目的:探讨间充质干细胞对颞下关节炎患者血清PRG4及TGF-β1的软骨生成作用。方法:选取我院颞下颌关节炎患者72例,随机分为实验组和对照组。对照组予盐酸氨基葡萄糖片口服1个月,玻璃酸钠注射液颞下颌关节腔每周1次,注射4次治疗。实验组...目的:探讨间充质干细胞对颞下关节炎患者血清PRG4及TGF-β1的软骨生成作用。方法:选取我院颞下颌关节炎患者72例,随机分为实验组和对照组。对照组予盐酸氨基葡萄糖片口服1个月,玻璃酸钠注射液颞下颌关节腔每周1次,注射4次治疗。实验组予间充质干细胞培养、自体移植,生理盐水0.5 m L稀释细胞5×105个关节腔内注射,3天后再次相同剂量及方式注射。两组患者分别测定血清TGF-β1、IL-1α和PRG4蛋白表达,最大张口度测试及疼痛程度评分,并通过影像学检查,评估两组患者关节组织损伤修复时间。结果:1与对照组相比实验组TGF-β1和PRG4蛋白表达上升更迅速且维持在较高水平,IL-1α下降更显著,差异有统计学意义(P<0.05);2与对照组比较,实验组最大张口度增加、张口时疼痛评分下降,差异有统计学意义(P<0.05);3与对照组比较,实验组受损关节组织均修复时间更短,差异有统计学意义(P<0.05)。结论:间充质干细胞可通过调节血清TGF-β1、IL-1α水平和PRG4蛋白表达,促进软骨的生成,对改善预后,提高患者生活质量有重要意义。展开更多
The purpose of this study was to measure the amount of adsorption of various salivary proteins to a resin composite having various amounts of surface pre-reacted glass-ionomer (S-PRG) fillers, and to make a comparativ...The purpose of this study was to measure the amount of adsorption of various salivary proteins to a resin composite having various amounts of surface pre-reacted glass-ionomer (S-PRG) fillers, and to make a comparative study of the adherence of S. mutans to the resin composite covered by various salivary proteins. We experimentally produced resin composites (S-PRG resin) having the basic composition of Bis-GMA/TEGDMA and various amount of the S-PRG fillers ranging between 0 - 60 wt%. Each S-PRG resin block was soaked in 5 kinds of components found in salivary fluid (Mucin 1, Lactoferrin, IgA, Cystatin C, and Lysozyme), and the amount of adsorption was measured by use of a spectrophotometer. The amount of the adsorption of salivary Mucin 1 was higher than that of any other salivary component tested regardless of the percentage of the S-PRG filler. In the case of salivary Lysoxyme used for coating, the amount of its adsorption increased with an increase in the percentage of the S-PRG filler. In addition, resin blocks coated with various salivary proteins were incubated at 37℃ for 2 hours with radio-labeled S. mutans for a quantitative adherence test. Labeled bacteria that adhered to the resin blocks were collected by using an automatic sample combustion system and a liquid scintillation counter. The absorbed salivary components, especially Mucin 1 and Lysozyme, inhibited the adhesion of S. mutans to the S-PRG resin;however, these changes were generally directional rather than statistically significant.展开更多
文摘目的:探讨间充质干细胞对颞下关节炎患者血清PRG4及TGF-β1的软骨生成作用。方法:选取我院颞下颌关节炎患者72例,随机分为实验组和对照组。对照组予盐酸氨基葡萄糖片口服1个月,玻璃酸钠注射液颞下颌关节腔每周1次,注射4次治疗。实验组予间充质干细胞培养、自体移植,生理盐水0.5 m L稀释细胞5×105个关节腔内注射,3天后再次相同剂量及方式注射。两组患者分别测定血清TGF-β1、IL-1α和PRG4蛋白表达,最大张口度测试及疼痛程度评分,并通过影像学检查,评估两组患者关节组织损伤修复时间。结果:1与对照组相比实验组TGF-β1和PRG4蛋白表达上升更迅速且维持在较高水平,IL-1α下降更显著,差异有统计学意义(P<0.05);2与对照组比较,实验组最大张口度增加、张口时疼痛评分下降,差异有统计学意义(P<0.05);3与对照组比较,实验组受损关节组织均修复时间更短,差异有统计学意义(P<0.05)。结论:间充质干细胞可通过调节血清TGF-β1、IL-1α水平和PRG4蛋白表达,促进软骨的生成,对改善预后,提高患者生活质量有重要意义。
文摘The purpose of this study was to measure the amount of adsorption of various salivary proteins to a resin composite having various amounts of surface pre-reacted glass-ionomer (S-PRG) fillers, and to make a comparative study of the adherence of S. mutans to the resin composite covered by various salivary proteins. We experimentally produced resin composites (S-PRG resin) having the basic composition of Bis-GMA/TEGDMA and various amount of the S-PRG fillers ranging between 0 - 60 wt%. Each S-PRG resin block was soaked in 5 kinds of components found in salivary fluid (Mucin 1, Lactoferrin, IgA, Cystatin C, and Lysozyme), and the amount of adsorption was measured by use of a spectrophotometer. The amount of the adsorption of salivary Mucin 1 was higher than that of any other salivary component tested regardless of the percentage of the S-PRG filler. In the case of salivary Lysoxyme used for coating, the amount of its adsorption increased with an increase in the percentage of the S-PRG filler. In addition, resin blocks coated with various salivary proteins were incubated at 37℃ for 2 hours with radio-labeled S. mutans for a quantitative adherence test. Labeled bacteria that adhered to the resin blocks were collected by using an automatic sample combustion system and a liquid scintillation counter. The absorbed salivary components, especially Mucin 1 and Lysozyme, inhibited the adhesion of S. mutans to the S-PRG resin;however, these changes were generally directional rather than statistically significant.
