A molecular phylogenetic analysis was undertaken to evaluate the present systematic status of commercially familiar Pleuronectidae fishes in the northwestern Pacific Ocean. We sequenced a 529 bp fragment of the mitoch...A molecular phylogenetic analysis was undertaken to evaluate the present systematic status of commercially familiar Pleuronectidae fishes in the northwestern Pacific Ocean. We sequenced a 529 bp fragment of the mitochondrial DNA （mtDNA） COl gene from 34 individuals of these familiar flatfishes from 11 species of nine genera collected in China and Japan. Genetic relationships among haplotypes of specimens from 11 species for COI gene data were reconstructed using the neighbour-joining method and the maximum parsimony （MP） method. The resulting molecular phylogeny of the 11 species, with a few exceptions, supported the existing taxonomy which was based largely on the morphological characteristics. The most noteworthy findings were that the genus Pleuronichthys diverged before deriving of the common ancestor for the other species; the stone flounder, Kareius biocoloratus, should belong to the genus Platichthys with the latin name Platichthys biocoloratus and the genus Verasper was not the extraordinary differ- entiation from the other species in family Pleuronectidae. The resultant phylogenetic trees were also consistent with multiple origins of direct development within family Pleuronectidae. New proof not only for further illuminating the multiple origins of Pleuronectidae but also for confirmation of the evolutionary relationships among groups in the family will be supported.展开更多
The 5’-end of the mitochondrial control region sequences of three flatfishes (Pleuronectiformes: Pleuronectidae) were amplified and sequenced. These sequences were compared with those of other three Pleuronectids spe...The 5’-end of the mitochondrial control region sequences of three flatfishes (Pleuronectiformes: Pleuronectidae) were amplified and sequenced. These sequences were compared with those of other three Pleuronectids species retrieved from GenBank. A phylogenetic tree was constructed based on the partial control region sequences. The results of phyloge- netic analysis are consistent with those of conventional systematics. Compared to previous studies, the structure of the 5’-end of mitochondrial control region was analyzed. The terminal associated sequence motif and its complementary motif were i- dentified at the 5’-end of the sequences. A conserved sequence block, named as CM5’d, was identified in the 5’-end of con- trol region sequences in all Pleuronectids. Another central conserved sequence block, named as CSB-F, was detected in the central conserved blocks.展开更多
基金The National"863"High-Technology Research and Development Project of China under contract No 2006AA09Z418
文摘A molecular phylogenetic analysis was undertaken to evaluate the present systematic status of commercially familiar Pleuronectidae fishes in the northwestern Pacific Ocean. We sequenced a 529 bp fragment of the mitochondrial DNA （mtDNA） COl gene from 34 individuals of these familiar flatfishes from 11 species of nine genera collected in China and Japan. Genetic relationships among haplotypes of specimens from 11 species for COI gene data were reconstructed using the neighbour-joining method and the maximum parsimony （MP） method. The resulting molecular phylogeny of the 11 species, with a few exceptions, supported the existing taxonomy which was based largely on the morphological characteristics. The most noteworthy findings were that the genus Pleuronichthys diverged before deriving of the common ancestor for the other species; the stone flounder, Kareius biocoloratus, should belong to the genus Platichthys with the latin name Platichthys biocoloratus and the genus Verasper was not the extraordinary differ- entiation from the other species in family Pleuronectidae. The resultant phylogenetic trees were also consistent with multiple origins of direct development within family Pleuronectidae. New proof not only for further illuminating the multiple origins of Pleuronectidae but also for confirmation of the evolutionary relationships among groups in the family will be supported.
基金the Shandong Foundation of Sciences(No.Y2000D04) the National Key Basic Research Program from the Ministry of Science and Technology of China(No.G19990437).
文摘The 5’-end of the mitochondrial control region sequences of three flatfishes (Pleuronectiformes: Pleuronectidae) were amplified and sequenced. These sequences were compared with those of other three Pleuronectids species retrieved from GenBank. A phylogenetic tree was constructed based on the partial control region sequences. The results of phyloge- netic analysis are consistent with those of conventional systematics. Compared to previous studies, the structure of the 5’-end of mitochondrial control region was analyzed. The terminal associated sequence motif and its complementary motif were i- dentified at the 5’-end of the sequences. A conserved sequence block, named as CM5’d, was identified in the 5’-end of con- trol region sequences in all Pleuronectids. Another central conserved sequence block, named as CSB-F, was detected in the central conserved blocks.
