Objective: to analyze the effect of first aid treatment with pralidoxime injection on organophosphorus pesticide poisoned patients. Methods: dividing 60 organophosphorus pesticide poisoned patients in our hospital fro...Objective: to analyze the effect of first aid treatment with pralidoxime injection on organophosphorus pesticide poisoned patients. Methods: dividing 60 organophosphorus pesticide poisoned patients in our hospital from February 2017 to April 2021 into research group and control group according to random number table method, 30 cases in each group, and performing routine emergency treatment with pralidoxime injection intervention, performing intravenous drip to patients in control group, performing after-loading intravenous drip to patients in research group, and comparing and analyzing the first aid effect and improvement of myocardial enzyme level between two groups. Results: the resuscitation time and AchE activity recovery time of patients in research group were shorter than those in control group after first aid, and the levels of cTnI and CK were lower than those in control group after first aid, the differences were statistically significant (P < 0.05). Conclusion: on the basis of conventional emergency intervention for organophosphorus pesticide poisoned patients, after-loading pralidoxime injection intravenous infusion can effectively improve emergency treatment effect, and promote recovery of patients' resuscitation and myocardial enzyme level.展开更多
An improved acetylcholinesterase liquid crystal(LC) biosensor has been developed for the identification of organophosphates(OPs) by using a reactivator. When the acetylcholinesterases(AChEs) inhibited by different kin...An improved acetylcholinesterase liquid crystal(LC) biosensor has been developed for the identification of organophosphates(OPs) by using a reactivator. When the acetylcholinesterases(AChEs) inhibited by different kinds of OPs are reactived by a reactivator, the catalytic activity of AChEs can be recovered with different activation efficiency because of the different phosphorylation structures formed in the inhibited AChEs. Accordingly, the reactived AChEs can catalyze the hydrolysis of acetylthiocholine to generate thiocholine product in different degrees, which will result in different catalytic growth of AuNPs and further form distinct orientational response of LCs. Based on such a reactivation mechanism, the AChE LC biosensor with a simple, rapid and visual procedure achieves an obvious identification of three OPs pesticides, methamidophos, trichlorfon and paraoxon, by using a pralidoxime reactivator.展开更多
文摘Objective: to analyze the effect of first aid treatment with pralidoxime injection on organophosphorus pesticide poisoned patients. Methods: dividing 60 organophosphorus pesticide poisoned patients in our hospital from February 2017 to April 2021 into research group and control group according to random number table method, 30 cases in each group, and performing routine emergency treatment with pralidoxime injection intervention, performing intravenous drip to patients in control group, performing after-loading intravenous drip to patients in research group, and comparing and analyzing the first aid effect and improvement of myocardial enzyme level between two groups. Results: the resuscitation time and AchE activity recovery time of patients in research group were shorter than those in control group after first aid, and the levels of cTnI and CK were lower than those in control group after first aid, the differences were statistically significant (P < 0.05). Conclusion: on the basis of conventional emergency intervention for organophosphorus pesticide poisoned patients, after-loading pralidoxime injection intravenous infusion can effectively improve emergency treatment effect, and promote recovery of patients' resuscitation and myocardial enzyme level.
基金supported by the International Scientific and Technological Cooperation Projects of China(2012DFR40480)the National Natural Science Foundation of China(21175037,21277042 and J1210040)
文摘An improved acetylcholinesterase liquid crystal(LC) biosensor has been developed for the identification of organophosphates(OPs) by using a reactivator. When the acetylcholinesterases(AChEs) inhibited by different kinds of OPs are reactived by a reactivator, the catalytic activity of AChEs can be recovered with different activation efficiency because of the different phosphorylation structures formed in the inhibited AChEs. Accordingly, the reactived AChEs can catalyze the hydrolysis of acetylthiocholine to generate thiocholine product in different degrees, which will result in different catalytic growth of AuNPs and further form distinct orientational response of LCs. Based on such a reactivation mechanism, the AChE LC biosensor with a simple, rapid and visual procedure achieves an obvious identification of three OPs pesticides, methamidophos, trichlorfon and paraoxon, by using a pralidoxime reactivator.
