Perilipin1(PLIN1)is a major phosphorylated protein that specifically coats the surface of neutral lipid droplets(LDs)in adipocytes and plays a crucial role in regulating the accumulation and hydrolysis of triacylglyce...Perilipin1(PLIN1)is a major phosphorylated protein that specifically coats the surface of neutral lipid droplets(LDs)in adipocytes and plays a crucial role in regulating the accumulation and hydrolysis of triacylglycerol(TG).Mammalian studies have shown that Plin1 gene transcription is mainly regulated by peroxisome proliferator-activated receptorgamma(PPARγ),the master regulator of adipogenesis.However,the regulatory mechanism of the chicken Plin1(c Plin1)gene is poorly understood.The present study aimed to investigate whether Plin1 is regulated by PPARγin chickens and identify its exact molecular mechanism.Reporter gene and expression assays showed that PPARγ2,but not PPARγ1,activated(P<0.01)the cPlin1 gene promoter.An electrophoretic mobility shift assay and mutational analysis revealed that PPARγ2 bound to a special site in the cPlin1 gene promoter to enhance its expression.In summary,our results show that PPARγpromotes the expression of the cPlin1 gene and that PPARγ2 is the main regulatory isoform.展开更多
Fat storage disorders including obesity are pandemic human health problems. As a genetically amenable model organism, Caeno- rhabditis elegans has often been used to explore the molecular mechanisms of fat storage reg...Fat storage disorders including obesity are pandemic human health problems. As a genetically amenable model organism, Caeno- rhabditis elegans has often been used to explore the molecular mechanisms of fat storage regulation. Dye staining of fixed animals and stimulated Raman scattering （SRS） microscopy methods have been used successfully to study fat storage, but a genetic screening system that takes full advantage of C. elegans transparency to perform live imaging of fluorescent protein reporters has not yet been reported. Here, we investigated the tissue-specific expression of the GFP fusion of Perilipin 1 （PLIN1）, a Drosophila lipid droplet-associated protein, in C. elegans. Our results indicate that PLINI：：GFP labels lipid droplets and can be used as a fat storage indicator in live worms. Through an RNAi screen, we further identified several previously uncharacterized new fat storage regulators.展开更多
基金supported by the National Natural Science Foundation of China(31201796 and 32072704)the China Agriculture Research System of MOF and MARA(CARS-41)the Natural Science Foundation of Heilongjiang Province,China(LH2020C017)。
文摘Perilipin1(PLIN1)is a major phosphorylated protein that specifically coats the surface of neutral lipid droplets(LDs)in adipocytes and plays a crucial role in regulating the accumulation and hydrolysis of triacylglycerol(TG).Mammalian studies have shown that Plin1 gene transcription is mainly regulated by peroxisome proliferator-activated receptorgamma(PPARγ),the master regulator of adipogenesis.However,the regulatory mechanism of the chicken Plin1(c Plin1)gene is poorly understood.The present study aimed to investigate whether Plin1 is regulated by PPARγin chickens and identify its exact molecular mechanism.Reporter gene and expression assays showed that PPARγ2,but not PPARγ1,activated(P<0.01)the cPlin1 gene promoter.An electrophoretic mobility shift assay and mutational analysis revealed that PPARγ2 bound to a special site in the cPlin1 gene promoter to enhance its expression.In summary,our results show that PPARγpromotes the expression of the cPlin1 gene and that PPARγ2 is the main regulatory isoform.
基金supported by grants from the Ministry of Science and Technology of the People’s Republic of China(No.2013CB530606)the National Natural ScienceFoundation of China(No.31325018)
文摘Fat storage disorders including obesity are pandemic human health problems. As a genetically amenable model organism, Caeno- rhabditis elegans has often been used to explore the molecular mechanisms of fat storage regulation. Dye staining of fixed animals and stimulated Raman scattering （SRS） microscopy methods have been used successfully to study fat storage, but a genetic screening system that takes full advantage of C. elegans transparency to perform live imaging of fluorescent protein reporters has not yet been reported. Here, we investigated the tissue-specific expression of the GFP fusion of Perilipin 1 （PLIN1）, a Drosophila lipid droplet-associated protein, in C. elegans. Our results indicate that PLINI：：GFP labels lipid droplets and can be used as a fat storage indicator in live worms. Through an RNAi screen, we further identified several previously uncharacterized new fat storage regulators.
