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槲皮素对肝硬化大鼠TLR4/CXCL9/PREX-2通路及肝功能的影响 被引量:6
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作者 许壮强 张文良 +2 位作者 吴文豪 吴飞 符书山 《中药材》 CAS 北大核心 2021年第8期1966-1970,共5页
目的:探究槲皮素对肝硬化大鼠肝功能及Toll样受体4(TLR4)/趋化因子9(CXCL9)/磷脂酰肌醇-3,4,5-三磷酸依赖的Rac交换因子2(PREX-2)通路相关蛋白表达的影响。方法:将SD大鼠随机分成正常对照组、模型组、甘利欣(0.0525 g/kg)阳性对照组及... 目的:探究槲皮素对肝硬化大鼠肝功能及Toll样受体4(TLR4)/趋化因子9(CXCL9)/磷脂酰肌醇-3,4,5-三磷酸依赖的Rac交换因子2(PREX-2)通路相关蛋白表达的影响。方法:将SD大鼠随机分成正常对照组、模型组、甘利欣(0.0525 g/kg)阳性对照组及槲皮素低(40 mg/kg)、中(60 mg/kg)、高(80 mg/kg)剂量组。除正常对照组外,其余各组均采用四氯化碳联合乙醇法建立肝硬化大鼠模型。各给药组给予相应剂量药物灌胃,1次/d,连续4 w。末次灌胃24 h后,酶联免疫吸附(ELISA)法检测血清中白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)含量;全自动生化分析仪检测肝功能指标丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)、白蛋白(ALB)和总胆红素(TBIL);化学发光法检测肝组织中纤维化指标透明质酸酶(HA)、层粘连蛋白(LN),Ⅲ型前胶原(PCⅢ),Ⅳ型胶原(Col-Ⅳ);苏木素-伊红(HE)染色检测肝脏组织病理学变化;蛋白印迹(Western blot)法检测肝脏组织中TLR4、CXCL9、PREX-2蛋白表达。结果:与正常对照组比较,模型组大鼠肝组织结构严重破坏,纤维组织增生,炎性细胞浸润,出现典型的假小叶,血清IL-6、IL-1β、TNF-α、ALT、AST、TBIL及肝脏组织HA、LN、PCⅢ、Col-Ⅳ水平和TLR4、CXCL9、PREX-2蛋白表达显著升高,血清ALB含量显著降低(P<0.05)。与模型组比较,各给药组大鼠肝组织结构基本完整,纤维组织增生明显减少,有少量炎性细胞浸润,未见假小叶,血清IL-6、IL-1β、TNF-α、ALT、AST、TBIL及肝脏组织HA、LN、PCⅢ、Col-Ⅳ水平和TLR4、CXCL9、PREX-2蛋白表达显著降低,血清ALB含量显著升高(P<0.05)。槲皮素的作用呈剂量依赖性。结论:槲皮素可能通过抑制TLR4/CXCL9/PREX-2通路减轻肝硬化大鼠炎症反应,缓解肝硬化进程,保护肝功能。 展开更多
关键词 槲皮素 肝硬化 TLR4/CXCL9/prex-2通路 肝功能
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Constraining the relativistic mean-field models from PREX-2 data:effective forces revisited
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作者 Jeet Amrit Pattnaik R.N.Panda +1 位作者 M.Bhuyan S.K.Patra 《Chinese Physics C》 SCIE CAS CSCD 2022年第9期218-231,共14页
Based on the current measurement of the neutron distribution radius(R_(n))of ^(208)Pb from the PREX-2 data,we revisited the recently developed G3 and IOPB-I force parameters by fine-tuning some specific couplings with... Based on the current measurement of the neutron distribution radius(R_(n))of ^(208)Pb from the PREX-2 data,we revisited the recently developed G3 and IOPB-I force parameters by fine-tuning some specific couplings within the relativistic mean-field(RMF)model.Theω-ρ-mesons coupling and theρ-meson coupling are constrained to the experimental neutron radius of^(208)Pb without compromising the bulk properties of finite nuclei and infinite nuclear matter observables.The modified parameter sets are applied to calculate the gross properties of finite nuclei such as binding energies,charge distributions,nuclear radii,pairing gaps,and single-particle energies.The root-mean-square deviations in binding energy and charge radius are estimated with respect to the available experimental data for 195 even-even nuclei,and the results compare favourably with the well-calibrated effective interactions of Skyrme,Gogny and other relativistic mean-field parametrizations.The pairing gap estimations for modified G3 and IOPB-I for Sn isotopes are also compared with the Hartree-Fock-Bogoliubov calculation with the Gogny(D1S)interaction.The isotopic shift and single-particle energy spacing are also calculated and compared with the experimental data for both original and modified versions of the G3 and IOPB-I parameter sets.Subsequently,both the modified parameter sets are used to obtain the various infinite nuclear matter observables at saturation.In addition to these,the force parameters are adopted to calculate the properties of a high isospin asymmetry dense system such as neutron star matter and tested for validation using the constraint from GW170817 binary neutron star merger events.The tuned forces predict relatively good results for finite and infinite nuclear matter systems and the current limitation on the neutron radius from PREX-2.A systematic analysis using these two refitted parameter sets over the nuclear chart will be communicated shortly. 展开更多
关键词 relativistic mean field model binding energy charge distribution radius neutron-skin thickness prex-2
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