As a new type of semiconductor,Bi-based photocatalysts have attracted widespread attention in the field of environmental pollution control.In this study,a ternary BiPO_(4)/BiOIO_(3)/PI composite was prepared using a f...As a new type of semiconductor,Bi-based photocatalysts have attracted widespread attention in the field of environmental pollution control.In this study,a ternary BiPO_(4)/BiOIO_(3)/PI composite was prepared using a facile hydrothermal process and ultrasonic-assisted deposition method.The Fourier transform infrared spectra(FT-IR),X-ray powder diffraction(XRD),scanning electron microscopy(SEM),and UV-Vis diffused reflection spectra(UV-Vis DRS)were employed to determine the morphology and structure of the as-obtained BiPO_(4)/BiOIO_(3)/PI composite.The photocatalytic performance of the as-prepared BiPO_(4)/BiOIO_(3)/PI was evaluated via the degradation of dyes such as rhodamine B(RhB),methyl orange(MO),and methylene blue(MB)in aqueous solution.It was found that 99.6% of RhB,92.7% of MO,and 87.5%of MB were degraded,within 15 min of simulated solar irradiation over the BiPO_(4)/BiOIO_(3)/PI composite,showing the remarkably high photocatalytic activity of the BiPO_(4)/BiOIO_(3)/PI ternary photocatalyst.The results of trapping experiments displayed that photogenerated holes and superoxide radicals were the main active species in the photocatalytic process.The obvious enhancement of photocatalytic activity could be mainly ascribed to enhancing the separation and migration of photogenerated charge carriers in the BiPO_(4)/BiOIO_(3)/PI composite.展开更多
In order to explore the expression of PI-3K in T lymphocytes of asthmatic rats and the relationship between PI-3K and activation of T lymphocytes, 24 Wistar rats were randomly divided into 4 groups： normal control gr...In order to explore the expression of PI-3K in T lymphocytes of asthmatic rats and the relationship between PI-3K and activation of T lymphocytes, 24 Wistar rats were randomly divided into 4 groups： normal control group, asthmatic one-week group, asthmatic two-week group and asthmatic four-week group. T cells were purified from blood of each rat and the expression of PI-3K was observed by immunocytochemical fluorescence staining, the serniquantitative fluorescence intensity was measured by HPIAS-2000 analytic software, and the expression of IL-4 in supernatants was detected by ELISA. The results showed that the fluorescence intensity of T lymphocytes in asthmatic groups was significantly higher than that in normal control （P〈0.001）, indicating that the expression of PI-3K in T lymphocytes of asthmatic rats was significantly higher than that in those of normal controls, and the difference between acute and chronic stage asthmatic groups was significant （P〈0.05）. The expression levels of IL-4 protein in supernatants of asthmatic T lymphocytes were significantly higher than those in the normal controls （P〈0.05）. There was a significant positive correlation between the expression of PI-3K in T lymphocytes and the IL-4 protein expression in supernatants （r=0.583, P〈0.01）. It was suggested that PI-3K signal pathway may participate in the processes of activation and other cytological effects of asthmatic T lymphocytes, thus may play an important roles in the pathogenesis of asthma.展开更多
Objective: To investigate the mechanism of inflammatory-mediated toll-like receptor 4(TLR4)-p38 mitogen-activated protein kinase(p38 MAPK) pathway in Kupffer cells(KCs) of non-alcoholic steatohepatitis(NASH) rats and ...Objective: To investigate the mechanism of inflammatory-mediated toll-like receptor 4(TLR4)-p38 mitogen-activated protein kinase(p38 MAPK) pathway in Kupffer cells(KCs) of non-alcoholic steatohepatitis(NASH) rats and the intervention effect of soothing Gan(Liver) and invigorating Pi(Spleen) recipes on this pathway. Methods: After 1 week of acclimatization, 120 Sprague-Dawley male rats were randomly divided into 8 groups using a random number table(n=15 per group): normal group, model group, low-dose Chaihu Shugan Powder(柴胡疏肝散, CHSG) group(3.2 g/kg), high-dose CHSG group(9.6 g/kg), low-dose Shenling Baizhu Powder(参苓白术散, SLBZ) group(10 g/kg), high-dose SLBZ(30 g/kg) group, and low-and highdose integrated recipe(L-IR, H-IR) groups. All rats in the model and treatment groups were fed with a high-fat diet(HFD). The treatments were administrated by gastrogavage once daily and lasted for 26 weeks. The liver tissues were detected with hematoxylin-eosin(HE) and oil red O staining. Levels of liver lipids, serum lipids and transaminases were measured. KCs were isolated from the livers of rats to evaluate the mRNA expressions of TLR4 and p38 MAPK by real-time fluorescence quantitative polymerase chain reaction, and proteins expressions of TLR4, p-p38 MAPK and p38 MAPK by Western blot. Levels of inflammatory cytokines including tumor necrosis factor α(TNF-α), interleukin(IL)-1 and IL-6 in KCs were measured by enzyme-linked immunosorbent assay. Results: After 26 weeks of HFD feeding, HE and oil red O staining showed that the NASH model rats successfully reproduced typical pathogenesis and histopathological features. Compared with the normal group, the model group exhibited significant increases in body weight, liver weight, liver index, serum levels of total cholesterol(TC), triglyceride(TG), low-density lipoprotein cholesterol, and aspartate aminotransferase as well as TC and TG levels in liver tissues, and significant decrease in serum level of high-density lipoprotein cholesterol(P<0.05 or P<0.01), while those indices were significantly ameliorated in the H-IR group(P<0.05 or P<0.01). Higher levels of TNF-α, IL-1 and IL-6 in KCs were observed in the model group compared with the normal group(P<0.01). Significant decreases in TNF-α, IL-1 and IL-6 were observed in the H-SLBZ, H-IR and L-IR groups compared with the model group(P<0.05 or P<0.01). The m RNA expressions of TLR4 and p38 MAPK and protein expressions of TLR4, p38 MAPK and p-p38 MAPK in KCs in the model group were significantly higher than the normal group(P<0.01), while those expression levels in the L-IR and H-IR groups were significantly lower than the model group(P<0.05 or P<0.01). Conclusions: Inflammation in KCs might play an important role in the pathogenesis of NASH in rats. The data demonstrated the importance of TLR4-p38 MAPK signaling pathway in KCs for the anti-inflammatory effect of soothing Gan and invigorating Pi recipes.展开更多
基金supported by the Fundamental Research Funds of China West Normal University(19D038,24kx004)the Opening Project of the Key Laboratory of Green Catalysis of Higher Education Institutes of Sichuan(LZJ2101).
文摘As a new type of semiconductor,Bi-based photocatalysts have attracted widespread attention in the field of environmental pollution control.In this study,a ternary BiPO_(4)/BiOIO_(3)/PI composite was prepared using a facile hydrothermal process and ultrasonic-assisted deposition method.The Fourier transform infrared spectra(FT-IR),X-ray powder diffraction(XRD),scanning electron microscopy(SEM),and UV-Vis diffused reflection spectra(UV-Vis DRS)were employed to determine the morphology and structure of the as-obtained BiPO_(4)/BiOIO_(3)/PI composite.The photocatalytic performance of the as-prepared BiPO_(4)/BiOIO_(3)/PI was evaluated via the degradation of dyes such as rhodamine B(RhB),methyl orange(MO),and methylene blue(MB)in aqueous solution.It was found that 99.6% of RhB,92.7% of MO,and 87.5%of MB were degraded,within 15 min of simulated solar irradiation over the BiPO_(4)/BiOIO_(3)/PI composite,showing the remarkably high photocatalytic activity of the BiPO_(4)/BiOIO_(3)/PI ternary photocatalyst.The results of trapping experiments displayed that photogenerated holes and superoxide radicals were the main active species in the photocatalytic process.The obvious enhancement of photocatalytic activity could be mainly ascribed to enhancing the separation and migration of photogenerated charge carriers in the BiPO_(4)/BiOIO_(3)/PI composite.
文摘In order to explore the expression of PI-3K in T lymphocytes of asthmatic rats and the relationship between PI-3K and activation of T lymphocytes, 24 Wistar rats were randomly divided into 4 groups： normal control group, asthmatic one-week group, asthmatic two-week group and asthmatic four-week group. T cells were purified from blood of each rat and the expression of PI-3K was observed by immunocytochemical fluorescence staining, the serniquantitative fluorescence intensity was measured by HPIAS-2000 analytic software, and the expression of IL-4 in supernatants was detected by ELISA. The results showed that the fluorescence intensity of T lymphocytes in asthmatic groups was significantly higher than that in normal control （P〈0.001）, indicating that the expression of PI-3K in T lymphocytes of asthmatic rats was significantly higher than that in those of normal controls, and the difference between acute and chronic stage asthmatic groups was significant （P〈0.05）. The expression levels of IL-4 protein in supernatants of asthmatic T lymphocytes were significantly higher than those in the normal controls （P〈0.05）. There was a significant positive correlation between the expression of PI-3K in T lymphocytes and the IL-4 protein expression in supernatants （r=0.583, P〈0.01）. It was suggested that PI-3K signal pathway may participate in the processes of activation and other cytological effects of asthmatic T lymphocytes, thus may play an important roles in the pathogenesis of asthma.
基金Supported by the National Natural Science Foundation of China(No.30973694)
文摘Objective: To investigate the mechanism of inflammatory-mediated toll-like receptor 4(TLR4)-p38 mitogen-activated protein kinase(p38 MAPK) pathway in Kupffer cells(KCs) of non-alcoholic steatohepatitis(NASH) rats and the intervention effect of soothing Gan(Liver) and invigorating Pi(Spleen) recipes on this pathway. Methods: After 1 week of acclimatization, 120 Sprague-Dawley male rats were randomly divided into 8 groups using a random number table(n=15 per group): normal group, model group, low-dose Chaihu Shugan Powder(柴胡疏肝散, CHSG) group(3.2 g/kg), high-dose CHSG group(9.6 g/kg), low-dose Shenling Baizhu Powder(参苓白术散, SLBZ) group(10 g/kg), high-dose SLBZ(30 g/kg) group, and low-and highdose integrated recipe(L-IR, H-IR) groups. All rats in the model and treatment groups were fed with a high-fat diet(HFD). The treatments were administrated by gastrogavage once daily and lasted for 26 weeks. The liver tissues were detected with hematoxylin-eosin(HE) and oil red O staining. Levels of liver lipids, serum lipids and transaminases were measured. KCs were isolated from the livers of rats to evaluate the mRNA expressions of TLR4 and p38 MAPK by real-time fluorescence quantitative polymerase chain reaction, and proteins expressions of TLR4, p-p38 MAPK and p38 MAPK by Western blot. Levels of inflammatory cytokines including tumor necrosis factor α(TNF-α), interleukin(IL)-1 and IL-6 in KCs were measured by enzyme-linked immunosorbent assay. Results: After 26 weeks of HFD feeding, HE and oil red O staining showed that the NASH model rats successfully reproduced typical pathogenesis and histopathological features. Compared with the normal group, the model group exhibited significant increases in body weight, liver weight, liver index, serum levels of total cholesterol(TC), triglyceride(TG), low-density lipoprotein cholesterol, and aspartate aminotransferase as well as TC and TG levels in liver tissues, and significant decrease in serum level of high-density lipoprotein cholesterol(P<0.05 or P<0.01), while those indices were significantly ameliorated in the H-IR group(P<0.05 or P<0.01). Higher levels of TNF-α, IL-1 and IL-6 in KCs were observed in the model group compared with the normal group(P<0.01). Significant decreases in TNF-α, IL-1 and IL-6 were observed in the H-SLBZ, H-IR and L-IR groups compared with the model group(P<0.05 or P<0.01). The m RNA expressions of TLR4 and p38 MAPK and protein expressions of TLR4, p38 MAPK and p-p38 MAPK in KCs in the model group were significantly higher than the normal group(P<0.01), while those expression levels in the L-IR and H-IR groups were significantly lower than the model group(P<0.05 or P<0.01). Conclusions: Inflammation in KCs might play an important role in the pathogenesis of NASH in rats. The data demonstrated the importance of TLR4-p38 MAPK signaling pathway in KCs for the anti-inflammatory effect of soothing Gan and invigorating Pi recipes.
