OBJECTIVE To assess the effects of polyprenols(isolated from Picea abies L.spruce needles)on muscle strength/tone and coordination,and to investigate whether polyprenols may protect atorvastatin-mediated muscle streng...OBJECTIVE To assess the effects of polyprenols(isolated from Picea abies L.spruce needles)on muscle strength/tone and coordination,and to investigate whether polyprenols may protect atorvastatin-mediated muscle strength/tone weakness in female Wistar rats.METHODS Polyprenols at doses of 1,10 and 20mg·kg-1,atorvastatin 80mg·kg-1 or their combination were administered once daily per os for 16 consecutive days in Wistar female rats(n=9-10 per group).Assessment of muscle strength was performed by grip strength test(on day 15)and wire hang test(on day 16).Rotarod test was used to measured locomotor coordination and muscle tone(on day 16).General locomotor activity was evaluated in open field test(on day 15).Assessment of plasma cholesterol level and creatine kinase activity was done on day 17.RESULTS Atorvastatin-treated rats exhibited a marked decrease in grasping strength and hanging time.PP(20mg·kg-1)significantly protected against atorvastatin-induced muscle weakness in grip strength test,and restored it to control values.At all doses,polyprenols prolonged hanging time which was decreased by atorvastatin in wire hang test.Polyprenols per se at 1 and 10mg·kg-1 did not show difference compared to control group animals,while only at 20mg·kg-1 h anging time was prolonged vs.control in wire hang test.No changes between control and tested groups were observed in rotarod and open field tests.Blood cholesterol level was not changed in any of tested groups in female Wistar rats.Polyprenols(20mg·kg-1)significantly(by 25%)increased plasma creatine kinase activity but it was not affected by the combined treatment.CONCLUSION Since polyprenols acted as protectors of atorvastatin-induced muscle weakness,the combination of polyprenols with atorvastatin may be helpful for reducing muscle-related side effects in patients receiving a long-term atorvastatin therapy.展开更多
Ficaprenol-11 (polyprenol) (1), squalene (2), lupeol (3), ?-sitostenone (4), trans-coumaric acid (5), cis-coumaric acid (6) pheophytin-a (7), pheophorbide a methyl ester (8), methyl-132-hydroxy-(132-S)- pheophorbide-b...Ficaprenol-11 (polyprenol) (1), squalene (2), lupeol (3), ?-sitostenone (4), trans-coumaric acid (5), cis-coumaric acid (6) pheophytin-a (7), pheophorbide a methyl ester (8), methyl-132-hydroxy-(132-S)- pheophorbide-b (9) and aristo-phyll-C (10) were isolated from the whole plants of Leucaena leucocephala (Leguminosae). Among them, 1 and 2 was found for the first time from this species. The structure of these compounds were characterized and identified by spectra analyses.展开更多
Brassinosteroids(BRs)are essential plant steroids that regulate various growth and developmental processes(Nolan et al.,2020).Brassinosteroid biosynthesis involves multiple cytochrome P450 enzymes and a single nonCYP4...Brassinosteroids(BRs)are essential plant steroids that regulate various growth and developmental processes(Nolan et al.,2020).Brassinosteroid biosynthesis involves multiple cytochrome P450 enzymes and a single nonCYP450 enzyme DET2(Zebosi et al.,2024),which was named after its dark“de-etiolated”mutant phenotype(Chory et al.,1991).DET2,an Arabidopsis homolog of the mammalian steroid 5α-reductases(SRD5As),catalyzes the reduction of theΔ^(4,5)double bond of 3-oxo-Δ^(4,5)sterols(Li et al.,1996;Fujioka et al.,1997)(Figure S1).展开更多
基金The project supported by Pharma and Chemistry Competence Center of Latvia,Ltd.L-KC-11-0001with the co-financing of the European Regional Development Fund
文摘OBJECTIVE To assess the effects of polyprenols(isolated from Picea abies L.spruce needles)on muscle strength/tone and coordination,and to investigate whether polyprenols may protect atorvastatin-mediated muscle strength/tone weakness in female Wistar rats.METHODS Polyprenols at doses of 1,10 and 20mg·kg-1,atorvastatin 80mg·kg-1 or their combination were administered once daily per os for 16 consecutive days in Wistar female rats(n=9-10 per group).Assessment of muscle strength was performed by grip strength test(on day 15)and wire hang test(on day 16).Rotarod test was used to measured locomotor coordination and muscle tone(on day 16).General locomotor activity was evaluated in open field test(on day 15).Assessment of plasma cholesterol level and creatine kinase activity was done on day 17.RESULTS Atorvastatin-treated rats exhibited a marked decrease in grasping strength and hanging time.PP(20mg·kg-1)significantly protected against atorvastatin-induced muscle weakness in grip strength test,and restored it to control values.At all doses,polyprenols prolonged hanging time which was decreased by atorvastatin in wire hang test.Polyprenols per se at 1 and 10mg·kg-1 did not show difference compared to control group animals,while only at 20mg·kg-1 h anging time was prolonged vs.control in wire hang test.No changes between control and tested groups were observed in rotarod and open field tests.Blood cholesterol level was not changed in any of tested groups in female Wistar rats.Polyprenols(20mg·kg-1)significantly(by 25%)increased plasma creatine kinase activity but it was not affected by the combined treatment.CONCLUSION Since polyprenols acted as protectors of atorvastatin-induced muscle weakness,the combination of polyprenols with atorvastatin may be helpful for reducing muscle-related side effects in patients receiving a long-term atorvastatin therapy.
文摘Ficaprenol-11 (polyprenol) (1), squalene (2), lupeol (3), ?-sitostenone (4), trans-coumaric acid (5), cis-coumaric acid (6) pheophytin-a (7), pheophorbide a methyl ester (8), methyl-132-hydroxy-(132-S)- pheophorbide-b (9) and aristo-phyll-C (10) were isolated from the whole plants of Leucaena leucocephala (Leguminosae). Among them, 1 and 2 was found for the first time from this species. The structure of these compounds were characterized and identified by spectra analyses.
基金partly supported by grants from the National Natural Science Foundation(NSFC31970187 to L.L.and NSFC31870253 to J.L.)funds from the South China Agricultural University+1 种基金Hong Kong Baptist Universitythe Hong Kong Jockey Club Charities Trust。
文摘Brassinosteroids(BRs)are essential plant steroids that regulate various growth and developmental processes(Nolan et al.,2020).Brassinosteroid biosynthesis involves multiple cytochrome P450 enzymes and a single nonCYP450 enzyme DET2(Zebosi et al.,2024),which was named after its dark“de-etiolated”mutant phenotype(Chory et al.,1991).DET2,an Arabidopsis homolog of the mammalian steroid 5α-reductases(SRD5As),catalyzes the reduction of theΔ^(4,5)double bond of 3-oxo-Δ^(4,5)sterols(Li et al.,1996;Fujioka et al.,1997)(Figure S1).
