复发性流产是女性常见的妊娠病之一,对女性的身心健康造成一定的伤害和压力,中医称之为“滑胎”。现有中医理论认为,肾气亏虚,冲任不固,胎失所系,是导致滑胎的主要病机。PI3K-AKT信号通路在细胞增殖、存活和代谢调节中发挥重要作用,最...复发性流产是女性常见的妊娠病之一,对女性的身心健康造成一定的伤害和压力,中医称之为“滑胎”。现有中医理论认为,肾气亏虚,冲任不固,胎失所系,是导致滑胎的主要病机。PI3K-AKT信号通路在细胞增殖、存活和代谢调节中发挥重要作用,最近研究发现,PI3K-AKT信号通路可影响胚胎植入过程各种细胞功能的调节,其异常可最终导致复发性流产。本文通过回顾相关研究文献,就PI3K-AKT信号通路在复发性流产发生的作用机制、补肾中药及中药复方在此通路防治复发性流产的机制展开综述,以期为中医药防治复发性流产的研究提供参考。Recurrent abortion is one of the common pregnancy diseases in women, which causes certain harm and pressure to women’s physical and mental health. It is referred to “habitual abortion” in traditional Chinese medicine. According to the existing theory of traditional Chinese medicine, kidney qi deficiency, Chongren deficiency and fetal displacement are the main causes of fetal slip. The PI3K-AKT signaling pathway plays an important role in the regulation of cell proliferation, survival and metabolism. Recent studies have found that the PI3K-AKT signaling pathway can affect the regulation of various cell functions during embryo implantation, and its abnormality may eventually lead to recurrent abortion. By reviewing relevant research literature, this paper reviewed the mechanism of PI3K-AKT signaling pathway in recurrent abortion, and the mechanism of kidney-tonifying Chinese medicine and Chinese medicine compound in preventing recurrent abortion through this pathway, in order to provide reference for the research on the prevention and treatment of recurrent abortion by traditional Chinese medicine.展开更多
目的:探讨骨唾液酸蛋白(bone sialoprotein,BSP)对乳腺癌MDA-MB-231细胞PI3K-AKT信号通路的影响。方法:BSP基因沉默的乳腺癌MDA-MB-231细胞(简称231BO-BSP27)经重组人BSP(recombinant human BSP,rhBSP)和PI3K-AKT抑制剂LY294002处理后,W...目的:探讨骨唾液酸蛋白(bone sialoprotein,BSP)对乳腺癌MDA-MB-231细胞PI3K-AKT信号通路的影响。方法:BSP基因沉默的乳腺癌MDA-MB-231细胞(简称231BO-BSP27)经重组人BSP(recombinant human BSP,rhBSP)和PI3K-AKT抑制剂LY294002处理后,Western blotting检测磷酸化AKT水平的变化,实时定量PCR检测caspase-3、cyclin D1 mRNA表达水平,MTT法检测细胞增殖能力。结果:与BSP基因未沉默的对照组231BO-Scrambled细胞相比,BSP基因沉默的231BO-BSP27细胞BSP蛋白表达明显下调(74.32±2.18)%(P<0.01);AKT磷酸化水平明显下降(33.30±2.61)%(P<0.01),而caspase-3和cyclin D1 mRNA表达分别上升和下降(1.000±0.000 vs 1.733±0.039,1.000±0.000 vs 0.370±0.012;均P<0.01);231BO-BSP27细胞增殖能力显著下降(P<0.05)。外源添加rhBSP蛋白分别上调231BO-Scrambled和231BO-BSP27细胞AKT磷酸化水平(17.86±2.27)%和(33.78±1.51)%(均P<0.01),231BO-BSP27细胞caspase-3 mRNA表达降低(1.000±0.039 vs 0.541±0.091,P<0.01)、cyclin D1 mRNA表达升高(1.000±0.000 vs 2.921±0.032,P<0.01),促进231BO-Scrambled和231BO-BSP27细胞的增殖(均P<0.01)。LY294002则能逆转rhBSP对231BO-Scrambled和231BO-BSP27细胞AKT磷酸化激活作用(P<0.05),使231BO-BSP27细胞caspase-3 mRNA表达升高(P<0.01)、cyclin D1 mRNA表达降低(P<0.01),使该两种细胞增殖能力下降(均P<0.01)。结论:BSP通过PI3K-AKT信号通路调控乳腺癌MDA-MB-231细胞caspase-3和cyclin D1的表达,并影响细胞的增殖。展开更多
文摘复发性流产是女性常见的妊娠病之一,对女性的身心健康造成一定的伤害和压力,中医称之为“滑胎”。现有中医理论认为,肾气亏虚,冲任不固,胎失所系,是导致滑胎的主要病机。PI3K-AKT信号通路在细胞增殖、存活和代谢调节中发挥重要作用,最近研究发现,PI3K-AKT信号通路可影响胚胎植入过程各种细胞功能的调节,其异常可最终导致复发性流产。本文通过回顾相关研究文献,就PI3K-AKT信号通路在复发性流产发生的作用机制、补肾中药及中药复方在此通路防治复发性流产的机制展开综述,以期为中医药防治复发性流产的研究提供参考。Recurrent abortion is one of the common pregnancy diseases in women, which causes certain harm and pressure to women’s physical and mental health. It is referred to “habitual abortion” in traditional Chinese medicine. According to the existing theory of traditional Chinese medicine, kidney qi deficiency, Chongren deficiency and fetal displacement are the main causes of fetal slip. The PI3K-AKT signaling pathway plays an important role in the regulation of cell proliferation, survival and metabolism. Recent studies have found that the PI3K-AKT signaling pathway can affect the regulation of various cell functions during embryo implantation, and its abnormality may eventually lead to recurrent abortion. By reviewing relevant research literature, this paper reviewed the mechanism of PI3K-AKT signaling pathway in recurrent abortion, and the mechanism of kidney-tonifying Chinese medicine and Chinese medicine compound in preventing recurrent abortion through this pathway, in order to provide reference for the research on the prevention and treatment of recurrent abortion by traditional Chinese medicine.
文摘目的:探讨骨唾液酸蛋白(bone sialoprotein,BSP)对乳腺癌MDA-MB-231细胞PI3K-AKT信号通路的影响。方法:BSP基因沉默的乳腺癌MDA-MB-231细胞(简称231BO-BSP27)经重组人BSP(recombinant human BSP,rhBSP)和PI3K-AKT抑制剂LY294002处理后,Western blotting检测磷酸化AKT水平的变化,实时定量PCR检测caspase-3、cyclin D1 mRNA表达水平,MTT法检测细胞增殖能力。结果:与BSP基因未沉默的对照组231BO-Scrambled细胞相比,BSP基因沉默的231BO-BSP27细胞BSP蛋白表达明显下调(74.32±2.18)%(P<0.01);AKT磷酸化水平明显下降(33.30±2.61)%(P<0.01),而caspase-3和cyclin D1 mRNA表达分别上升和下降(1.000±0.000 vs 1.733±0.039,1.000±0.000 vs 0.370±0.012;均P<0.01);231BO-BSP27细胞增殖能力显著下降(P<0.05)。外源添加rhBSP蛋白分别上调231BO-Scrambled和231BO-BSP27细胞AKT磷酸化水平(17.86±2.27)%和(33.78±1.51)%(均P<0.01),231BO-BSP27细胞caspase-3 mRNA表达降低(1.000±0.039 vs 0.541±0.091,P<0.01)、cyclin D1 mRNA表达升高(1.000±0.000 vs 2.921±0.032,P<0.01),促进231BO-Scrambled和231BO-BSP27细胞的增殖(均P<0.01)。LY294002则能逆转rhBSP对231BO-Scrambled和231BO-BSP27细胞AKT磷酸化激活作用(P<0.05),使231BO-BSP27细胞caspase-3 mRNA表达升高(P<0.01)、cyclin D1 mRNA表达降低(P<0.01),使该两种细胞增殖能力下降(均P<0.01)。结论:BSP通过PI3K-AKT信号通路调控乳腺癌MDA-MB-231细胞caspase-3和cyclin D1的表达,并影响细胞的增殖。
