Dear Editor,We recently reviewed two important studies that investigate the use of botulinum-A toxin(BoNT-A)injections into the bulbospongiosus muscle as a treatment for lifelong premature ejaculation(PE).While both s...Dear Editor,We recently reviewed two important studies that investigate the use of botulinum-A toxin(BoNT-A)injections into the bulbospongiosus muscle as a treatment for lifelong premature ejaculation(PE).While both studies share the goal of evaluating the efficacy and safety of BoNT-A in this context,they reached very different conclusions.The study by Shaher et al.demonstrated significant improvements in ejaculatory latency,indicating that BoNT-A injections may be a helpful treatment for PE.展开更多
Objective:To explore the positive role of developmental care measures in promoting the growth and development of premature infants,and analyze their specific effects on improving the sleep duration and quality of prem...Objective:To explore the positive role of developmental care measures in promoting the growth and development of premature infants,and analyze their specific effects on improving the sleep duration and quality of premature infants.Methods:A total of 80 premature infants who met the inclusion criteria were selected by convenient sampling method and divided into an experimental group and a control group,with 40 cases in each group.The experimental group received comprehensive nursing intervention of developmental care during the nursing process;the control group adopted the traditional conventional nursing model.Results:There was no statistically significant difference in various physical indicators between the two groups of premature infants before nursing intervention.After nursing intervention,the scores of intellectual development level,psychomotor development index and various physical indicators of premature infants in the experimental group were significantly higher than those in the control group,and the difference was statistically significant.Conclusion:The implementation of developmental care for premature infants can significantly improve their various developmental indicators,which can not only effectively promote the rapid development of premature infants’mental and physical growth,but also improve their sleep quality.展开更多
Objective:To explore the pharmacological effects and molecular mechanisms of a Jiao'ai decoction(JAD)in treating premature ovarian failure.Specifically,we evaluated the receptor for advanced glycation end(RAGE)pro...Objective:To explore the pharmacological effects and molecular mechanisms of a Jiao'ai decoction(JAD)in treating premature ovarian failure.Specifically,we evaluated the receptor for advanced glycation end(RAGE)products pathway,metabolic disorders,and intestinal flora dysbiosis.Methods:Forty female rats with normal estrous cycles were randomly divided into five groups:control,model,estradiol,low-dose JAD,and high-dose JAD,with 8 rats in each.Except for the control group,the rats in other groups were injected intraperitoneally with cisplatin for 8 days(1.5 mg/kg)to establish a premature ovarian failure model.Starting on the fifth day of cisplatin injections,the estradiol,low-dose JAD,and high-dose JAD groups were administered corresponding drugs for 21 days.Sex hormone levels and pathological changes in the ovaries were measured.Key proteins in the RAGE pathway related to apoptosis,aging,and inflammation,were tested using Western blot.A 16S rRNA analysis of feces and non-targeted metabolism in serum was performed to determine the effects of JAD on intestinal flora and metabolism.Results:Body weight,ovarian index,and the number of follicles at all levels increased in the JAD group.Regarding serum hormones,estradiol,anti-mullerian hormone(AMH)and P levels increased,whereas follicle-stimulating hormone(FSH)and luteinizing hormone(LH)levels decreased in the JAD group.The levels of phosphorylated Akt protein(P-Akt),receptor for advanced glycation end products(RAGE),tumor protein p53(P53),C-reactive protein(CRP),apoptosis regulator BAX(BAX)and Caspase3 were downregulated by JAD,whereas B cell leukemia/lymphoma 2(Bcl-2)and Endothelial nitric oxidase synthase(eNOS)were upregulated.JAD was also found to play an important role in the regulation of metabolic disorders and intestinal ecological imbalances by adjusting species composition and diversity.Conclusion:JAD can protect ovaries by exerting anti-inflammatory and anti-apoptotic effects via inhibition of the RAGE pathway.JAD can also regulate metabolic disorders and maintain the dynamic balance of intestinal flora,thereby contributing to the improvement of the ovarian reserve function.展开更多
Premature senescence in Bacillus thuringiensis(Bt)cotton has emerged as a significant challenge to the formation and realization of fiber yield and quality since its commercialization in 1997.Initially,premature senes...Premature senescence in Bacillus thuringiensis(Bt)cotton has emerged as a significant challenge to the formation and realization of fiber yield and quality since its commercialization in 1997.Initially,premature senescence was thought to be an inherent trait associated with the Bt gene.However,subsequent research and practice have demonstrated that it is not directly linked to the Bt gene but rather results from a physiological imbalance between the sink and source,as well as between the root and shoot in Bt cotton.This short review provides an overview of the causes,mechanisms,and control measures for premature senescence in Bt cotton.It offers valuable insights for future research and the sustainable application of transgenic crops.展开更多
A survey conducted on the premature bolting of Huarong large leaf mustard from 2018 to 2024 revealed that Huarong large leaf mustard sown in middle August was associated with a higher propensity for premature bolting....A survey conducted on the premature bolting of Huarong large leaf mustard from 2018 to 2024 revealed that Huarong large leaf mustard sown in middle August was associated with a higher propensity for premature bolting. Furthermore, it was observed that the earlier being sown, the greater the rate of premature bolting when being sown prior to middle August. The rate of premature bolting observed in seedlings sown on August 8 was recorded at 35.6%. It was noted that as the age of the seedlings increased, the rate of premature bolting correspondingly increased. There were notable differences in the tolerance of various cultivars to elevated temperatures and prolonged sunlight exposure. For instance, cultivars such as Zhangjie 1 and Sichuan Shaguodi, which exhibit greater heat resistance, did not demonstrate premature bolting when sown in early August. The prolonged exposure to elevated temperatures, drought conditions, and extended periods of sunlight during the seedling stage of Huarong large leaf mustard, coupled with delayed irrigation and transplantation, contributed to the occurrence of premature bolting. The Huarong large leaf mustard, when been sown from late August to early September and transplanted at the appropriate time, exhibited normal growth and development, with no instances of premature bolting observed. It is advisable to select heat-resistant varieties, such as Zhangjie 1, prior to middle August. Huarong large leaf mustard should be sown in early to middle September. Additionally, it is essential to ensure centralized production and timely release of seeds, prompt transplantation and harvesting, and enhance the management of pests and diseases.展开更多
Objective:To study the antioxidant protective effect and mechanism of melatonin on cyclophosphamide-induced premature ovarian failure model mice.Methods:Six-month sexually mature female Kunming mice were taken for one...Objective:To study the antioxidant protective effect and mechanism of melatonin on cyclophosphamide-induced premature ovarian failure model mice.Methods:Six-month sexually mature female Kunming mice were taken for one week of acclimatization and then randomly divided into a normal group,blank control group,drug control group,ovarian premature aging model group,and melatonin intervention low,medium,and high dose group,with 20 mice in each group.We observed the status and body mass of the mice in each group;observed and monitored the estrous cycle by HE staining;measured the diameter and size of the ovaries and weighed the wet weight of the ovaries;observed the morphological changes of the ovaries by HE staining and counted the developing follicles at all levels;detected the levels of serum estradiol(E2),follicle-stimulating hormone(FSH),and luteinizing hormone(LH)by ELISA;measured the levels of serum MDA,SOD,and GSH-PX by antioxidant kit;detected the levels of protein immunoblotting by ELISA;protein immunoblotting(Western blot)to examine the expression of DNA damage-related proteinsγH2AX,p53,and p21 in ovarian tissues.Results:Compared with the control group,mice in the premature ovarian failure model group showed reduced mobility,rough hair,decreased body weight,disorganized estrous cycle,decreased ovarian weight(P<0.05),decreased number of follicles at all levels of development(P<0.05),increased number of atretic follicles(P<0.05),significantly elevated levels of serum FSH and LH,significantly decreased levels of E2(P<0.05),significantly increased levels of serum MDA,significantly lower SOD and GSH-PX levels(P<0.05),and the expression of p53,p21,andγH2AX in ovarian tissues was increased(P<0.05).Compared with the model group of premature ovarian failure,melatonin improved the changes of the above indexes induced by cyclophosphamide-induced premature ovarian failure in mice.Conclusion:Melatonin can improve the changes of motility cycle disorders,abnormal follicular development,and abnormal serum hormone levels induced by cyclophosphamide-induced oxidative stress in mice with premature ovarian failure.At the same time,melatonin can improve the oxidative stress induced by cyclophosphamide and alleviate the role of oxidative stress-induced DNA damage in mouse ovaries by exerting its antioxidant effect.展开更多
Age-related osteoporosis poses a significant challenge in musculoskeletal health;a condition characterized by reduced bone density and increased fracture susceptibility in older individuals necessitates a better under...Age-related osteoporosis poses a significant challenge in musculoskeletal health;a condition characterized by reduced bone density and increased fracture susceptibility in older individuals necessitates a better understanding of underlying molecular and cellular mechanisms.Emerging evidence suggests that osteocytes are the pivotal orchestrators of bone remodeling and represent novel therapeutic targets for age-related bone loss.Our study uses the prematurely aged PolgD257A/D257A(PolgA)mouse model to scrutinize age-and sex-related alterations in musculoskeletal health parameters(frailty,grip strength,gait data),bone and particularly the osteocyte lacuno-canalicular network(LCN).Moreover,a new quantitative in silico image analysis pipeline is used to evaluate the alterations in the osteocyte network with aging.Our findings underscore the pronounced degenerative changes in the musculoskeletal health parameters,bone,and osteocyte LCN in PolgA mice as early as 40 weeks,with more prominent alterations evident in aged males.Our findings suggest that the PolgA mouse model serves as a valuable model for studying the cellular mechanisms underlying age-related bone loss,given the comparable aging signs and age-related degeneration of the bone and the osteocyte network observed in naturally aging mice and elderly humans.展开更多
Premature ovarian insufficiency(POI)is characterized by irreversible loss of ovarian function before 40 years of age and affects 3.7%of women worldwide(Golezar et al.,2019;Huang et al.,2021).Raised serum follicle-stim...Premature ovarian insufficiency(POI)is characterized by irreversible loss of ovarian function before 40 years of age and affects 3.7%of women worldwide(Golezar et al.,2019;Huang et al.,2021).Raised serum follicle-stimulating hormone(FSH)levels,menstrual disturbance,and estrogen deficiency are the main symptoms of POl(European Society for Human Reproduction et al.,2016).Besides infertility,patients have an increased risk of long-term complications due to the early deficiency of ovarian steroids,such as osteoporosis,cardiovascular disease,and metabolic disorders(lshizuka,2021).Due to the lack of biomarkers for prediction or early diagnosis,most patients are not diagnosed until the failure stage(serum levels of FSH above 25 IU/L and amenorrhea),missing opportunities for intervention.Therefore,developing sensitive biomarkers for diminished ovarian reserves is paramount for timely treatment to improve reproductive outcomes and prevent long-term complications.展开更多
Objective:To explore the correlation between night ECG parameters and sleep quality in elderly patients with atrial premature beat(PAC).Methods:A total of 307 elderly patients with PAC were selected from March 2022 to...Objective:To explore the correlation between night ECG parameters and sleep quality in elderly patients with atrial premature beat(PAC).Methods:A total of 307 elderly patients with PAC were selected from March 2022 to March 2024.The parameters of room morning load and heart rate variability(HRV)at night(22:00-6:00)were collected by 24h holter electrocardiogram,and the sleep quality of PAC patients was evaluated by Pittsburgh Sleep Quality Index(PSQI).Multiple regression analysis was used to explore the correlation between night ECG parameters and sleep quality.Results:The incidence of sleep disorder in 307 elderly PAC patients was 62.54%.Univariate analysis showed that there were no statistically significant differences in gender,BMI and education level(P>0.05),but there were statistically significant differences in age,disease course,underlying diseases,atrial morning load,SDNN,RMSSD,LF,HF,LF/HF and TP(P<0.05).Multivariate Logistic regression analysis showed that atrial morning load,SDNN,RMSSD,LF and LF/HF were independent influencing factors of sleep disorder in elderly PAC patients(P<0.05).Patients with sleep disorders were divided into mild group,moderate group and severe group according to PSQI score,and there were statistically significant differences in the indexes of atrial morning load,SDNN,LF,LF/HF and TP among the three groups(all P<0.05).Spearman correlation analysis showed that room morning load,LF,LF/HF and TP were positively correlated with the degree of sleep disorder,while SDNN parameters were negatively correlated with the degree of sleep disorder(all P<0.05).Conclusion:There is a significant correlation between night ECG parameters and sleep disorders in elderly patients with PAC.It is possible to prevent and treat sleep disorders by monitoring ECG abnormalities and improve the reliability of treatment.展开更多
Objective:To explore the application effect of improved“Bird’s nest”nursing in the transport of premature infants.Methods:Collecting 100 cases of premature infants,randomly divided into experimental group and contr...Objective:To explore the application effect of improved“Bird’s nest”nursing in the transport of premature infants.Methods:Collecting 100 cases of premature infants,randomly divided into experimental group and control group,two groups of premature 50 cases each.The control group received the traditional“Bird’s nest”nursing.Experimental group in modified the“bird’s nest”nursing intervention,compared two groups of premature monitoring vital signs during the transfer process,transport after the body development indicators,the time and cure warmers intervention.Results:Compared with control group,experimental group premature temperature fluctuation is small,apnea and fewer times of low body temperature and oxygen saturation is higher,the contrast results with statistical significance(P<0.05).There was no significant difference in physical development,time to get out of the incubator,and cure rate.Conclusion:Modified the“bird’s nest”care in outside the hospital application,help to improve premature transfer process safety,and security for premature success implementation of transshipment is of great significance.展开更多
Human norovirus(HuNoV)is the leading cause of acute gastroenteritis.The varying severity of chronic infection in patients with underlying immune deficiencies poses additional burdens on public health.However,the poten...Human norovirus(HuNoV)is the leading cause of acute gastroenteritis.The varying severity of chronic infection in patients with underlying immune deficiencies poses additional burdens on public health.However,the potential effects of HuNoV infection during pregnancy,a specific immune perturbed state,have been rarely reported.Recently,four cases of HuNoV-infected patients in the late stages of pregnancy were admitted to the Guangzhou Women and Children's Medical Center,and premature rupture of membranes as primary adverse outcome was observed in these cases.Samples of fetal accessory tissue were collected from two of these cases at delivery to explore the potential pathogenesis.Pathological analysis showed placental malperfusion in both maternal and fetal vascular,while a decrease in vessels was not observed in villi of placenta.There was obvious pathological change in the chorion of fetal membrane,accompanied by a tendency of Th-1 immune bias.Notably,aggregation of M2 macrophages was observed in the chorion of the fetal membrane,potentially recruited for tissue repair.Next-generation sequencing showed minimal changes in immune pathways within placenta tissue.A gene panel associated with immunosuppression was identified in the fetal membrane of HuNoV-infected women compared to those of normal parturient.Taken together,this study provides clues for the association between the HuNoV and premature delivery,which requires the attention of the clinicians.展开更多
BACKGROUND Premature ovarian insufficiency(POI)is a condition that causes secondary amenorrhea owing to ovarian hypofunction at an early stage.Early follicular depletion results in intractable infertility,thereby cons...BACKGROUND Premature ovarian insufficiency(POI)is a condition that causes secondary amenorrhea owing to ovarian hypofunction at an early stage.Early follicular depletion results in intractable infertility,thereby considerably reducing the quality of life of females.Given the continuum in weakened ovarian function,progressing from incipient ovarian failure(IOF)to transitional ovarian failure and further to POI,it is necessary to develop biomarkers for predicting POI.The oxidative stress states in IOF and POI were comprehensively evaluated via oxidative stress[diacron-reactive oxygen metabolites(d-ROMs)]test and anti-oxidant capacity[biological antioxidant potential(BAP)].METHODS Females presenting with secondary amenorrhea over 4 mo and a follicle stimulating hormone level of>40 mIU/mL were categorized into the POI group.Females presenting with a normal menstrual cycle and a follicle stimulating hormone level of>10.2 mIU/mL were categorized into the IOF group.Healthy females without ovarian hypofunction were categorized into the control group.Among females aged<40 years who visited our hospital from January 2021 to June 2022,we recruited 11 patients into both POI and IOF groups.For the potential antioxidant capacity,the relative oxidative stress index(BAP/d-ROMs×100)was calculated,and the oxidative stress defense system was comprehensively evaluated.RESULTS d-ROMs were significantly higher in the POI and IOF groups than in the control group,(478.2±58.7 U.CARR,434.5±60.6 U.CARR,and 341.1±35.1 U.CARR,respectively)(U.CARR is equivalent to 0.08 mg/dL of hydrogen peroxide).However,no significant difference was found between the POI and IOF groups.Regarding BAP,no significant difference was found between the control,IOF,and POI groups(2078.5±157.4μmol/L,2116.2±240.2μmol/L,and 2029.0±186.4μmol/L,respectively).The oxidative stress index was significantly higher in the POI and IOF groups than in the control group(23.7±3.3,20.7±3.6,and 16.5±2.1,respectively).However,no significant difference was found between the POI and IOF groups.CONCLUSION High levels of oxidative stress suggest that evaluating the oxidative stress state may be a useful indicator for the early detection of POI.展开更多
The incidence of premature ovarian insufficiency(POI)is increasing worldwide,particularly among younger women,posing a significant challenge to fertility.In addition to menopausal symptoms,POI leads to several complic...The incidence of premature ovarian insufficiency(POI)is increasing worldwide,particularly among younger women,posing a significant challenge to fertility.In addition to menopausal symptoms,POI leads to several complications that profoundly affect female reproductive function and overall health.Unfortunately,current clinical treatment strategies for this condition are limited and often yield unsatisfactory outcomes.These approaches typically involve hormone repla-cement therapy combined with psychological support.Recently,mesenchymal stem cell(MSC)therapies for POI have garnered considerable attention in global research.MSCs can restore ovarian reproductive and endocrine functions through diverse mechanisms,including controlling differentiation,promoting angiogenesis,regulating ovarian fibrosis,inhibiting apoptosis,enhancing autocrine and paracrine effects,suppressing inflammation,modulating the immune system,and genetic regulation.This editorial offers a succinct summary of the application of MSC therapy in the context of POI,providing evidence for groundbreaking medical approaches that have potential to enhance reproductive health and overall well-being for women.展开更多
OBJECTIVE:To analyze the serum metabolic targets of the"Zhibian(BL54)through Shuidao(ST28)"acupuncture technique in cyclophosphamide(CTX)-induced premature ovarian insufficiency(POI)model rats and to elucida...OBJECTIVE:To analyze the serum metabolic targets of the"Zhibian(BL54)through Shuidao(ST28)"acupuncture technique in cyclophosphamide(CTX)-induced premature ovarian insufficiency(POI)model rats and to elucidate the potential molecular mechanism of acupuncture in improving POI.METHODS:We used an intraperitoneal injection of CTX to establish the POI rat model(POI group)and compared serum hormone levels and ovarian histopathological changes to evaluate the effect of the Zhibian(BL54)through Shuidao(ST28)technique(ZS+POI group)on ovarian function.Then,nontargeted metabolomics was performed using rat serum by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF/MS).RESULTS:After acupuncture intervention,the serum hormone levels and ovarian pathological morphology of POI rats were effectively improved.Moreover,UPLC-Q-TOF/MS results showed that the ZS+POI group showed a significant reversal of the levels of 6 differential metabolites.Among them,the levels of four serum metabolic markers,divanillyltetrahydrofuran ferulate,trans-ferulic acid,tryptamine,and neuraminic acid,increased significantly.Further analysis of biological effects showed that all metabolites were involved in the regulation of reproductive hormone levels and antioxidant and antiapoptotic effects.CONCLUSIONS:The"Zhibian(BL54)through Shuidao(ST28)"acupuncture method may improve the ovarian function of POI rats by regulating serum metabolite markers to exert antioxidant and antiapoptotic effects,which provides a theoretical basis for the clinical application of acupuncture in the treatment of POI.展开更多
In a study of DNA methylation changes in melatonin-deficient rice mutants,mutant plants showed premature leaf senescence during grain-filling and reduced grain yield.Melatonin deficiency led to transcriptional reprogr...In a study of DNA methylation changes in melatonin-deficient rice mutants,mutant plants showed premature leaf senescence during grain-filling and reduced grain yield.Melatonin deficiency led to transcriptional reprogramming,especially of genes involved in chlorophyll and carbon metabolism,redox regulation,and transcriptional regulation,during dark-induced leaf senescence.Hypomethylation of mCG and mCHG in the melatonin-deficient rice mutants was associated with the expression change of both protein-coding genes and transposable element-related genes.Changes in gene expression and DNA methylation in the melatonin-deficient mutants were compensated by exogenous application of melatonin.A decreased S-adenosyl-L-methionine level may have contributed to the DNA methylation variations in rice mutants of melatonin deficiency under dark conditions.展开更多
AIM:To quantitatively assess the changes in mean vascular tortuosity(mVT)and mean vascular width(mVW)around the optic disc and their correlation with gestational age(GA)and birth weight(BW)in premature infants without...AIM:To quantitatively assess the changes in mean vascular tortuosity(mVT)and mean vascular width(mVW)around the optic disc and their correlation with gestational age(GA)and birth weight(BW)in premature infants without retinopathy of prematurity(ROP).METHODS:A single-center retrospective study included a total of 133(133 eyes)premature infants[mean corrected gestational age(CGA)43.6wk]without ROP as the premature group and 130(130 eyes)CGA-matched fullterm infants as the control group.The peripapillary mVT and mVW were quantitatively measured using computerassisted techniques.RESULTS:Premature infants had significantly higher mVT(P=0.0032)and lower mVW(P=0.0086)by 2.68(10^(4) cm^(-3))and 1.85μm,respectively.Subgroup analysis with GA showed significant differences(P=0.0244)in mVT between the early preterm and middle to late preterm groups,but the differences between mVW were not significant(P=0.6652).The results of the multiple linear regression model showed a significant negative correlation between GA and BW with mVT after adjusting sex and CGA(P=0.0211 and P=0.0006,respectively).For each day increase in GA at birth,mVT decreased by 0.1281(10^(4) cm^(-3))and for each 1 g increase in BW,mVT decreased by 0.006(10^(4) cm^(-3)).However,GA(P=0.9402)and BW(P=0.7275)were not significantly correlated with mVW.CONCLUSION:Preterm birth significantly affects the peripapillary vascular parameters that indicate higher mVT and narrower mVW in premature infants without ROP.Alterations in these parameters may provide new insights into the pathogenesis of ocular vascular disease.展开更多
Leaf senescence is the final stage of leaf development, where the nutrients and energy of senescent leaves are redistributed to developing tissues or organs for plant growth, reproduction, and defense. Outer leaves ar...Leaf senescence is the final stage of leaf development, where the nutrients and energy of senescent leaves are redistributed to developing tissues or organs for plant growth, reproduction, and defense. Outer leaves are photosynthetic organs that usually senesce at the late heading stage in Chinese cabbage, and premature leaf senescence often reduces leafy head yield and quality. In this study, 11 premature leaf senescence mutants were screened from an ethyl methanesulfonate-mutagenized population of the double haploid line ‘FT' in Chinese cabbage. At the early heading stage, the mutants exhibited edge yellowing within its outer leaves, and at the mature stage, its leafy head weight decreased significantly. Genetic analysis revealed that the mutated trait of all 11 mutants corresponds to single gene recessive inheritance. Semi-diallel cross tests showed that 5 of the 11 were allelic mutants. MutMap and Kompetitive Allele Specific PCR genotyping revealed that BraA01g001400.3C was the candidate gene, which is orthologous of Arabidopsis SUPPRESSOR OF rps4-RLD 1, encoding an immune regulator, so we named it as BrSRFR1. All the BrSRFR1 in the five allelic mutants exhibited single nucleotide polymorphisms at different positions on their exons and led to premature translation termination, which confirmed that defect in BrSRFR1 led to premature leaf senescence. These results verify the role of Br SRFR1 on leaf senescence and provide a new insight into the mechanisms of leaf senescence in Chinese cabbage, which reveals a novel function of SRFR1 in plant development.展开更多
Premature ovarian failure(POF)is a prevalent gynecological disorder with significant implications for the physical and mental well-being of affected individuals.Liu Wei Di Huang Wan(LWDHW),a Chinese herbal compound,ha...Premature ovarian failure(POF)is a prevalent gynecological disorder with significant implications for the physical and mental well-being of affected individuals.Liu Wei Di Huang Wan(LWDHW),a Chinese herbal compound,has demonstrated efficacy in alleviating the effects of POF.However,the underlying mechanism of action of LWDHW remains unclear.This study aimed to elucidate the potential molecular mechanism of LWDHW in treating POF using network pharmacology and molecular docking techniques.The active ingredients of LWDHW were initially screened through the TCMSP platform.At the same time,the relevant target genes associated with POF were identified using databases such as Disgenet,TTD,Drugbank,Gene Cards,OMIM,and Pharm GKB.Data analysis was conducted using the R language,Cytoscape,and STRING to construct and analyze the traditional Chinese medicine(TCM)regulatory network and protein-protein interaction(PPI)network maps.Subsequently,GO and KEGG enrichment analyses were performed using the R language.Finally,molecular docking was carried out between the protein receptors of the core genes and the corresponding small-molecule ligands.The study revealed 49 components and 189 predicted targets(after de-duplication)of LWDHW,along with 4524 targets(after de-duplication)associated with POF.Through comparative analysis,163 potential genes were identified as common targets of LWDHW and POF,participating in biological processes such as response to chemical substances,molecular function regulation,and signaling receptor binding.Key biological pathways implicated included the MAPK signaling pathway,IL-17 signaling pathway,and HIF-1 signaling pathway,among others.Molecular docking results demonstrated a robust binding ability between the core genes of LWDHW and their corresponding ingredients.In conclusion,this comprehensive analysis provided insights into the potential molecular mechanisms of LWDHW in treating POF.The identified common targets and associated pathways contributed to our understanding of how LWDHW exerted its therapeutic effects,paving the way for further research and clinical applications.It is worth noting that future studies with experimental validation and clinical trials are essential to confirm these findings and establish the safety and efficacy of LWDHW in the treatment of POF.展开更多
BACKGROUND The common cause of sodium nitrite poisoning has shifted from previous accidental intoxication by exposure or ingestion of contaminated water and food to recent alarming intentional intoxication as an emplo...BACKGROUND The common cause of sodium nitrite poisoning has shifted from previous accidental intoxication by exposure or ingestion of contaminated water and food to recent alarming intentional intoxication as an employed method of suicide/exit.The subsequent formation of methemoglobin(MetHb)restricts oxygen transport and utilization in the body,resulting in functional hypoxia at the tissue level.In clinical practice,a mismatch of cyanotic appearance and oxygen partial pressure usually contributes to the identification of methemoglobinemia.Prompt recognition of characteristic mismatch and accurate diagnosis of sodium nitrite poisoning are prerequisites for the implementation of standardized systemic interventions.CASE SUMMARY A pregnant woman was admitted to the Department of Critical Care Medicine at the First Affiliated Hospital of Harbin Medical University due to consciousness disorders and drowsiness 2 h before admission.Subsequently,she developed vomiting and cyanotic skin.The woman underwent orotracheal intubation,invasive mechanical ventilation(IMV),and correction of internal environment disturbance in the ICU.Her premature infant was born with a higher-than-normal MetHb level of 3.3%,and received detoxification with methylene blue and vitamin C,supplemental vitamin K1,an infusion of fresh frozen plasma,as well as respiratory support via orotracheal intubation and IMV.On day 3 after admission,the puerpera regained consciousness,evacuated the IMV,and resumed enteral nutrition.She was then transferred to the maternity ward 24 h later.On day 7 after admission,the woman recovered and was discharged without any sequelae.CONCLUSION MetHb can cross through the placental barrier.Level of MetHb both reflects severity of the sodium nitrite poisoning and serves as feedback on therapeutic effectiveness.展开更多
The gonadotropin-releasing hormone (GnRH) antagonist protocol has emerged as an efficacious alternative to the GnRH agonist protocol for controlled ovarian hyperstimulation (COH) during in vitro fertilization (IVF) cy...The gonadotropin-releasing hormone (GnRH) antagonist protocol has emerged as an efficacious alternative to the GnRH agonist protocol for controlled ovarian hyperstimulation (COH) during in vitro fertilization (IVF) cycles, and has been demonstrated applicability in infertile female patients with diverse ovarian responses. While the clinical implementation of the antagonist COH protocol has achieved widespread consensus, opportunities for refinement persist. Therefore, this review article focuses on the advantages and disadvantages of GnRH antagonist protocol, the selection of optimal standard doses, and the strategies for adjusting antagonist doses after the premature luteinizing hormone (LH) surge, aiming to provide more reasonable and scientific recommendations for the application of this scheme.展开更多
文摘Dear Editor,We recently reviewed two important studies that investigate the use of botulinum-A toxin(BoNT-A)injections into the bulbospongiosus muscle as a treatment for lifelong premature ejaculation(PE).While both studies share the goal of evaluating the efficacy and safety of BoNT-A in this context,they reached very different conclusions.The study by Shaher et al.demonstrated significant improvements in ejaculatory latency,indicating that BoNT-A injections may be a helpful treatment for PE.
文摘Objective:To explore the positive role of developmental care measures in promoting the growth and development of premature infants,and analyze their specific effects on improving the sleep duration and quality of premature infants.Methods:A total of 80 premature infants who met the inclusion criteria were selected by convenient sampling method and divided into an experimental group and a control group,with 40 cases in each group.The experimental group received comprehensive nursing intervention of developmental care during the nursing process;the control group adopted the traditional conventional nursing model.Results:There was no statistically significant difference in various physical indicators between the two groups of premature infants before nursing intervention.After nursing intervention,the scores of intellectual development level,psychomotor development index and various physical indicators of premature infants in the experimental group were significantly higher than those in the control group,and the difference was statistically significant.Conclusion:The implementation of developmental care for premature infants can significantly improve their various developmental indicators,which can not only effectively promote the rapid development of premature infants’mental and physical growth,but also improve their sleep quality.
基金sponsored by the National Natural Science Foundation of China(81673764)National Key R&D Program of China(022YFC3502301).
文摘Objective:To explore the pharmacological effects and molecular mechanisms of a Jiao'ai decoction(JAD)in treating premature ovarian failure.Specifically,we evaluated the receptor for advanced glycation end(RAGE)products pathway,metabolic disorders,and intestinal flora dysbiosis.Methods:Forty female rats with normal estrous cycles were randomly divided into five groups:control,model,estradiol,low-dose JAD,and high-dose JAD,with 8 rats in each.Except for the control group,the rats in other groups were injected intraperitoneally with cisplatin for 8 days(1.5 mg/kg)to establish a premature ovarian failure model.Starting on the fifth day of cisplatin injections,the estradiol,low-dose JAD,and high-dose JAD groups were administered corresponding drugs for 21 days.Sex hormone levels and pathological changes in the ovaries were measured.Key proteins in the RAGE pathway related to apoptosis,aging,and inflammation,were tested using Western blot.A 16S rRNA analysis of feces and non-targeted metabolism in serum was performed to determine the effects of JAD on intestinal flora and metabolism.Results:Body weight,ovarian index,and the number of follicles at all levels increased in the JAD group.Regarding serum hormones,estradiol,anti-mullerian hormone(AMH)and P levels increased,whereas follicle-stimulating hormone(FSH)and luteinizing hormone(LH)levels decreased in the JAD group.The levels of phosphorylated Akt protein(P-Akt),receptor for advanced glycation end products(RAGE),tumor protein p53(P53),C-reactive protein(CRP),apoptosis regulator BAX(BAX)and Caspase3 were downregulated by JAD,whereas B cell leukemia/lymphoma 2(Bcl-2)and Endothelial nitric oxidase synthase(eNOS)were upregulated.JAD was also found to play an important role in the regulation of metabolic disorders and intestinal ecological imbalances by adjusting species composition and diversity.Conclusion:JAD can protect ovaries by exerting anti-inflammatory and anti-apoptotic effects via inhibition of the RAGE pathway.JAD can also regulate metabolic disorders and maintain the dynamic balance of intestinal flora,thereby contributing to the improvement of the ovarian reserve function.
基金supported by National Key Research and Development Program of China(2024YFD2300221)China Agricultural Research System(CARS-15–15)+1 种基金Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences(CXGC2024D03)Dong Hezhong Studio for Popularization of Science and Technology in Salt Tolerant Industrial Crops(202228297).
文摘Premature senescence in Bacillus thuringiensis(Bt)cotton has emerged as a significant challenge to the formation and realization of fiber yield and quality since its commercialization in 1997.Initially,premature senescence was thought to be an inherent trait associated with the Bt gene.However,subsequent research and practice have demonstrated that it is not directly linked to the Bt gene but rather results from a physiological imbalance between the sink and source,as well as between the root and shoot in Bt cotton.This short review provides an overview of the causes,mechanisms,and control measures for premature senescence in Bt cotton.It offers valuable insights for future research and the sustainable application of transgenic crops.
基金Supported by Key R&D Projects of Hunan Provincial Department of Science and Technology"Study on Key Modern Processing Techniques and Product Development of Huarong Mustard"(2023NK2039).
文摘A survey conducted on the premature bolting of Huarong large leaf mustard from 2018 to 2024 revealed that Huarong large leaf mustard sown in middle August was associated with a higher propensity for premature bolting. Furthermore, it was observed that the earlier being sown, the greater the rate of premature bolting when being sown prior to middle August. The rate of premature bolting observed in seedlings sown on August 8 was recorded at 35.6%. It was noted that as the age of the seedlings increased, the rate of premature bolting correspondingly increased. There were notable differences in the tolerance of various cultivars to elevated temperatures and prolonged sunlight exposure. For instance, cultivars such as Zhangjie 1 and Sichuan Shaguodi, which exhibit greater heat resistance, did not demonstrate premature bolting when sown in early August. The prolonged exposure to elevated temperatures, drought conditions, and extended periods of sunlight during the seedling stage of Huarong large leaf mustard, coupled with delayed irrigation and transplantation, contributed to the occurrence of premature bolting. The Huarong large leaf mustard, when been sown from late August to early September and transplanted at the appropriate time, exhibited normal growth and development, with no instances of premature bolting observed. It is advisable to select heat-resistant varieties, such as Zhangjie 1, prior to middle August. Huarong large leaf mustard should be sown in early to middle September. Additionally, it is essential to ensure centralized production and timely release of seeds, prompt transplantation and harvesting, and enhance the management of pests and diseases.
基金2023 Special Project for Serving the National Development Strategy with Basic Scientific Research Fees from Central Universities(No.31920230188)2023 Northwestern Minzu University College-Level Innovation and Entrepreneurship Training Program(No.X202310742289)2024 National College Students’Innovation and Entrepreneurship Training Program(No.202410742005)。
文摘Objective:To study the antioxidant protective effect and mechanism of melatonin on cyclophosphamide-induced premature ovarian failure model mice.Methods:Six-month sexually mature female Kunming mice were taken for one week of acclimatization and then randomly divided into a normal group,blank control group,drug control group,ovarian premature aging model group,and melatonin intervention low,medium,and high dose group,with 20 mice in each group.We observed the status and body mass of the mice in each group;observed and monitored the estrous cycle by HE staining;measured the diameter and size of the ovaries and weighed the wet weight of the ovaries;observed the morphological changes of the ovaries by HE staining and counted the developing follicles at all levels;detected the levels of serum estradiol(E2),follicle-stimulating hormone(FSH),and luteinizing hormone(LH)by ELISA;measured the levels of serum MDA,SOD,and GSH-PX by antioxidant kit;detected the levels of protein immunoblotting by ELISA;protein immunoblotting(Western blot)to examine the expression of DNA damage-related proteinsγH2AX,p53,and p21 in ovarian tissues.Results:Compared with the control group,mice in the premature ovarian failure model group showed reduced mobility,rough hair,decreased body weight,disorganized estrous cycle,decreased ovarian weight(P<0.05),decreased number of follicles at all levels of development(P<0.05),increased number of atretic follicles(P<0.05),significantly elevated levels of serum FSH and LH,significantly decreased levels of E2(P<0.05),significantly increased levels of serum MDA,significantly lower SOD and GSH-PX levels(P<0.05),and the expression of p53,p21,andγH2AX in ovarian tissues was increased(P<0.05).Compared with the model group of premature ovarian failure,melatonin improved the changes of the above indexes induced by cyclophosphamide-induced premature ovarian failure in mice.Conclusion:Melatonin can improve the changes of motility cycle disorders,abnormal follicular development,and abnormal serum hormone levels induced by cyclophosphamide-induced oxidative stress in mice with premature ovarian failure.At the same time,melatonin can improve the oxidative stress induced by cyclophosphamide and alleviate the role of oxidative stress-induced DNA damage in mouse ovaries by exerting its antioxidant effect.
基金the European Research Council(ERC Advanced MechAGE-ERC-2016-ADG-741883)the Swiss National Science Foundation(no.188522).
文摘Age-related osteoporosis poses a significant challenge in musculoskeletal health;a condition characterized by reduced bone density and increased fracture susceptibility in older individuals necessitates a better understanding of underlying molecular and cellular mechanisms.Emerging evidence suggests that osteocytes are the pivotal orchestrators of bone remodeling and represent novel therapeutic targets for age-related bone loss.Our study uses the prematurely aged PolgD257A/D257A(PolgA)mouse model to scrutinize age-and sex-related alterations in musculoskeletal health parameters(frailty,grip strength,gait data),bone and particularly the osteocyte lacuno-canalicular network(LCN).Moreover,a new quantitative in silico image analysis pipeline is used to evaluate the alterations in the osteocyte network with aging.Our findings underscore the pronounced degenerative changes in the musculoskeletal health parameters,bone,and osteocyte LCN in PolgA mice as early as 40 weeks,with more prominent alterations evident in aged males.Our findings suggest that the PolgA mouse model serves as a valuable model for studying the cellular mechanisms underlying age-related bone loss,given the comparable aging signs and age-related degeneration of the bone and the osteocyte network observed in naturally aging mice and elderly humans.
基金supported by the National Key Research&Developmental Program of China(2022YFC2703800,2022YFC2703000)the National Natural Science Foundation of China(82125014,82371646,32070847)+1 种基金Basic Science Center Program of NSFC(31988101)the Natural Science Foundation of Shandong Province(ZR202105250005,ZR2022YQ69,tsqn202211371).
文摘Premature ovarian insufficiency(POI)is characterized by irreversible loss of ovarian function before 40 years of age and affects 3.7%of women worldwide(Golezar et al.,2019;Huang et al.,2021).Raised serum follicle-stimulating hormone(FSH)levels,menstrual disturbance,and estrogen deficiency are the main symptoms of POl(European Society for Human Reproduction et al.,2016).Besides infertility,patients have an increased risk of long-term complications due to the early deficiency of ovarian steroids,such as osteoporosis,cardiovascular disease,and metabolic disorders(lshizuka,2021).Due to the lack of biomarkers for prediction or early diagnosis,most patients are not diagnosed until the failure stage(serum levels of FSH above 25 IU/L and amenorrhea),missing opportunities for intervention.Therefore,developing sensitive biomarkers for diminished ovarian reserves is paramount for timely treatment to improve reproductive outcomes and prevent long-term complications.
文摘Objective:To explore the correlation between night ECG parameters and sleep quality in elderly patients with atrial premature beat(PAC).Methods:A total of 307 elderly patients with PAC were selected from March 2022 to March 2024.The parameters of room morning load and heart rate variability(HRV)at night(22:00-6:00)were collected by 24h holter electrocardiogram,and the sleep quality of PAC patients was evaluated by Pittsburgh Sleep Quality Index(PSQI).Multiple regression analysis was used to explore the correlation between night ECG parameters and sleep quality.Results:The incidence of sleep disorder in 307 elderly PAC patients was 62.54%.Univariate analysis showed that there were no statistically significant differences in gender,BMI and education level(P>0.05),but there were statistically significant differences in age,disease course,underlying diseases,atrial morning load,SDNN,RMSSD,LF,HF,LF/HF and TP(P<0.05).Multivariate Logistic regression analysis showed that atrial morning load,SDNN,RMSSD,LF and LF/HF were independent influencing factors of sleep disorder in elderly PAC patients(P<0.05).Patients with sleep disorders were divided into mild group,moderate group and severe group according to PSQI score,and there were statistically significant differences in the indexes of atrial morning load,SDNN,LF,LF/HF and TP among the three groups(all P<0.05).Spearman correlation analysis showed that room morning load,LF,LF/HF and TP were positively correlated with the degree of sleep disorder,while SDNN parameters were negatively correlated with the degree of sleep disorder(all P<0.05).Conclusion:There is a significant correlation between night ECG parameters and sleep disorders in elderly patients with PAC.It is possible to prevent and treat sleep disorders by monitoring ECG abnormalities and improve the reliability of treatment.
基金Health Commission of Guangxi Zhuang Autonomous Region,Self-Financing Scientific Research Subject(Project No.:Z20210327)。
文摘Objective:To explore the application effect of improved“Bird’s nest”nursing in the transport of premature infants.Methods:Collecting 100 cases of premature infants,randomly divided into experimental group and control group,two groups of premature 50 cases each.The control group received the traditional“Bird’s nest”nursing.Experimental group in modified the“bird’s nest”nursing intervention,compared two groups of premature monitoring vital signs during the transfer process,transport after the body development indicators,the time and cure warmers intervention.Results:Compared with control group,experimental group premature temperature fluctuation is small,apnea and fewer times of low body temperature and oxygen saturation is higher,the contrast results with statistical significance(P<0.05).There was no significant difference in physical development,time to get out of the incubator,and cure rate.Conclusion:Modified the“bird’s nest”care in outside the hospital application,help to improve premature transfer process safety,and security for premature success implementation of transshipment is of great significance.
基金supported by the National Natural Science Foundation of China(No.82241071,32370163&32400134)National Key Research and Development program(2019YFC0121905).
文摘Human norovirus(HuNoV)is the leading cause of acute gastroenteritis.The varying severity of chronic infection in patients with underlying immune deficiencies poses additional burdens on public health.However,the potential effects of HuNoV infection during pregnancy,a specific immune perturbed state,have been rarely reported.Recently,four cases of HuNoV-infected patients in the late stages of pregnancy were admitted to the Guangzhou Women and Children's Medical Center,and premature rupture of membranes as primary adverse outcome was observed in these cases.Samples of fetal accessory tissue were collected from two of these cases at delivery to explore the potential pathogenesis.Pathological analysis showed placental malperfusion in both maternal and fetal vascular,while a decrease in vessels was not observed in villi of placenta.There was obvious pathological change in the chorion of fetal membrane,accompanied by a tendency of Th-1 immune bias.Notably,aggregation of M2 macrophages was observed in the chorion of the fetal membrane,potentially recruited for tissue repair.Next-generation sequencing showed minimal changes in immune pathways within placenta tissue.A gene panel associated with immunosuppression was identified in the fetal membrane of HuNoV-infected women compared to those of normal parturient.Taken together,this study provides clues for the association between the HuNoV and premature delivery,which requires the attention of the clinicians.
文摘BACKGROUND Premature ovarian insufficiency(POI)is a condition that causes secondary amenorrhea owing to ovarian hypofunction at an early stage.Early follicular depletion results in intractable infertility,thereby considerably reducing the quality of life of females.Given the continuum in weakened ovarian function,progressing from incipient ovarian failure(IOF)to transitional ovarian failure and further to POI,it is necessary to develop biomarkers for predicting POI.The oxidative stress states in IOF and POI were comprehensively evaluated via oxidative stress[diacron-reactive oxygen metabolites(d-ROMs)]test and anti-oxidant capacity[biological antioxidant potential(BAP)].METHODS Females presenting with secondary amenorrhea over 4 mo and a follicle stimulating hormone level of>40 mIU/mL were categorized into the POI group.Females presenting with a normal menstrual cycle and a follicle stimulating hormone level of>10.2 mIU/mL were categorized into the IOF group.Healthy females without ovarian hypofunction were categorized into the control group.Among females aged<40 years who visited our hospital from January 2021 to June 2022,we recruited 11 patients into both POI and IOF groups.For the potential antioxidant capacity,the relative oxidative stress index(BAP/d-ROMs×100)was calculated,and the oxidative stress defense system was comprehensively evaluated.RESULTS d-ROMs were significantly higher in the POI and IOF groups than in the control group,(478.2±58.7 U.CARR,434.5±60.6 U.CARR,and 341.1±35.1 U.CARR,respectively)(U.CARR is equivalent to 0.08 mg/dL of hydrogen peroxide).However,no significant difference was found between the POI and IOF groups.Regarding BAP,no significant difference was found between the control,IOF,and POI groups(2078.5±157.4μmol/L,2116.2±240.2μmol/L,and 2029.0±186.4μmol/L,respectively).The oxidative stress index was significantly higher in the POI and IOF groups than in the control group(23.7±3.3,20.7±3.6,and 16.5±2.1,respectively).However,no significant difference was found between the POI and IOF groups.CONCLUSION High levels of oxidative stress suggest that evaluating the oxidative stress state may be a useful indicator for the early detection of POI.
基金Supported by the Cohort Construction Project of Peking University Third Hospital,No.BYSYDL2022013Clinical Key Project of Peking University Third Hospital,No.BYSY2023049+1 种基金Special Grant for Capital Health Research and Development,No.2022-2-4097and Funding from State Key Laboratory of Female Fertility Promotion,Center for Reproductive Medicine,Department of Obstetrics and Gynecology,Peking University Third Hospital,No.BYSYSZKF2023027.
文摘The incidence of premature ovarian insufficiency(POI)is increasing worldwide,particularly among younger women,posing a significant challenge to fertility.In addition to menopausal symptoms,POI leads to several complications that profoundly affect female reproductive function and overall health.Unfortunately,current clinical treatment strategies for this condition are limited and often yield unsatisfactory outcomes.These approaches typically involve hormone repla-cement therapy combined with psychological support.Recently,mesenchymal stem cell(MSC)therapies for POI have garnered considerable attention in global research.MSCs can restore ovarian reproductive and endocrine functions through diverse mechanisms,including controlling differentiation,promoting angiogenesis,regulating ovarian fibrosis,inhibiting apoptosis,enhancing autocrine and paracrine effects,suppressing inflammation,modulating the immune system,and genetic regulation.This editorial offers a succinct summary of the application of MSC therapy in the context of POI,providing evidence for groundbreaking medical approaches that have potential to enhance reproductive health and overall well-being for women.
基金the Research Grant from the Natural Science Research Program of Shanxi Province:Based on Ultra-Performance Liquid Chromatography-Mass Spectrometry,the effect of"Zhibian(BL54)through Shuidao(ST28)"Needle Method on Ovarian Metabolism and Apoptosis Pathways in rats with Premature Ovarian Insufficiency was Discussed(No.2022030211216)。
文摘OBJECTIVE:To analyze the serum metabolic targets of the"Zhibian(BL54)through Shuidao(ST28)"acupuncture technique in cyclophosphamide(CTX)-induced premature ovarian insufficiency(POI)model rats and to elucidate the potential molecular mechanism of acupuncture in improving POI.METHODS:We used an intraperitoneal injection of CTX to establish the POI rat model(POI group)and compared serum hormone levels and ovarian histopathological changes to evaluate the effect of the Zhibian(BL54)through Shuidao(ST28)technique(ZS+POI group)on ovarian function.Then,nontargeted metabolomics was performed using rat serum by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF/MS).RESULTS:After acupuncture intervention,the serum hormone levels and ovarian pathological morphology of POI rats were effectively improved.Moreover,UPLC-Q-TOF/MS results showed that the ZS+POI group showed a significant reversal of the levels of 6 differential metabolites.Among them,the levels of four serum metabolic markers,divanillyltetrahydrofuran ferulate,trans-ferulic acid,tryptamine,and neuraminic acid,increased significantly.Further analysis of biological effects showed that all metabolites were involved in the regulation of reproductive hormone levels and antioxidant and antiapoptotic effects.CONCLUSIONS:The"Zhibian(BL54)through Shuidao(ST28)"acupuncture method may improve the ovarian function of POI rats by regulating serum metabolite markers to exert antioxidant and antiapoptotic effects,which provides a theoretical basis for the clinical application of acupuncture in the treatment of POI.
基金supported by the National Natural Science Foundation of China(32100448,32070558,32061143030,32170636)Natural Science Foundation of Jiangsu Province(BK20210799)+2 种基金Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD),the Seed Industry Revitalization Project of Jiangsu Province(JBGS[2021]009)the Shanghai Science and Technology Agriculture Project([2022]No.1–6)the Project of Zhongshan Biological Breeding Laboratory(BM2022008-029)。
文摘In a study of DNA methylation changes in melatonin-deficient rice mutants,mutant plants showed premature leaf senescence during grain-filling and reduced grain yield.Melatonin deficiency led to transcriptional reprogramming,especially of genes involved in chlorophyll and carbon metabolism,redox regulation,and transcriptional regulation,during dark-induced leaf senescence.Hypomethylation of mCG and mCHG in the melatonin-deficient rice mutants was associated with the expression change of both protein-coding genes and transposable element-related genes.Changes in gene expression and DNA methylation in the melatonin-deficient mutants were compensated by exogenous application of melatonin.A decreased S-adenosyl-L-methionine level may have contributed to the DNA methylation variations in rice mutants of melatonin deficiency under dark conditions.
基金Supported by the Fundamental Research Funds for the Central Universities (No.WK2100000045)the National Natural Science Foundation of China (No.U19B2044)+1 种基金Hefei Health Care Commission 2022 Applied Medical Research Project (No.Hwk2022yb028)Zhejiang Lab Open Research Project (No.K2022QA0AB04).
文摘AIM:To quantitatively assess the changes in mean vascular tortuosity(mVT)and mean vascular width(mVW)around the optic disc and their correlation with gestational age(GA)and birth weight(BW)in premature infants without retinopathy of prematurity(ROP).METHODS:A single-center retrospective study included a total of 133(133 eyes)premature infants[mean corrected gestational age(CGA)43.6wk]without ROP as the premature group and 130(130 eyes)CGA-matched fullterm infants as the control group.The peripapillary mVT and mVW were quantitatively measured using computerassisted techniques.RESULTS:Premature infants had significantly higher mVT(P=0.0032)and lower mVW(P=0.0086)by 2.68(10^(4) cm^(-3))and 1.85μm,respectively.Subgroup analysis with GA showed significant differences(P=0.0244)in mVT between the early preterm and middle to late preterm groups,but the differences between mVW were not significant(P=0.6652).The results of the multiple linear regression model showed a significant negative correlation between GA and BW with mVT after adjusting sex and CGA(P=0.0211 and P=0.0006,respectively).For each day increase in GA at birth,mVT decreased by 0.1281(10^(4) cm^(-3))and for each 1 g increase in BW,mVT decreased by 0.006(10^(4) cm^(-3)).However,GA(P=0.9402)and BW(P=0.7275)were not significantly correlated with mVW.CONCLUSION:Preterm birth significantly affects the peripapillary vascular parameters that indicate higher mVT and narrower mVW in premature infants without ROP.Alterations in these parameters may provide new insights into the pathogenesis of ocular vascular disease.
基金supported by the National Natural Science Foundation of China (Grant No.31972405)Graduate Student Innovation Cultivation Project of Shenyang Agricultural University (Grant No.2021YCXB16)。
文摘Leaf senescence is the final stage of leaf development, where the nutrients and energy of senescent leaves are redistributed to developing tissues or organs for plant growth, reproduction, and defense. Outer leaves are photosynthetic organs that usually senesce at the late heading stage in Chinese cabbage, and premature leaf senescence often reduces leafy head yield and quality. In this study, 11 premature leaf senescence mutants were screened from an ethyl methanesulfonate-mutagenized population of the double haploid line ‘FT' in Chinese cabbage. At the early heading stage, the mutants exhibited edge yellowing within its outer leaves, and at the mature stage, its leafy head weight decreased significantly. Genetic analysis revealed that the mutated trait of all 11 mutants corresponds to single gene recessive inheritance. Semi-diallel cross tests showed that 5 of the 11 were allelic mutants. MutMap and Kompetitive Allele Specific PCR genotyping revealed that BraA01g001400.3C was the candidate gene, which is orthologous of Arabidopsis SUPPRESSOR OF rps4-RLD 1, encoding an immune regulator, so we named it as BrSRFR1. All the BrSRFR1 in the five allelic mutants exhibited single nucleotide polymorphisms at different positions on their exons and led to premature translation termination, which confirmed that defect in BrSRFR1 led to premature leaf senescence. These results verify the role of Br SRFR1 on leaf senescence and provide a new insight into the mechanisms of leaf senescence in Chinese cabbage, which reveals a novel function of SRFR1 in plant development.
基金Science and Technology Commission Joint Traditional Chinese Medicine Project of Chongqing,China(Grant No.2023MSXM166)。
文摘Premature ovarian failure(POF)is a prevalent gynecological disorder with significant implications for the physical and mental well-being of affected individuals.Liu Wei Di Huang Wan(LWDHW),a Chinese herbal compound,has demonstrated efficacy in alleviating the effects of POF.However,the underlying mechanism of action of LWDHW remains unclear.This study aimed to elucidate the potential molecular mechanism of LWDHW in treating POF using network pharmacology and molecular docking techniques.The active ingredients of LWDHW were initially screened through the TCMSP platform.At the same time,the relevant target genes associated with POF were identified using databases such as Disgenet,TTD,Drugbank,Gene Cards,OMIM,and Pharm GKB.Data analysis was conducted using the R language,Cytoscape,and STRING to construct and analyze the traditional Chinese medicine(TCM)regulatory network and protein-protein interaction(PPI)network maps.Subsequently,GO and KEGG enrichment analyses were performed using the R language.Finally,molecular docking was carried out between the protein receptors of the core genes and the corresponding small-molecule ligands.The study revealed 49 components and 189 predicted targets(after de-duplication)of LWDHW,along with 4524 targets(after de-duplication)associated with POF.Through comparative analysis,163 potential genes were identified as common targets of LWDHW and POF,participating in biological processes such as response to chemical substances,molecular function regulation,and signaling receptor binding.Key biological pathways implicated included the MAPK signaling pathway,IL-17 signaling pathway,and HIF-1 signaling pathway,among others.Molecular docking results demonstrated a robust binding ability between the core genes of LWDHW and their corresponding ingredients.In conclusion,this comprehensive analysis provided insights into the potential molecular mechanisms of LWDHW in treating POF.The identified common targets and associated pathways contributed to our understanding of how LWDHW exerted its therapeutic effects,paving the way for further research and clinical applications.It is worth noting that future studies with experimental validation and clinical trials are essential to confirm these findings and establish the safety and efficacy of LWDHW in the treatment of POF.
基金Supported by the National Natural Science Foundation of China,No.82372172the Key Research and Development Plan Project of Heilongjiang Province,No.GA23C007+3 种基金the Heilongjiang Province Postdoctoral Start-up Fund,No.LBH-Q20037the Research Project of Heilongjiang Provincial Health Commission,No.20231717010461the Special Fund for Clinical Research of Wu Jie-ping Medical Foundation,No.320.6750.2022-02-16the Scientific Research Innovation Fund of the First Affiliated Hospital of Harbin Medical University,No.2021M08.
文摘BACKGROUND The common cause of sodium nitrite poisoning has shifted from previous accidental intoxication by exposure or ingestion of contaminated water and food to recent alarming intentional intoxication as an employed method of suicide/exit.The subsequent formation of methemoglobin(MetHb)restricts oxygen transport and utilization in the body,resulting in functional hypoxia at the tissue level.In clinical practice,a mismatch of cyanotic appearance and oxygen partial pressure usually contributes to the identification of methemoglobinemia.Prompt recognition of characteristic mismatch and accurate diagnosis of sodium nitrite poisoning are prerequisites for the implementation of standardized systemic interventions.CASE SUMMARY A pregnant woman was admitted to the Department of Critical Care Medicine at the First Affiliated Hospital of Harbin Medical University due to consciousness disorders and drowsiness 2 h before admission.Subsequently,she developed vomiting and cyanotic skin.The woman underwent orotracheal intubation,invasive mechanical ventilation(IMV),and correction of internal environment disturbance in the ICU.Her premature infant was born with a higher-than-normal MetHb level of 3.3%,and received detoxification with methylene blue and vitamin C,supplemental vitamin K1,an infusion of fresh frozen plasma,as well as respiratory support via orotracheal intubation and IMV.On day 3 after admission,the puerpera regained consciousness,evacuated the IMV,and resumed enteral nutrition.She was then transferred to the maternity ward 24 h later.On day 7 after admission,the woman recovered and was discharged without any sequelae.CONCLUSION MetHb can cross through the placental barrier.Level of MetHb both reflects severity of the sodium nitrite poisoning and serves as feedback on therapeutic effectiveness.
基金Hainan Province Major Science and Technology Plan Projects(No.ZDKJ2021037,ZDKJ2017007)National Natural Science Foundation of China(No.81960283),and Co-funded by the Hainan Provincial Academician Innovation Platform Research Project and the Hainan Provincial Clinical Medicine Center Construction Project。
文摘The gonadotropin-releasing hormone (GnRH) antagonist protocol has emerged as an efficacious alternative to the GnRH agonist protocol for controlled ovarian hyperstimulation (COH) during in vitro fertilization (IVF) cycles, and has been demonstrated applicability in infertile female patients with diverse ovarian responses. While the clinical implementation of the antagonist COH protocol has achieved widespread consensus, opportunities for refinement persist. Therefore, this review article focuses on the advantages and disadvantages of GnRH antagonist protocol, the selection of optimal standard doses, and the strategies for adjusting antagonist doses after the premature luteinizing hormone (LH) surge, aiming to provide more reasonable and scientific recommendations for the application of this scheme.
