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大豆种子成熟蛋白PM40基因表达方式分析及其启动子预测
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作者 赵艳 刘阔成 +2 位作者 王珏 马晓宇 孙天国 《华北农学报》 CSCD 北大核心 2020年第3期7-11,共5页
研究大豆种子成熟蛋白PM40基因在不同逆境条件下及大豆各组织中的表达情况,并对其上游启动子序列进行生物信息学预测,为揭示PM40基因表达本质及其启动子的功能研究、利用提供理论依据。利用qRT-PCR方法,检测PM40基因在干旱、盐、低温、... 研究大豆种子成熟蛋白PM40基因在不同逆境条件下及大豆各组织中的表达情况,并对其上游启动子序列进行生物信息学预测,为揭示PM40基因表达本质及其启动子的功能研究、利用提供理论依据。利用qRT-PCR方法,检测PM40基因在干旱、盐、低温、脱落酸条件下的表达;比较该基因在大豆根、茎、叶、花、30DAF、60DAF和90DAF中的表达;克隆PM40基因5′端上游启动子序列,并预测其含有的顺式作用元件。结果表明,PM40基因在干旱、低温和脱落酸处理不同时间下,与未处理相比,表达量均下降,在盐处理条件下,只有处理2 h时表达升高,其余处理时间基因表达下降,说明PM40基因受干旱、低温和脱落酸诱导表达下降,在盐诱导2 h时表达升高;PM40基因在大豆种子中的表达相对较高,尤其是60DAF和90DAF的种子中,相对于根的表达量为35.76,239.75倍;以大豆基因组DNA为模板,克隆PM40基因5′端上游启动子序列1 581 bp,生物信息学预测表明,PM40基因启动子序列中包含多种与种子高表达相关的顺式元件。推测大豆PM40基因启动子可能具有种子特异表达特性。 展开更多
关键词 大豆 pm40基因 启动子 生物信息学 QRT-PCR
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The Potential Role of Powdery Mildew-Resistance Gene Pm40 in Chinese Wheat-Breeding Programs in the Post-Pro21 Era 被引量:3
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作者 Shengwen Tang Yuting Hu +1 位作者 Shengfu Zhong Peigao Luo 《Engineering》 2018年第4期500-506,共7页
Powdery mildew, which is caused by Blumeria graminis f. sp. tritici (Bgt), is an important leaf disease that affects wheat yield. Powdery mildew-resistance (Pro) gene Pro21 was first transferred into wheat in the ... Powdery mildew, which is caused by Blumeria graminis f. sp. tritici (Bgt), is an important leaf disease that affects wheat yield. Powdery mildew-resistance (Pro) gene Pro21 was first transferred into wheat in the 1980s, by translocating the Heuchera villosa chromosome arm 6VS to the wheat chromosome arm 6AL (6VS.6AL). Recently, new Bgt isolates that are virulent to Pm21 have been identified in some wheat fields, indicating that wheat breeders should be aware of the risk of deploying Pm21, although pathological details regarding these virulent isolates still remain to be discovered. Pm40 was identified and mapped on the wheat chromosome arm 7BS from several wheat lines developed from the progenies of a wild cross between wheat and Thinopyrum intermedium. Pm40 offers a broad spectrum of resistance to Bgt, which suggests that it is likely to provide potentially durable resistance. Cytological methods did not detect any large alien chromosomal segment in the wheat lines carrying Pm40. Lines with Pm40 and promising agronomical traits have been released by several wheat-breeding programs in the past several years. Therefore, we believe that Pm40 will play a role in powdery mildew-resistance wheat breeding after Pm21 resistance is overcome by Bgt isolates. In addition, both Prn21 and Pm40 were derived from alien species, suggesting that the resistance genes derived from alien species are potentially more durable or effective than those identified from wheat. 展开更多
关键词 WHEAT Powdery mildew Pro21 pm40 Alien species Native resistance
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小麦L699叶片受白粉菌胁迫后蛋白质的差异表达 被引量:3
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作者 梁银萍 常小丽 +5 位作者 张敏 龚国淑 雷雨 祁小波 罗丽雅 胡玉亭 《麦类作物学报》 CAS CSCD 北大核心 2016年第4期460-464,共5页
为从蛋白组学角度认识小麦抗白粉病机制,以含有抗白粉病新基因Pm40的小麦品系L699为材料,采用蛋白质双向电泳和质谱技术(MALDI-TOF-MS)检测小麦叶片接种白粉菌24h后的差异蛋白。结果表明,经PDQuest软件分析,接种白粉菌后小麦叶片中有18... 为从蛋白组学角度认识小麦抗白粉病机制,以含有抗白粉病新基因Pm40的小麦品系L699为材料,采用蛋白质双向电泳和质谱技术(MALDI-TOF-MS)检测小麦叶片接种白粉菌24h后的差异蛋白。结果表明,经PDQuest软件分析,接种白粉菌后小麦叶片中有18个蛋白质斑点表达量上调,38个蛋白质斑点表达量下调。对表达量上调的蛋白质斑点进行质谱分析和数据库检索,共鉴定出15种蛋白质,其中13种参与防御反应、碳水化合物代谢和蛋白质周转。这些差异蛋白与抗病途径、植株生理过程密切相关,在小麦抗白粉病过程中起很重要的作用。 展开更多
关键词 小麦 白粉菌 pm40 蛋白质 双向电泳
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Identification of long-range transport pathways and potential sources of PM2.5 and PM10 in Beijing from 2014 to 2015 被引量:24
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作者 Deping Li Jianguo Liu +7 位作者 Jiaoshi Zhang Huaqiao Gui Peng Du Tongzhu Yu Jie Wang Yihuai Lu Wenqing Liu Yin Cheng 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2017年第6期214-229,共16页
Trajectory clustering, potential source contribution function (PSCF) and concentration-weighted trajectory (CWT) methods were applied to investigate the transport pathways and identify potential sources of PM2.s a... Trajectory clustering, potential source contribution function (PSCF) and concentration-weighted trajectory (CWT) methods were applied to investigate the transport pathways and identify potential sources of PM2.s and PMIo in different seasons from June 2014 to May 2015 in Beijing. The cluster analyses showed that Beijing was affected by trajectories from the south and southeast in summer and autumn. In winter and spring, Beijing was not only affected by the trajectories from the south and southeast, but was also affected by trajectories from the north and northwest. In addition, the analyses of the pressure profile of backward trajectories showed that backward trajectories, which have important influence on Beijing, were mainly distributed above 970 hPa in summer and autumn and below 950 hPa in spring and winter. This indicates that PM2.s and PMIo were strongly affected by the near surface air masses in summer and autumn and by high altitude air masses in winter and spring. Results of PSCF and CV/T analyses showed that the largest potential source areas were identified in spring, followed by winter and autumn, then summer. In addition, potential source regions of PMIo were similar to those of PM2.5. There were a clear seasonal and spatial variation of the potential source areas of Beijing and the airflow in the horizontal and vertical directions. Therefore, more effective regional emission reduction measures in Beijing's surrounding provinces should be implemented to reduce emissions of regional sources in different seasons. 展开更多
关键词 PM2.5 pm40 Cluster analyses PSCFCWT Beijing
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Spatiotemporal variations of PM_(2.5) and PM_(10) concentrations between31 Chinese cities and their relationships with SO_2,NO_2,CO and O_3 被引量:61
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作者 Yangyang Xie Bin Zhao +1 位作者 Lin Zhang Rong Luo 《Particuology》 SCIE EI CAS CSCD 2015年第3期141-149,共9页
The variations of mass concentrations of PM2.5, PMl0, SO2, NO2, CO, and 03 in 31 Chinese provincial capital cities were analyzed based on data from 286 monitoring sites obtained between March 22, 2013 and March 31,201... The variations of mass concentrations of PM2.5, PMl0, SO2, NO2, CO, and 03 in 31 Chinese provincial capital cities were analyzed based on data from 286 monitoring sites obtained between March 22, 2013 and March 31,2014. By comparing the pollutant concentrations over this length of time, the characteristics of the monthly variations of mass concentrations of air pollutants were determined. We used the Pearson correlation coefficient to establish the relationship between PM2.5, PM10, and the gas pollutants. The results revealed significant differences in the concentration levels of air pollutants and in the variations between the different cities. The Pearson correlation coefficients between PMs and NO2 and SO2 were either high or moderate (PM2.s with NO2: r = 0.256-0.688, mean r = 0,498:PM10 with NO2: r = 0.169-0.713, mean r=0.493; PM2.5 with SO2: r=0.232-0.693, mean r=0.449; PM10 with SO2: r=0.131-0.669, mean r = 0.403). The correlation between PMs and CO was diverse (PM2.5: r = 0.156-0.721, mean r = 0.437; PMl0: r= 0.06-0.67, mean r= 0.380). The correlation between PMs and 03 was either weak or uncorrelated (PM2.s: r= -0,35 to 0.089, mean r= -0.164; PM10: r= -0.279 to 0.078, mean r= -0.127), except in Haikou (PM2.5: r=0.500; PM10: r=0,509). 展开更多
关键词 PM2.5pm40 Atmospheric air pollutantIndoor environmentOutdoor environment
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