Objective:To investigate the expression of pyruvate kinase M2(PKM2)and RNA binding proteins 1(SERBP1)in colorectal cancer(CRC)and their clinical significance.Methods:A total of 101 cases of colorectal adenocarcinoma t...Objective:To investigate the expression of pyruvate kinase M2(PKM2)and RNA binding proteins 1(SERBP1)in colorectal cancer(CRC)and their clinical significance.Methods:A total of 101 cases of colorectal adenocarcinoma tissues and their corresponding adjacent tissues were collected from our hospital from December 2020 to December 2023.The immunohistochemical Elivision method was used to detect the expression of PKM2 and SERBP1 in CRC and corresponding adjacent tissues.The experimental data were analyzed using statistical software SPSS 27.0.Results:The expression rates of PKM2 and SERBP1 in CRC were higher than those in adjacent tissues.The expression of PKM2 was positively correlated with lymph node metastasis and TNM stage.The expression of SERBP1 was positively correlated with the degree of differentiation,lymph node metastasis,and TNM stage of CRC.Conclusion:PKM2 and SERBP1 may promote the occurrence and tumor progression of CRC,but further experimental research is still needed.展开更多
目的:研究PKM2在非小细胞肺癌组织中的表达及其与放疗敏感性的关系。方法:选取2011年5月至2013年10月四川省人民医院非小细胞肺癌120例患者,术后进行2个月的放射治疗,按照疗效将患者分为4组。RT-PCR检测患者放疗前手术切除的肿瘤组织和...目的:研究PKM2在非小细胞肺癌组织中的表达及其与放疗敏感性的关系。方法:选取2011年5月至2013年10月四川省人民医院非小细胞肺癌120例患者,术后进行2个月的放射治疗,按照疗效将患者分为4组。RT-PCR检测患者放疗前手术切除的肿瘤组织和癌旁组织中PKM2 m RNA表达水平。Western blot和免疫组织化学检测肿瘤组织和癌旁组织中PKM2蛋白表达。RT-PCR和Western blot方法检测不同疗效组PKM2表达水平。结果:非小细胞肺癌放疗2个月后,治疗有效率为49.2%。PKM2在非小细胞肺癌癌组织中表达显著高于相应的癌旁组织。PKM2表达水平与患者放疗疗效呈负相关。结论:PKM2在非小细胞肺癌中表达高于癌旁组织,PKM2低表达患者对放疗敏感性高。展开更多
基金Research Project of Xi’an Health Commission(Project No.:2023yb11)。
文摘Objective:To investigate the expression of pyruvate kinase M2(PKM2)and RNA binding proteins 1(SERBP1)in colorectal cancer(CRC)and their clinical significance.Methods:A total of 101 cases of colorectal adenocarcinoma tissues and their corresponding adjacent tissues were collected from our hospital from December 2020 to December 2023.The immunohistochemical Elivision method was used to detect the expression of PKM2 and SERBP1 in CRC and corresponding adjacent tissues.The experimental data were analyzed using statistical software SPSS 27.0.Results:The expression rates of PKM2 and SERBP1 in CRC were higher than those in adjacent tissues.The expression of PKM2 was positively correlated with lymph node metastasis and TNM stage.The expression of SERBP1 was positively correlated with the degree of differentiation,lymph node metastasis,and TNM stage of CRC.Conclusion:PKM2 and SERBP1 may promote the occurrence and tumor progression of CRC,but further experimental research is still needed.
文摘目的:研究PKM2在非小细胞肺癌组织中的表达及其与放疗敏感性的关系。方法:选取2011年5月至2013年10月四川省人民医院非小细胞肺癌120例患者,术后进行2个月的放射治疗,按照疗效将患者分为4组。RT-PCR检测患者放疗前手术切除的肿瘤组织和癌旁组织中PKM2 m RNA表达水平。Western blot和免疫组织化学检测肿瘤组织和癌旁组织中PKM2蛋白表达。RT-PCR和Western blot方法检测不同疗效组PKM2表达水平。结果:非小细胞肺癌放疗2个月后,治疗有效率为49.2%。PKM2在非小细胞肺癌癌组织中表达显著高于相应的癌旁组织。PKM2表达水平与患者放疗疗效呈负相关。结论:PKM2在非小细胞肺癌中表达高于癌旁组织,PKM2低表达患者对放疗敏感性高。
