目的探讨γ干扰素(interferon-gamma,INF-γ)、微小RNA-29a(microRNA-29a,miR-29a)、原癌基因PIM-1(proto-oncogene PIM-1,PIM-1)标志物在结核性胸膜炎合并胸腔积液中的表达水平变化及其与临床特征的关系。方法选取2021年5月—2023年6...目的探讨γ干扰素(interferon-gamma,INF-γ)、微小RNA-29a(microRNA-29a,miR-29a)、原癌基因PIM-1(proto-oncogene PIM-1,PIM-1)标志物在结核性胸膜炎合并胸腔积液中的表达水平变化及其与临床特征的关系。方法选取2021年5月—2023年6月在某医院就诊的107例结核性胸膜炎患者为研究对象,按照诊断结果分为结核性胸膜炎合并胸腔积液组(n=69)和单纯结核性胸膜炎组(n=38)。对比两组INF-γ、miR-29a、PIM-1表达水平差异,Spearman相关系数分析INF-γ、miR-29a、PIM-1表达水平与结核性胸膜炎合并胸腔积液的相关性;受试者操作特征(receiver operator characteristic,ROC)曲线分析INF-γ、miR-29a、PIM-1及其联合检测对结核性胸膜炎合并胸腔积液的预测效能,计量资料数据比较分析采用t检验,计数资料的比较采用χ^(2)检验。结果与单纯结核性胸膜炎组相比,结核性胸膜炎合并积液组的INF-γ(t=-5.440,P<0.001)、miR-29a(t=-3.561,P=0.001)、PIM-1(t=-3.232,P=0.002)均升高;Spearman相关系数分析显示,INF-γ、miR-29a、PIM-1表达水平与结核性胸膜炎合并胸腔积液呈正相关(r=0.443,P<0.001;r=0.332,P<0.001;r=0.291,P=0.002);ROC曲线显示,INF-γ、miR-29a、PIM-1单独及联合检测预测结核性胸膜炎合并胸腔积液的曲线下面积(area under the curve,AUC)分别为0.764、0.701、0.663和0.838,与INF-γ、miR-29a、PIM-1单独检测相比,联合检测对结核性胸膜炎合并胸腔积液具有较高的诊断效能(Z=1.983、2.168、2.859,P=0.047、0.030、0.004)。结论结核性胸膜炎合并胸腔积液患者INF-γ、miR-29a、PIM-1表达水平均表现为特异性的上调,这种联合检测对预测结核性胸膜炎患者是否合并胸腔积液具有良好的预测效能,值得临床推广应用。展开更多
Polymers of intrinsic microporosity(PIMs)have received considerable attention for making high-performance membranes for carbon dioxide separation over the last two decades,owing to their highly permeable porous struct...Polymers of intrinsic microporosity(PIMs)have received considerable attention for making high-performance membranes for carbon dioxide separation over the last two decades,owing to their highly permeable porous structures.However,challenges regarding its relatively low selectivity,physical aging,and plasticisation impede relevant industrial adoptions for gas separation.To address these issues,several strategies including chain modification,post-modification,blending with other polymers,and the addition of fillers,have been developed and explored.PIM-1 is the most investigated PIMs,and hence here we review the stateof-the-arts of the modification strategies of PIM-1 critically and discuss the progress achieved for addressing the aforementioned challenges via meta-analysis.Additionally,the development of PIM-1-based thin film composite membranes is commented as well,shedding light on their potential in industrial gas separation.We hope that the review can be a timely snapshot of the relevant state-of-the-arts of PIMs guiding future design and optimisation of PIMs-based membranes for enhanced performance towards a higher technology readiness level for practical applications.展开更多
目的:观察Pim-1在炎症性肠病发病过程中的动态表达,并观察其与炎症程度的相关性。方法:BALB/c小鼠饮用5%葡聚糖硫酸钠溶液建立急性炎症性肠病模型,分别在第0、1、4、7天取结肠标本,利用real time PCR、免疫组化动态观察Pim-1表达,分析Pi...目的:观察Pim-1在炎症性肠病发病过程中的动态表达,并观察其与炎症程度的相关性。方法:BALB/c小鼠饮用5%葡聚糖硫酸钠溶液建立急性炎症性肠病模型,分别在第0、1、4、7天取结肠标本,利用real time PCR、免疫组化动态观察Pim-1表达,分析Pim-1的表达和疾病活动指数、组织学炎症评分的相关性。结果:饮用5%葡聚糖硫酸钠7天后成功建立小鼠急性炎症性肠病动物模型;Real time PCR、免疫组化结果显示在饮用5%DSS第4、7天后Pim-1表达较正常组及第1天均明显升高,P<0.05,而且Pim-1蛋白主要在淋巴细胞、中性粒细胞等炎症细胞表达;Pearson相关分析表明,结肠组织中Pim-1蛋白与疾病活动指数呈正相关(R=0.868,P<0.01),与组织病理学评分亦呈正相关(R=0.851,P<0.01)。结论:在炎症性肠病起病过程中Pim-1的表达与肠道炎症程度呈正相关,提示Pim-1信号参与肠道炎症反应。展开更多
文摘阿拉贡纳米科学与材料研究所Joaquín Coronas教授领导的团队为了增强混合基质膜(MMMs)中填料与聚合物之间的相容性,对于对二氧化碳具有亲和性的沸石咪唑酯框架材料ZIF-94,通过溶剂辅助配体交换(SALE)方法,用2-十一烷基咪唑盐(umIm)对其进行了部分改性,以改善其与本征微孔聚合物(PIM-1)这一典型聚合物之间的相容性,相关成果发表在《德国应用化学》(Angewandte Chemie International Edition)上。
文摘目的探讨γ干扰素(interferon-gamma,INF-γ)、微小RNA-29a(microRNA-29a,miR-29a)、原癌基因PIM-1(proto-oncogene PIM-1,PIM-1)标志物在结核性胸膜炎合并胸腔积液中的表达水平变化及其与临床特征的关系。方法选取2021年5月—2023年6月在某医院就诊的107例结核性胸膜炎患者为研究对象,按照诊断结果分为结核性胸膜炎合并胸腔积液组(n=69)和单纯结核性胸膜炎组(n=38)。对比两组INF-γ、miR-29a、PIM-1表达水平差异,Spearman相关系数分析INF-γ、miR-29a、PIM-1表达水平与结核性胸膜炎合并胸腔积液的相关性;受试者操作特征(receiver operator characteristic,ROC)曲线分析INF-γ、miR-29a、PIM-1及其联合检测对结核性胸膜炎合并胸腔积液的预测效能,计量资料数据比较分析采用t检验,计数资料的比较采用χ^(2)检验。结果与单纯结核性胸膜炎组相比,结核性胸膜炎合并积液组的INF-γ(t=-5.440,P<0.001)、miR-29a(t=-3.561,P=0.001)、PIM-1(t=-3.232,P=0.002)均升高;Spearman相关系数分析显示,INF-γ、miR-29a、PIM-1表达水平与结核性胸膜炎合并胸腔积液呈正相关(r=0.443,P<0.001;r=0.332,P<0.001;r=0.291,P=0.002);ROC曲线显示,INF-γ、miR-29a、PIM-1单独及联合检测预测结核性胸膜炎合并胸腔积液的曲线下面积(area under the curve,AUC)分别为0.764、0.701、0.663和0.838,与INF-γ、miR-29a、PIM-1单独检测相比,联合检测对结核性胸膜炎合并胸腔积液具有较高的诊断效能(Z=1.983、2.168、2.859,P=0.047、0.030、0.004)。结论结核性胸膜炎合并胸腔积液患者INF-γ、miR-29a、PIM-1表达水平均表现为特异性的上调,这种联合检测对预测结核性胸膜炎患者是否合并胸腔积液具有良好的预测效能,值得临床推广应用。
基金funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No 872102the China Scholarship Council(CSC,file no.202006240076)-University of Manchester joint studentship for supporting the PhD researchthe special innovation project fund from the Institute of Wenzhou,Zhejiang University(No.XMGL-KJZX-202204)。
文摘Polymers of intrinsic microporosity(PIMs)have received considerable attention for making high-performance membranes for carbon dioxide separation over the last two decades,owing to their highly permeable porous structures.However,challenges regarding its relatively low selectivity,physical aging,and plasticisation impede relevant industrial adoptions for gas separation.To address these issues,several strategies including chain modification,post-modification,blending with other polymers,and the addition of fillers,have been developed and explored.PIM-1 is the most investigated PIMs,and hence here we review the stateof-the-arts of the modification strategies of PIM-1 critically and discuss the progress achieved for addressing the aforementioned challenges via meta-analysis.Additionally,the development of PIM-1-based thin film composite membranes is commented as well,shedding light on their potential in industrial gas separation.We hope that the review can be a timely snapshot of the relevant state-of-the-arts of PIMs guiding future design and optimisation of PIMs-based membranes for enhanced performance towards a higher technology readiness level for practical applications.
文摘目的:观察Pim-1在炎症性肠病发病过程中的动态表达,并观察其与炎症程度的相关性。方法:BALB/c小鼠饮用5%葡聚糖硫酸钠溶液建立急性炎症性肠病模型,分别在第0、1、4、7天取结肠标本,利用real time PCR、免疫组化动态观察Pim-1表达,分析Pim-1的表达和疾病活动指数、组织学炎症评分的相关性。结果:饮用5%葡聚糖硫酸钠7天后成功建立小鼠急性炎症性肠病动物模型;Real time PCR、免疫组化结果显示在饮用5%DSS第4、7天后Pim-1表达较正常组及第1天均明显升高,P<0.05,而且Pim-1蛋白主要在淋巴细胞、中性粒细胞等炎症细胞表达;Pearson相关分析表明,结肠组织中Pim-1蛋白与疾病活动指数呈正相关(R=0.868,P<0.01),与组织病理学评分亦呈正相关(R=0.851,P<0.01)。结论:在炎症性肠病起病过程中Pim-1的表达与肠道炎症程度呈正相关,提示Pim-1信号参与肠道炎症反应。
