Four a-galactosyl phytosphingosine 2,6’-diamide analogs were prepared from 2,6’-diamino a-galactosylphytosphingosine and the aromatic-bearing carboxylic acids. After purification with High Performance Liquid Chromat...Four a-galactosyl phytosphingosine 2,6’-diamide analogs were prepared from 2,6’-diamino a-galactosylphytosphingosine and the aromatic-bearing carboxylic acids. After purification with High Performance Liquid Chromatography, a flowcytometry for the four compounds for stimulation of human Va24+/Vb11+ NKT cell populations was carried out. Additional keto groups on the acyl chains of the 2,6’-diamide compound was associated with the enhanced stimulating effect.展开更多
Ceramides,formed by the dehydration of long-chain fatty acids with phytosphingosine and its derivatives,are widely used in skincare,cosmetics,and pharmaceuticals.Due to the exceedingly low concentration of phytos-phin...Ceramides,formed by the dehydration of long-chain fatty acids with phytosphingosine and its derivatives,are widely used in skincare,cosmetics,and pharmaceuticals.Due to the exceedingly low concentration of phytos-phingosine in plant seeds,relying on the extraction method is highly challenging.Currently,the primary method for obtaining phytosphingosine is the deacetylation of tetraacetyl phytosphingosine(TAPS)derived from fermentation.Wickerhamomyces ciferrii,an unconventional yeast from the pods of Dipteryx odorata,is the only known microorganism capable of naturally secreting TAPS,which is of great industrial value.In recent years,research and applications focused on modifying W.ciferrii for TAPS overproduction have increased rapidly.This review first describes the discovery history,applications,microbial synthesis pathway of TAPS.Research progress in using haploid breeding,mutagenesis breeding,and metabolic engineering to improve TAPS pro-duction is then summarized.In addition,the future prospects of TAPS production using the W.ciferrii platform are discussed in light of the current progress,challenges,and trends in this field.Finally,guidelines for future researches are also emphasized.展开更多
An efficient and practical route to synthesize （2S,3S,4R）-2-azido-3,4-O-isopropyllidene-1,3,4-octadecanetriol from D-galactose in 18% overall yield was described, which required ten steps of reactions and only four ...An efficient and practical route to synthesize （2S,3S,4R）-2-azido-3,4-O-isopropyllidene-1,3,4-octadecanetriol from D-galactose in 18% overall yield was described, which required ten steps of reactions and only four times column chromatography purification.展开更多
文摘Four a-galactosyl phytosphingosine 2,6’-diamide analogs were prepared from 2,6’-diamino a-galactosylphytosphingosine and the aromatic-bearing carboxylic acids. After purification with High Performance Liquid Chromatography, a flowcytometry for the four compounds for stimulation of human Va24+/Vb11+ NKT cell populations was carried out. Additional keto groups on the acyl chains of the 2,6’-diamide compound was associated with the enhanced stimulating effect.
基金supported by the National Natural Science Foundation of China(22178173)the Postdoctoral Fellowship Program of China Postdoctoral Science Foundation(GZC20231119)+3 种基金the China Postdoctoral Science Foundation(2024M751420)the Jiangsu Agricultural Science and Technology Independent Innovation Fund Project(CX(22)3015)the Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture(XTC2204)the Newton Advanced Fellowships(NAF\R1\201187).
文摘Ceramides,formed by the dehydration of long-chain fatty acids with phytosphingosine and its derivatives,are widely used in skincare,cosmetics,and pharmaceuticals.Due to the exceedingly low concentration of phytos-phingosine in plant seeds,relying on the extraction method is highly challenging.Currently,the primary method for obtaining phytosphingosine is the deacetylation of tetraacetyl phytosphingosine(TAPS)derived from fermentation.Wickerhamomyces ciferrii,an unconventional yeast from the pods of Dipteryx odorata,is the only known microorganism capable of naturally secreting TAPS,which is of great industrial value.In recent years,research and applications focused on modifying W.ciferrii for TAPS overproduction have increased rapidly.This review first describes the discovery history,applications,microbial synthesis pathway of TAPS.Research progress in using haploid breeding,mutagenesis breeding,and metabolic engineering to improve TAPS pro-duction is then summarized.In addition,the future prospects of TAPS production using the W.ciferrii platform are discussed in light of the current progress,challenges,and trends in this field.Finally,guidelines for future researches are also emphasized.
文摘An efficient and practical route to synthesize （2S,3S,4R）-2-azido-3,4-O-isopropyllidene-1,3,4-octadecanetriol from D-galactose in 18% overall yield was described, which required ten steps of reactions and only four times column chromatography purification.
