目的:寻找体内能够与Fbxl16相互作用的蛋白质,用以揭示该蛋白的功能及其在脊髓损伤修复过程中的作用.方法:通过RT-PCR技术克隆scirr1基因编码区序列,将其克隆入pSos载体以构建质粒pSos-scirr1.把该重组质粒表达的融合蛋白hSos-Fbxl16作...目的:寻找体内能够与Fbxl16相互作用的蛋白质,用以揭示该蛋白的功能及其在脊髓损伤修复过程中的作用.方法:通过RT-PCR技术克隆scirr1基因编码区序列,将其克隆入pSos载体以构建质粒pSos-scirr1.把该重组质粒表达的融合蛋白hSos-Fbxl16作为诱饵蛋白,用改良的酵母双杂交技术筛选大鼠脑cDNA文库中能与Fbxl16相互作用的蛋白质,最后用酵母双杂交回转实验对获得的相互作用的可靠性加以验证.结果:成功构建了融合蛋白表达质粒pSos-scirr1之后,筛选出了14个与Fbxl16阳性相互作用的蛋白质;通过回转实验验证,确定其中两个蛋白能与Fbxl16相互作用,分别是cAMP依赖蛋白激酶α型催化亚基(protein kinase,cAMP-de-pendent,catalytic,alpha,Prkaca)和衣被蛋白复合物ζ亚基(coatomer protein complex,subunit zeta1,Copz1).结论:Fbxl16和Prkaca的相互作用为解释cAMP通路与脊髓损伤的关系提供了新思路,提示Fbxl16与细胞内物质转运有关.展开更多
Fibrolamellar hepatocellular carcinoma(FLC)is a rare but aggressive liver cancer of children that occurs predominantly in teenagers without a history of liver disease.Surgical resection remains the only therapeutic op...Fibrolamellar hepatocellular carcinoma(FLC)is a rare but aggressive liver cancer of children that occurs predominantly in teenagers without a history of liver disease.Surgical resection remains the only therapeutic option,and the recurrence rate is extremely high(>50%within 3 years).A newly discovered chromosomal deletion that occurs in the majority of FLCs generates a novel kinase fusion between DnaJ heat shock protein family member B1(DNAJB1)and protein kinase cAMP-activated catalytic subunit alpha(PRKACA)(DNAJB1-PRKACA).Despite its high penetrance and apparent specificity for FLC,the oncogenic role of this fusion event remains unclear.In this review article,we discuss the histology,presentation and diagnosis,current treatment,and roles of the DNAJB1-PRKACA as well as research models contributing to our understanding of this disease.展开更多
文摘目的:寻找体内能够与Fbxl16相互作用的蛋白质,用以揭示该蛋白的功能及其在脊髓损伤修复过程中的作用.方法:通过RT-PCR技术克隆scirr1基因编码区序列,将其克隆入pSos载体以构建质粒pSos-scirr1.把该重组质粒表达的融合蛋白hSos-Fbxl16作为诱饵蛋白,用改良的酵母双杂交技术筛选大鼠脑cDNA文库中能与Fbxl16相互作用的蛋白质,最后用酵母双杂交回转实验对获得的相互作用的可靠性加以验证.结果:成功构建了融合蛋白表达质粒pSos-scirr1之后,筛选出了14个与Fbxl16阳性相互作用的蛋白质;通过回转实验验证,确定其中两个蛋白能与Fbxl16相互作用,分别是cAMP依赖蛋白激酶α型催化亚基(protein kinase,cAMP-de-pendent,catalytic,alpha,Prkaca)和衣被蛋白复合物ζ亚基(coatomer protein complex,subunit zeta1,Copz1).结论:Fbxl16和Prkaca的相互作用为解释cAMP通路与脊髓损伤的关系提供了新思路,提示Fbxl16与细胞内物质转运有关.
文摘Fibrolamellar hepatocellular carcinoma(FLC)is a rare but aggressive liver cancer of children that occurs predominantly in teenagers without a history of liver disease.Surgical resection remains the only therapeutic option,and the recurrence rate is extremely high(>50%within 3 years).A newly discovered chromosomal deletion that occurs in the majority of FLCs generates a novel kinase fusion between DnaJ heat shock protein family member B1(DNAJB1)and protein kinase cAMP-activated catalytic subunit alpha(PRKACA)(DNAJB1-PRKACA).Despite its high penetrance and apparent specificity for FLC,the oncogenic role of this fusion event remains unclear.In this review article,we discuss the histology,presentation and diagnosis,current treatment,and roles of the DNAJB1-PRKACA as well as research models contributing to our understanding of this disease.
