当前个人健康记录(PHR:Personal Health Records)存在着存储不安全和难以共享的问题。为提高PHR的安全性和共享性,提出一种基于超级帐本(Hyperledger)和手指静脉(FV:Finger Vein)特征身份验证的安全共享模型。PHR提供方在录入医疗记录时...当前个人健康记录(PHR:Personal Health Records)存在着存储不安全和难以共享的问题。为提高PHR的安全性和共享性,提出一种基于超级帐本(Hyperledger)和手指静脉(FV:Finger Vein)特征身份验证的安全共享模型。PHR提供方在录入医疗记录时将FV特征与临床医生的私钥签名结合,生成PHR索引。然后将PHR的索引存储在Hyperledger Fabric中,将真正的隐私数据存储在Filecoin中,减少了存储成本和链上存储压力。同时,设计了PHR访问控制合约防止恶意节点攻击。实验结果表明,该模型在性能和存储上有显著的优势。展开更多
Plants are often simultaneously stressed by both UV radiation and phosphorus(P)deficiency in agricultural ecosystems.Coordinated responses and adaptations to these stressors are critical for plant growth,development,a...Plants are often simultaneously stressed by both UV radiation and phosphorus(P)deficiency in agricultural ecosystems.Coordinated responses and adaptations to these stressors are critical for plant growth,development,and survival.However,the underlying molecular response and adaptation mechanisms in plants are not fully understood.Here,we show that plants use a reciprocal antagonistic strategy in response to UV radiation and P deficiency.UV radiation inhibits P-starvation response processes and disrupts phosphate(Pi)homeostasis by suppressing the function of PHOSPHATE STARVATION RESPONSE PROTEINS(PHRs),the Pi central regulators.Conversely,P availability modulates plant UV tolerance and the expression of UV radiation response genes in a PHR-dependent manner.Therefore,reducing the P supply or increasing PHR activities can improve tolerance to UV stress in rice.Moreover,this antagonistic interaction is conserved across various plant species.Our meta-analysis showed that the increase in global UV radiation over the last 40 years may have reduced crop P-utilization efficiency worldwide.Our findings provide insights for optimizing P fertilizer management and breeding smart crops that are resilient to fluctuations in UV radiation and soil P levels.展开更多
Anthocyanin accumulation is acknowledged as a phenotypic indicator of phosphate(Pi)starvation.However,negative regulators of this process and their molecular mechanisms remain largely unexplored.In this study,we demon...Anthocyanin accumulation is acknowledged as a phenotypic indicator of phosphate(Pi)starvation.However,negative regulators of this process and their molecular mechanisms remain largely unexplored.In this study,we demonstrate that WRKY33 acts as a negative regulator of phosphorus-status-dependent anthocyanin biosynthesis.WRKY33 regulates the expression of the gene encoding dihydroflavonol 4-reductase(DFR),a rate-limiting enzyme in anthocyanin production,both directly and indirectly.WRKY33 binds directly to the DFR promoter to repress its expression and also interferes with the MBW complex through interacting with PAP1 to indirectly influence DFR transcriptional activation.Under�Pi conditions,PHR1 interacts with WRKY33,and the protein level of WRKY33 decreases;the repression of DFR expression by WRKY33 is thus attenuated,leading to anthocyanin accumulation in Arabidopsis.Further genetic and biochemical assays suggest that PHR1 is also involved in regulating factors that affect WRKY33 protein turnover.Taken together,ourfindings reveal that Pi starvation represses WRKY33,a repressor of anthocyanin biosynthesis,tofinely tune anthocyanin biosynthesis.This‘‘double-negative logic’’regulation of phosphorus-status-dependent anthocyanin biosynthesis is required for the mainte-nance of plant metabolic homeostasis during acclimation to Pi starvation.展开更多
PHR1(PHOSPHATE STARVATION RESPONSE1)plays key roles in the inorganic phosphate(Pi)starvation response and in Pi deficiency-induced anthocyanin biosynthesis in plants. However, the post-translational regulation of PHR1...PHR1(PHOSPHATE STARVATION RESPONSE1)plays key roles in the inorganic phosphate(Pi)starvation response and in Pi deficiency-induced anthocyanin biosynthesis in plants. However, the post-translational regulation of PHR1 is unclear,and the molecular basis of PHR1-mediated anthocyanin biosynthesis remains elusive. In this study, we determined that MdPHR1 was essential for Pi deficiency-induced anthocyanin accumulation in apple(Malus × domestica). MdPHR1 interacted with MdWRKY75, a positive regulator of anthocyanin biosynthesis, to enhance the MdWRKY75-activated transcription of MdMYB1,leading to anthocyanin accumulation. In addition,the E3 ubiquitin ligase SEVEN IN ABSENTIA1(MdSINA1) negatively regulated MdPHR1-promoted anthocyanin biosynthesis via the ubiquitination-mediated degradation of MdPHR1.Moreover, the protein kinase apple BRASSINOSTEROID INSENSITIVE2(MdBIN2) phosphorylated MdPHR1 and positively regulated MdPHR1-mediated anthocyanin accumulation by attenuating the MdSINA1-mediated ubiquitination degradation of MdPHR1. Taken together,these findings not only demonstrate the regulatory role of MdPHR1 in Pi starvation induced anthocyanin accumulation, but also provide an insight into the post-translational regulation of PHR1.展开更多
文摘当前个人健康记录(PHR:Personal Health Records)存在着存储不安全和难以共享的问题。为提高PHR的安全性和共享性,提出一种基于超级帐本(Hyperledger)和手指静脉(FV:Finger Vein)特征身份验证的安全共享模型。PHR提供方在录入医疗记录时将FV特征与临床医生的私钥签名结合,生成PHR索引。然后将PHR的索引存储在Hyperledger Fabric中,将真正的隐私数据存储在Filecoin中,减少了存储成本和链上存储压力。同时,设计了PHR访问控制合约防止恶意节点攻击。实验结果表明,该模型在性能和存储上有显著的优势。
基金supported by the National Natural Science Foundation of China(32222078 and 32272810)the Fundamental Research Funds for Central Non-profit Scientific Institutionsupported by the Agricultural Science and Technology Innovation Program(ASTIP).
文摘Plants are often simultaneously stressed by both UV radiation and phosphorus(P)deficiency in agricultural ecosystems.Coordinated responses and adaptations to these stressors are critical for plant growth,development,and survival.However,the underlying molecular response and adaptation mechanisms in plants are not fully understood.Here,we show that plants use a reciprocal antagonistic strategy in response to UV radiation and P deficiency.UV radiation inhibits P-starvation response processes and disrupts phosphate(Pi)homeostasis by suppressing the function of PHOSPHATE STARVATION RESPONSE PROTEINS(PHRs),the Pi central regulators.Conversely,P availability modulates plant UV tolerance and the expression of UV radiation response genes in a PHR-dependent manner.Therefore,reducing the P supply or increasing PHR activities can improve tolerance to UV stress in rice.Moreover,this antagonistic interaction is conserved across various plant species.Our meta-analysis showed that the increase in global UV radiation over the last 40 years may have reduced crop P-utilization efficiency worldwide.Our findings provide insights for optimizing P fertilizer management and breeding smart crops that are resilient to fluctuations in UV radiation and soil P levels.
基金funded by the Zhejiang Provincial Natural Science Foundation of China under grant no.LR22C020003the National Key Research and Development Program of China (2022YFD2200603)+2 种基金the National Natural Science Foundation of China under grant nos.32000234 and 32172593the China Postdoctoral Science Foundation (2022M712831)funding from the State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products.
文摘Anthocyanin accumulation is acknowledged as a phenotypic indicator of phosphate(Pi)starvation.However,negative regulators of this process and their molecular mechanisms remain largely unexplored.In this study,we demonstrate that WRKY33 acts as a negative regulator of phosphorus-status-dependent anthocyanin biosynthesis.WRKY33 regulates the expression of the gene encoding dihydroflavonol 4-reductase(DFR),a rate-limiting enzyme in anthocyanin production,both directly and indirectly.WRKY33 binds directly to the DFR promoter to repress its expression and also interferes with the MBW complex through interacting with PAP1 to indirectly influence DFR transcriptional activation.Under�Pi conditions,PHR1 interacts with WRKY33,and the protein level of WRKY33 decreases;the repression of DFR expression by WRKY33 is thus attenuated,leading to anthocyanin accumulation in Arabidopsis.Further genetic and biochemical assays suggest that PHR1 is also involved in regulating factors that affect WRKY33 protein turnover.Taken together,ourfindings reveal that Pi starvation represses WRKY33,a repressor of anthocyanin biosynthesis,tofinely tune anthocyanin biosynthesis.This‘‘double-negative logic’’regulation of phosphorus-status-dependent anthocyanin biosynthesis is required for the mainte-nance of plant metabolic homeostasis during acclimation to Pi starvation.
基金financially supported by grants from the Natural Science Foundation of Shandong Province(ZR2022YQ24)the Development Plan of the Youth Innovation Team of the Higher Education Institutions in Shandong Province (2022KJ326)Wuhan Botanical Garden Scientific Research Support Project (E3559901)。
文摘PHR1(PHOSPHATE STARVATION RESPONSE1)plays key roles in the inorganic phosphate(Pi)starvation response and in Pi deficiency-induced anthocyanin biosynthesis in plants. However, the post-translational regulation of PHR1 is unclear,and the molecular basis of PHR1-mediated anthocyanin biosynthesis remains elusive. In this study, we determined that MdPHR1 was essential for Pi deficiency-induced anthocyanin accumulation in apple(Malus × domestica). MdPHR1 interacted with MdWRKY75, a positive regulator of anthocyanin biosynthesis, to enhance the MdWRKY75-activated transcription of MdMYB1,leading to anthocyanin accumulation. In addition,the E3 ubiquitin ligase SEVEN IN ABSENTIA1(MdSINA1) negatively regulated MdPHR1-promoted anthocyanin biosynthesis via the ubiquitination-mediated degradation of MdPHR1.Moreover, the protein kinase apple BRASSINOSTEROID INSENSITIVE2(MdBIN2) phosphorylated MdPHR1 and positively regulated MdPHR1-mediated anthocyanin accumulation by attenuating the MdSINA1-mediated ubiquitination degradation of MdPHR1. Taken together,these findings not only demonstrate the regulatory role of MdPHR1 in Pi starvation induced anthocyanin accumulation, but also provide an insight into the post-translational regulation of PHR1.
