冠状病毒内切核糖核酸酶(EndoU)nsp15在通过减少病毒双链RNA(dsRNA)含量以逃避免疫系统识别方面发挥关键作用。然而,关于它与宿主细胞靶标之间的相互作用仍知之甚少。2025年3月17日,中国农业科学院上海兽医研究所廖瑛研究员带领的科研...冠状病毒内切核糖核酸酶(EndoU)nsp15在通过减少病毒双链RNA(dsRNA)含量以逃避免疫系统识别方面发挥关键作用。然而,关于它与宿主细胞靶标之间的相互作用仍知之甚少。2025年3月17日,中国农业科学院上海兽医研究所廖瑛研究员带领的科研团队在国际期刊PLoS Pathogens上发表了题为“Coronavirus endoribonuclease nsp15 suppresses host protein synthesis and evades PKR-eIF2α-mediated translation shutoff to ensure viral protein synthesis”的研究论文,揭示了nsp15 EndoU酶活性在蛋白合成关闭中的独特且保守的作用机制,深化了人们对冠状病毒调控宿主蛋白表达策略的理解。展开更多
AIM： To confirm the predictive factors for interferon （IFN）-α and ribavirin combination therapy for chronic hepatitis patients with hepatitis C virus （HCV） genotype lb. METHODS： HCV RNA from 50 patients infecte...AIM： To confirm the predictive factors for interferon （IFN）-α and ribavirin combination therapy for chronic hepatitis patients with hepatitis C virus （HCV） genotype lb. METHODS： HCV RNA from 50 patients infected with HCV genotype lb was studied by cloning and sequencing of interferon sensitivity determining region （ISDR）, PKR- eIF2α phosphorylation homology domain （PePHD）. Patients were treated with IFN-α and ribavirin for 6 mo and grouped by effectiveness of the therapy. A variety of factors were analyzed. RESULTS： Our data showed that age, HCV RNA titer, and ISDR type could be used as the predictive factors for combined IFN-α and ribavirin efficacy. Characteristically, mutations in PePHD appeared only when the combination therapy was effective. Other factors, such as sex and alanine aminotransferase （ALT） level, were not related to its efficacy. Adjusting for age and HCV RNA titer indicated that the ISDR type was the most potent predictive factor. CONCLUSION： HCV RNA ISDR type is an important factor for predicting efficacy of IFN-α and ribavirin combination therapy in Korean patients.展开更多
文摘冠状病毒内切核糖核酸酶(EndoU)nsp15在通过减少病毒双链RNA(dsRNA)含量以逃避免疫系统识别方面发挥关键作用。然而,关于它与宿主细胞靶标之间的相互作用仍知之甚少。2025年3月17日,中国农业科学院上海兽医研究所廖瑛研究员带领的科研团队在国际期刊PLoS Pathogens上发表了题为“Coronavirus endoribonuclease nsp15 suppresses host protein synthesis and evades PKR-eIF2α-mediated translation shutoff to ensure viral protein synthesis”的研究论文,揭示了nsp15 EndoU酶活性在蛋白合成关闭中的独特且保守的作用机制,深化了人们对冠状病毒调控宿主蛋白表达策略的理解。
基金a Research Grant from Yonsei University,Wonju College of Medicine,YUWCM 2004-03
文摘AIM： To confirm the predictive factors for interferon （IFN）-α and ribavirin combination therapy for chronic hepatitis patients with hepatitis C virus （HCV） genotype lb. METHODS： HCV RNA from 50 patients infected with HCV genotype lb was studied by cloning and sequencing of interferon sensitivity determining region （ISDR）, PKR- eIF2α phosphorylation homology domain （PePHD）. Patients were treated with IFN-α and ribavirin for 6 mo and grouped by effectiveness of the therapy. A variety of factors were analyzed. RESULTS： Our data showed that age, HCV RNA titer, and ISDR type could be used as the predictive factors for combined IFN-α and ribavirin efficacy. Characteristically, mutations in PePHD appeared only when the combination therapy was effective. Other factors, such as sex and alanine aminotransferase （ALT） level, were not related to its efficacy. Adjusting for age and HCV RNA titer indicated that the ISDR type was the most potent predictive factor. CONCLUSION： HCV RNA ISDR type is an important factor for predicting efficacy of IFN-α and ribavirin combination therapy in Korean patients.
