Appressed and non-appressed lamella membranes of Castor bean leaf chloroplasts were separated by non-ionic detergent Triton-X 100.Appressed membranes showed a high oxygen-evolving activity and low chl a/b ratio. Exami...Appressed and non-appressed lamella membranes of Castor bean leaf chloroplasts were separated by non-ionic detergent Triton-X 100.Appressed membranes showed a high oxygen-evolving activity and low chl a/b ratio. Examining with SDS-PTGE and liquid nitrogen temperature fluorescence measurement showed that they contained only PSII and light-harvesting pigment-protein complexes (LHCP),and there was no detectable amount of PSI. Freeze-fracture electromicroscopic observation confirmed that this part was really an appressed lamella membrane. Through divalent cation Mg^(++), the thylakoid membranes were induced to unstack and restack.With the addition of Mg^(++), the fluorescence intensity was changed instantly. We realized that there existed two processes:One was a rapid process which was accomplished within 30 s. The other was a slow process of which the time duration was about 60 min. This dual effects of Mg^(++) had not been reported before.We had analyzed the change of F685/F730 and discussed the possible rneehanis ms of light energy distribution between photosystems.展开更多
为探究大豆分离蛋白(soybean protein isolate,SPI)对红曲菌产莫纳可林K(monacolin K,MK)及菌丝形态的影响,以丛毛红曲菌(Monascus pilosus)MS-1为实验菌株,在合成培养基中优化SPI影响红曲菌产MK的浓度及pH,并观察其菌丝形态。结果表明,...为探究大豆分离蛋白(soybean protein isolate,SPI)对红曲菌产莫纳可林K(monacolin K,MK)及菌丝形态的影响,以丛毛红曲菌(Monascus pilosus)MS-1为实验菌株,在合成培养基中优化SPI影响红曲菌产MK的浓度及pH,并观察其菌丝形态。结果表明,当SPI添加量为1%且初始pH值为4.0或SPI添加量为3%且初始pH值为2.0时,红曲菌产MK的量可分别达到106.48、105.93 mg/L。另外,SPI能促进红曲菌菌丝生长、菌落直径增加和分生孢子的产生。在SPI作用下,红曲菌发酵前期菌丝体较为饱满但表面不光滑。发酵后期菌丝体变为干瘪、弯曲折叠和断裂,分生孢子凹陷程度更加明显。推测SPI能够促进细胞膜的通透性。该研究结果可为利用SPI偏向性产生MK提供理论依据。展开更多
为探究豆奶中纳米颗粒负载脂溶性天然色素的应用性能,文章利用大豆蛋白纳米颗粒(Soy Protein Nanoparticles,SPN)负载叶黄素(Lutein,LUT)、β-胡萝卜素(β-Carotene,β-CAR)和姜黄素(Curcumin,CUR),分析其在豆奶(soymilk,SM)中水相分散...为探究豆奶中纳米颗粒负载脂溶性天然色素的应用性能,文章利用大豆蛋白纳米颗粒(Soy Protein Nanoparticles,SPN)负载叶黄素(Lutein,LUT)、β-胡萝卜素(β-Carotene,β-CAR)和姜黄素(Curcumin,CUR),分析其在豆奶(soymilk,SM)中水相分散性和贮藏稳定性的强化作用。结果表明,电子鼻对添加负载LUT、β-CAR和CUR纳米颗粒的豆奶中甲基类物质、醇类、醛类和酮类敏感性增强,表明豆奶中挥发性风味物质增加。电子舌表明添加负载LUT、β-CAR和CUR纳米颗粒的豆奶浓郁度、鲜味、苦味、涩味、苦味余味和涩味余味均有所增加,源自水解蛋白中产生鲜味肽和苦味肽。流变特性测试表明添加负载LUT、β-CAR和CUR纳米颗粒后,豆奶呈剪切稀化现象。贮藏稳定性试验表明添加负载LUT、β-CAR和CUR纳米颗粒可提升豆奶贮藏稳定性。展开更多
以抽薹期野生型油菜和黄化突变体叶片为材料,分析了叶片和类囊体膜的光合色素组成、色素与蛋白相对含量;比较了类囊体膜光谱特性(室温吸收光谱、荧光光谱和圆二色谱);用温和电泳、SDS-PAGE分析了类囊体色素蛋白和多肽组成。结果显示:与...以抽薹期野生型油菜和黄化突变体叶片为材料,分析了叶片和类囊体膜的光合色素组成、色素与蛋白相对含量;比较了类囊体膜光谱特性(室温吸收光谱、荧光光谱和圆二色谱);用温和电泳、SDS-PAGE分析了类囊体色素蛋白和多肽组成。结果显示:与野生型相比,突变体叶片的蛋白质含量不变,而Chl a 和Chl b的含量均减少;突变体类囊体膜的Chl a/Chl b 比值较高,Chl/蛋白质比值较低,LHCII色素蛋白复合物的单体和三聚体含量明显减少。突变体的天线系统相对较小、捕光效率较低。展开更多
文摘Appressed and non-appressed lamella membranes of Castor bean leaf chloroplasts were separated by non-ionic detergent Triton-X 100.Appressed membranes showed a high oxygen-evolving activity and low chl a/b ratio. Examining with SDS-PTGE and liquid nitrogen temperature fluorescence measurement showed that they contained only PSII and light-harvesting pigment-protein complexes (LHCP),and there was no detectable amount of PSI. Freeze-fracture electromicroscopic observation confirmed that this part was really an appressed lamella membrane. Through divalent cation Mg^(++), the thylakoid membranes were induced to unstack and restack.With the addition of Mg^(++), the fluorescence intensity was changed instantly. We realized that there existed two processes:One was a rapid process which was accomplished within 30 s. The other was a slow process of which the time duration was about 60 min. This dual effects of Mg^(++) had not been reported before.We had analyzed the change of F685/F730 and discussed the possible rneehanis ms of light energy distribution between photosystems.
文摘为探究豆奶中纳米颗粒负载脂溶性天然色素的应用性能,文章利用大豆蛋白纳米颗粒(Soy Protein Nanoparticles,SPN)负载叶黄素(Lutein,LUT)、β-胡萝卜素(β-Carotene,β-CAR)和姜黄素(Curcumin,CUR),分析其在豆奶(soymilk,SM)中水相分散性和贮藏稳定性的强化作用。结果表明,电子鼻对添加负载LUT、β-CAR和CUR纳米颗粒的豆奶中甲基类物质、醇类、醛类和酮类敏感性增强,表明豆奶中挥发性风味物质增加。电子舌表明添加负载LUT、β-CAR和CUR纳米颗粒的豆奶浓郁度、鲜味、苦味、涩味、苦味余味和涩味余味均有所增加,源自水解蛋白中产生鲜味肽和苦味肽。流变特性测试表明添加负载LUT、β-CAR和CUR纳米颗粒后,豆奶呈剪切稀化现象。贮藏稳定性试验表明添加负载LUT、β-CAR和CUR纳米颗粒可提升豆奶贮藏稳定性。
文摘以抽薹期野生型油菜和黄化突变体叶片为材料,分析了叶片和类囊体膜的光合色素组成、色素与蛋白相对含量;比较了类囊体膜光谱特性(室温吸收光谱、荧光光谱和圆二色谱);用温和电泳、SDS-PAGE分析了类囊体色素蛋白和多肽组成。结果显示:与野生型相比,突变体叶片的蛋白质含量不变,而Chl a 和Chl b的含量均减少;突变体类囊体膜的Chl a/Chl b 比值较高,Chl/蛋白质比值较低,LHCII色素蛋白复合物的单体和三聚体含量明显减少。突变体的天线系统相对较小、捕光效率较低。
