Phytophthora pathogens lead to numerous economically damaging plant diseases worldwide,including potato late blight caused by P.infestans and soybean root rot caused by P.sojae.Our previous work showed that Phytophtho...Phytophthora pathogens lead to numerous economically damaging plant diseases worldwide,including potato late blight caused by P.infestans and soybean root rot caused by P.sojae.Our previous work showed that Phytophthora pathogens may generate abundant phosphatidylinositol 3-phosphate(PI3P)to promote infection via direct association with RxLR effectors.Here,we designed a disease control strategy for metabolizing pathogen-derived PI3P by expressing secreted Arabidopsis thaliana phosphatidylinositol-4-phosphate 5-kinase 1(AtPIP5K1),which can phosphorylate PI3P to PI(3,4)P2.We fused AtPIP5K1 with the soybean PR1a signal peptide(SP-PIP5K1)to enable its secretion into the plant apoplast.Transgenic soybean and potato plants expressing SP-PIP5K1 showed substantially enhanced resistance to various P.sojae and P.infestans isolates,respectively.SP-PIP5K1 significantly reduced PI3P accumulation during P.sojae and soybean interaction.Knockout or inhibition of PI3 kinases(PI3Ks)in P.sojae compromised the resistance mediated by SP-PIP5K1,indicating that SP-PIP5K1 action requires a supply of pathogen-derived PI3P.Furthermore,we revealed that SP-PIP5K1 can interfere with the action of P.sojae mediated by the RxLR effector Avr1k.This novel disease control strategy has the potential to confer durable broad-spectrum Phytophthora resistance in plants through a clear mechanism in which catabolism of PI3P interferes with RxLR effector actions.展开更多
基金supported by the National Natural Science Foundation of China,China(32072507,32272495,and 31721004)the Natural Science Foundation of Jiangsu Province,China(BK20220147).
文摘Phytophthora pathogens lead to numerous economically damaging plant diseases worldwide,including potato late blight caused by P.infestans and soybean root rot caused by P.sojae.Our previous work showed that Phytophthora pathogens may generate abundant phosphatidylinositol 3-phosphate(PI3P)to promote infection via direct association with RxLR effectors.Here,we designed a disease control strategy for metabolizing pathogen-derived PI3P by expressing secreted Arabidopsis thaliana phosphatidylinositol-4-phosphate 5-kinase 1(AtPIP5K1),which can phosphorylate PI3P to PI(3,4)P2.We fused AtPIP5K1 with the soybean PR1a signal peptide(SP-PIP5K1)to enable its secretion into the plant apoplast.Transgenic soybean and potato plants expressing SP-PIP5K1 showed substantially enhanced resistance to various P.sojae and P.infestans isolates,respectively.SP-PIP5K1 significantly reduced PI3P accumulation during P.sojae and soybean interaction.Knockout or inhibition of PI3 kinases(PI3Ks)in P.sojae compromised the resistance mediated by SP-PIP5K1,indicating that SP-PIP5K1 action requires a supply of pathogen-derived PI3P.Furthermore,we revealed that SP-PIP5K1 can interfere with the action of P.sojae mediated by the RxLR effector Avr1k.This novel disease control strategy has the potential to confer durable broad-spectrum Phytophthora resistance in plants through a clear mechanism in which catabolism of PI3P interferes with RxLR effector actions.
文摘目的探讨葛根素对人骨肉瘤细胞株MG63的作用及其机制。方法取对数生长期的MG63细胞分别用不同浓度的葛根素处理(0,25,50,100mmol·L^(-1))。细胞培养48 h后,利用MTT检测MG63细胞增殖水平;流式细胞术检测细胞凋亡水平,RT-PCR检测PI3K、AKT、Bax、Bcl-2、p53和p21 m RNA水平。Western blotting检测PI3K、AKT、Bax、Bcl-2、p53和p21表达水平。结果葛根素浓度依赖性的诱导MG63细胞增殖抑制、凋亡和Bax表达,减少PI3K、AKT、Bcl-2、p53和p21表达。结论葛根素可通过抑制PI3K/AKT/p53信号通路而诱导MG63细胞增殖抑制和凋亡。
