Objective To observe the changes of sciatic nerve conduction velocity （SNCV） and interleukin-1α （IL-1α）, phospholipase A2 （PLA2） and prostaglandin E2 （PGE2） in neucleus pulposus tissue in experimental rabbit...Objective To observe the changes of sciatic nerve conduction velocity （SNCV） and interleukin-1α （IL-1α）, phospholipase A2 （PLA2） and prostaglandin E2 （PGE2） in neucleus pulposus tissue in experimental rabbits with lumbar disc herniation （LDH） ; and discuss the mechanism of LDH treated with bloodletting on Wěizhōng （委中 BL40）. Methods 40 normal named control group, LDH model group, BL40 group New Zealand rabbits were randomized into 4 groups, and comparative point group. The experimental LDH model was prepared by the self-made LDH animal pathological modeling device. Bloodletting on BL40 or on comparative point was applied for 7 days. BL-410 physiological signal recorder was used to record SNCV. Enzyme-linked immunosorbent assay （ELISA） and radioimmunology methods were used to determine the contents of IL-1α and PLA2 in neucleus pulposus tissue and PGE2 in serum. Results In BL40 group, SNCV was significantly increased after treatment comparing with that before treatment （P〈0. 01 ）, and the difference in SNCV value before and after treatment was significantly bigger than that in model group and comparative point group （P〈0.05）. Bloodletting on BL40 reduced the contents of IL-1α and PLA2 in neucleus pulposus tissue and serum PGE2 in LDH rabbits, which indicated significant difference or very significant results in the comparison with model and comparative point groups （P〈0.01 or 0. 05）. Conclusion Bloodletting speeded up rapidly SNCV and reduced the contents of inflammatory factors, i.e. IL-1α, PLA2 and serum PGE2 in LDH rabbits, which indicated that alleviation of local inflammatory reaction is probably one of the mechanisms on LDH treated by bloodletting on BL40.展开更多
目的分析血清M型磷脂酶A2受体1(PLA2R1)在诊断、鉴别诊断成人特发性膜性肾病(IMN)的临床价值。方法选择2014年1月—2016年12月接受肾穿刺成功活检明确诊断IMN的患者25例、乙肝相关性肾炎(HBVMN)患者23例、膜性狼疮性肾炎(MLN)患者22例,...目的分析血清M型磷脂酶A2受体1(PLA2R1)在诊断、鉴别诊断成人特发性膜性肾病(IMN)的临床价值。方法选择2014年1月—2016年12月接受肾穿刺成功活检明确诊断IMN的患者25例、乙肝相关性肾炎(HBVMN)患者23例、膜性狼疮性肾炎(MLN)患者22例,均在清晨空腹状态下抽取静脉血样本,检测血清PLA2R1浓度,同时分析24 h尿蛋白定量(24 h Pro)水平,分析IMN患者中PLA2R1表达的差异性。计量资料比较采用单因素方差分析及SNK-q检验,计数资料比较采用χ2检验或非参数检验,P<0.05为差异有统计学意义。结果 MLN患者以及HBV-MN患者经肾穿刺活检、外周静脉血样本检验结果显示均无PLA2R1抗体表达,而IMN患者肾脏内的PLA2R1抗原及抗体的表达率分别为84.00%、72.00%;PLA2R1抗原呈阳性表达者24 h Pro含量高于阴性表达者且血清白蛋白水平低于阴性表达者(均P<0.05)。结论利用PLA2R1对IMN进行诊断及鉴别诊断,能够获得良好的临床效果,而PLA2R1抗原浓度与IMN病情严重程度、临床表现之间关系密切。展开更多
文摘Objective To observe the changes of sciatic nerve conduction velocity （SNCV） and interleukin-1α （IL-1α）, phospholipase A2 （PLA2） and prostaglandin E2 （PGE2） in neucleus pulposus tissue in experimental rabbits with lumbar disc herniation （LDH） ; and discuss the mechanism of LDH treated with bloodletting on Wěizhōng （委中 BL40）. Methods 40 normal named control group, LDH model group, BL40 group New Zealand rabbits were randomized into 4 groups, and comparative point group. The experimental LDH model was prepared by the self-made LDH animal pathological modeling device. Bloodletting on BL40 or on comparative point was applied for 7 days. BL-410 physiological signal recorder was used to record SNCV. Enzyme-linked immunosorbent assay （ELISA） and radioimmunology methods were used to determine the contents of IL-1α and PLA2 in neucleus pulposus tissue and PGE2 in serum. Results In BL40 group, SNCV was significantly increased after treatment comparing with that before treatment （P〈0. 01 ）, and the difference in SNCV value before and after treatment was significantly bigger than that in model group and comparative point group （P〈0.05）. Bloodletting on BL40 reduced the contents of IL-1α and PLA2 in neucleus pulposus tissue and serum PGE2 in LDH rabbits, which indicated significant difference or very significant results in the comparison with model and comparative point groups （P〈0.01 or 0. 05）. Conclusion Bloodletting speeded up rapidly SNCV and reduced the contents of inflammatory factors, i.e. IL-1α, PLA2 and serum PGE2 in LDH rabbits, which indicated that alleviation of local inflammatory reaction is probably one of the mechanisms on LDH treated by bloodletting on BL40.
文摘目的分析血清M型磷脂酶A2受体1(PLA2R1)在诊断、鉴别诊断成人特发性膜性肾病(IMN)的临床价值。方法选择2014年1月—2016年12月接受肾穿刺成功活检明确诊断IMN的患者25例、乙肝相关性肾炎(HBVMN)患者23例、膜性狼疮性肾炎(MLN)患者22例,均在清晨空腹状态下抽取静脉血样本,检测血清PLA2R1浓度,同时分析24 h尿蛋白定量(24 h Pro)水平,分析IMN患者中PLA2R1表达的差异性。计量资料比较采用单因素方差分析及SNK-q检验,计数资料比较采用χ2检验或非参数检验,P<0.05为差异有统计学意义。结果 MLN患者以及HBV-MN患者经肾穿刺活检、外周静脉血样本检验结果显示均无PLA2R1抗体表达,而IMN患者肾脏内的PLA2R1抗原及抗体的表达率分别为84.00%、72.00%;PLA2R1抗原呈阳性表达者24 h Pro含量高于阴性表达者且血清白蛋白水平低于阴性表达者(均P<0.05)。结论利用PLA2R1对IMN进行诊断及鉴别诊断,能够获得良好的临床效果,而PLA2R1抗原浓度与IMN病情严重程度、临床表现之间关系密切。
