Propamocarb is an agricultural chemical that has been widely used to protect cucumber plants from downy mildew. To understand the mechanisms of cucumber defense responses to propamocarb, we investigated the physiologi...Propamocarb is an agricultural chemical that has been widely used to protect cucumber plants from downy mildew. To understand the mechanisms of cucumber defense responses to propamocarb, we investigated the physiological and proteomic responses of the cucumber line D0351 with propamocarb application. We found that after treatment with propamocarb, the activities of detoxifying enzymes （glutathione reductase, GR; glutathione S-tramsferase, GST） and soluble sugar content of cucumber fruit were signiifcantly increased, but malonaldehyde （MDA） content was signiifcantly reduced. To identify components of propamocarb responsive signaling, we compared the high resolution two-dimensional gel electrophoresis （2-DE） protein proifles of control and propamocarb-treated fruits, and identiifed 18 differentially expressed （13 up-regulated and 5 down-regulated） proteins induced by propamocarb which were determined by matrix-assisted laser desorption/ionization time-of-lfight mass spectrometry （MALDI-TOF-MS）. The majority of the proteins had functions related to detoxication, energy and transport, protein biosynthesis, regulating reactions and defending against stresses. A real-time quantitative reverse transcriptional-polymerase chain reaction （qRT-PCR） was used to compare transcript and protein accumulation patterns for 18 candidate proteins, and the expression of 14 was consistent at both transcript and protein levels. The responses of cucumber proteome to propamocarb seemed complex; the identified proteins may play an important role in regulating adaptation activities following exposure to propamocarb. Data presented herein may shed light on understanding cucumber fruit defense responses under propamocarb treatment.展开更多
Propamocarb （PM） residue in cucumber （Cucumis sativus L.） receives little attention. As is well known to all, high PM residue of cucumber could lead to increase in the violation rates of maximum residue limits and...Propamocarb （PM） residue in cucumber （Cucumis sativus L.） receives little attention. As is well known to all, high PM residue of cucumber could lead to increase in the violation rates of maximum residue limits and ultimately cause harm to human health. Knowledge of PM residue could help cucumber breeders in developing cultivars with low PM residue and improving cucumber quality. In this study, 32 representative cucumber accessions （26 breeding lines and six cultivars） from different regions of China were evaluated for their PM residue in fruit and leaf to provide meaning to the subjective rating, which was highly correlated with PM residual content of fruit （r=0.97） and leaf （r=0.94）. In addition, PM residual content of North China ecotype was the highest and Pickling ecotype was the lowest in fruit and leaf of cucumber. The leaf had significantly higher （P〈0.01） PM residual content than the fruit, and poor correlation between leaf and fruit was represented. This study verified PM residual relationship between fruit and leaf, and laid the foundation for further identification of germplasm resources and breeding of new varieties for low PM residue of cucumber.展开更多
An easy, simple and efficient analytical method was standardized and validated for the estimation of residues of propamocarb in tomato and soil. QuEChERS method included extraction of the sample with ethyl acetate and...An easy, simple and efficient analytical method was standardized and validated for the estimation of residues of propamocarb in tomato and soil. QuEChERS method included extraction of the sample with ethyl acetate and cleanup by treatment with PSA and graphatised carbon. Final clear extracts of ethyl acetate were concentrated under vacuum to almost dryness and reconstituted into hexane. The residues of propamocarbwere estimated using gas chromatograph-mass spectrometry (GC-MS). Propamocarb presented a distinct peak at retention time of 8.962 min. Consistent recoveries of propamocarb ranging from 87 to 92 percent were observed when samples were spiked at 0.10, 0.50 and 1.00 mg·kg–1 levels. The limit of quantification (LOQ) of this method was determined to be 0.10 mg·kg–1.展开更多
[目的]筛选适用于大葱枯萎病防治的复配药剂。[方法]室内采用菌丝生长速率法测定霜霉威盐酸盐和霉灵及其不同复配组合对大葱枯萎病菌的生物活性,田间采用沟施法测定复配药剂的田间防效。[结果]室内生物活性测定结果表明:霜霉威盐酸盐和...[目的]筛选适用于大葱枯萎病防治的复配药剂。[方法]室内采用菌丝生长速率法测定霜霉威盐酸盐和霉灵及其不同复配组合对大葱枯萎病菌的生物活性,田间采用沟施法测定复配药剂的田间防效。[结果]室内生物活性测定结果表明:霜霉威盐酸盐和霉灵配比为3∶1时,对大葱枯萎病菌的增效作用最显著。根据最佳质量比配制的药剂2%霜霉·霉灵颗粒剂施药量为1200、1500 g a.i./hm^(2),对大葱枯萎病均有较好的防治效果,2022年防效为70.65%和72.87%,2023年防效为72.05%和73.49%。[结论]2%霜霉·霉灵颗粒剂可用于田间防治大葱枯萎病。展开更多
[目的]建立一种同时测定687.5 g/L氟菌·霜霉威悬浮剂中2种有效成分的高效液相色谱检测方法。[方法]采用带有二极管阵列检测器的反相高效液相色谱,使用Eclipse Plus C18柱,以乙腈和氨水作为流动相,在特定波长210 nm下对试样中的霜...[目的]建立一种同时测定687.5 g/L氟菌·霜霉威悬浮剂中2种有效成分的高效液相色谱检测方法。[方法]采用带有二极管阵列检测器的反相高效液相色谱,使用Eclipse Plus C18柱,以乙腈和氨水作为流动相,在特定波长210 nm下对试样中的霜霉威盐酸盐和氟吡菌胺进行分离检测,外标法定量。[结果]霜霉威盐酸盐和氟吡菌胺的线性相关系数均为0.999 97,变异系数分别为0.39%和0.48%,平均回收率分别为100.62%和99.10%。[结论]该方法操作简便、准确性高,适用于氟菌·霜霉威悬浮剂样品的定量分析。展开更多
ABC转运蛋白基因与植物的多抗耐药性密切相关,是植物抗农药机理研究的热点。克隆黄瓜ABC转运蛋白基因abca19,分析其编码蛋白的氨基酸序列、理化性质和结构,了解其在霜霉威胁迫条件下的表达规律,为abca19的研究奠定基础,为黄瓜抗农药霜...ABC转运蛋白基因与植物的多抗耐药性密切相关,是植物抗农药机理研究的热点。克隆黄瓜ABC转运蛋白基因abca19,分析其编码蛋白的氨基酸序列、理化性质和结构,了解其在霜霉威胁迫条件下的表达规律,为abca19的研究奠定基础,为黄瓜抗农药霜霉威机理研究积累材料。以D0351为材料,根据黄瓜基因组数据库中Csa7M368750.1基因编码区全序列,应用Primer Premier 5.0软件设计引物,从黄瓜果实cDNA中克隆abca19基因的开放阅读框。定量PCR分析霜霉威胁迫处理条件下,黄瓜果实不同处理时间和黄瓜不同部位abca19的表达变化。abca19序列长度为921bp,注册到Gene Bank,登录号为KC123181。abca19编码306个氨基酸,与大豆（Glycine max）等植物abca19的同源性为80%。该蛋白是一个疏水蛋白,无跨膜结构,包含6个α-螺旋结构,无信号肽。具有蛋白激酶C磷酸化位点、N-豆蔻酰化位点、N-糖基化位点和酪蛋白激酶Ⅱ磷酸化位点等多个活性位点。霜霉威处理条件下,0.5-6h黄瓜果实处理组abca19相对表达量极显著高于对照,9-48h对照组abca19相对表达量高于处理组,但二者间差异不显著。不同处理时间点（3、9和24h）,叶片中abca19相对表达量最高,果实中其次,茎中最低。成功克隆到黄瓜abca19,该基因具有已知物种abca19的特征。霜霉威处理条件下,黄瓜果实abca19参与农药霜霉威胁迫响应,反应迅速且具有时限性。在黄瓜叶片和果实中,abca19积极参与对农药霜霉威胁迫的响应,茎中响应较弱。展开更多
基金supported by the National Natural Science Foundation of China(21372158)
文摘Propamocarb is an agricultural chemical that has been widely used to protect cucumber plants from downy mildew. To understand the mechanisms of cucumber defense responses to propamocarb, we investigated the physiological and proteomic responses of the cucumber line D0351 with propamocarb application. We found that after treatment with propamocarb, the activities of detoxifying enzymes （glutathione reductase, GR; glutathione S-tramsferase, GST） and soluble sugar content of cucumber fruit were signiifcantly increased, but malonaldehyde （MDA） content was signiifcantly reduced. To identify components of propamocarb responsive signaling, we compared the high resolution two-dimensional gel electrophoresis （2-DE） protein proifles of control and propamocarb-treated fruits, and identiifed 18 differentially expressed （13 up-regulated and 5 down-regulated） proteins induced by propamocarb which were determined by matrix-assisted laser desorption/ionization time-of-lfight mass spectrometry （MALDI-TOF-MS）. The majority of the proteins had functions related to detoxication, energy and transport, protein biosynthesis, regulating reactions and defending against stresses. A real-time quantitative reverse transcriptional-polymerase chain reaction （qRT-PCR） was used to compare transcript and protein accumulation patterns for 18 candidate proteins, and the expression of 14 was consistent at both transcript and protein levels. The responses of cucumber proteome to propamocarb seemed complex; the identified proteins may play an important role in regulating adaptation activities following exposure to propamocarb. Data presented herein may shed light on understanding cucumber fruit defense responses under propamocarb treatment.
基金Supported by the National Natural Science Foundation of China(31272158)863 Program of the National Science and Technology of China(2012AA100105)
文摘Propamocarb （PM） residue in cucumber （Cucumis sativus L.） receives little attention. As is well known to all, high PM residue of cucumber could lead to increase in the violation rates of maximum residue limits and ultimately cause harm to human health. Knowledge of PM residue could help cucumber breeders in developing cultivars with low PM residue and improving cucumber quality. In this study, 32 representative cucumber accessions （26 breeding lines and six cultivars） from different regions of China were evaluated for their PM residue in fruit and leaf to provide meaning to the subjective rating, which was highly correlated with PM residual content of fruit （r=0.97） and leaf （r=0.94）. In addition, PM residual content of North China ecotype was the highest and Pickling ecotype was the lowest in fruit and leaf of cucumber. The leaf had significantly higher （P〈0.01） PM residual content than the fruit, and poor correlation between leaf and fruit was represented. This study verified PM residual relationship between fruit and leaf, and laid the foundation for further identification of germplasm resources and breeding of new varieties for low PM residue of cucumber.
文摘An easy, simple and efficient analytical method was standardized and validated for the estimation of residues of propamocarb in tomato and soil. QuEChERS method included extraction of the sample with ethyl acetate and cleanup by treatment with PSA and graphatised carbon. Final clear extracts of ethyl acetate were concentrated under vacuum to almost dryness and reconstituted into hexane. The residues of propamocarbwere estimated using gas chromatograph-mass spectrometry (GC-MS). Propamocarb presented a distinct peak at retention time of 8.962 min. Consistent recoveries of propamocarb ranging from 87 to 92 percent were observed when samples were spiked at 0.10, 0.50 and 1.00 mg·kg–1 levels. The limit of quantification (LOQ) of this method was determined to be 0.10 mg·kg–1.
文摘[目的]筛选适用于大葱枯萎病防治的复配药剂。[方法]室内采用菌丝生长速率法测定霜霉威盐酸盐和霉灵及其不同复配组合对大葱枯萎病菌的生物活性,田间采用沟施法测定复配药剂的田间防效。[结果]室内生物活性测定结果表明:霜霉威盐酸盐和霉灵配比为3∶1时,对大葱枯萎病菌的增效作用最显著。根据最佳质量比配制的药剂2%霜霉·霉灵颗粒剂施药量为1200、1500 g a.i./hm^(2),对大葱枯萎病均有较好的防治效果,2022年防效为70.65%和72.87%,2023年防效为72.05%和73.49%。[结论]2%霜霉·霉灵颗粒剂可用于田间防治大葱枯萎病。
文摘ABC转运蛋白基因与植物的多抗耐药性密切相关,是植物抗农药机理研究的热点。克隆黄瓜ABC转运蛋白基因abca19,分析其编码蛋白的氨基酸序列、理化性质和结构,了解其在霜霉威胁迫条件下的表达规律,为abca19的研究奠定基础,为黄瓜抗农药霜霉威机理研究积累材料。以D0351为材料,根据黄瓜基因组数据库中Csa7M368750.1基因编码区全序列,应用Primer Premier 5.0软件设计引物,从黄瓜果实cDNA中克隆abca19基因的开放阅读框。定量PCR分析霜霉威胁迫处理条件下,黄瓜果实不同处理时间和黄瓜不同部位abca19的表达变化。abca19序列长度为921bp,注册到Gene Bank,登录号为KC123181。abca19编码306个氨基酸,与大豆（Glycine max）等植物abca19的同源性为80%。该蛋白是一个疏水蛋白,无跨膜结构,包含6个α-螺旋结构,无信号肽。具有蛋白激酶C磷酸化位点、N-豆蔻酰化位点、N-糖基化位点和酪蛋白激酶Ⅱ磷酸化位点等多个活性位点。霜霉威处理条件下,0.5-6h黄瓜果实处理组abca19相对表达量极显著高于对照,9-48h对照组abca19相对表达量高于处理组,但二者间差异不显著。不同处理时间点（3、9和24h）,叶片中abca19相对表达量最高,果实中其次,茎中最低。成功克隆到黄瓜abca19,该基因具有已知物种abca19的特征。霜霉威处理条件下,黄瓜果实abca19参与农药霜霉威胁迫响应,反应迅速且具有时限性。在黄瓜叶片和果实中,abca19积极参与对农药霜霉威胁迫的响应,茎中响应较弱。
