Peripheral nerve regeneration using a novel nerve conduit （PRGD/PDLLA/β TCP/NGF） was evaluated, which was made of RGD peptide modified poly{（lactic acid）-co-[（glycolic acid）-alt-（L-lysine）]} （PRGD）, poly（d...Peripheral nerve regeneration using a novel nerve conduit （PRGD/PDLLA/β TCP/NGF） was evaluated, which was made of RGD peptide modified poly{（lactic acid）-co-[（glycolic acid）-alt-（L-lysine）]} （PRGD）, poly（d,l-lactic acid） （PDLLA） and β-tricalcium phosphate （β-TCP）. And the effectiveness was compared with that of PRGD/PDLLA/β-TCP, PDLLA and autograft in terms of nerve regeneration across a gap. Both of biodegradablity and cell-biocompatibility of the novel nerve conduit were evaluated in vitro. The results show that PRGD/PDLLA/β-TCP/NGF composite ma terials have better biodegradation properties and cell affinity than PDLLA, and could promote the RSC96 Schwann cells adhesion, proliferation and growth on the surface of materials. PRGD/PDLLA/ β-TCP/NGF composite conduit was significantly superior to that of the PDLLA conduit in histological and axon morphologic index. PRGD/PDLLA/β-TCP/NGF conduit is more beneficial to nerve regeneration than PDLLA conduit. The biodegradable PDLLA/PRGD/β-TCP/NGF conduit has a good biocompatibility with rats tissue and it could effectively promote the nerve regeneration after bridging sciatic nerve defect of rats, the effect is as good as that of the autograft nerve, significantly superior to the PRGD/PDLLA/β-TCP conduit and PDLLA conduit. PDLLA/PRGD/β-TCP/NGF composite conduit is a potential ideal conduit.展开更多
BACKGROUND 99mTc-3PRGD2.SPECT/CT is a commonly used examination method in nuclear medicine.However,patients receiving 99mTc-3PRGD2.SPECT/CT have insufficient knowledge of this method and worry about the examination re...BACKGROUND 99mTc-3PRGD2.SPECT/CT is a commonly used examination method in nuclear medicine.However,patients receiving 99mTc-3PRGD2.SPECT/CT have insufficient knowledge of this method and worry about the examination results.AIM To investigate the effect of teach-back health education combined with structured psychological nursing on adverse emotion and cooperation in patients undergoing 99mTc-3PRGD2.SPECT/CT examination.METHODS Ninety patients undergoing 99mTc-3PRGD2.SPECT/CT examinations were divided into a study group and a control group using a simple random number table,and 45 cases were allocated to each group.Routine nursing was provided to the control group,and teach-back health education combined with structured psychological nursing was provided to the study group on the basis of the control group.Heart rate,diastolic blood pressure,systolic blood pressure,self-rating depression scale(SDS),and self-rating anxiety scale(SAS)were assessed before and after the intervention,and examination cooperation and intervention satisfaction were assessed in the two groups before,during,and after the examination.RESULTS Before the examination,heart rate,diastolic blood pressure,and systolic blood pressure in the study group were not significantly different from the values of the control group(P>0.05).The results of the study group before and after the examination were lower than those in the control group(P<0.05).Before the intervention,SDS and SAS scores in the study group were not significantly different from those in the control group(P>0.05).After the intervention,SDS and SAS scores in the study group were lower than those in the control group(P<0.05).The degree of cooperation was higher in the study group than in the control group(P<0.05).The satisfaction rate with the intervention was higher in the study group than in the control group(P<0.05).CONCLUSION Teach-back health education combined with structured psychological nursing can help maintain the stability of blood pressure and heart rate,relieve negative emotions,and improve the satisfaction and cooperation of patients undergoing 99mTc-3PRGD2.SPECT/CT examinations.展开更多
The objective of this study was to evaluate the degradability and biocompatibility of a novel composite materials which was grafted with RGD and immobilized with NGF（PRGD/PDLLA/NGF）. The releasing of NGF, the biodeg...The objective of this study was to evaluate the degradability and biocompatibility of a novel composite materials which was grafted with RGD and immobilized with NGF（PRGD/PDLLA/NGF）. The releasing of NGF, the biodegradability and cell-biocompatibility of PRGD/PDLLA/NGF membrane were evaluated in vitro. The experimental results showed that the NGF release process was prolonged over 30 days. Furthermore, the PRGD/PDLLA/NGF showed a better hydrophilicity, better biodegradation properties and cells affinity than PDLLA, which means a good support to adhesion and proliferate of Schwann cells. Therefore, the novel composite material holds considerable promise as scaffolds in nerve tissue engineering.展开更多
Schwann cells play a key role in peripheral nerve growth and regeneration. The aim of this study was to evaluate the effects of RGD peptides on Schwann cell behavior, and to identify the effects of the modified PDLLA ...Schwann cells play a key role in peripheral nerve growth and regeneration. The aim of this study was to evaluate the effects of RGD peptides on Schwann cell behavior, and to identify the effects of the modified PDLLA films with RGD in vivo. The results revealed that RGD coating with the concentration of 100-500 ug/mL promoted the cell proliferation and boosted the cell migration. Molecularly, RGD coating also enhanced the expression of the proliferation related genes （c-fos and c-jun） and the cell behavior related genes （actin, tublin, tau and MAP1） at first stages of the seeding, which is similar to the effects from laminin coating. In vivo, RGD addition improved the recovery efficiency of the transected nerve in regard of the more survived Schwann cells in vivo and the formation of more mature myelin sheath. Taken together, RGD peptides are good candidates to enhance the biocompatibility of the biomaterials and facilitate the peripheral nerve regeneration by prompting responses in Schwann ceils.展开更多
Integrinα_(v)β_(3)is overexpressed in various tumor cells and angiogenesis.To date,no drug has been proven to target it for therapy.A first-in-human study was designed to investigate the safety,pharmacokinetics,and ...Integrinα_(v)β_(3)is overexpressed in various tumor cells and angiogenesis.To date,no drug has been proven to target it for therapy.A first-in-human study was designed to investigate the safety,pharmacokinetics,and dosimetry of^(177)Lu-AB-3PRGD2,a novel integrinα_(v)β_(3)-targeting radionuclide drug with an albumin-binding motif to optimize the pharmacokinetics.Ten patients(3 men,7 women;aged 45±16 years)with integrinα_(v)β_(3)-avid tumors were recruited to accept^(177)Lu-AB-3PRGD2 injection in a dosage of 1.57±0.08 GBq(42.32±2.11 mCi),followed by serial scans to obtain its dynamic distribution in the body.Safety tests were performed before and every 2 weeks after the treatment for 6-8 weeks.No adverse event over grade 3 was observed.^(177)Lu-AB-3PRGD2 was excreted mainly through the urinary system,with intense radioactivity in the kidneys and bladder.Moderate distribution was found in the liver,spleen,and intestines.The estimated blood half-life was 2.85±2.17 h.The whole-body effective dose was 0.251±0.047 mSv/MBq.The absorbed doses were 0.157±0.032 mGy/MBq in red bone marrow and 0.684±0.132 mGy/MBq in kidneys.This first-in-human study of^(177)Lu-AB-3PRGD2 treatment indicates its promising potential for targeted radionuclide therapy of integrinα_(v)β_(3)-avid tumors.It merits further studies in more patients with escalating doses and multiple treatment courses.展开更多
基金Funded by 973 State Key Fundamental Research and Development (No.G2005CB623905)
文摘Peripheral nerve regeneration using a novel nerve conduit （PRGD/PDLLA/β TCP/NGF） was evaluated, which was made of RGD peptide modified poly{（lactic acid）-co-[（glycolic acid）-alt-（L-lysine）]} （PRGD）, poly（d,l-lactic acid） （PDLLA） and β-tricalcium phosphate （β-TCP）. And the effectiveness was compared with that of PRGD/PDLLA/β-TCP, PDLLA and autograft in terms of nerve regeneration across a gap. Both of biodegradablity and cell-biocompatibility of the novel nerve conduit were evaluated in vitro. The results show that PRGD/PDLLA/β-TCP/NGF composite ma terials have better biodegradation properties and cell affinity than PDLLA, and could promote the RSC96 Schwann cells adhesion, proliferation and growth on the surface of materials. PRGD/PDLLA/ β-TCP/NGF composite conduit was significantly superior to that of the PDLLA conduit in histological and axon morphologic index. PRGD/PDLLA/β-TCP/NGF conduit is more beneficial to nerve regeneration than PDLLA conduit. The biodegradable PDLLA/PRGD/β-TCP/NGF conduit has a good biocompatibility with rats tissue and it could effectively promote the nerve regeneration after bridging sciatic nerve defect of rats, the effect is as good as that of the autograft nerve, significantly superior to the PRGD/PDLLA/β-TCP conduit and PDLLA conduit. PDLLA/PRGD/β-TCP/NGF composite conduit is a potential ideal conduit.
文摘BACKGROUND 99mTc-3PRGD2.SPECT/CT is a commonly used examination method in nuclear medicine.However,patients receiving 99mTc-3PRGD2.SPECT/CT have insufficient knowledge of this method and worry about the examination results.AIM To investigate the effect of teach-back health education combined with structured psychological nursing on adverse emotion and cooperation in patients undergoing 99mTc-3PRGD2.SPECT/CT examination.METHODS Ninety patients undergoing 99mTc-3PRGD2.SPECT/CT examinations were divided into a study group and a control group using a simple random number table,and 45 cases were allocated to each group.Routine nursing was provided to the control group,and teach-back health education combined with structured psychological nursing was provided to the study group on the basis of the control group.Heart rate,diastolic blood pressure,systolic blood pressure,self-rating depression scale(SDS),and self-rating anxiety scale(SAS)were assessed before and after the intervention,and examination cooperation and intervention satisfaction were assessed in the two groups before,during,and after the examination.RESULTS Before the examination,heart rate,diastolic blood pressure,and systolic blood pressure in the study group were not significantly different from the values of the control group(P>0.05).The results of the study group before and after the examination were lower than those in the control group(P<0.05).Before the intervention,SDS and SAS scores in the study group were not significantly different from those in the control group(P>0.05).After the intervention,SDS and SAS scores in the study group were lower than those in the control group(P<0.05).The degree of cooperation was higher in the study group than in the control group(P<0.05).The satisfaction rate with the intervention was higher in the study group than in the control group(P<0.05).CONCLUSION Teach-back health education combined with structured psychological nursing can help maintain the stability of blood pressure and heart rate,relieve negative emotions,and improve the satisfaction and cooperation of patients undergoing 99mTc-3PRGD2.SPECT/CT examinations.
文摘目的探讨^(99m)Tc-3PRGD2 SPECT/CT显像半定量参数对可疑乳腺病变的诊断价值,并根据乳腺癌分子分型进行分类比较,分析其与临床病理的相关性。方法收集2020年12月~2022年8月于我院接受检查的70例可疑乳腺病变患者病例资料。根据病理结果,分析乳腺癌Luminal阳性型、Her-2阳性型和三阴性型3种分子亚型与T/N值的相关性;分析雌激素受体、孕激素受体、Her-2及Ki67表达情况与影像学检查指标的关系。结果70例患者病理检查结果证实乳腺癌51例(72.86%),病理分型为Luminal阳性型22例,Her-2阳性型20例,三阴性型9例。乳腺良性病变24例(27.14%)。乳腺癌患者病变侧T/N值高于良性病变患者(3.96±0.82 vs 1.16±0.32),差异有统计学意义(t=14.426,P<0.05)。诊断特异性为84.21%(16/19),敏感度为82.35%(42/51),准确率为82.86%(58/70),ROC曲线下面积为0.834(0.755~0.913),诊断临界值为1.56,即T/N值≥1.56时判定为恶性病变。Her-2阳性型的T/N值高于三阴性型(P<0.05)。Her-2表达阳性患者的T/N值高于Her-2表达阴性的患者(P<0.05)。雌激素受体、孕激素受体和Ki67表达阳性和表达阴性患者的T/N值相比,差异均无统计学意义(P>0.05)。结论^(99m)Tc-3PRGD2 SPECT/CT显像半定量指标在诊断乳腺病变良恶性方面有较高的敏感度,并且Her-2阳性型患者T/N值明显升高,有助于早期发现和诊断乳腺病变,为预测乳腺癌分子分型和治疗有相应的指导作用。
基金Funded by the Key Project of Chinese National Programs for Fundamental Research and Development (No. 2011CB606205)the Self-determined and Innovative Research Funds of WUT (No. 2010-IV-042)
文摘The objective of this study was to evaluate the degradability and biocompatibility of a novel composite materials which was grafted with RGD and immobilized with NGF（PRGD/PDLLA/NGF）. The releasing of NGF, the biodegradability and cell-biocompatibility of PRGD/PDLLA/NGF membrane were evaluated in vitro. The experimental results showed that the NGF release process was prolonged over 30 days. Furthermore, the PRGD/PDLLA/NGF showed a better hydrophilicity, better biodegradation properties and cells affinity than PDLLA, which means a good support to adhesion and proliferate of Schwann cells. Therefore, the novel composite material holds considerable promise as scaffolds in nerve tissue engineering.
文摘Schwann cells play a key role in peripheral nerve growth and regeneration. The aim of this study was to evaluate the effects of RGD peptides on Schwann cell behavior, and to identify the effects of the modified PDLLA films with RGD in vivo. The results revealed that RGD coating with the concentration of 100-500 ug/mL promoted the cell proliferation and boosted the cell migration. Molecularly, RGD coating also enhanced the expression of the proliferation related genes （c-fos and c-jun） and the cell behavior related genes （actin, tublin, tau and MAP1） at first stages of the seeding, which is similar to the effects from laminin coating. In vivo, RGD addition improved the recovery efficiency of the transected nerve in regard of the more survived Schwann cells in vivo and the formation of more mature myelin sheath. Taken together, RGD peptides are good candidates to enhance the biocompatibility of the biomaterials and facilitate the peripheral nerve regeneration by prompting responses in Schwann ceils.
基金supported by Chinese Academy of Medical Science Innovation Fund for Medical Sciences(2022-I2M-2-002)the National High-Level Hospital Clinical Research Funding(2022-PUMCH-D-002,China)+2 种基金Clinical and translational medicine research project of the Chinese Academy of Medical Sciences(2022-I2M-C&T-A-008,China)the National Natural Science Foundation of China(82272046)the Fundamental Research Funds for the Central Universities(3332023124,China).
文摘Integrinα_(v)β_(3)is overexpressed in various tumor cells and angiogenesis.To date,no drug has been proven to target it for therapy.A first-in-human study was designed to investigate the safety,pharmacokinetics,and dosimetry of^(177)Lu-AB-3PRGD2,a novel integrinα_(v)β_(3)-targeting radionuclide drug with an albumin-binding motif to optimize the pharmacokinetics.Ten patients(3 men,7 women;aged 45±16 years)with integrinα_(v)β_(3)-avid tumors were recruited to accept^(177)Lu-AB-3PRGD2 injection in a dosage of 1.57±0.08 GBq(42.32±2.11 mCi),followed by serial scans to obtain its dynamic distribution in the body.Safety tests were performed before and every 2 weeks after the treatment for 6-8 weeks.No adverse event over grade 3 was observed.^(177)Lu-AB-3PRGD2 was excreted mainly through the urinary system,with intense radioactivity in the kidneys and bladder.Moderate distribution was found in the liver,spleen,and intestines.The estimated blood half-life was 2.85±2.17 h.The whole-body effective dose was 0.251±0.047 mSv/MBq.The absorbed doses were 0.157±0.032 mGy/MBq in red bone marrow and 0.684±0.132 mGy/MBq in kidneys.This first-in-human study of^(177)Lu-AB-3PRGD2 treatment indicates its promising potential for targeted radionuclide therapy of integrinα_(v)β_(3)-avid tumors.It merits further studies in more patients with escalating doses and multiple treatment courses.
