non-irritation of the samples in the test group had a 100%agreement rate.The acne combination can improve acne problems by inhibiting the biofilm formation of Propionibacterium acnes,and has a mild and effective acne elimination effect.展开更多
[Objectives]This study was conducted to investigate the antibacterial effect of a self-designed acne-relieving Chinese herbal formula and prepare a kind of acne-removing soap according to the antibacterial effect.[Met...[Objectives]This study was conducted to investigate the antibacterial effect of a self-designed acne-relieving Chinese herbal formula and prepare a kind of acne-removing soap according to the antibacterial effect.[Methods]Chinese herbs Lonicera japonica,Taraxacum mongolicum,Scutellaria baicalensis,Angelica dahurica,Centella asiatica and Aloe vera,were decocted with water,and the antibacterial effects on Staphylococcus aureus,Staphylococcus albus,Candida albicans and Propionibacterium acnei were tested by inhibition zone experiments.The soap base was prepared by saponification of coconut oil,palm oil,hazelnut oil and sodium hydroxide,and then the herbal liquid was added into the soap.[Results]The liquid had bacteriostatic effect on S.aureus,S.albus and P.acnes,but no inhibitory effect on C.albicans.The best bacteriostatic effect on S.aureus and S.albus was achieved at 330 mg/ml,and the best bacteriostatic effect on P.acnes was at 160 mg/ml.[Conclusions]The Chinese herbal formula has antibacterial effect.The handmade soap made from the Chinese herbal formula has the characteristics of smooth surface,moderate hardness,fine texture,rich foam and fresh smell.展开更多
文摘Propionibacterium species are mostly environmental bacteria, some being commensal of mammals including humans, and sometimes pathogenic. These bacteria are poorly identified using routine laboratory methods. Recently, Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) has emerged as a rapid and efficient method to identify bacterial species. We evaluated the use of MALDI-TOF-MS for identification of all validated Propionibacterium species. Only four of the 15 tested reference strains (26.7%) were correctly identified at the species level, and P. acnes, the most common human pathogenic species was not identified. When applying MALDI-TOF-MS to 48 P. acnes strains, only 18.7% were correctly identified, suggesting an intraspecific variability of proteic profiles among Propionibacterium strains. However, by enriching the Bruker database with spectra from five of these strains and re-testing the other 43 strains against this new database, 93.0% were correctly identified. Our study demonstrates that MALDI-TOF-MS may be used for the identification of Propionibacterium isolates but requires a database enrichment in spectra from additional isolates.
文摘We investigated the presence of MAP, CMV, or Epstein-Barr virus (EBV) in the saliva of patients presenting with periodontal disease, gingivitis or oral aphthosis. We also measured methylacetate in their breath and looked for the presence of PA on their tongue. All patients were prospectively enrolled into a two-year cohort study. We compared the group presenting with CD versus the group without CD. 167 patients were enrolled. 24 patients presented with CD. CMV and MAP were found concomitantly in 67% of CD patients (17 patients) whereas only 1 patient (<0.1%;p < 0.001) has these infectious agents in the comparative group. PA was rare in CD (25% versus 70.6%;p < 0.001). MA was low, especially with an empty stomach (0.59 ppm +/? 1.45 versus 5.14 ppm +/? 8.97;p < 0.001). It was concluded that the association MAP + CMV was frequently found in oral lesions of CD at an early stage. Detection of PA, MAP and CMV, as well as breath test could be easily performed and may participate to the early diagnosis of CD.
文摘The improvement of propionic acid production for antifungal activity, as fermenting by calcium alginate immobilized cells of Propionibacterium acidipropionici TISTR 442 was investigated by using whey as substrate. Optimal condition for immobilization was performed by adjusting tube distance to CaCI2 solution to be 4-6 cm and 7 mL/min flow rate of alginate gel. The production of propionic acid by immobilized cells in a 2 L fermentor using 1% CaCO3 and 5 N KOH to control the pH at 6.5 gave maximum propionic acid and they had consistent potential to recycle 2 rounds of fermentation and produced the total of 29.24 g/L propionic acid （15.85 ± 0.25 g/L and 13.39 ± 0.25 g/L propionic acid from Batch 1 and Batch 2 fermentation, respectively）. Compared to free-cell fermentation, propionic acid productivity increased 20% （0.083 g/h vs. 0.070 g/h） and fermentation time reduced 11% （192 h vs. 216 h） in 2 L fermentor with 40 g/L initial total sugar from whey. The fermented propionic acid as well as the commercial propionic acid from chemical process was able to inhibit the growth of the fungal tested.
文摘Background:Acne vulgaris is one of the most common skin diseases that can significantly impact a considerable proportion of individuals over their lifetime.Objective:This study focuses on the exploration of the application potential of bamboo vinegar in cosmetics.Materials and Methods:The stock solution of bamboo vinegar is subjected to reduced-pressure distillation at different temperatures to obtain refined bamboo vinegar.Then,inhibition of Propionibacterium acnes(P.acnes)by refined bamboo vinegar is observed.Moreover,the refined bamboo vinegar is adsorbed and released with activated bamboo charcoal as the carrier.In all,this study aims to probe into the mechanism of the controlledrelease system of bamboo vinegar.Results:The results shows that the harmful substances(tar)in bamboo vinegar distilled at 70℃decreased by 94.44%,which is a more notable decrease compared with that in the stock solution.The total organic acid content in bamboo vinegar after reduced-pressure distillation is 11.840%,reaching the national standard for refined bamboo vinegar(GB/T 31734–2015).Additionally,the minimum inhibitory concentration of refined bamboo vinegar against P.acnes using the punch method is 7.90 mg/mL.This indicates that refined bamboo vinegar has the potential as a prospective raw material for formulations in anti-acne cosmetic products.Furthermore,the release rate of bamboo charcoal/bamboo vinegar in water for 15 min reaches 70.57%,which then slows down to a plateau.The slow-release behavior is agreed with the Ritger-Peppas model and is beneficial to relieve the irritation of bamboo vinegar to the skin and lengthen its bacteriostatic duration.Conclusion:The foregoing conclusions can serve as the theoretical foundation for the application of bamboo vinegar in anti-acne cosmetics.
基金The paper was supervised by Dr. Kefei Kang. This work was supported by the Scientific Research Project of Huashan Hospital, Fudan University （No. 2013QD07） and the National Natural Science Foundation of China （No. 81402615）.
文摘This study aimed to compare the antimicrobial activities of topical agents against Propionibacterium acnes isolated from patients admitted to a hospital in Shanghai, China. The minimal inhibitory concentrations of the cultured P. acnes were determined in accordance with the Clinical and Laboratory Standards Institute. Susceptibilities to clindamycin and erythromycin were compared in terms of gender, age, disease duration, previous treatment, and disease severity. A total of 69 P. acnes strains were isolated from 98 patients （70.41%）. The susceptibility to triple antibiotic ointment （neomycin/bacitracin/polymyxin B） and bacitracin was 100%. The susceptibility to fusidic acid was 92.7%. The resistance rates to neomycin sulfate, erythromycin, and clindamycin were 11.7%, 49.3%, and 33.4%, respectively. The high resistance rate to clindamycin and erythromycin was significantly affected by gender, previous treatment, and disease severity rather than by age and disease duration. Topical antibiotics should not be used separately for long-term therapy to avoid multiresistance. The use of topical antibiotics should be determined by clinicians on the basis of clinical conditions.
文摘In this study,15 strains of Lactic acid bacteria(Allata et al.)and 6 strains of propionic acid bacteria(PAB)were firstly tested for their antifungal activity against three spoilage fungi,Aspergillus niger,Penicillium crustosum and Aspergillus flavus.Two strains of LAB and PAB were selected and assessed,alone or paired,for their abilities to inhibit the most resistant mold,Aspergillus niger.A mixed culture of P.freudenreichii D6 and L.plantarum L9 was found to be the most active and their optimal inoculum was 1×10^(8) and 1×10^(6) CFU/mL,respectively.Furthermore,the in situ antifungal effect of the mixed culture was evaluated against bakery product spoilage fungi.It was found that the growth of airborne fungi was delayed for up to 7 days.The cell number of L.plantarum L9 was slightly increased by the presence of P.freudenreichii D6.Quantification of three main acids(propionic,lactic and acetic acid)produced by the protective cultures suggested that acetic acid was mainly responsible for the antifungal activity.In conclusion,the mixed culture of P.freudenreichii and L.plantarum may be exploited as biopreservatives in bakery products.
文摘Objective Little information is available to guide treatment strategies regarding patients with unexpected positive cultures （UPC）,including Propionibacterium acnes （PA）,without overt signs of infection in revision shoulder arthroplasty （RSA）.The purpose of our study was to analyze the prevalence,clinical meaning,treatment and prognosis of UPC in RSA.Data sources and study selection We performed a systematic review of the literature between 1950 and 2013 for all studies reporting on UPC for PA.Studies with the prevalence and prognosis of patients with UPC in RSA were analyzed.Results Six studies meeting our inclusion criteria yielded data for 1 402 patients who underwent a total of 1405 RSA.Based on the available data,following RSA 235 shoulders had UPCs with a pooled percentage of 16.7％ （235/1405）.The most commonly isolated bacteria from shoulders following RSA with UPCs was PA with pooled percentages of 63.4％ （149/235）.Occurrence of true infection from UPCs after RSA was seen in 24 shoulders （24/235,10.2％）.Antibiotic use did not influence the rate of the occurrence of true infection from UPCs （P=0.498）.Conclusions Our study showed a low risk of having a true infection from UPCs after RSA without clinical signs of preoperative infection at the time of the surgery.Therefore,prolonged antibiotic therapy may not be necessary in these patients.
文摘To verify the inhibitory ability of the combination on the biofilm production of Propionibacterium acnes and its ameliorative effect on facial acne problems.The ability of the combination to inhibit the biofilm formation of Propionibacterium acnes was verified by suspension quantification and crystal violet staining,and then 60 effective volunteers were selected for the efficacy measurement of topical acne elimination combinations+bases for 4 consecutive weeks,which were measured by a professional instrument,and the volunteers’facial acne lesion areas and acne lesion colours were evaluated respectively.In vitro tests verified that the combination can inhibit the formation of P.acnes biofilm and will not completely kill P.acnes and will not disrupt the micro-ecological balance of the skin;3%and 5%acne combinations can reduce the biofilm of P.acnes by 61.3%and 61.6%respectively;after 4 weeks of continuous use,the area of acne lesions of the samples with 5%acne combinations decreased by 54.61%and the colour of acne lesions decreased by 54.61%compared with the area of acne lesions before the use of the combination.54.61%,acne lesion colour a^(*)decreased by 13.74%,and the subjects’self-assessment of the mildness and non-irritation of the samples in the test group had a 100%agreement rate.The acne combination can improve acne problems by inhibiting the biofilm formation of Propionibacterium acnes,and has a mild and effective acne elimination effect.
基金Supported by National Undergraduate Innovation and Entrepreneurship Training Program of Guangxi University of Chinese Medicine in 2022(202210600026).
文摘[Objectives]This study was conducted to investigate the antibacterial effect of a self-designed acne-relieving Chinese herbal formula and prepare a kind of acne-removing soap according to the antibacterial effect.[Methods]Chinese herbs Lonicera japonica,Taraxacum mongolicum,Scutellaria baicalensis,Angelica dahurica,Centella asiatica and Aloe vera,were decocted with water,and the antibacterial effects on Staphylococcus aureus,Staphylococcus albus,Candida albicans and Propionibacterium acnei were tested by inhibition zone experiments.The soap base was prepared by saponification of coconut oil,palm oil,hazelnut oil and sodium hydroxide,and then the herbal liquid was added into the soap.[Results]The liquid had bacteriostatic effect on S.aureus,S.albus and P.acnes,but no inhibitory effect on C.albicans.The best bacteriostatic effect on S.aureus and S.albus was achieved at 330 mg/ml,and the best bacteriostatic effect on P.acnes was at 160 mg/ml.[Conclusions]The Chinese herbal formula has antibacterial effect.The handmade soap made from the Chinese herbal formula has the characteristics of smooth surface,moderate hardness,fine texture,rich foam and fresh smell.
