Hemoglobin A1c(HbA1c),a key biomarker for long-term glucose regulation,is essential for diagnosing and managing diabetes mellitus.However,conventional HbA1c detection methods often suffer from limited sensitivity,narr...Hemoglobin A1c(HbA1c),a key biomarker for long-term glucose regulation,is essential for diagnosing and managing diabetes mellitus.However,conventional HbA1c detection methods often suffer from limited sensitivity,narrow detection ranges,slow response times,and poor long-term stability.In this study,we developed a high-performance amperometric biosensor for the selective detection of Fructosyl Valine(FV),a model compound for HbA1c,by immobilizing Fructosyl Amino Acid Oxidase(FAAO)onto a glassy carbon electrode modified with electrospun polyaniline/polyindole-Mn_(2)O_(3) nanofibers.Operating at an applied potential of 0.27 V versus Ag/AgCl,the biosensor achieved a rapid detection time of 2 s for FV concentrations up to 50µM,with a signal-to-noise ratio of 3.Under optimized conditions(pH 7.0 and 35℃),the biosensor exhibited a wide linear detection range from 0.1 to 3 mM and a high sensitivity of 38.42µA/mM.Importantly,the sensor retained approximately 70% of its initial activity after 193 days of storage at 4℃,demonstrating excellent long-term stability.These results suggest that the FAAO/polyaniline/polyindole-Mn_(2)O_(3) nanocomposite-based biosensor offers a promising platform for sensitive,rapid,and durable detection of HbA1c,providing significant potential for improving diabetes monitoring and management.展开更多
Non-enzymatic electrochemical sensor was developed for estimation of low-level cholesterol.Polyindole/tungsten carbide(PIN/WC)nanocomposite was synthesized and used as an electroactive material to develop low-cost mod...Non-enzymatic electrochemical sensor was developed for estimation of low-level cholesterol.Polyindole/tungsten carbide(PIN/WC)nanocomposite was synthesized and used as an electroactive material to develop low-cost modified stainless steel plate electrode(SSPE).Surface morphology of developed electrode was characterized by scanning electron microscopy.Electrochemical behavior of cholesterol was investigated through electron impedance spectroscopy,potentiodynamic polarization and cyclic voltammetry in 1-M KOH electrolytic solution.The quantification of cholesterol was studied by square wave voltammetry and differential pulse voltammetry.The calibration plots between the cholesterol concentration and peak current were in linear relation with limit of detection of 1.23×10^(−6) mol L^(−1).The overall result reveals that developed PIN/WC/SSPE electrode has excellent performance for trace-level cholesterol estimation and can be further employed for cholesterol monitoring in blood serum samples.展开更多
High quality poly（5-cyanoindole） （P5CI） films were electrosynthesized by direct anodic oxidation of 5-cyanoindole on stainless steel sheet in the mixed electrolytes of boron trifluoride diethyl etherate （BFEE） a...High quality poly（5-cyanoindole） （P5CI） films were electrosynthesized by direct anodic oxidation of 5-cyanoindole on stainless steel sheet in the mixed electrolytes of boron trifluoride diethyl etherate （BFEE） and diethyl ether （EE） （by volume 1：1） ＋ 0,05 mol L^-1 Bu4NBF4. The film formed can be peeled off the electrode into freestanding films, The addition of EE into BFEE can improve the solubility of monomer. P5CI films obtained from this medium showed excellent electrochemical behavior with conductivity of 10^-2 S cm^-1, Structural studies showed that the polymerization of 5-cyanoindole occurred at the 2,3 position. As-formed P5CI films were thoroughly soluble in strong polar organic solvent dimethyl sulfoxide （DMSO） while partly soluble in tetrahydrofuran （THF） or acetone. Fluorescence spectral studies indicated that P5CI was a good blue-ight emitter.展开更多
文摘Hemoglobin A1c(HbA1c),a key biomarker for long-term glucose regulation,is essential for diagnosing and managing diabetes mellitus.However,conventional HbA1c detection methods often suffer from limited sensitivity,narrow detection ranges,slow response times,and poor long-term stability.In this study,we developed a high-performance amperometric biosensor for the selective detection of Fructosyl Valine(FV),a model compound for HbA1c,by immobilizing Fructosyl Amino Acid Oxidase(FAAO)onto a glassy carbon electrode modified with electrospun polyaniline/polyindole-Mn_(2)O_(3) nanofibers.Operating at an applied potential of 0.27 V versus Ag/AgCl,the biosensor achieved a rapid detection time of 2 s for FV concentrations up to 50µM,with a signal-to-noise ratio of 3.Under optimized conditions(pH 7.0 and 35℃),the biosensor exhibited a wide linear detection range from 0.1 to 3 mM and a high sensitivity of 38.42µA/mM.Importantly,the sensor retained approximately 70% of its initial activity after 193 days of storage at 4℃,demonstrating excellent long-term stability.These results suggest that the FAAO/polyaniline/polyindole-Mn_(2)O_(3) nanocomposite-based biosensor offers a promising platform for sensitive,rapid,and durable detection of HbA1c,providing significant potential for improving diabetes monitoring and management.
文摘Non-enzymatic electrochemical sensor was developed for estimation of low-level cholesterol.Polyindole/tungsten carbide(PIN/WC)nanocomposite was synthesized and used as an electroactive material to develop low-cost modified stainless steel plate electrode(SSPE).Surface morphology of developed electrode was characterized by scanning electron microscopy.Electrochemical behavior of cholesterol was investigated through electron impedance spectroscopy,potentiodynamic polarization and cyclic voltammetry in 1-M KOH electrolytic solution.The quantification of cholesterol was studied by square wave voltammetry and differential pulse voltammetry.The calibration plots between the cholesterol concentration and peak current were in linear relation with limit of detection of 1.23×10^(−6) mol L^(−1).The overall result reveals that developed PIN/WC/SSPE electrode has excellent performance for trace-level cholesterol estimation and can be further employed for cholesterol monitoring in blood serum samples.
基金This work was supported by the Jiangxi Research Center for Material Science & Engineering(No.ZX200401008)the Jiangxi Science and Technology Normal University.
文摘High quality poly（5-cyanoindole） （P5CI） films were electrosynthesized by direct anodic oxidation of 5-cyanoindole on stainless steel sheet in the mixed electrolytes of boron trifluoride diethyl etherate （BFEE） and diethyl ether （EE） （by volume 1：1） ＋ 0,05 mol L^-1 Bu4NBF4. The film formed can be peeled off the electrode into freestanding films, The addition of EE into BFEE can improve the solubility of monomer. P5CI films obtained from this medium showed excellent electrochemical behavior with conductivity of 10^-2 S cm^-1, Structural studies showed that the polymerization of 5-cyanoindole occurred at the 2,3 position. As-formed P5CI films were thoroughly soluble in strong polar organic solvent dimethyl sulfoxide （DMSO） while partly soluble in tetrahydrofuran （THF） or acetone. Fluorescence spectral studies indicated that P5CI was a good blue-ight emitter.
