Long non-coding RNAs(lncRNAs),which are RNA molecules longer than 200 nucleotides that do not encode proteins,are implicated in a variety of biological processes,including growth and development.Despite research into ...Long non-coding RNAs(lncRNAs),which are RNA molecules longer than 200 nucleotides that do not encode proteins,are implicated in a variety of biological processes,including growth and development.Despite research into the role of lnc RNAs in skeletal muscle development,the regulatory mechanisms governing ovine skeletal muscle development remain unclear.In this study,we analyzed the expression profiles of lnc RNAs in skeletal muscle from 90-day-old embryos(F90),1-month-old lambs(L30),and 3-year-old adult sheep(A3Y) using RNA sequencing.In total,4738 lnc RNAs were identified,including 997 that were differentially expressed.Short-time series expression miner analysis identified eight significant expression profiles and a subset of lnc RNAs potentially involved in muscle development.Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that the predicted target genes of these lnc RNAs were primarily enriched in pathways associated with muscle development,such as the c AMP and Wnt signaling pathways.Notably,the expression of lnc RNA GTL2 was found to decrease during muscle development.Moreover,GTL2 was highly expressed during the differentiation of skeletal muscle satellite cells(SCs) and was shown to modulate ovine myogenesis by affecting the phosphorylation levels of PKA and CREB.Additionally,GTL2 was found to regulate both the proliferation and differentiation of SCs via the PKACREB signaling pathway.Overall,this study provides a valuable resource and offers novel insights into the functional roles and regulatory mechanisms of lnc RNAs in ovine skeletal muscle growth and development.展开更多
基金supported by the National Key R&D Program of China (2 021YFD1 300 901)Natural Science Foundation of China (32 172 701)Modern Wool Sheep Industry System (CARS-39-01)。
文摘Long non-coding RNAs(lncRNAs),which are RNA molecules longer than 200 nucleotides that do not encode proteins,are implicated in a variety of biological processes,including growth and development.Despite research into the role of lnc RNAs in skeletal muscle development,the regulatory mechanisms governing ovine skeletal muscle development remain unclear.In this study,we analyzed the expression profiles of lnc RNAs in skeletal muscle from 90-day-old embryos(F90),1-month-old lambs(L30),and 3-year-old adult sheep(A3Y) using RNA sequencing.In total,4738 lnc RNAs were identified,including 997 that were differentially expressed.Short-time series expression miner analysis identified eight significant expression profiles and a subset of lnc RNAs potentially involved in muscle development.Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that the predicted target genes of these lnc RNAs were primarily enriched in pathways associated with muscle development,such as the c AMP and Wnt signaling pathways.Notably,the expression of lnc RNA GTL2 was found to decrease during muscle development.Moreover,GTL2 was highly expressed during the differentiation of skeletal muscle satellite cells(SCs) and was shown to modulate ovine myogenesis by affecting the phosphorylation levels of PKA and CREB.Additionally,GTL2 was found to regulate both the proliferation and differentiation of SCs via the PKACREB signaling pathway.Overall,this study provides a valuable resource and offers novel insights into the functional roles and regulatory mechanisms of lnc RNAs in ovine skeletal muscle growth and development.
