Aeromonas veronii is considered an emerging food-borne pathogen associated with a significant threat to public health,distributed in various aquatic environments and products.Hanks-type serine/threonine kinases(STKs)p...Aeromonas veronii is considered an emerging food-borne pathogen associated with a significant threat to public health,distributed in various aquatic environments and products.Hanks-type serine/threonine kinases(STKs)play a critical role in the pathogenesis of pathogens.However,the function of A.veronii STKs is currently unclear.By constructing a markerless prk A in-frame deletion strain,Δprk A,we found that i)the colonies of theΔprk A strain were larger after 1 h of high temperature at 50℃compared with the wild-type strain TH0426 and the complementary strain C-prk A,and the number of viable bacteria of theΔprk A strain increased significantly;ii)theΔprk A strain significantly enhanced the adhesion ability to epithelioma papulosum cyprini(EPC)cells;iii)theΔprk A strain was significantly more virulent than the TH0426 strain,at both the cellular and animal levels;and iv)RNA-seq results showed a total of 984 differentially expressed genes(DEGs)between theΔprk A strain and the TH0426 strain,which were enriched in 70 Kyoto Encyclopedia of Genes and Genomes(KEGG)metabolic pathways,mainly involved in bacterial ribosomes,flagellar assembly,type Ⅱ secretion system(T2SS),and lipopolysaccharide metabolic pathways.Taken together,the findings of this study indicate that the Hanks-type STK Prk A negatively regulates several biological processes,such as the temperature tolerance and virulence of A.veronii.The results of this study provide an important reference for further elucidation of the pathogenesis of A.veronii.展开更多
基金The Shandong Natural Science Foundation Youth Fund Project(ZR2022QC079,ZR2023QD024)provided funding for this work。
文摘Aeromonas veronii is considered an emerging food-borne pathogen associated with a significant threat to public health,distributed in various aquatic environments and products.Hanks-type serine/threonine kinases(STKs)play a critical role in the pathogenesis of pathogens.However,the function of A.veronii STKs is currently unclear.By constructing a markerless prk A in-frame deletion strain,Δprk A,we found that i)the colonies of theΔprk A strain were larger after 1 h of high temperature at 50℃compared with the wild-type strain TH0426 and the complementary strain C-prk A,and the number of viable bacteria of theΔprk A strain increased significantly;ii)theΔprk A strain significantly enhanced the adhesion ability to epithelioma papulosum cyprini(EPC)cells;iii)theΔprk A strain was significantly more virulent than the TH0426 strain,at both the cellular and animal levels;and iv)RNA-seq results showed a total of 984 differentially expressed genes(DEGs)between theΔprk A strain and the TH0426 strain,which were enriched in 70 Kyoto Encyclopedia of Genes and Genomes(KEGG)metabolic pathways,mainly involved in bacterial ribosomes,flagellar assembly,type Ⅱ secretion system(T2SS),and lipopolysaccharide metabolic pathways.Taken together,the findings of this study indicate that the Hanks-type STK Prk A negatively regulates several biological processes,such as the temperature tolerance and virulence of A.veronii.The results of this study provide an important reference for further elucidation of the pathogenesis of A.veronii.
