Transition-metal catalyzed cross-coupling is one of the basic strategies for the C-C bond formation.However,it is difficult to achieve satisfactory results when terminal alkynes with electron-withdrawing group such as...Transition-metal catalyzed cross-coupling is one of the basic strategies for the C-C bond formation.However,it is difficult to achieve satisfactory results when terminal alkynes with electron-withdrawing group such as propiolate esters are used.The reason behind this might be the easy polymerization of this type of alkynes in the presence of base.A tetrahydroxydiboron and copper sulfate co-promoted cross-coupling/cyclization of propiolate esters and o-iodobenzoic acid for the facile and efficient construction of phthalides is described.Preliminary mechanism study indicates that tetrahydroxydiboron can inhibit the polymerization of propiolate esters and increase the reaction rate.This method is characterized by high regio-and stereoselectivities,mild reaction conditions,short reaction time,broad substrate scope,and excellent functional group compatibility.展开更多
An efficient and facile method for the synthesis of 3-indolyl-substituted phthalides by Friedel-Crafts alkylation of indoles with 3-hydroxyisobenzofuran-1(3H)-one has been developed. Using only 2 mol-% TsOH·H2O a...An efficient and facile method for the synthesis of 3-indolyl-substituted phthalides by Friedel-Crafts alkylation of indoles with 3-hydroxyisobenzofuran-1(3H)-one has been developed. Using only 2 mol-% TsOH·H2O as the catalyst, various substituted indoles can react smoothly at room temperature to give the corresponding phthalides products in good to excellent yields (up to 96%).展开更多
Phthalides represent an important class of bioactive heterocycle extensively found in pharmaceuticals and natural products,their syntheses have attracted extensively attention.A water-promoted and rhodium-catalyzed[3+...Phthalides represent an important class of bioactive heterocycle extensively found in pharmaceuticals and natural products,their syntheses have attracted extensively attention.A water-promoted and rhodium-catalyzed[3+2]tandem cyclization of aromatic acids and acrylates has been developed in air and neat water free of any additives,which provides an environmentally benign approach for constructing phthalide motifs.Compared with previous literatures,this methodology features quite simple reaction conditions,simple operation and additive-free.Mechanistic studies indicate that apart from the many other well-known oxidations of Rh(I)by external oxidant,hydrogen transfer with the acrylate being a hydrogen acceptor is involved to regenerate the active Rh species,which is different from documented metal-catalyzed phthalides syntheses.The application of this protocol is further demonstrated by the green synthesis of biologically active isoochracinic acid in one step.展开更多
Objective To elucidate potential activities of phthalides and terpenoids from Angelica sinensis by theoretical docking calculation. Methods Eleven components of phthalides and terpenoids were selected as ligand. The c...Objective To elucidate potential activities of phthalides and terpenoids from Angelica sinensis by theoretical docking calculation. Methods Eleven components of phthalides and terpenoids were selected as ligand. The crystalline structures of targets related to common diseases were used as the receptors for calculation. The calculations were conducted with Schr?dinger software package. The grading standard of selectivity was developed according to G-score between ligands and receptors. Results Selective targets of phthalides and terpenoids were related to nevous system diseases, cancer, pain, diabetes, cardiovascular disease, liver cirrhosis, nephrotic syndrome, inflammatory diseases, rheumatoid arthritis, dermatosis, leukemia, microbial inflections, immune diseases, and hypercholesterolemia. In addition to the medical treatments reported in the literature, our research also indicated that these two classes of compounds may be used for tumor, diabetes, rheumatoid arthritis, dermatosis, leukemia, liver cirrhosis, and nephrotic syndrome. According to our research, the effects of phthalides and terpenoids may be not so strong. Conclusion The effects of phthalides and terpenoids on diseases founded through virtual evaluation accord greatly with those reported in experiment and clinic. The combination of computer-aided drug evaluation technique and experiment is definitely an important and fast way to investigate the effects and mechanisms of traditional Chinese medicine.展开更多
A highly diastereoselective and enantioselective Michael addition/desymmetrization reaction of maleimides with prochiral 3-substituted phthalides catalyzed by quinine-derived bifunctional thiourea was realized. A broa...A highly diastereoselective and enantioselective Michael addition/desymmetrization reaction of maleimides with prochiral 3-substituted phthalides catalyzed by quinine-derived bifunctional thiourea was realized. A broad range of the 3,3′-disubstituted phthalides bearing vicinal quaternary-tertiary stereogenic centers were synthesized in moderate to good yields(up to 96%) with high diastereoselectivities(up to >19:1 dr) and enantioselectivities(up to 96:4 er).展开更多
The ruthenium-catalyzed intermolecular cascade cyclization of aromatic acids with aromatic aldehydes, which involves the direct insertion of C–H bond into a polar C=O bond and the successive intramolecular nucleophil...The ruthenium-catalyzed intermolecular cascade cyclization of aromatic acids with aromatic aldehydes, which involves the direct insertion of C–H bond into a polar C=O bond and the successive intramolecular nucleophilic substitution, was developed for the synthesis of 3-substituted phthalides in good to excellent yields. This one-pot procedure characterizes in a short reaction time, the cheaper Ru(II) as a catalyst, readily available acids and aldehydes as starting materials, and water as the only theoretical by-product.These merits make the protocol an efficient and cost-effective route for the synthesis of 3-substituted phthalides.展开更多
The phytochemical progress on Angelica sinensis(Oliv.) Diels over the past decades is summarized. Since 1970 s, 165 chemical constituents, including phthalides, phenylpropanoids, terpenoids and essential oils, aromati...The phytochemical progress on Angelica sinensis(Oliv.) Diels over the past decades is summarized. Since 1970 s, 165 chemical constituents, including phthalides, phenylpropanoids, terpenoids and essential oils, aromatic compounds, alkaloids, alkynes, sterols, fatty acids, and polysaccharides have been isolated or detected from the various parts of the title plant.展开更多
A new dimeric phthalide named Z, Z＇-3.3＇a, 7.7＇a-diligustilide was isolated from the roots of Angelica sinensis. Its structure was determined using spectroscopic methods and X-ray crystallographic diffraction analy...A new dimeric phthalide named Z, Z＇-3.3＇a, 7.7＇a-diligustilide was isolated from the roots of Angelica sinensis. Its structure was determined using spectroscopic methods and X-ray crystallographic diffraction analysis.展开更多
(+)-6-3’a,7-6’-Isowallichilide and(-)-6-3’a,7-6’-isowallichilide,a pair of enantio meric phthalide dimers featuring new 6-3’a,7-6’dimerization sites,were isolated from Ligusticum chuanxiong.The structures were e...(+)-6-3’a,7-6’-Isowallichilide and(-)-6-3’a,7-6’-isowallichilide,a pair of enantio meric phthalide dimers featuring new 6-3’a,7-6’dimerization sites,were isolated from Ligusticum chuanxiong.The structures were elucidated by NMR spectroscopy and X-ray diffraction analysis.Furthermore,the absolute configurations were assigned using experimental and theoretical electronic circular dichroism methods.Their nitric oxide inhibition,antiplatelet aggregation and antioxidant activities were investigated.展开更多
Au/FeOx-TiO2,prepared by deposition-precipitation method,is an efficient and stable catalyst for the liquid phase selective hydrogenation of phthalic anhydride to phthalide under mild reaction conditions.
Some 1-imino-3,3-disubstituted-1,3-dihydro isobenzofuran and 2-(1,1-disubstituted hydroxymethyl) benzamide derivatives have been obtained by the aminolysis of phthalide in the presence of triethylamine/aluminum chlori...Some 1-imino-3,3-disubstituted-1,3-dihydro isobenzofuran and 2-(1,1-disubstituted hydroxymethyl) benzamide derivatives have been obtained by the aminolysis of phthalide in the presence of triethylamine/aluminum chloride. 1-Benzylimino-3,3-disubstituted-1,3-dihydro isobenzofuran can be peroxidized to the corresponding hydroperoxides on exposure to the air for a long period. The structure was characterized by single crystal X-ray diffraction and the possible mechanism was suggested.展开更多
The crystal structure of the title compound（C17H12ClFO5,Mr=350.72） was determined by single-crystal X-ray diffraction.The crystal belongs to monoclinic,space group P21/c,with a=7.693（1）,b=15.767（1）,c=12.986（1）...The crystal structure of the title compound（C17H12ClFO5,Mr=350.72） was determined by single-crystal X-ray diffraction.The crystal belongs to monoclinic,space group P21/c,with a=7.693（1）,b=15.767（1）,c=12.986（1）,β=97.23（1）°,V=1562.6（2）3,Z=4,Dc= 1.491 g/cm3,λ（MoKα）=0.71073,F（000）=720,μ（MoKα）=0.280 mm-1,R=0.0563 and wR=0.1674.A total of 3592 unique reflections were collected,of which 2462 with Ⅰ＆gt;2σ（Ⅰ） were observed.Five-membered lactone rings and hydroxyl-substituted phenyl rings are approximately coplanar with their dihedral angle of 4.1°. The dihedral angle between two different phenyl rings is 71.3°. To our interest, intramolecular O（1）-H（1）…O（4） hydrogen bonds could be found in each molecule. Intramolecular hydrogen bonds and intermolecular van der Waals force contribute to the stability of the structure.展开更多
Objective:To study the effect of butyl phthalide on serum cytokines, soluble apoptosis factors and antioxidant molecules in patients with vascular dementia. Methods:86 patients with vascular dementia admitted in our h...Objective:To study the effect of butyl phthalide on serum cytokines, soluble apoptosis factors and antioxidant molecules in patients with vascular dementia. Methods:86 patients with vascular dementia admitted in our hospital between January 2014 and January 2016 were selected for retrospective study and divided into observation group and control group (n=43) according to double-blind randomized control. Observation group received butyl phthalide capsule (0.2 g/time, 3 times/d) treatment on the basis of conventional treatment, control group received conventional treatment alone, and both lasted for 24 weeks. After 24 weeks of treatment, ELISA was used to determine serum levels of vascular repair factors and soluble apoptosis factors of two groups of patients, and radioimmunoassay was used to determine serum levels of antioxidant molecules. Results:After 24 weeks of treatment, serum vascular repair factors heme oxygenase-1 (HO-1), angiogenin I (AngI) and vascular endothelial growth factor (VEGF) levels of observation group were higher than those of control group, matrix metalloproteinase-9 (MMP-9) level was lower than that of control group, and differences between groups were statistically significant (P<0.05);serum soluble factor-related apoptosis (sFas) and soluble factor-related apoptosis ligand (sFasL) levels were lower than those of control group (P<0.05);serum antioxidant molecules superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), total antioxidant capacity (TAC), catalase (CAT), glutathione S-transferase (GST) and glutathione reductase (GR) levels were higher than those of control group (P<0.05). Conclusions:Butyl phthalide can prompt vascular repair in patients with vascular dementia, and also inhibit apoptosis process and balance the body’s oxidation/antioxidation system function.展开更多
Background:Acute lung injury(ALI)is a high-fatality respiratory disease,and the development of new therapeutic agents is ongoing.Chuanxiong Rhizoma has been prescribed for the therapy of ALI in traditional Chinese med...Background:Acute lung injury(ALI)is a high-fatality respiratory disease,and the development of new therapeutic agents is ongoing.Chuanxiong Rhizoma has been prescribed for the therapy of ALI in traditional Chinese medicine.Objective:The purpose of this article is to explore the effective ingredients,targets,and mechanisms in the treatment of Chuanxiong Rhizoma on ALI.Methods:The active ingredients of Chuanxiong Rhizoma were obtained in the TCMSP,and their potential targets and ALI targets were predicted in TCMSP,Swiss target prediction,and Genecards database.A PPI network was constructed in the string online platform,and KEGG and GO enrichment analysis were conducted in the metascape online platform.Phytochemical investigation of Chuanxiong Rhizoma was performed with chemical separation methods and structural elucidation techniques,and their pharmacological effects were evaluated using LPS-induced mouse ALI model and cell-based assays.Results:Network pharmacology analyses indicated that the treatment of Chuanxiong Rhizoma on ALI was associated with its antiinflammatory effect.The key genes mainly include TNF,STAT3,EGFR,AKT1,BCL2,CASP3,and SRC,and the active compounds are its signature component,phthalides.Phytochemical investigation led to the elucidation of 23 phthalides,which demonstrated an anti-inflammatory effect in vitro and in vivo.Of which,chuanxiongdiolide A,levistilide A,butylphthalide,and senkyunolide A were indeed effective in mitigating ALI in an LPS-induced mouse model.Conclusions:The combined network pharmacological,phytochemical,and pharmacological investigations revealed that the therapeutical effect of Chuanxiong Rhizoma against ALI was associated with inhibition of the inflammatory response by phthalides.展开更多
The ^1 H nuclear magnetic resonance （^1 H NMR） fingerprints of fractionated non-polar extracts （control substance for a plant drug （CSPD） A） from Rhizoma chuanxiong, the rhizomes of Ligusticum chuanxiong Hort., ...The ^1 H nuclear magnetic resonance （^1 H NMR） fingerprints of fractionated non-polar extracts （control substance for a plant drug （CSPD） A） from Rhizoma chuanxiong, the rhizomes of Ligusticum chuanxiong Hort., of seven specimens from different sources were measured on Fourier Transform （FT）-NMR spectrometer and assigned by comparing them with the ^1 H NMR spectra of the isolated pure compounds. The ^1 H NMR fingerprints showed exclusively characteristic resonance signals of the major special constituents of the plant. Although the differences in the relative intensity of the ^1H NMR signals due to a discrepancy in the ratio of the major constituents among these samples could be confirmed by high performance liquid chromatography analysis, the general features of the ^1H NMR fingerprint established for an authentic sample of the rhizomes of L. chuanxiong e. hibited exclusive data from those special compounds and can be used for authenticating L. Chuanxiong species.展开更多
Objective:Chuanxiong Rhizoma(CR,the dried rhizomes of Ligusticum chuanxiong)is a well-known traditional Chinese medicine that promotes qi to activate blood,dispels wind,and relieves pain.To date,more than 118 constitu...Objective:Chuanxiong Rhizoma(CR,the dried rhizomes of Ligusticum chuanxiong)is a well-known traditional Chinese medicine that promotes qi to activate blood,dispels wind,and relieves pain.To date,more than 118 constituents of CR have been isolated and identified.However,the in vivo mechanism of CR decoction is unclear and needs further investigation.In addition,to clarify the effective forms of CR,it is essential to reveal the absorbed constituents and metabolites of CR.Materials and Methods:The absorbed constituents and metabolites in urine and plasma samples of rats orally administered with CR decoction were screened and characterized using a high-performance liquid chromatography with diode array detector and combined with electrospray ionization ion trap time-of-flight multistage mass spectrometry technique.Results:In total,102 compounds,including 11 absorbed constituents(eight phthalides and three phthalic acids)and 91 metabolites(71 phthalide-related and 20 phenolic acid-related),were detected in drug-containing rat urine and plasma samples,among which 33 were new metabolites of either CR or its constituents.Based on the structures of these metabolites,six phthalides(ligustilide,senkyunolide I/H,senkyunolide J/N,and butylidenephthalide)and three phenolic acids(ferulic acid,isoferulic acid,and caffeic acid)were proposed as their precursors.They were also deduced to be the main absorbed constituents of CR decoction,which should have closer relationships with its pharmacological effects than other constituents.Phthalide-related metabolites were formed through the metabolic reactions of hydration,hydroxylation,cysteine conjugation,acetylcysteine conjugation,methanethiol conjugation,mercaptomethanol conjugation,glucuronidation,and sulfation,whereas the phenolic acid-related metabolites were mainly formed by glucuronidation and sulfation.Conclusions:Six phthalides and three phenolic acids were shown to be the main precursors of the metabolites of CR,and 33 compounds were new metabolites of either CR or its constituents.These results are helpful for further understanding of the in vivo mechanism and effective forms of CR.展开更多
文摘Transition-metal catalyzed cross-coupling is one of the basic strategies for the C-C bond formation.However,it is difficult to achieve satisfactory results when terminal alkynes with electron-withdrawing group such as propiolate esters are used.The reason behind this might be the easy polymerization of this type of alkynes in the presence of base.A tetrahydroxydiboron and copper sulfate co-promoted cross-coupling/cyclization of propiolate esters and o-iodobenzoic acid for the facile and efficient construction of phthalides is described.Preliminary mechanism study indicates that tetrahydroxydiboron can inhibit the polymerization of propiolate esters and increase the reaction rate.This method is characterized by high regio-and stereoselectivities,mild reaction conditions,short reaction time,broad substrate scope,and excellent functional group compatibility.
文摘An efficient and facile method for the synthesis of 3-indolyl-substituted phthalides by Friedel-Crafts alkylation of indoles with 3-hydroxyisobenzofuran-1(3H)-one has been developed. Using only 2 mol-% TsOH·H2O as the catalyst, various substituted indoles can react smoothly at room temperature to give the corresponding phthalides products in good to excellent yields (up to 96%).
文摘Phthalides represent an important class of bioactive heterocycle extensively found in pharmaceuticals and natural products,their syntheses have attracted extensively attention.A water-promoted and rhodium-catalyzed[3+2]tandem cyclization of aromatic acids and acrylates has been developed in air and neat water free of any additives,which provides an environmentally benign approach for constructing phthalide motifs.Compared with previous literatures,this methodology features quite simple reaction conditions,simple operation and additive-free.Mechanistic studies indicate that apart from the many other well-known oxidations of Rh(I)by external oxidant,hydrogen transfer with the acrylate being a hydrogen acceptor is involved to regenerate the active Rh species,which is different from documented metal-catalyzed phthalides syntheses.The application of this protocol is further demonstrated by the green synthesis of biologically active isoochracinic acid in one step.
基金Supported by the Ministry of Science and Technology of the P.R.China (No. 2007BAI41B01)
文摘Objective To elucidate potential activities of phthalides and terpenoids from Angelica sinensis by theoretical docking calculation. Methods Eleven components of phthalides and terpenoids were selected as ligand. The crystalline structures of targets related to common diseases were used as the receptors for calculation. The calculations were conducted with Schr?dinger software package. The grading standard of selectivity was developed according to G-score between ligands and receptors. Results Selective targets of phthalides and terpenoids were related to nevous system diseases, cancer, pain, diabetes, cardiovascular disease, liver cirrhosis, nephrotic syndrome, inflammatory diseases, rheumatoid arthritis, dermatosis, leukemia, microbial inflections, immune diseases, and hypercholesterolemia. In addition to the medical treatments reported in the literature, our research also indicated that these two classes of compounds may be used for tumor, diabetes, rheumatoid arthritis, dermatosis, leukemia, liver cirrhosis, and nephrotic syndrome. According to our research, the effects of phthalides and terpenoids may be not so strong. Conclusion The effects of phthalides and terpenoids on diseases founded through virtual evaluation accord greatly with those reported in experiment and clinic. The combination of computer-aided drug evaluation technique and experiment is definitely an important and fast way to investigate the effects and mechanisms of traditional Chinese medicine.
基金supported by the National Natural Science Foundation of China(21390400)
文摘A highly diastereoselective and enantioselective Michael addition/desymmetrization reaction of maleimides with prochiral 3-substituted phthalides catalyzed by quinine-derived bifunctional thiourea was realized. A broad range of the 3,3′-disubstituted phthalides bearing vicinal quaternary-tertiary stereogenic centers were synthesized in moderate to good yields(up to 96%) with high diastereoselectivities(up to >19:1 dr) and enantioselectivities(up to 96:4 er).
基金supported by the National Science Foundation of China (21572122, 21376146)Shaanxi Innovative Team of Key Science and Technology (2013KCT-17)the Fundamental Research Funds for the Central Universities (GK201703019, GK 201601005)
文摘The ruthenium-catalyzed intermolecular cascade cyclization of aromatic acids with aromatic aldehydes, which involves the direct insertion of C–H bond into a polar C=O bond and the successive intramolecular nucleophilic substitution, was developed for the synthesis of 3-substituted phthalides in good to excellent yields. This one-pot procedure characterizes in a short reaction time, the cheaper Ru(II) as a catalyst, readily available acids and aldehydes as starting materials, and water as the only theoretical by-product.These merits make the protocol an efficient and cost-effective route for the synthesis of 3-substituted phthalides.
基金supported by the National Science & Technology Supporting Program for the 12th Five-year Plan(2011BAI05B02)the National Training Program of Innovation and Entrepreneurship for Undergraduates(201210731022)
文摘The phytochemical progress on Angelica sinensis(Oliv.) Diels over the past decades is summarized. Since 1970 s, 165 chemical constituents, including phthalides, phenylpropanoids, terpenoids and essential oils, aromatic compounds, alkaloids, alkynes, sterols, fatty acids, and polysaccharides have been isolated or detected from the various parts of the title plant.
基金Supported by NSFC (20172063) The Major State Basic Research Development Program (GrantNo. G2000077506-A) and The project of Chinese Academy of Sciences.
基金financially supported by the General Fund of National Natural Science Foundation of China(No.30472160).
文摘A new dimeric phthalide named Z, Z＇-3.3＇a, 7.7＇a-diligustilide was isolated from the roots of Angelica sinensis. Its structure was determined using spectroscopic methods and X-ray crystallographic diffraction analysis.
基金supported by the Shanghai Municipal Commission of Health and Family Planning(No.ZY3-CCCX-3-5001)the Science and Technology Commission of Shanghai Municipality(Nos.14401901400,15DZ2292000)+1 种基金the Expenditure Budget Program of Shanghai Municipal Education Commission(No.2016YSN24)Xinglin Young Talent Program of Shanghai University of Traditional Chinese Medicine。
文摘(+)-6-3’a,7-6’-Isowallichilide and(-)-6-3’a,7-6’-isowallichilide,a pair of enantio meric phthalide dimers featuring new 6-3’a,7-6’dimerization sites,were isolated from Ligusticum chuanxiong.The structures were elucidated by NMR spectroscopy and X-ray diffraction analysis.Furthermore,the absolute configurations were assigned using experimental and theoretical electronic circular dichroism methods.Their nitric oxide inhibition,antiplatelet aggregation and antioxidant activities were investigated.
基金support provided by Zhejiang Provincial Natural Science Foundation of China(No.Y405108)the Department of Education of Zhejiang Province of China(No.20051409)
文摘Au/FeOx-TiO2,prepared by deposition-precipitation method,is an efficient and stable catalyst for the liquid phase selective hydrogenation of phthalic anhydride to phthalide under mild reaction conditions.
基金the Postdoctoral Foundation of China for the tlnancial support.
文摘Some 1-imino-3,3-disubstituted-1,3-dihydro isobenzofuran and 2-(1,1-disubstituted hydroxymethyl) benzamide derivatives have been obtained by the aminolysis of phthalide in the presence of triethylamine/aluminum chloride. 1-Benzylimino-3,3-disubstituted-1,3-dihydro isobenzofuran can be peroxidized to the corresponding hydroperoxides on exposure to the air for a long period. The structure was characterized by single crystal X-ray diffraction and the possible mechanism was suggested.
基金supported by the National High-tech R&D Program (863 Program) (2006AA020605)the National Basic Research Program of China (973 Program) (2004CB518908)
文摘The crystal structure of the title compound（C17H12ClFO5,Mr=350.72） was determined by single-crystal X-ray diffraction.The crystal belongs to monoclinic,space group P21/c,with a=7.693（1）,b=15.767（1）,c=12.986（1）,β=97.23（1）°,V=1562.6（2）3,Z=4,Dc= 1.491 g/cm3,λ（MoKα）=0.71073,F（000）=720,μ（MoKα）=0.280 mm-1,R=0.0563 and wR=0.1674.A total of 3592 unique reflections were collected,of which 2462 with Ⅰ＆gt;2σ（Ⅰ） were observed.Five-membered lactone rings and hydroxyl-substituted phenyl rings are approximately coplanar with their dihedral angle of 4.1°. The dihedral angle between two different phenyl rings is 71.3°. To our interest, intramolecular O（1）-H（1）…O（4） hydrogen bonds could be found in each molecule. Intramolecular hydrogen bonds and intermolecular van der Waals force contribute to the stability of the structure.
文摘Objective:To study the effect of butyl phthalide on serum cytokines, soluble apoptosis factors and antioxidant molecules in patients with vascular dementia. Methods:86 patients with vascular dementia admitted in our hospital between January 2014 and January 2016 were selected for retrospective study and divided into observation group and control group (n=43) according to double-blind randomized control. Observation group received butyl phthalide capsule (0.2 g/time, 3 times/d) treatment on the basis of conventional treatment, control group received conventional treatment alone, and both lasted for 24 weeks. After 24 weeks of treatment, ELISA was used to determine serum levels of vascular repair factors and soluble apoptosis factors of two groups of patients, and radioimmunoassay was used to determine serum levels of antioxidant molecules. Results:After 24 weeks of treatment, serum vascular repair factors heme oxygenase-1 (HO-1), angiogenin I (AngI) and vascular endothelial growth factor (VEGF) levels of observation group were higher than those of control group, matrix metalloproteinase-9 (MMP-9) level was lower than that of control group, and differences between groups were statistically significant (P<0.05);serum soluble factor-related apoptosis (sFas) and soluble factor-related apoptosis ligand (sFasL) levels were lower than those of control group (P<0.05);serum antioxidant molecules superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), total antioxidant capacity (TAC), catalase (CAT), glutathione S-transferase (GST) and glutathione reductase (GR) levels were higher than those of control group (P<0.05). Conclusions:Butyl phthalide can prompt vascular repair in patients with vascular dementia, and also inhibit apoptosis process and balance the body’s oxidation/antioxidation system function.
基金supported by NNSF of China(81673558 and 81874341)Shandong Provincial Natural Science Foundation(ZR2023LZY003)+1 种基金Multidisciplinary Research and Innovation Program of Shandong University(No.2020QNQT007)Outstanding Young Scholars Program of Shandong University.
文摘Background:Acute lung injury(ALI)is a high-fatality respiratory disease,and the development of new therapeutic agents is ongoing.Chuanxiong Rhizoma has been prescribed for the therapy of ALI in traditional Chinese medicine.Objective:The purpose of this article is to explore the effective ingredients,targets,and mechanisms in the treatment of Chuanxiong Rhizoma on ALI.Methods:The active ingredients of Chuanxiong Rhizoma were obtained in the TCMSP,and their potential targets and ALI targets were predicted in TCMSP,Swiss target prediction,and Genecards database.A PPI network was constructed in the string online platform,and KEGG and GO enrichment analysis were conducted in the metascape online platform.Phytochemical investigation of Chuanxiong Rhizoma was performed with chemical separation methods and structural elucidation techniques,and their pharmacological effects were evaluated using LPS-induced mouse ALI model and cell-based assays.Results:Network pharmacology analyses indicated that the treatment of Chuanxiong Rhizoma on ALI was associated with its antiinflammatory effect.The key genes mainly include TNF,STAT3,EGFR,AKT1,BCL2,CASP3,and SRC,and the active compounds are its signature component,phthalides.Phytochemical investigation led to the elucidation of 23 phthalides,which demonstrated an anti-inflammatory effect in vitro and in vivo.Of which,chuanxiongdiolide A,levistilide A,butylphthalide,and senkyunolide A were indeed effective in mitigating ALI in an LPS-induced mouse model.Conclusions:The combined network pharmacological,phytochemical,and pharmacological investigations revealed that the therapeutical effect of Chuanxiong Rhizoma against ALI was associated with inhibition of the inflammatory response by phthalides.
基金Supported by the National Natural Science Foundation of China (30630073)the Science Foundation of the State Administration of Traditional ChineseMedicine, China (02-03ZP09)
文摘The ^1 H nuclear magnetic resonance （^1 H NMR） fingerprints of fractionated non-polar extracts （control substance for a plant drug （CSPD） A） from Rhizoma chuanxiong, the rhizomes of Ligusticum chuanxiong Hort., of seven specimens from different sources were measured on Fourier Transform （FT）-NMR spectrometer and assigned by comparing them with the ^1 H NMR spectra of the isolated pure compounds. The ^1 H NMR fingerprints showed exclusively characteristic resonance signals of the major special constituents of the plant. Although the differences in the relative intensity of the ^1H NMR signals due to a discrepancy in the ratio of the major constituents among these samples could be confirmed by high performance liquid chromatography analysis, the general features of the ^1H NMR fingerprint established for an authentic sample of the rhizomes of L. chuanxiong e. hibited exclusive data from those special compounds and can be used for authenticating L. Chuanxiong species.
基金financially supported by the National Science and Technology Major Project for Major New Drugs Innovation and Development of China(No.2009ZX09502-006,No.2009ZX09301-010,No.2019ZX09201004-001-023)the National Natural Science Foundation of China(Grant No.30830120,81473321)。
文摘Objective:Chuanxiong Rhizoma(CR,the dried rhizomes of Ligusticum chuanxiong)is a well-known traditional Chinese medicine that promotes qi to activate blood,dispels wind,and relieves pain.To date,more than 118 constituents of CR have been isolated and identified.However,the in vivo mechanism of CR decoction is unclear and needs further investigation.In addition,to clarify the effective forms of CR,it is essential to reveal the absorbed constituents and metabolites of CR.Materials and Methods:The absorbed constituents and metabolites in urine and plasma samples of rats orally administered with CR decoction were screened and characterized using a high-performance liquid chromatography with diode array detector and combined with electrospray ionization ion trap time-of-flight multistage mass spectrometry technique.Results:In total,102 compounds,including 11 absorbed constituents(eight phthalides and three phthalic acids)and 91 metabolites(71 phthalide-related and 20 phenolic acid-related),were detected in drug-containing rat urine and plasma samples,among which 33 were new metabolites of either CR or its constituents.Based on the structures of these metabolites,six phthalides(ligustilide,senkyunolide I/H,senkyunolide J/N,and butylidenephthalide)and three phenolic acids(ferulic acid,isoferulic acid,and caffeic acid)were proposed as their precursors.They were also deduced to be the main absorbed constituents of CR decoction,which should have closer relationships with its pharmacological effects than other constituents.Phthalide-related metabolites were formed through the metabolic reactions of hydration,hydroxylation,cysteine conjugation,acetylcysteine conjugation,methanethiol conjugation,mercaptomethanol conjugation,glucuronidation,and sulfation,whereas the phenolic acid-related metabolites were mainly formed by glucuronidation and sulfation.Conclusions:Six phthalides and three phenolic acids were shown to be the main precursors of the metabolites of CR,and 33 compounds were new metabolites of either CR or its constituents.These results are helpful for further understanding of the in vivo mechanism and effective forms of CR.
