Tyrosine decarboxylase(TyDC)converts tyrosine to tyramine and plays a crucial role in secondary metabolite reactions,development,and stress responses in plants.Currently,the biological role of TyDC proteins from trees...Tyrosine decarboxylase(TyDC)converts tyrosine to tyramine and plays a crucial role in secondary metabolite reactions,development,and stress responses in plants.Currently,the biological role of TyDC proteins from trees is unknown.This study provided evidence showing that poplar PaTyDC4 functions in wood development and drought stress response.PaTyDC4 is preferentially expressed in wood-forming cells in stems.Overexpression of PaTyDC4 in poplars under the control of a xylem-specific promoter resulted in an increase in the ratio of xylem to phloem width,vessel cell area,and lignin accumulation in the stems.Biochemical assays revealed that PaTyDC4 was a component of the PaC3H17-PaMYB199 module-mediated pathway.In poplar stems,Pa TyDC4 expression was directly suppressed by PaMYB199,which was attenuated by the interaction between PaC3H17 and PaMYB199.In addition,Pa TyDC4 overexpression lines showed stronger drought tolerance than the wild-type lines,with higher photosynthetic capacity and lower levels of H_(2)O_(2).These results indicate that PaTyDC4 promotes xylem differentiation and lignin deposition during secondary growth and confers drought tolerance.Our findings may be useful for the genetic modification of biomass and drought resistance in trees.展开更多
Short rotation plantation forestry(SRF)is being widely adopted to increase wood production,in order to meet global demand for wood products.However,to ensure maximum gains from SRF,optimised management regimes need to...Short rotation plantation forestry(SRF)is being widely adopted to increase wood production,in order to meet global demand for wood products.However,to ensure maximum gains from SRF,optimised management regimes need to be established by integrating robust predictions and an understanding of mechanisms underlying tree growth.Hybrid ecophysiological models,such as potentially useable light sum equation(PULSE)models,are useful tools requiring minimal input data that meet the requirements of SRF.PULSE models have been tested and calibrated for different evergreen conifers and broadleaves at both juvenile and mature stages of tree growth with coarse soil and climate data.Therefore,it is prudent to question:can adding detailed soil and climatic data reduce errors in this type of model?In addition,PULSE techniques have not been used to model deciduous species,which are a challenge for ecophysiological models due to their phenology.This study developed a PULSE model for a clonal Populus tomentosa plantation in northern China using detailed edaphic and climatic data.The results showed high precision and low bias in height(m)and basal area(m^(2)·ha^(-1))predictions.While detailed edaphoclimatic data produce highly precise predictions and a good mechanistic understanding,the study suggested that local climatic data could also be employed.The study showed that PULSE modelling in combination with coarse level of edaphic and local climate data resulted in reasonably precise tree growth prediction and minimal bias.展开更多
With the rapid development of nuclear energy,the removal of radioactive iodine generated during spent fuel reprocessing has become increasingly important.Based on the unique straw-like structure of populus tomentosa f...With the rapid development of nuclear energy,the removal of radioactive iodine generated during spent fuel reprocessing has become increasingly important.Based on the unique straw-like structure of populus tomentosa fiber(PTF)and the highly active iodine vapor capture ability of zero-valent silver nanoparticles(PTF@Ag^(0)NP),an Ag^(0)NP composite functional material with highly efficient iodine vapor capture capability was synthesized from biowaste PTF through ultrasonic and hightemperature hydrothermal methods in this study.The iodine capture experiment demonstrated that PTF@Ag^(0)NP exhibits rapid iodine capture efficiency,reaching dynamic equilibrium within 4 h and a maximum capture capacity of 1008.1 mg/g.Density functional theory calculations show that PTF@Ag^(0)NP exhibits extremely high chemical reactivity toward iodine,with a reaction binding energy of-2.88 e V.Additionally,the molecular dynamics of PTF@Ag^(0)NP indicate that there is no atomic displacement at 77?C,indicating the excellent temperature stability of the material at the operating temperature.The capture mechanism suggests that iodine vapor primarily reacts with Ag^(0)NP to form Ag I,and that the hydroxyl groups in PTF can also effectively capture iodine vapor by adsorption induction.In conclusion,PTF@Ag^(0)NP is expected to be an effective candidate adsorbent material for removing radioactive iodine vapor from exhaust gases during spent fuel reprocessing.展开更多
The addition of ectomycorrhizal fungi(ECMF),beneficial rhizosphere microorganisms,to the soil can pro-mote plant growth and resistance.Here,Populus davidi-ana×Populus bolleana tissue culture seedlings were grown ...The addition of ectomycorrhizal fungi(ECMF),beneficial rhizosphere microorganisms,to the soil can pro-mote plant growth and resistance.Here,Populus davidi-ana×Populus bolleana tissue culture seedlings were grown for 3 months in soils inoculated with one of the species,then seedlings were assessed for mycorrhizal colonization rate and growth,physiological and root traits.Suillus luteus and Populus involutus each formed ectomycorrhizal associa-tions with the seedlings.Seedling height,ground diameter,biomass,and leaf area were significantly greater after treat-ment with ECMF than in the non-inoculated controls.Treat-ment improved all physiological and root variables assessed(chlorophylls and carotenoids,cellulose,and soluble sugars and proteins;root length,surface area,projected area,mean diameter,volume,number of root tips).Seedlings inocu-lated with S.luteus outperformed those inoculated with P.involutus.展开更多
Populus alba‘Berolinensis’is a fast-growing,high-yielding species with strong biotic and abiotic stress resistance,and widely planted for timber,shelter belts and aesthetic purposes.In this study,molecular developme...Populus alba‘Berolinensis’is a fast-growing,high-yielding species with strong biotic and abiotic stress resistance,and widely planted for timber,shelter belts and aesthetic purposes.In this study,molecular development is explored and the important genes regulating xylem forma-tion in P.alba‘Berolinensis’under artificial bending treat-ments was identified.Anatomical investigation indicated that tension wood(TW)was characterized by eccentric growth of xylem and was enriched in cellulose;the degree of ligni-fication was lower than for normal wood(NW)and oppo-site wood(OW).RNA-Seq-based transcriptome analysis was performed using developing xylem from three wood types(TW,OW and NW).A large number of differentially expressed genes(DEGs)were screened and 4889 counted.In GO and KEGG enrichment results,genes involved in plant hormone signal transduction,phenylpropanoid biosynthesis,and cell wall and secondary cell wall biogenesis play major roles in xylem development under artificial bending.Eight expansin(PalEXP)genes were identified from the RNA-seq data;four were differentially expressed during tension wood formation.Phylogenetic analysis indicated that PalEXLB1 belongs to the EXPB subfamily and that the other PalEXPs are members of the EXPA subfamily.A transcriptional regulatory network construction showed 10 transcription factors located in the first and second layers upstream of EXP,including WRKY,ERF and bHLH.RT‒qPCR analy-sis in leaves,stems and roots combined with transcriptome analysis suggests that PalEXPA2,PalEXPA4 and PalEXPA15 play significant regulatory roles in cell wall formation during tension wood development.The candidate genes involved in xylem cell wall development during tension wood formation marks an important step toward identifying the molecular regulatory mechanism of xylem development and wood property improvement in P.alba‘Berolinensis’.展开更多
Leaves are important‘source’organs that synthesize organic matter,providing carbon sources for plant growth.Here,we used Populus talassica×Populus euphratica,the dominant species in ecological and timber forest...Leaves are important‘source’organs that synthesize organic matter,providing carbon sources for plant growth.Here,we used Populus talassica×Populus euphratica,the dominant species in ecological and timber forests,to simulate carbon limitation through artificial 25%,50%,and 75%defoliation treatments and explore the effects on root,stem,and leaf morphology,biomass accumulation,and carbon allocation strategies.At the 60th d after treat-ment,under 25%defoliation treatment,the plant height,specific leaf weight,root surface area and volume,and concentrations of non-structural carbohydrates in stem and root were significantly increased by 9.13%,20.00%,16.60%,31.95%,5.12%,and 9.34%,respectively,relative to the control.There was no significant change in the growth indicators under 50%defoliation treatment,but the concentrations of non-structural carbohydrates in the leaf and stem significantly decreased,showing mostly a negative correlation between them.The opposite was observed in the root.Under 75%defoliation treatment,the plant height,ground diameter,leaf number,single leaf area,root,stem,and total biomass were significantly reduced by 14.15%,10.24%,14.86%,11.31%,11.56%,21.87%,and 16.82%,respectively,relative to the control.The concentrations of non-structural carbohydrates in various organs were significantly reduced,particularly in the consumption of the starch concentrations in the stem and root.These results indicated that carbon allocation strategies can be adjusted to increase the con-centration of non-structural carbohydrates in root and meet plant growth needs under 25%and 50%defoliation.However,75%defoliation significantly limited the distribution of non-structural carbohydrates to roots and stems,reduced carbon storage,and thus inhibited plant growth.Defoliation-induced carbon limitation altered the carbon allocation pattern of P.talassica×P.euphratica,and the relationship between carbon reserves in roots and tree growth recovery after defoliation was greater.This study provides a theoretical basis for the comprehen-sive management of P.talassica×P.euphratica plantations,as well as a reference for the study of plantation car-bon allocation strategies in the desert and semi-desert regions of Xinjiang under carbon-limitation conditions.展开更多
As an important ecological tree species in northern China, Populus simonii plays a crucial role in maintaining ecological balance and promoting environmental sustainability. The academic community has conducted a seri...As an important ecological tree species in northern China, Populus simonii plays a crucial role in maintaining ecological balance and promoting environmental sustainability. The academic community has conducted a series of in-depth studies on this species, covering key areas such as genomics, survival mechanisms, and genetic breeding. Through the analysis of the genomic structure and function of P. simonii, we have not only revealed the molecular basis for its adaptation to harsh environments but also identified key genes that promote its growth and resistance to pests and diseases. Furthermore, exploring the survival mechanisms of P. simonii has deepened our understanding of its stress resistance traits, including how it effectively copes with abiotic stresses such as drought, salinization, and heavy metal pollution. In genetic breeding, significant progress has been made through the application of modern biotechnology, improving the growth rate and wood quality of P. simonii and enhancing its environmental adaptability and disease resistance. These research findings have not only enriched our knowledge of the biological characteristics of P. simonii but also provided a solid scientific foundation for its application in ecological restoration, forestry production, and environmental management.展开更多
Anoplophora glabripennis is one of the most devastating wood-boring beetles that attacks poplars.However,one poplar species,Populus deltoides,has strong resistance to Anoplophora glabripennis infestation,the underlyin...Anoplophora glabripennis is one of the most devastating wood-boring beetles that attacks poplars.However,one poplar species,Populus deltoides,has strong resistance to Anoplophora glabripennis infestation,the underlying defense mechanisms against Anoplophora glabripennis are poorly understood.Secondary metabolites play a crucial role in plants to combat biological stress.Here,based on transcriptome and metabolome,we demonstrated that the mechanisms for responses to mechanical damage and insect infestation were different.The degree of reactions to adult groove production,larval incubation,and larval frass production was not identical.In addition,the potential genes with insect resistance activity were identified.Predominant differentially expressed genes(DEGs)found in the phloem of Populus deltoides include anthocyanidin 3-O-glucosyltransferase5(PdUGT72E),peroxidase 73(PdPod73),peroxidase A2(PdPodA2)and macrophage migration inhibitory factor(PdMIF)responded to stress caused by Anoplophora glabripennis,which further resulted in activation of the plant defense system against insects via changes in regulation of metabolic pathways,such as tyrosine metabolism pathway,phenylpropanoid biosynthesis pathway and flavonoid biosynthesis pathway.Therefore,this work has laid a foundation for further unraveling the mechanisms involved in this interaction.展开更多
This study presented an improved CTAB method for extracting DNA from leaves of Populus euphratica Oliv. and Populus pruinosa Schrenk. based on the conventional CTAB method. The results showed that preventing DNA from ...This study presented an improved CTAB method for extracting DNA from leaves of Populus euphratica Oliv. and Populus pruinosa Schrenk. based on the conventional CTAB method. The results showed that preventing DNA from browning is a key step to obtain the high-quality DNA during DNA extraction, and under the condition of grinding in the presence of liquid nitrogen, adding such three antioxidants as PVP dry powder, Vc and β-mercaptoethanol could prevent DNA from browning effectively. The total DNA extracted by the improved CTAB method was subjected to PCR detection which proved that it totally satisfied the requirements of subsequent study.展开更多
The physical-mechanical properties of Populus x canadensis Moench and Populus x euramericana （Dode） Guinier cv. Gelrica were studied to provide theoret- ical and scientific bases for the directional breeding and eff...The physical-mechanical properties of Populus x canadensis Moench and Populus x euramericana （Dode） Guinier cv. Gelrica were studied to provide theoret- ical and scientific bases for the directional breeding and efficient use of artificial forests with P. canadensis and P. euramericana Gelrica. The results showed the air-dried density, basic density of P. canadensis were 0.468 g/cm3 and 0.372 g/cm3, respectively; the shrinkage coefficient of radial, tangential and volume were 0.133%, 0.270% and 0.553%, respectively;the modulus of elasticity in static bending, the bending strength and the compressive strength parallel to grain were 9 302.99 MPa, 79.69 MPa and 40.32 Mpa, respectively. As for the P. euramericana Gelrica, the air-dried density, basic densitywere 0.453 and 0.355 g/cm3, respectively; the shrink- age coefficient of radial, tangential and volume were 0.205%, 0.304% and 0.554%, respectively; the modulus of elasticity in static bending, the bending strength and the compressive strength parallel to grain were 9 014.44, 55.87 and 33.09 Mpa respectively. Comprehensive analysis of the indicators showed that the properties of P. canadensis were better than those of P.euramericana Gelrica.展开更多
cDNA encoding caffeoyl CoA O-methyltransferase (CCoAOMT) from Chinese white poplar ( Populus tomentosa Carr.) was cloned by RT-PCR and sequenced. Northern analysis displayed that the CCoAOMT was expressed specifically...cDNA encoding caffeoyl CoA O-methyltransferase (CCoAOMT) from Chinese white poplar ( Populus tomentosa Carr.) was cloned by RT-PCR and sequenced. Northern analysis displayed that the CCoAOMT was expressed specifically in the developing secondary xylem and its expression was coincident with lignification. The antisense CCoAOMT cDNA was transformed into P. tremula x P. alba mediated by Agrobacterium tumefaciens ( Smith et Townsend) Conn. Transgenic plants were identified with PCR, PCR-Southern and Southern analysis. Lignin content in 5- to 6-month-old transgenic plants was measured. One of the transgenic lines had significant reduction of 17.9% in Klason lignin content as compared with that of untransformed poplar. The results demonstrate that antisense repression of CCoAOMT is an efficient way to reduce lignin content for improving pulping property in engineered trees.展开更多
基金Financial support was obtained from National Natural Science Foundation of China(Grant Nos.32101549 and 32201585)Natural Science Foundation of Shandong Province,China(Grant No.ZR202112010288)+2 种基金Science&Technology Specific Projects in Agricultural High-tech Industrial Demonstration Area of the Yellow River Delta(Grant No.2022SZX39)Technical Innovation and Application Development Special Project of Chongqing(Grant No.CSTB2022TIAD-LDX0013)the Taishan Scholar Program of Shandong(Grant No.tsqn202103092)。
文摘Tyrosine decarboxylase(TyDC)converts tyrosine to tyramine and plays a crucial role in secondary metabolite reactions,development,and stress responses in plants.Currently,the biological role of TyDC proteins from trees is unknown.This study provided evidence showing that poplar PaTyDC4 functions in wood development and drought stress response.PaTyDC4 is preferentially expressed in wood-forming cells in stems.Overexpression of PaTyDC4 in poplars under the control of a xylem-specific promoter resulted in an increase in the ratio of xylem to phloem width,vessel cell area,and lignin accumulation in the stems.Biochemical assays revealed that PaTyDC4 was a component of the PaC3H17-PaMYB199 module-mediated pathway.In poplar stems,Pa TyDC4 expression was directly suppressed by PaMYB199,which was attenuated by the interaction between PaC3H17 and PaMYB199.In addition,Pa TyDC4 overexpression lines showed stronger drought tolerance than the wild-type lines,with higher photosynthetic capacity and lower levels of H_(2)O_(2).These results indicate that PaTyDC4 promotes xylem differentiation and lignin deposition during secondary growth and confers drought tolerance.Our findings may be useful for the genetic modification of biomass and drought resistance in trees.
基金The National Key Research and Development Program of China(Grant No.2021YFD2201203)the 5·5 Engineering Research&Innovation Team Project of Beijing Forestry University(No.BLRC2023C05)the Key Research and Development Program of Shandong Province(No.2021SFGC02050102)。
文摘Short rotation plantation forestry(SRF)is being widely adopted to increase wood production,in order to meet global demand for wood products.However,to ensure maximum gains from SRF,optimised management regimes need to be established by integrating robust predictions and an understanding of mechanisms underlying tree growth.Hybrid ecophysiological models,such as potentially useable light sum equation(PULSE)models,are useful tools requiring minimal input data that meet the requirements of SRF.PULSE models have been tested and calibrated for different evergreen conifers and broadleaves at both juvenile and mature stages of tree growth with coarse soil and climate data.Therefore,it is prudent to question:can adding detailed soil and climatic data reduce errors in this type of model?In addition,PULSE techniques have not been used to model deciduous species,which are a challenge for ecophysiological models due to their phenology.This study developed a PULSE model for a clonal Populus tomentosa plantation in northern China using detailed edaphic and climatic data.The results showed high precision and low bias in height(m)and basal area(m^(2)·ha^(-1))predictions.While detailed edaphoclimatic data produce highly precise predictions and a good mechanistic understanding,the study suggested that local climatic data could also be employed.The study showed that PULSE modelling in combination with coarse level of edaphic and local climate data resulted in reasonably precise tree growth prediction and minimal bias.
基金supported by the Sichuan Outstanding Young Scientific and Technological Talents Project(No.2021JDJQ0016)Doctoral Initiation Project of China West Normal University(No.22k E043)and Science and Technology Project of Sichuan Province(No.2022NSFSC0388)。
文摘With the rapid development of nuclear energy,the removal of radioactive iodine generated during spent fuel reprocessing has become increasingly important.Based on the unique straw-like structure of populus tomentosa fiber(PTF)and the highly active iodine vapor capture ability of zero-valent silver nanoparticles(PTF@Ag^(0)NP),an Ag^(0)NP composite functional material with highly efficient iodine vapor capture capability was synthesized from biowaste PTF through ultrasonic and hightemperature hydrothermal methods in this study.The iodine capture experiment demonstrated that PTF@Ag^(0)NP exhibits rapid iodine capture efficiency,reaching dynamic equilibrium within 4 h and a maximum capture capacity of 1008.1 mg/g.Density functional theory calculations show that PTF@Ag^(0)NP exhibits extremely high chemical reactivity toward iodine,with a reaction binding energy of-2.88 e V.Additionally,the molecular dynamics of PTF@Ag^(0)NP indicate that there is no atomic displacement at 77?C,indicating the excellent temperature stability of the material at the operating temperature.The capture mechanism suggests that iodine vapor primarily reacts with Ag^(0)NP to form Ag I,and that the hydroxyl groups in PTF can also effectively capture iodine vapor by adsorption induction.In conclusion,PTF@Ag^(0)NP is expected to be an effective candidate adsorbent material for removing radioactive iodine vapor from exhaust gases during spent fuel reprocessing.
基金part of the Liaoning Provincial Department of Education project LJKZ0684supported by the National Natural Science Foundation of China (31800542)
文摘The addition of ectomycorrhizal fungi(ECMF),beneficial rhizosphere microorganisms,to the soil can pro-mote plant growth and resistance.Here,Populus davidi-ana×Populus bolleana tissue culture seedlings were grown for 3 months in soils inoculated with one of the species,then seedlings were assessed for mycorrhizal colonization rate and growth,physiological and root traits.Suillus luteus and Populus involutus each formed ectomycorrhizal associa-tions with the seedlings.Seedling height,ground diameter,biomass,and leaf area were significantly greater after treat-ment with ECMF than in the non-inoculated controls.Treat-ment improved all physiological and root variables assessed(chlorophylls and carotenoids,cellulose,and soluble sugars and proteins;root length,surface area,projected area,mean diameter,volume,number of root tips).Seedlings inocu-lated with S.luteus outperformed those inoculated with P.involutus.
基金funded by the Fundamental Research Funds for the Central Universities(2572019CT02)Heilongjiang Touyan Innovation Team Program(Tree Genetics and Breeding Innovation Team)The Overseas Expertise Introduction Project for Discipline Innovation(B16010).
文摘Populus alba‘Berolinensis’is a fast-growing,high-yielding species with strong biotic and abiotic stress resistance,and widely planted for timber,shelter belts and aesthetic purposes.In this study,molecular development is explored and the important genes regulating xylem forma-tion in P.alba‘Berolinensis’under artificial bending treat-ments was identified.Anatomical investigation indicated that tension wood(TW)was characterized by eccentric growth of xylem and was enriched in cellulose;the degree of ligni-fication was lower than for normal wood(NW)and oppo-site wood(OW).RNA-Seq-based transcriptome analysis was performed using developing xylem from three wood types(TW,OW and NW).A large number of differentially expressed genes(DEGs)were screened and 4889 counted.In GO and KEGG enrichment results,genes involved in plant hormone signal transduction,phenylpropanoid biosynthesis,and cell wall and secondary cell wall biogenesis play major roles in xylem development under artificial bending.Eight expansin(PalEXP)genes were identified from the RNA-seq data;four were differentially expressed during tension wood formation.Phylogenetic analysis indicated that PalEXLB1 belongs to the EXPB subfamily and that the other PalEXPs are members of the EXPA subfamily.A transcriptional regulatory network construction showed 10 transcription factors located in the first and second layers upstream of EXP,including WRKY,ERF and bHLH.RT‒qPCR analy-sis in leaves,stems and roots combined with transcriptome analysis suggests that PalEXPA2,PalEXPA4 and PalEXPA15 play significant regulatory roles in cell wall formation during tension wood development.The candidate genes involved in xylem cell wall development during tension wood formation marks an important step toward identifying the molecular regulatory mechanism of xylem development and wood property improvement in P.alba‘Berolinensis’.
基金funded by the Talents ans its Youth Project of Xinjiang Production and Construction Corps(38000020924,380000358).
文摘Leaves are important‘source’organs that synthesize organic matter,providing carbon sources for plant growth.Here,we used Populus talassica×Populus euphratica,the dominant species in ecological and timber forests,to simulate carbon limitation through artificial 25%,50%,and 75%defoliation treatments and explore the effects on root,stem,and leaf morphology,biomass accumulation,and carbon allocation strategies.At the 60th d after treat-ment,under 25%defoliation treatment,the plant height,specific leaf weight,root surface area and volume,and concentrations of non-structural carbohydrates in stem and root were significantly increased by 9.13%,20.00%,16.60%,31.95%,5.12%,and 9.34%,respectively,relative to the control.There was no significant change in the growth indicators under 50%defoliation treatment,but the concentrations of non-structural carbohydrates in the leaf and stem significantly decreased,showing mostly a negative correlation between them.The opposite was observed in the root.Under 75%defoliation treatment,the plant height,ground diameter,leaf number,single leaf area,root,stem,and total biomass were significantly reduced by 14.15%,10.24%,14.86%,11.31%,11.56%,21.87%,and 16.82%,respectively,relative to the control.The concentrations of non-structural carbohydrates in various organs were significantly reduced,particularly in the consumption of the starch concentrations in the stem and root.These results indicated that carbon allocation strategies can be adjusted to increase the con-centration of non-structural carbohydrates in root and meet plant growth needs under 25%and 50%defoliation.However,75%defoliation significantly limited the distribution of non-structural carbohydrates to roots and stems,reduced carbon storage,and thus inhibited plant growth.Defoliation-induced carbon limitation altered the carbon allocation pattern of P.talassica×P.euphratica,and the relationship between carbon reserves in roots and tree growth recovery after defoliation was greater.This study provides a theoretical basis for the comprehen-sive management of P.talassica×P.euphratica plantations,as well as a reference for the study of plantation car-bon allocation strategies in the desert and semi-desert regions of Xinjiang under carbon-limitation conditions.
文摘As an important ecological tree species in northern China, Populus simonii plays a crucial role in maintaining ecological balance and promoting environmental sustainability. The academic community has conducted a series of in-depth studies on this species, covering key areas such as genomics, survival mechanisms, and genetic breeding. Through the analysis of the genomic structure and function of P. simonii, we have not only revealed the molecular basis for its adaptation to harsh environments but also identified key genes that promote its growth and resistance to pests and diseases. Furthermore, exploring the survival mechanisms of P. simonii has deepened our understanding of its stress resistance traits, including how it effectively copes with abiotic stresses such as drought, salinization, and heavy metal pollution. In genetic breeding, significant progress has been made through the application of modern biotechnology, improving the growth rate and wood quality of P. simonii and enhancing its environmental adaptability and disease resistance. These research findings have not only enriched our knowledge of the biological characteristics of P. simonii but also provided a solid scientific foundation for its application in ecological restoration, forestry production, and environmental management.
基金supported by the National Natural Science Foundation of China(Nos.32271891 and 32171798).
文摘Anoplophora glabripennis is one of the most devastating wood-boring beetles that attacks poplars.However,one poplar species,Populus deltoides,has strong resistance to Anoplophora glabripennis infestation,the underlying defense mechanisms against Anoplophora glabripennis are poorly understood.Secondary metabolites play a crucial role in plants to combat biological stress.Here,based on transcriptome and metabolome,we demonstrated that the mechanisms for responses to mechanical damage and insect infestation were different.The degree of reactions to adult groove production,larval incubation,and larval frass production was not identical.In addition,the potential genes with insect resistance activity were identified.Predominant differentially expressed genes(DEGs)found in the phloem of Populus deltoides include anthocyanidin 3-O-glucosyltransferase5(PdUGT72E),peroxidase 73(PdPod73),peroxidase A2(PdPodA2)and macrophage migration inhibitory factor(PdMIF)responded to stress caused by Anoplophora glabripennis,which further resulted in activation of the plant defense system against insects via changes in regulation of metabolic pathways,such as tyrosine metabolism pathway,phenylpropanoid biosynthesis pathway and flavonoid biosynthesis pathway.Therefore,this work has laid a foundation for further unraveling the mechanisms involved in this interaction.
基金Supported by Natural Science Foundation of China(31160110)Open Project of Key Laboratory of Biological Resource Protection and Utilization of Tarim Basin(BRYB1003)the Principle Fund of Tarim University(TDZKSS201419)~~
文摘This study presented an improved CTAB method for extracting DNA from leaves of Populus euphratica Oliv. and Populus pruinosa Schrenk. based on the conventional CTAB method. The results showed that preventing DNA from browning is a key step to obtain the high-quality DNA during DNA extraction, and under the condition of grinding in the presence of liquid nitrogen, adding such three antioxidants as PVP dry powder, Vc and β-mercaptoethanol could prevent DNA from browning effectively. The total DNA extracted by the improved CTAB method was subjected to PCR detection which proved that it totally satisfied the requirements of subsequent study.
文摘The physical-mechanical properties of Populus x canadensis Moench and Populus x euramericana （Dode） Guinier cv. Gelrica were studied to provide theoret- ical and scientific bases for the directional breeding and efficient use of artificial forests with P. canadensis and P. euramericana Gelrica. The results showed the air-dried density, basic density of P. canadensis were 0.468 g/cm3 and 0.372 g/cm3, respectively; the shrinkage coefficient of radial, tangential and volume were 0.133%, 0.270% and 0.553%, respectively;the modulus of elasticity in static bending, the bending strength and the compressive strength parallel to grain were 9 302.99 MPa, 79.69 MPa and 40.32 Mpa, respectively. As for the P. euramericana Gelrica, the air-dried density, basic densitywere 0.453 and 0.355 g/cm3, respectively; the shrink- age coefficient of radial, tangential and volume were 0.205%, 0.304% and 0.554%, respectively; the modulus of elasticity in static bending, the bending strength and the compressive strength parallel to grain were 9 014.44, 55.87 and 33.09 Mpa respectively. Comprehensive analysis of the indicators showed that the properties of P. canadensis were better than those of P.euramericana Gelrica.
文摘cDNA encoding caffeoyl CoA O-methyltransferase (CCoAOMT) from Chinese white poplar ( Populus tomentosa Carr.) was cloned by RT-PCR and sequenced. Northern analysis displayed that the CCoAOMT was expressed specifically in the developing secondary xylem and its expression was coincident with lignification. The antisense CCoAOMT cDNA was transformed into P. tremula x P. alba mediated by Agrobacterium tumefaciens ( Smith et Townsend) Conn. Transgenic plants were identified with PCR, PCR-Southern and Southern analysis. Lignin content in 5- to 6-month-old transgenic plants was measured. One of the transgenic lines had significant reduction of 17.9% in Klason lignin content as compared with that of untransformed poplar. The results demonstrate that antisense repression of CCoAOMT is an efficient way to reduce lignin content for improving pulping property in engineered trees.
