目的研究丙酮酸激酶M1型(PKM1)和丙酮酸激酶M2型(PKM2)在大肠癌临床样本中的表达水平,探讨其与大肠癌发生发展的相关性。方法分别采用Western blot法检测大肠癌临床标本和新鲜大肠癌组织及癌旁组织中PKM1和PKM2蛋白表达水平;分析PKM1、P...目的研究丙酮酸激酶M1型(PKM1)和丙酮酸激酶M2型(PKM2)在大肠癌临床样本中的表达水平,探讨其与大肠癌发生发展的相关性。方法分别采用Western blot法检测大肠癌临床标本和新鲜大肠癌组织及癌旁组织中PKM1和PKM2蛋白表达水平;分析PKM1、PKM2蛋白表达与大肠癌发生发展的相关性。结果 Western blot和免疫组化结果均显示:与癌旁组织相比,癌组织中PKM1的表达水平明显降低(P<0.05),PKM2的表达水平明显增高(P<0.05)。相关分析结果表明,PKM1与大肠癌患者年龄,性别,分期均无相关性,PKM2与病理分期密切相关(P<0.05)。结论大肠癌组织中PKM1呈现低表达,PKM2高表达。且PKM2与大肠癌患者的病理分期成正相关。展开更多
Objective: To observe the effect of total flavonoids of Scutellaria barbata (TF‑SB) on the injury of high glucose induced podocytes (MPC‑5) and the influence of Smad4/PKM2/HIF‑1α pathway. Methods: Firstly, CCK8 was u...Objective: To observe the effect of total flavonoids of Scutellaria barbata (TF‑SB) on the injury of high glucose induced podocytes (MPC‑5) and the influence of Smad4/PKM2/HIF‑1α pathway. Methods: Firstly, CCK8 was used to analyze the safety and efficacy concentration of TF‑SB on MPC‑5 cells. Then, MPC‑5 was then divided into the control group, model group and TF‑SB group. In addition to the control group, model group and TF‑SB group were induced by high glucose to establish MPC‑5 cell injury model. The effects of TF‑SB on ATP, apoptosis and ROS levels of MPC‑5 cells were detected respectively. The contents of IL‑1β, TNF‑α, and MCP‑1 were determined by ELISA, the expression abundance of glycolytic genes (GLU1, PFK1 and HK1) were detected by RT‑PCR. Western blot method was used to detect the expression level of related proteins in Smad4/PKM2/HIF‑1α pathway. Results: Compared with the blank group, ATP content, GLU1, PKF1 and HK1 expression abundance of MPC‑5 cells in the model group decreased significantly, apoptosis, ROS level and IL‑1 β、 TNF‑ α And MCP‑1 significantly increased (P<0.01);Compared with model group, ATP content, GLU1, PKF1 and HK1 expression abundance, apoptosis, ROS level and IL‑1β in TF‑SB group were significantly increased , TNF‑ α The contents of MCP‑1 and MCP‑1 decreased significantly (P<0.01). In addition, compared with the blank group, the model group Smad4 and HIF‑1 α The protein expression and PKM2 expression in nucleus were significantly increased, while PKM2 expression in cytoplasm was significantly decreased (P<0.01);Compared with model group, TF‑SB group Smad4, HIF‑1 α The expression of PKM2 in the nucleus and expression of PKM2 were significantly decreased, while the expression of PKM2 in the cytoplasm was significantly increased (P<0.01). Conclusion: TF‑SB promotes the mitochondrial activity of MPC‑5 cells to induce glycolysis, and then inhibits the secretion of inflammation, which may play a role in treating diabetes nephropathy by inhibiting Smad4/PKM2/HIF‑1α signaling pathway.展开更多
文摘目的研究丙酮酸激酶M1型(PKM1)和丙酮酸激酶M2型(PKM2)在大肠癌临床样本中的表达水平,探讨其与大肠癌发生发展的相关性。方法分别采用Western blot法检测大肠癌临床标本和新鲜大肠癌组织及癌旁组织中PKM1和PKM2蛋白表达水平;分析PKM1、PKM2蛋白表达与大肠癌发生发展的相关性。结果 Western blot和免疫组化结果均显示:与癌旁组织相比,癌组织中PKM1的表达水平明显降低(P<0.05),PKM2的表达水平明显增高(P<0.05)。相关分析结果表明,PKM1与大肠癌患者年龄,性别,分期均无相关性,PKM2与病理分期密切相关(P<0.05)。结论大肠癌组织中PKM1呈现低表达,PKM2高表达。且PKM2与大肠癌患者的病理分期成正相关。
基金Heilongjiang Traditional Chinese Medicine Research Project (No.ZHY19?058)。
文摘Objective: To observe the effect of total flavonoids of Scutellaria barbata (TF‑SB) on the injury of high glucose induced podocytes (MPC‑5) and the influence of Smad4/PKM2/HIF‑1α pathway. Methods: Firstly, CCK8 was used to analyze the safety and efficacy concentration of TF‑SB on MPC‑5 cells. Then, MPC‑5 was then divided into the control group, model group and TF‑SB group. In addition to the control group, model group and TF‑SB group were induced by high glucose to establish MPC‑5 cell injury model. The effects of TF‑SB on ATP, apoptosis and ROS levels of MPC‑5 cells were detected respectively. The contents of IL‑1β, TNF‑α, and MCP‑1 were determined by ELISA, the expression abundance of glycolytic genes (GLU1, PFK1 and HK1) were detected by RT‑PCR. Western blot method was used to detect the expression level of related proteins in Smad4/PKM2/HIF‑1α pathway. Results: Compared with the blank group, ATP content, GLU1, PKF1 and HK1 expression abundance of MPC‑5 cells in the model group decreased significantly, apoptosis, ROS level and IL‑1 β、 TNF‑ α And MCP‑1 significantly increased (P<0.01);Compared with model group, ATP content, GLU1, PKF1 and HK1 expression abundance, apoptosis, ROS level and IL‑1β in TF‑SB group were significantly increased , TNF‑ α The contents of MCP‑1 and MCP‑1 decreased significantly (P<0.01). In addition, compared with the blank group, the model group Smad4 and HIF‑1 α The protein expression and PKM2 expression in nucleus were significantly increased, while PKM2 expression in cytoplasm was significantly decreased (P<0.01);Compared with model group, TF‑SB group Smad4, HIF‑1 α The expression of PKM2 in the nucleus and expression of PKM2 were significantly decreased, while the expression of PKM2 in the cytoplasm was significantly increased (P<0.01). Conclusion: TF‑SB promotes the mitochondrial activity of MPC‑5 cells to induce glycolysis, and then inhibits the secretion of inflammation, which may play a role in treating diabetes nephropathy by inhibiting Smad4/PKM2/HIF‑1α signaling pathway.
