[Objectives]To detect the protective effects of six protopanaxatriols(PPTs)on hypoxia/reoxygenation(H/R)induced cardiomyocyte injury by different treatments.[Methods]The 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetra...[Objectives]To detect the protective effects of six protopanaxatriols(PPTs)on hypoxia/reoxygenation(H/R)induced cardiomyocyte injury by different treatments.[Methods]The 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide(MTT)assay was used for detecting the protective effects of six PPTs including ginsenoside Rg1,Re,Rf,Rg2,(R)Rh1 and(S)Rh1 on cell viability reduced by H/R in different treatments.And the adenosine triphosphate(ATP)content and mitochondrial membrane potential(MMP)were used for detecting the mitochondrial function change during PPTs treatment.[Results]Among six PPTs,ginsenoside Rg1,Re,Rf,Rg2 and(R)Rh1 at the concentration of 12.5μM significantly increased the cell survival when treated before and during H/R.These five PPTs also significantly increased the ATP content and MMP reduced by H/R in the same manner.In comparison,only Rg1 significantly increased the cell viability compared with H/R group by pretreating and treating the cells during hypoxia process.[Conclusions]Different treatments affect the protective effects of PPTs.When treated before and during H/R,ginsenoside Rg1,Re,Rf,Rg2 and(R)Rh1 protect the cardiomyocyte against H/R injury mitochondrial function,and only ginsenoside Rg1 has protective effects when treated before and during hypoxia process.展开更多
在医学论文中,“ppm、ppb、ppt”这类英文缩写常常被作者作为单位符号使用,但“ppm、ppb、ppt”既不是数学符号,更不是单位符号,只是表示数量份额的英文名词缩写(英文全称分别为parts per million、parts per billion、parts per trilli...在医学论文中,“ppm、ppb、ppt”这类英文缩写常常被作者作为单位符号使用,但“ppm、ppb、ppt”既不是数学符号,更不是单位符号,只是表示数量份额的英文名词缩写(英文全称分别为parts per million、parts per billion、parts per trillion)。在实际研究中,仪器测量的数值可能会以“ppm、ppb、ppt”形式给出结果,作者在撰写文章进行数据描述时则需对“ppm、ppb、ppt”进行换算。展开更多
在医学论文中,“ppm、ppb、ppt”这类英文缩写常常被作者作为单位符号使用,但“ppm、ppb、ppt”既不是数学符号,更不是单位符号,只是表示数量份额的英文名词缩写(英文全称分别为parts per million、parts per billion、parts per trilli...在医学论文中,“ppm、ppb、ppt”这类英文缩写常常被作者作为单位符号使用,但“ppm、ppb、ppt”既不是数学符号,更不是单位符号,只是表示数量份额的英文名词缩写(英文全称分别为parts per million、parts per billion、parts per trillion)。在实际研究中,仪器测量的数值可能会以“ppm、ppb、ppt”形式给出结果,作者在撰写文章进行数据描述时则需对“ppm、ppb、ppt”进行换算。展开更多
基金Supported by General Colleges and Universities Youth Innovative Talents Project of Guangdong Province(2019GKQNCX134)Guangdong Doctoral Workstation Funds。
文摘[Objectives]To detect the protective effects of six protopanaxatriols(PPTs)on hypoxia/reoxygenation(H/R)induced cardiomyocyte injury by different treatments.[Methods]The 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide(MTT)assay was used for detecting the protective effects of six PPTs including ginsenoside Rg1,Re,Rf,Rg2,(R)Rh1 and(S)Rh1 on cell viability reduced by H/R in different treatments.And the adenosine triphosphate(ATP)content and mitochondrial membrane potential(MMP)were used for detecting the mitochondrial function change during PPTs treatment.[Results]Among six PPTs,ginsenoside Rg1,Re,Rf,Rg2 and(R)Rh1 at the concentration of 12.5μM significantly increased the cell survival when treated before and during H/R.These five PPTs also significantly increased the ATP content and MMP reduced by H/R in the same manner.In comparison,only Rg1 significantly increased the cell viability compared with H/R group by pretreating and treating the cells during hypoxia process.[Conclusions]Different treatments affect the protective effects of PPTs.When treated before and during H/R,ginsenoside Rg1,Re,Rf,Rg2 and(R)Rh1 protect the cardiomyocyte against H/R injury mitochondrial function,and only ginsenoside Rg1 has protective effects when treated before and during hypoxia process.
文摘在医学论文中,“ppm、ppb、ppt”这类英文缩写常常被作者作为单位符号使用,但“ppm、ppb、ppt”既不是数学符号,更不是单位符号,只是表示数量份额的英文名词缩写(英文全称分别为parts per million、parts per billion、parts per trillion)。在实际研究中,仪器测量的数值可能会以“ppm、ppb、ppt”形式给出结果,作者在撰写文章进行数据描述时则需对“ppm、ppb、ppt”进行换算。
文摘在医学论文中,“ppm、ppb、ppt”这类英文缩写常常被作者作为单位符号使用,但“ppm、ppb、ppt”既不是数学符号,更不是单位符号,只是表示数量份额的英文名词缩写(英文全称分别为parts per million、parts per billion、parts per trillion)。在实际研究中,仪器测量的数值可能会以“ppm、ppb、ppt”形式给出结果,作者在撰写文章进行数据描述时则需对“ppm、ppb、ppt”进行换算。
