Therapy-induced modulation of the tumor microenvironment(TME)to overcome the immunosuppressive TME is considered to be a chance for cancer treatment.Herein,we prepared near-infrared absorbing aza-BODIPY PhEt-azaBDP wi...Therapy-induced modulation of the tumor microenvironment(TME)to overcome the immunosuppressive TME is considered to be a chance for cancer treatment.Herein,we prepared near-infrared absorbing aza-BODIPY PhEt-azaBDP with 1-phenylethyl group at 1,7-sites,a type I photodynamic-photothermal therapy(PDT-PTT)agent.Self-assembly PhEt-azaBDP nanoparticles(NPs)can provide combined phototherapeutic effects under light irradiation and simultaneously induce inflammatory TME,by monitoring tumorassociated macrophages(TAMs)repolarization.Utilizing cluster of differentiation 86(CD86)and CD163 as the M1-type marker and M2-type marker respectively,PhEt-azaBDP NPs resulted in the increasement of the expression of CD86 and the decreasement of the expression of CD163 in TAMs under near-infrared(NIR)light irradiation,promoting TAMs to switch from M2-phenotype to M1-phenotype.Inflammatory cytokines,interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α),could be the key cytokine involved in the phototherapy-induced TME reprogramming.PhEt-azaBDP NPs could be a potential theranostic scaffold for the simultaneous induction and detection of TME reprogramming triggered by phototherapy.展开更多
Rational design of multifunctional nanoplatforms capable of combining therapeutic effects with real-time monitoring of drug distribution and tumor status is emerging as a promising approach in cancer nanomedicine.Here...Rational design of multifunctional nanoplatforms capable of combining therapeutic effects with real-time monitoring of drug distribution and tumor status is emerging as a promising approach in cancer nanomedicine.Here,we introduce pyropheophorbide a-bisaminoquinoline conjugate lipid nanoparticles(PPBC LNPs)as a bimodal system for image-guided phototherapy in bladder cancer treatment.PPBC LNPs not only demonstrate both powerful photodynamic and photothermal effects upon light activation,but also exhibit potent autophagy blockage,effectively inducing bladder cancer cell death.Furthermore,PPBC LNPs possess remarkable photoacoustic(PA)and fluorescence(FL)imaging capabilities,enabling imaging with high-resolution,deep tissue penetration and high sensitivity for tracking drug biodistribution and phototherapy efficacy.Specifically,PA imaging confirms the efficient accumulation of PPBC LNPs within tumor and predicts therapeutic outcomes of photodynamic therapy,while FL imaging confirms their prolonged retention at the tumor site for up to 6 days.PPBC LNPs significantly suppress bladder tumor growth,with several tumors completely ablated following just two doses of the nanoparticles and laser treatment.Additionally,PPBC LNPs were formulated with lipid-based excipients and assembled using microfluidic technology to enhance biocompatibility,stability,and scalability,showing potential for clinical translation.This versatile nanoparticle represents a promising candidate for further development in bladder cancer therapy.展开更多
The hippocampus is the brain structure that is responsible for the formation of learning memories.Sleep disorders leading to cognitive impairment are strongly associated with the hippocampus.Phototherapy offers a new ...The hippocampus is the brain structure that is responsible for the formation of learning memories.Sleep disorders leading to cognitive impairment are strongly associated with the hippocampus.Phototherapy offers a new physical therapy for the treatment of sleep disorders,with the advantages of being noninvasive and having few side effects.However,the mechanism by which phototherapy improves cognitive impairment caused by sleep disorders remains unclear.In this study,we used phototherapy combined with optogenetic technology to investigate the effect of noninvasive phototherapy on cognitive functions in sleep-deprived mice.Our results suggest that phototherapy might improve cognitive functions in sleep-deprived mice by modulating the hippocampus.Our study expands the research progress on noninvasive phototherapy for the treatment of sleep disorders.展开更多
Designing and synthesizing nanomedicines with multi-modal tumor therapeutic capabilities is the key to cancer treatment.Herein,we prepared MICG nanoparticles(NPs)by assembling glucose oxidase(GOx)and indocyanine green...Designing and synthesizing nanomedicines with multi-modal tumor therapeutic capabilities is the key to cancer treatment.Herein,we prepared MICG nanoparticles(NPs)by assembling glucose oxidase(GOx)and indocyanine green(ICG)with manganese carbonate(MnCO_(3))NPs for starvation therapy cascaded chemodynamic therapy,enhanced phototherapy and immune activation.In MICG NPs,the GOx consumes intratumoral glucose resulting in starvation therapy,and simultaneously produces H_(2)O_(2)and decreases p H in tumor.The intensified acidic tumor environment promotes the decomposition of MnCO_(3)NPs to release Mn^(2+).The Mn^(2+)further catalyzes H_(2)O_(2)to generate hydroxyl radical for chemodynamic therapy.While ICG can generate singlet oxygen(^(1)O_(2))and heat to kill cancer cells through phototherapy mechanism.The hydroxyl radical and ^(1)O_(2) will further accelerate the oxidative stress,intensify immunogenic cell death,induce dendritic cell maturation,and thus activate systemic immunity.This work provides a new therapeutic platform for combining therapy of tumor.展开更多
Photodynamic therapy(PDT)has garnered significant attention as a promising approach to cancer therapy,harnessing the combined benefits of localized light treatment and the accompanying host immune response.In this stu...Photodynamic therapy(PDT)has garnered significant attention as a promising approach to cancer therapy,harnessing the combined benefits of localized light treatment and the accompanying host immune response.In this study,we engineered an immuno-enhanced PDT nanoplatform,denoted as HM@p-MOF(hybrid membrane@porphyrin-metal organic framework).The core porphyrin-MOF was cloaked with a hybrid membrane derived from B16F10 cancer cells and NK cells,resulting in enhanced stability.In both in vitro and in vivo experiments,our finding demonstrated that the hybrid membrane conferred dual targeting capabilities to the nanoplatform,leveraging the unique properties of the B16F10 membrane and NK membrane to augment immunogenic cell death(ICD)induced by photodynamic effects.Additionally,in conjunction with the immunomodulatory functions of the NK cell membrane,we observed an expansion of in situ immune infiltration leading to a systemic immune response.The HM@p-MOF nanoplatform exhibited the capacity to not only inhibit the growth of mouse melanoma but also suppress metastasis.This innovative HM@p-MOF nanoplatform present a viable strategy to enhance phototherapeutic efficacy for both localized and metastatic tumors.It provides a direction for the fabrication of biomimetic nanomedicines possessing immuno-modulatory function.展开更多
Combining phototherapy and chemotherapy has been considered a promising modality for cancer therapy due to their synergistic effect.Herein,we developed three D-π-A-structured boron dipyrromethenes(BODIPYs)(named as B...Combining phototherapy and chemotherapy has been considered a promising modality for cancer therapy due to their synergistic effect.Herein,we developed three D-π-A-structured boron dipyrromethenes(BODIPYs)(named as B-B,B-C,and B-C-Pt).Due to their enlargedπ-conjugated structure and high intramolecular charge transfer effect,the synthesized BODIPYs had photothermal conversion capability,and their absorption and fluorescence spectra were red-shifted.The cisplatin-appended BODIPY(B-C-Pt)exhibited good singlet oxygen(^1O_(2))generation ability and near infrared(NIR)absorption and fluorescence(λ_(Abs)=748 nm,λ_(Em)=947 nm).After being encapsulated by distearoyl phosphoethanolamine polyethyleneglycol 2000(DSPE-PEG-2000),which could inhibit the H-aggregation of B-C-Pt,the absorption and fluorescence of the obtained B-C-Pt nanoparticles(NPs)were red-shifted to 762 and 985 nm,respectively.The^1O_(2)quantum yield and photothermal conversion efficiency of the B-C-Pt NPs were 4.0%and 40.6%,respectively.Moreover,B-C-Pt NPs had chemotherapeutic efficacy due to the presence of cisplatin.In vitro and in vivo studies further demonstrated that B-C-Pt NPs had synergistic therapeutic efficacy.Together,B-C-Pt NPs could be employed in NIRⅡfluorescent and photoacoustic imaging-guided synergistic phototherapy and chemotherapy for cancer treatment.展开更多
Chemodynamic therapy(CDT)combined with dual phototherapy(photothermal therapy(PTT)and photodynamic therapy(PDT))is an efficient way to synergistically improve anti-tumor efficacy.However,the combination of multiple mo...Chemodynamic therapy(CDT)combined with dual phototherapy(photothermal therapy(PTT)and photodynamic therapy(PDT))is an efficient way to synergistically improve anti-tumor efficacy.However,the combination of multiple modes often makes the composition of the system more complex,which is not conducive to clinical application.In this study,a dual phototherapy ligand carboxyl-modified Aza-BODIPY(BOD-COOH)and metal active center Cu^(2+)were used to construct multiple-modes metalphotosensitizer nanoparticles(BOD-Cu NPs)via one-step coordination self-assembly for combination therapy of CDT/PDT/PTT.In order to improve delivery efficiency,the targeted hydrophilic molecule pyridinemodified glucose derivative(G-Py)was synthesized and coated onto the BOD-Cu NPs to form a glycosylated nano metal-photosensitizer BOD-Cu@G by electrostatic interaction.The Cu^(2+)in BOD-Cu@G could not only be used as a coordination node for metal-driven self-assembly but also consume intracellular glutathione(GSH),and then catalyze Fenton-like reaction to generate hydroxyl radical(·OH)for CDT.In vitro and in vivo studies revealed that BOD-Cu@G could achieve excellent anti-tumor efficiency by CDTenhanced dual phototherapy.展开更多
Polyoxometalates(POMs)are molecular metal-oxide clusters with precise chemical composition and architecture.Besides their bioactivities,electron-rich POMs have shown potential for enhancing synergistic therapy,such as...Polyoxometalates(POMs)are molecular metal-oxide clusters with precise chemical composition and architecture.Besides their bioactivities,electron-rich POMs have shown potential for enhancing synergistic therapy,such as photothermal therapy(PTT),photodynamic therapy(PDT),and chemo-dynamic therapy(CDT),through near-infrared region(NIR)absorption and redox reactions.展开更多
The integration of robust photon-absorption capacity,high reactive oxygen speciesyields and photothermal conversion efficiency(PCE)into a single phototheranosticnano-agents is ideal but rarely reported.This study empl...The integration of robust photon-absorption capacity,high reactive oxygen speciesyields and photothermal conversion efficiency(PCE)into a single phototheranosticnano-agents is ideal but rarely reported.This study employed a dual-acceptorengineering strategy utilizing isoindigo and selenium-substituted[1,2,5]thiadiazolo[3,4-c]pyridine to augment the molar extinction coefficient and spin-orbitcoupling effect,respectively,resulting in a substantial enhancement of photonabsorptionability and non-radiative decay energy-release process of donoracceptortype phototherapy molecules.As the optimal phototherapy agent,IID-PSe exhibited a high molar extinction coefficient two times that of photosensitizer,excellent 1O2 yield(15%)and PCE(34%),exhibiting great potential forphototherapy.After encapsulating with DSPE-PEG2000,IID-PSe NPs showedexcellent anti-tumor phototherapy ability both in vitro and in vivo.This workprovides an effective idea for designing high-performance photosensitive dyeswith high efficiency phototherapy output.展开更多
The tumor microenvironment(TME)promotes pro-tumor and anti-infammatory metabolismsand suppresses the host immune system.It prevents immune cells from fighting against cancereffectively,resulting in limited efficacy of...The tumor microenvironment(TME)promotes pro-tumor and anti-infammatory metabolismsand suppresses the host immune system.It prevents immune cells from fighting against cancereffectively,resulting in limited efficacy of many current cancer treatment modalities.Differenttherapies aim to overcome the immunosuppressive TME by combining various approaches tosynergize their effects for enhanced antitumor activity and augmented stimulation of the im-mune system.Immunotherapy has become a major therapeutic strategy because it unleashes thepower of the immune system by activating,enhancing,and directing immune responses to pre-vent,control,and eliminate cancer.Phototherapy uses light iradiation to induce tumor celldeath through photothermal,photochemical,and photo-immunological interactions.Photo-therapy induces tumor immunogenic cell death,which is a precursor and enhancer for anti-tumorimmunity.However,phototherapy alone has limited effects on long-term and systemic anti-tumor immune responses.Phototherapy can be combined with immunotherapy to improve thetumoricidal effect by killing target tumor cells,enhancing immune cell infiltration in tumors,andrewiring pathways in the TME from antinfammatory to pro-inflammatory.Phototherapy-enhanced immunotherapy triggers effective cooperation bet ween innate and adaptive immunities,specifically targeting the tumor cells,whether they are localized or distant.Herein,the successes and limitations of phototherapy combined with other cancer treatmentmodalities will be discussed.Specifically,we will review the symergistic effects of phototherapy combined with different cancer therapies on tumor elimination and remodeling of the immuno-suppressive TME.Overll,phototherapy,in combination with other therapeutic modalities,canestablish anti-tumor pro-infammatory phenotypes in activated tumor-infiltrating T cells andB cells and activate systemic anti-tumor immune responses.展开更多
Early surgical resection and chemotherapy of bone cancer are commonly used in the treatment of bone tumor,but it is still highly challenging to prevent recurrence and fill the bone defect caused by the resection site....Early surgical resection and chemotherapy of bone cancer are commonly used in the treatment of bone tumor,but it is still highly challenging to prevent recurrence and fill the bone defect caused by the resection site.In this work,we report a rational integration of photonic-responsive two-dimensional(2D)ultrathin niobium carbide(Nb2C)MXene nanosheets(NSs)into the 3D-printed bone-mimetic scaffolds(NBGS)for osteosarcoma treatment.The integrated 2D Nb2C-MXene NSs feature specific photonic response in the second near-infrared(NIR-II)biowindow with high tissue-penetrating depth,making it highly efficient in killing bone cancer cells.Importantly,Nb-based species released by the biodegradation of Nb2C MXene can obviously promote the neogenesis and migration of blood vessels in the defect site,which can transport more oxygen,vitamins and energy around the bone defect for the reparative process,and gather more immune cells around the defect site to accelerate the degradation of NBGS.The degradation of NBGS provides sufficient space for the bone remodeling.Besides,calcium and phosphate released during the degradation of the scaffold can promote the mineralization of new bone tissue.The intrinsic multifunctionality of killing bone tumor cell and promoting angiogenesis and bone regeneration makes the engineered Nb2C MXeneintegrated composite scaffolds a distinctive implanting biomaterial on the efficient treatment of bone tumor.展开更多
The enzyme-mediated elevation of reactive oxygen species(ROS)at the tumor sites has become an emerging strategy for regulating intracellular redox status for anticancer treatment.Herein,we proposed a camouflaged bioni...The enzyme-mediated elevation of reactive oxygen species(ROS)at the tumor sites has become an emerging strategy for regulating intracellular redox status for anticancer treatment.Herein,we proposed a camouflaged bionic cascaded-enzyme nanoreactor based on Ti_(3)C_(2)nanosheets for combined tumor enzyme dynamic therapy(EDT),phototherapy and deoxygenation-activated chemotherapy.Briefly,glucose oxidase(GOX)and chloroperoxidase(CPO)were chemically conjugated onto Ti_(3)C_(2)nanosheets,where the deoxygenation-activated drug tirapazamine(TPZ)was also loaded,and the Ti_(3)C_(2)-GOX-CPO/TPZ(TGCT)was embedded into nanosized cancer cell-derived membrane vesicles with high-expressed CD47(m_eTGCT).Due to biomimetic membrane camouflage and CD47 overexpression,m_eTGCT exhibited superior immune escape and homologous targeting capacities,which could effectively enhance the tumor preferential targeting and internalization.Once internalized into tumor cells,the cascade reaction of GOX and CPO could generate HClO for efficient EDT.Simultaneously,additional laser irradiation could accelerate the enzymic-catalytic reaction rate and increase the generation of singlet oxygen(~1O_(2)).Furthermore,local hypoxia environment with the oxygen depletion by EDT would activate deoxygenation-sensitive prodrug for additional chemotherapy.Consequently,m_eTGCT exhibits amplified synergistic therapeutic effects of tumor phototherapy,EDT and chemotherapy for efficient tumor inhibition.This intelligent cascaded-enzyme nanoreactor provides a promising approach to achieve concurrent and significant antitumor therapy.展开更多
Growing evidence suggests that the presence of cancer stem cells(CSCs)is a major challenge in current tumor treatments,especially the transition from non-CSCs to differentiation of CSCs for evading conventional therap...Growing evidence suggests that the presence of cancer stem cells(CSCs)is a major challenge in current tumor treatments,especially the transition from non-CSCs to differentiation of CSCs for evading conventional therapies and driving metastasis.Here we propose a therapeutic strategy of synergistic differentiation therapy and phototherapy to induce differentiation of CSCs into mature tumor cells by differentiation inducers and synergistic elimination of them and normal cancer cells through phototherapy.In this work,we synthesized a biomimetic nanoplatform loaded with IR-780 and all-trans retinoic acid(ATRA)via biomineralization.This method can integrate aluminum ions into small-sized protein carriers to form nanoclusters,which undergo responsive degradation under acidic conditions and facilitate deep tumor penetration.With the help of CSC differentiation induced by ATRA,IR-780 inhibited the self-renewal of CSCs and cancer progression by generating hyperthermia and reactive oxygen species in a synergistic manner.Furthermore,ATRA can boost immunogenic cell death induced by phototherapy,thereby strongly causing a systemic anti-tumor immune response and efficiently eliminating CSCs and tumor cells.Taken together,this dual strategy represents a new paradigm of targeted eradication of CSCs and tumors by inducing CSC differentiation,improving photothermal therapy/photodynamic therapy and enhancing antitumor immunity.展开更多
Background: At-home phototherapy devices for the treatment of acne have emerged as an appealing treatment option and as an effective adjunct treatment to existing modalities. The principal goal of the study was to det...Background: At-home phototherapy devices for the treatment of acne have emerged as an appealing treatment option and as an effective adjunct treatment to existing modalities. The principal goal of the study was to determine the changes in the number of inflammatory lesions in acne patients. Methods: Patients received instruction for daily at-home use of Silk’n Blue device for 12 weeks. Follow-up visits were conducted at 1 and 3 months to collect data. Results: Fifteen subjects with mild to severe cases experienced improvement over the course of the trial. The decrease in mean inflammatory acne counts (from 41.26 to 24.46) and mean percent reduction (41.8%) were statistically significant (p < 0.001). Some participants experienced percent reductions as great as 67%. No adverse events were recorded. Conclusions: The Silk’n Blue device is a safe and effective modality for at-home treatment of mild, moderate, and severe inflammatory acne vulgaris with proper use.展开更多
Corneal neovascularization(CNV) can induce severe visual impairment and even blindness.Current treatments have limited efficacy and some undesirable side effects.Phototherapy(FT) is a modern medical technique in CNV t...Corneal neovascularization(CNV) can induce severe visual impairment and even blindness.Current treatments have limited efficacy and some undesirable side effects.Phototherapy(FT) is a modern medical technique in CNV treatment and worth further improvement.Nanotechnology has various advantages and nanoparticle-mediated drug delive ry also contributes to CNV elimination.Phototherapy combined with functional nanoparticles featuring photoacoustic imaging contrast properties can accomplish accurate and safe inhibition of CNV,thus is promising in clinical application.展开更多
BACKGROUND The management of severe extravasation injuries is still controversial.Extravasation injuries can be treated in many ways.AIM To present a series of patients with severe extravasation injuries due to infusi...BACKGROUND The management of severe extravasation injuries is still controversial.Extravasation injuries can be treated in many ways.AIM To present a series of patients with severe extravasation injuries due to infusion who were managed with ethacridine lactate dressing combined with localized closure and phototherapy.METHODS In this study,we evaluated the data of eight patients,including six from the Department of Burn,one(with colorectal carcinoma)from the Veteran Cadre Department,and one(with leukemia)from the Hematology Department.Of these,three patients were male and five were female.Age of the patients ranged from 10 mo to 72 years,including two children(10 and 19 mo of age).In this study,the infusion was stopped immediately when the extravasation was identified.The extravasation event was managed routinely using a blocking solution.A ring-shaped localized closure was performed using the blocking agents.Moreover,ethacridine lactate dressing and phototherapy were applied for 3-5 d.RESULTS In this study,the drugs contained in the infusates were iodixanol,norepinephrine,alprostadil,amino acids,fat emulsion,cefoselis,cefoxitin,and potassium chloride+concentrated sodium chloride.All of the patients achieved complete healing after treatment and no obvious adverse reactions were observed.CONCLUSION The treatment of severe extravasation injuries using a combination of localized closure,ethacridine lactate dressing,and phototherapy resulted in satisfactory outcomes in patients.展开更多
In this paper,we studied portable blue and red light-emitting-diode(LED)light sources in phototherapy for mild to moderate acne vulgaris to evaluate the efficacy and tolerance of patients.Patients,randomly divided int...In this paper,we studied portable blue and red light-emitting-diode(LED)light sources in phototherapy for mild to moderate acne vulgaris to evaluate the efficacy and tolerance of patients.Patients,randomly divided into blue and red groups,received either blue or red LED phototherapy twice a week for four weeks.After complete treatment,the number of lesions reduced by 71.4% in the blue group,in contrast to 19.5% in the red group.No obvious side effects were observed during and one month after the treatment,except for some mild dryness mentioned by several patients.展开更多
Phototherapy lamps are the most effective and safest in the treatment of several medical treatments such as bilirubin. They transfer radiant energy expressed by irradiance unit in W/m2 into the skin specifically to ac...Phototherapy lamps are the most effective and safest in the treatment of several medical treatments such as bilirubin. They transfer radiant energy expressed by irradiance unit in W/m2 into the skin specifically to achieve a therapeutic reduction in the bilirubin concentration in the blood. National Institute for Standards （NIS） in Egypt builds up a radiometric method to en- sure the competence of phototherapy sources （luminaire） to the standard international and national requirements. Hence, NIS provides traceability to customer through unbroken chain of phototherapy radiometer calibrated as irradiance response in W/m2. Uncertainty model including all parameters accompanied with the measurements is studied.展开更多
An evaluation method for the practical photo-protection of eye-patch protectors (EPs) used in phototherapy for neonatal jaundice is developed.The protecting condition of neonatal eyes using EPs under typical photother...An evaluation method for the practical photo-protection of eye-patch protectors (EPs) used in phototherapy for neonatal jaundice is developed.The protecting condition of neonatal eyes using EPs under typical phototherapy light is simulated.The simulation system consists of a neonatal head manikin,an International Light ILT 900 wideband rapid portable spectroradiometer,a standard Air Shields Isolette incubator,and a Medela fluorescent phototherapy unit.The results show that the practical protection of neonatal eyes,provided by EPs which are affected by the material characteristic,shape design,and wearing condition,is comprehensively illustrated using the indices of illuminance (Ev in lux unit) and the spectral irradiance (E in μW/(cm2·nm) unit).展开更多
Objective To investigate the effects of phototherapy combined with albumin administration on hyperbilirubinemia in newborns. Methods Totally 64 newborns with neonatal hyperbilirubinemia were randomly divided into the ...Objective To investigate the effects of phototherapy combined with albumin administration on hyperbilirubinemia in newborns. Methods Totally 64 newborns with neonatal hyperbilirubinemia were randomly divided into the control group and the study group,with 32 cases in each group. The newborns in control group received phototherapy and routine care,while those in study group were administrated with intravenous injection of albumin in addition to phototherapy and routine care. The concentration of total serum bilirubin,newborns' body weight and amount of milk intake were measured before and after phototherapy. Results There was a significant difference in the milk intake between two groups after phototherapy( P < 0. 05). There was no significant difference in the body weight or total serum bilirubin level of newborns between two groups after phototherapy( P > 0. 05). Conclusion Combined application of phothotherapy and albumin administration on neonatal hyperbilirubinemia could promote the newborns' appetite and improve the recovery of the neonates,but the effect on reducing blood bilirubin level and weight gain needs further study.展开更多
基金supported by the National Natural Science Foundation of China(Nos.22078201,U1908202)Liaoning&Shenyang Key Laboratory of Functional Dye and Pigment(Nos.2021JH13/10200018,21-104-0-23,LJKZ0453)China Medical University’s High-Quality Development Science and Technology Funding Program(Nos.2022JH2/20200063,2023JH2/20200162).
文摘Therapy-induced modulation of the tumor microenvironment(TME)to overcome the immunosuppressive TME is considered to be a chance for cancer treatment.Herein,we prepared near-infrared absorbing aza-BODIPY PhEt-azaBDP with 1-phenylethyl group at 1,7-sites,a type I photodynamic-photothermal therapy(PDT-PTT)agent.Self-assembly PhEt-azaBDP nanoparticles(NPs)can provide combined phototherapeutic effects under light irradiation and simultaneously induce inflammatory TME,by monitoring tumorassociated macrophages(TAMs)repolarization.Utilizing cluster of differentiation 86(CD86)and CD163 as the M1-type marker and M2-type marker respectively,PhEt-azaBDP NPs resulted in the increasement of the expression of CD86 and the decreasement of the expression of CD163 in TAMs under near-infrared(NIR)light irradiation,promoting TAMs to switch from M2-phenotype to M1-phenotype.Inflammatory cytokines,interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α),could be the key cytokine involved in the phototherapy-induced TME reprogramming.PhEt-azaBDP NPs could be a potential theranostic scaffold for the simultaneous induction and detection of TME reprogramming triggered by phototherapy.
文摘Rational design of multifunctional nanoplatforms capable of combining therapeutic effects with real-time monitoring of drug distribution and tumor status is emerging as a promising approach in cancer nanomedicine.Here,we introduce pyropheophorbide a-bisaminoquinoline conjugate lipid nanoparticles(PPBC LNPs)as a bimodal system for image-guided phototherapy in bladder cancer treatment.PPBC LNPs not only demonstrate both powerful photodynamic and photothermal effects upon light activation,but also exhibit potent autophagy blockage,effectively inducing bladder cancer cell death.Furthermore,PPBC LNPs possess remarkable photoacoustic(PA)and fluorescence(FL)imaging capabilities,enabling imaging with high-resolution,deep tissue penetration and high sensitivity for tracking drug biodistribution and phototherapy efficacy.Specifically,PA imaging confirms the efficient accumulation of PPBC LNPs within tumor and predicts therapeutic outcomes of photodynamic therapy,while FL imaging confirms their prolonged retention at the tumor site for up to 6 days.PPBC LNPs significantly suppress bladder tumor growth,with several tumors completely ablated following just two doses of the nanoparticles and laser treatment.Additionally,PPBC LNPs were formulated with lipid-based excipients and assembled using microfluidic technology to enhance biocompatibility,stability,and scalability,showing potential for clinical translation.This versatile nanoparticle represents a promising candidate for further development in bladder cancer therapy.
基金Supported by the Tianjin Municipal Education Commission Scientic Research Project No.2023YXZD10。
文摘The hippocampus is the brain structure that is responsible for the formation of learning memories.Sleep disorders leading to cognitive impairment are strongly associated with the hippocampus.Phototherapy offers a new physical therapy for the treatment of sleep disorders,with the advantages of being noninvasive and having few side effects.However,the mechanism by which phototherapy improves cognitive impairment caused by sleep disorders remains unclear.In this study,we used phototherapy combined with optogenetic technology to investigate the effect of noninvasive phototherapy on cognitive functions in sleep-deprived mice.Our results suggest that phototherapy might improve cognitive functions in sleep-deprived mice by modulating the hippocampus.Our study expands the research progress on noninvasive phototherapy for the treatment of sleep disorders.
基金supported by the National Key Research and Development Program of China(No.2022YFA1207600)the National Natural Science Foundation of China(Nos.62375289,62175262)+2 种基金the Science and Technology Innovation Program of Hunan Province(No.2022RC1201)the Scientific Research Fund of Hunan Provincial Education Department(No.22B0081)Postdoctoral Funding Project of Jiangsu Province(No.2019Z156)。
文摘Designing and synthesizing nanomedicines with multi-modal tumor therapeutic capabilities is the key to cancer treatment.Herein,we prepared MICG nanoparticles(NPs)by assembling glucose oxidase(GOx)and indocyanine green(ICG)with manganese carbonate(MnCO_(3))NPs for starvation therapy cascaded chemodynamic therapy,enhanced phototherapy and immune activation.In MICG NPs,the GOx consumes intratumoral glucose resulting in starvation therapy,and simultaneously produces H_(2)O_(2)and decreases p H in tumor.The intensified acidic tumor environment promotes the decomposition of MnCO_(3)NPs to release Mn^(2+).The Mn^(2+)further catalyzes H_(2)O_(2)to generate hydroxyl radical for chemodynamic therapy.While ICG can generate singlet oxygen(^(1)O_(2))and heat to kill cancer cells through phototherapy mechanism.The hydroxyl radical and ^(1)O_(2) will further accelerate the oxidative stress,intensify immunogenic cell death,induce dendritic cell maturation,and thus activate systemic immunity.This work provides a new therapeutic platform for combining therapy of tumor.
基金supported by the National Natural Science Foundation of China(Nos.22175107,22305140,51603184,82270531)Zhejiang Provincial Natural Science Foundation of China(Nos.LY21E030002,LY22H020003)+1 种基金Scientific Research Foundation of Zhejiang University City College(No.J-202104)Hangzhou Science and Technology Bureau of China(No.202203B27)。
文摘Photodynamic therapy(PDT)has garnered significant attention as a promising approach to cancer therapy,harnessing the combined benefits of localized light treatment and the accompanying host immune response.In this study,we engineered an immuno-enhanced PDT nanoplatform,denoted as HM@p-MOF(hybrid membrane@porphyrin-metal organic framework).The core porphyrin-MOF was cloaked with a hybrid membrane derived from B16F10 cancer cells and NK cells,resulting in enhanced stability.In both in vitro and in vivo experiments,our finding demonstrated that the hybrid membrane conferred dual targeting capabilities to the nanoplatform,leveraging the unique properties of the B16F10 membrane and NK membrane to augment immunogenic cell death(ICD)induced by photodynamic effects.Additionally,in conjunction with the immunomodulatory functions of the NK cell membrane,we observed an expansion of in situ immune infiltration leading to a systemic immune response.The HM@p-MOF nanoplatform exhibited the capacity to not only inhibit the growth of mouse melanoma but also suppress metastasis.This innovative HM@p-MOF nanoplatform present a viable strategy to enhance phototherapeutic efficacy for both localized and metastatic tumors.It provides a direction for the fabrication of biomimetic nanomedicines possessing immuno-modulatory function.
基金supported by National Natural Science Foundation of China(No.62175262)the Science and Technology Innovation Program of Hunan Province(No.2022RC1201)the Fundamental Research Funds for the Central Universities of Central South University(No.2019zzts849)。
文摘Combining phototherapy and chemotherapy has been considered a promising modality for cancer therapy due to their synergistic effect.Herein,we developed three D-π-A-structured boron dipyrromethenes(BODIPYs)(named as B-B,B-C,and B-C-Pt).Due to their enlargedπ-conjugated structure and high intramolecular charge transfer effect,the synthesized BODIPYs had photothermal conversion capability,and their absorption and fluorescence spectra were red-shifted.The cisplatin-appended BODIPY(B-C-Pt)exhibited good singlet oxygen(^1O_(2))generation ability and near infrared(NIR)absorption and fluorescence(λ_(Abs)=748 nm,λ_(Em)=947 nm).After being encapsulated by distearoyl phosphoethanolamine polyethyleneglycol 2000(DSPE-PEG-2000),which could inhibit the H-aggregation of B-C-Pt,the absorption and fluorescence of the obtained B-C-Pt nanoparticles(NPs)were red-shifted to 762 and 985 nm,respectively.The^1O_(2)quantum yield and photothermal conversion efficiency of the B-C-Pt NPs were 4.0%and 40.6%,respectively.Moreover,B-C-Pt NPs had chemotherapeutic efficacy due to the presence of cisplatin.In vitro and in vivo studies further demonstrated that B-C-Pt NPs had synergistic therapeutic efficacy.Together,B-C-Pt NPs could be employed in NIRⅡfluorescent and photoacoustic imaging-guided synergistic phototherapy and chemotherapy for cancer treatment.
基金supported by the National Natural Science Foundation of China(No.22171230)the Project of Science and Technology of Social Development in Shaanxi Province(No.2023YBSF-151)。
文摘Chemodynamic therapy(CDT)combined with dual phototherapy(photothermal therapy(PTT)and photodynamic therapy(PDT))is an efficient way to synergistically improve anti-tumor efficacy.However,the combination of multiple modes often makes the composition of the system more complex,which is not conducive to clinical application.In this study,a dual phototherapy ligand carboxyl-modified Aza-BODIPY(BOD-COOH)and metal active center Cu^(2+)were used to construct multiple-modes metalphotosensitizer nanoparticles(BOD-Cu NPs)via one-step coordination self-assembly for combination therapy of CDT/PDT/PTT.In order to improve delivery efficiency,the targeted hydrophilic molecule pyridinemodified glucose derivative(G-Py)was synthesized and coated onto the BOD-Cu NPs to form a glycosylated nano metal-photosensitizer BOD-Cu@G by electrostatic interaction.The Cu^(2+)in BOD-Cu@G could not only be used as a coordination node for metal-driven self-assembly but also consume intracellular glutathione(GSH),and then catalyze Fenton-like reaction to generate hydroxyl radical(·OH)for CDT.In vitro and in vivo studies revealed that BOD-Cu@G could achieve excellent anti-tumor efficiency by CDTenhanced dual phototherapy.
基金supported by the National Natural Science Foundation of China(Grant Nos.:92261203,21971106,22171073,22101118,and 22201123)the Central Guided Science and Technology Development Foundation of Liaoning Province,China(Grant No.:2022 JH6/100100036)+1 种基金the Start-up Fund from Southern University of Science and Technology(SUSTech),China,the Stable Support Plan Program of Shenzhen Natural Science Fund,China(Program Contract No.:20200925161141006)the Shenzhen Nobel Prize Scientists Laboratory Project(Shenzhen Grubbs Institute),China(Project No.:C17783101).
文摘Polyoxometalates(POMs)are molecular metal-oxide clusters with precise chemical composition and architecture.Besides their bioactivities,electron-rich POMs have shown potential for enhancing synergistic therapy,such as photothermal therapy(PTT),photodynamic therapy(PDT),and chemo-dynamic therapy(CDT),through near-infrared region(NIR)absorption and redox reactions.
基金supported by the National Natural Science Foundation of China(21925802,22338005,22308049)Liaoning Binhai Laboratory(LBLB-2023-03)+1 种基金the Fundamental Research Funds for the Central Universities(DUT22LAB601)the Postdoctoral ResearchFoundation of Ningbo Institute of Dalian University ofTechnology(03020002).
文摘The integration of robust photon-absorption capacity,high reactive oxygen speciesyields and photothermal conversion efficiency(PCE)into a single phototheranosticnano-agents is ideal but rarely reported.This study employed a dual-acceptorengineering strategy utilizing isoindigo and selenium-substituted[1,2,5]thiadiazolo[3,4-c]pyridine to augment the molar extinction coefficient and spin-orbitcoupling effect,respectively,resulting in a substantial enhancement of photonabsorptionability and non-radiative decay energy-release process of donoracceptortype phototherapy molecules.As the optimal phototherapy agent,IID-PSe exhibited a high molar extinction coefficient two times that of photosensitizer,excellent 1O2 yield(15%)and PCE(34%),exhibiting great potential forphototherapy.After encapsulating with DSPE-PEG2000,IID-PSe NPs showedexcellent anti-tumor phototherapy ability both in vitro and in vivo.This workprovides an effective idea for designing high-performance photosensitive dyeswith high efficiency phototherapy output.
基金supported in part by the National Cancer Institute(R01CA205348 and R01CA269897)the Oklahoma Center for the Advancement of Science and Technology(HR16-085 and HF20-019)+1 种基金supported in part by the Institutional Development Award(IDeA)from the National Institute of General Medical Sciences,National Institutes of Health,under Grant Number P20GM135009Trisha I.Valerio and Coline L.Furrer contributed equally to this work.
文摘The tumor microenvironment(TME)promotes pro-tumor and anti-infammatory metabolismsand suppresses the host immune system.It prevents immune cells from fighting against cancereffectively,resulting in limited efficacy of many current cancer treatment modalities.Differenttherapies aim to overcome the immunosuppressive TME by combining various approaches tosynergize their effects for enhanced antitumor activity and augmented stimulation of the im-mune system.Immunotherapy has become a major therapeutic strategy because it unleashes thepower of the immune system by activating,enhancing,and directing immune responses to pre-vent,control,and eliminate cancer.Phototherapy uses light iradiation to induce tumor celldeath through photothermal,photochemical,and photo-immunological interactions.Photo-therapy induces tumor immunogenic cell death,which is a precursor and enhancer for anti-tumorimmunity.However,phototherapy alone has limited effects on long-term and systemic anti-tumor immune responses.Phototherapy can be combined with immunotherapy to improve thetumoricidal effect by killing target tumor cells,enhancing immune cell infiltration in tumors,andrewiring pathways in the TME from antinfammatory to pro-inflammatory.Phototherapy-enhanced immunotherapy triggers effective cooperation bet ween innate and adaptive immunities,specifically targeting the tumor cells,whether they are localized or distant.Herein,the successes and limitations of phototherapy combined with other cancer treatmentmodalities will be discussed.Specifically,we will review the symergistic effects of phototherapy combined with different cancer therapies on tumor elimination and remodeling of the immuno-suppressive TME.Overll,phototherapy,in combination with other therapeutic modalities,canestablish anti-tumor pro-infammatory phenotypes in activated tumor-infiltrating T cells andB cells and activate systemic anti-tumor immune responses.
基金the financial support from the National Key R&D Program of China(Grant No.2016YFA0203700)the National Natural Science Foundation of China(Grant Nos.51872185,51722211,51672303,81672131,81672143,82072417 and 81802247)+2 种基金the Program of Shanghai Academic Research Leader(Grant No.18XD1404300)the National Key Research and Development Project of China(Grant No.2018YFC1106303)the Science and Technology Commission of Shanghai Municipality(Grant No.17060502400).
文摘Early surgical resection and chemotherapy of bone cancer are commonly used in the treatment of bone tumor,but it is still highly challenging to prevent recurrence and fill the bone defect caused by the resection site.In this work,we report a rational integration of photonic-responsive two-dimensional(2D)ultrathin niobium carbide(Nb2C)MXene nanosheets(NSs)into the 3D-printed bone-mimetic scaffolds(NBGS)for osteosarcoma treatment.The integrated 2D Nb2C-MXene NSs feature specific photonic response in the second near-infrared(NIR-II)biowindow with high tissue-penetrating depth,making it highly efficient in killing bone cancer cells.Importantly,Nb-based species released by the biodegradation of Nb2C MXene can obviously promote the neogenesis and migration of blood vessels in the defect site,which can transport more oxygen,vitamins and energy around the bone defect for the reparative process,and gather more immune cells around the defect site to accelerate the degradation of NBGS.The degradation of NBGS provides sufficient space for the bone remodeling.Besides,calcium and phosphate released during the degradation of the scaffold can promote the mineralization of new bone tissue.The intrinsic multifunctionality of killing bone tumor cell and promoting angiogenesis and bone regeneration makes the engineered Nb2C MXeneintegrated composite scaffolds a distinctive implanting biomaterial on the efficient treatment of bone tumor.
基金This work was supported by the National Natural Science Foundation of China(51773231)Shenzhen Science and Technology Project(JCYJ20190807160801664)the Project of Key Laboratory of Sensing Technology and Biomedical Instruments of Guangdong Province(2011A060901013).
文摘The enzyme-mediated elevation of reactive oxygen species(ROS)at the tumor sites has become an emerging strategy for regulating intracellular redox status for anticancer treatment.Herein,we proposed a camouflaged bionic cascaded-enzyme nanoreactor based on Ti_(3)C_(2)nanosheets for combined tumor enzyme dynamic therapy(EDT),phototherapy and deoxygenation-activated chemotherapy.Briefly,glucose oxidase(GOX)and chloroperoxidase(CPO)were chemically conjugated onto Ti_(3)C_(2)nanosheets,where the deoxygenation-activated drug tirapazamine(TPZ)was also loaded,and the Ti_(3)C_(2)-GOX-CPO/TPZ(TGCT)was embedded into nanosized cancer cell-derived membrane vesicles with high-expressed CD47(m_eTGCT).Due to biomimetic membrane camouflage and CD47 overexpression,m_eTGCT exhibited superior immune escape and homologous targeting capacities,which could effectively enhance the tumor preferential targeting and internalization.Once internalized into tumor cells,the cascade reaction of GOX and CPO could generate HClO for efficient EDT.Simultaneously,additional laser irradiation could accelerate the enzymic-catalytic reaction rate and increase the generation of singlet oxygen(~1O_(2)).Furthermore,local hypoxia environment with the oxygen depletion by EDT would activate deoxygenation-sensitive prodrug for additional chemotherapy.Consequently,m_eTGCT exhibits amplified synergistic therapeutic effects of tumor phototherapy,EDT and chemotherapy for efficient tumor inhibition.This intelligent cascaded-enzyme nanoreactor provides a promising approach to achieve concurrent and significant antitumor therapy.
基金supported by National Science and Technology Major Special Project-Major New Drug Creation(2019ZX09301-112)Shandong Natural Science Foundation(ZR2020QH351)+1 种基金Shandong Provincial Program of Taishan Industrial Experts(2019TSCYCX-31)the Fundamental Research Funds of Shandong University(2020GN091)
文摘Growing evidence suggests that the presence of cancer stem cells(CSCs)is a major challenge in current tumor treatments,especially the transition from non-CSCs to differentiation of CSCs for evading conventional therapies and driving metastasis.Here we propose a therapeutic strategy of synergistic differentiation therapy and phototherapy to induce differentiation of CSCs into mature tumor cells by differentiation inducers and synergistic elimination of them and normal cancer cells through phototherapy.In this work,we synthesized a biomimetic nanoplatform loaded with IR-780 and all-trans retinoic acid(ATRA)via biomineralization.This method can integrate aluminum ions into small-sized protein carriers to form nanoclusters,which undergo responsive degradation under acidic conditions and facilitate deep tumor penetration.With the help of CSC differentiation induced by ATRA,IR-780 inhibited the self-renewal of CSCs and cancer progression by generating hyperthermia and reactive oxygen species in a synergistic manner.Furthermore,ATRA can boost immunogenic cell death induced by phototherapy,thereby strongly causing a systemic anti-tumor immune response and efficiently eliminating CSCs and tumor cells.Taken together,this dual strategy represents a new paradigm of targeted eradication of CSCs and tumors by inducing CSC differentiation,improving photothermal therapy/photodynamic therapy and enhancing antitumor immunity.
文摘Background: At-home phototherapy devices for the treatment of acne have emerged as an appealing treatment option and as an effective adjunct treatment to existing modalities. The principal goal of the study was to determine the changes in the number of inflammatory lesions in acne patients. Methods: Patients received instruction for daily at-home use of Silk’n Blue device for 12 weeks. Follow-up visits were conducted at 1 and 3 months to collect data. Results: Fifteen subjects with mild to severe cases experienced improvement over the course of the trial. The decrease in mean inflammatory acne counts (from 41.26 to 24.46) and mean percent reduction (41.8%) were statistically significant (p < 0.001). Some participants experienced percent reductions as great as 67%. No adverse events were recorded. Conclusions: The Silk’n Blue device is a safe and effective modality for at-home treatment of mild, moderate, and severe inflammatory acne vulgaris with proper use.
基金This study was supported in part by the National Key R&D Program of China(Nos.2018YFA0107301 and 2018YFA0107304)the National Natural Science Foundation of China(NSFC,Nos.81770894,81901876,81470602 and 81330022)+2 种基金the Fundamental Research Funds for the Central Universities(Nos.20720190088 and 20720200019)the Medical and Health Key Project of Xiamen(No.3502Z20191106)the Program for New Century Excellent Talents in University,China(No.NCET-13-0502).
文摘Corneal neovascularization(CNV) can induce severe visual impairment and even blindness.Current treatments have limited efficacy and some undesirable side effects.Phototherapy(FT) is a modern medical technique in CNV treatment and worth further improvement.Nanotechnology has various advantages and nanoparticle-mediated drug delive ry also contributes to CNV elimination.Phototherapy combined with functional nanoparticles featuring photoacoustic imaging contrast properties can accomplish accurate and safe inhibition of CNV,thus is promising in clinical application.
基金Supported by Natural Science Foundation of Hunan Provincial Science and Technology Department,No.2019JJ40527.
文摘BACKGROUND The management of severe extravasation injuries is still controversial.Extravasation injuries can be treated in many ways.AIM To present a series of patients with severe extravasation injuries due to infusion who were managed with ethacridine lactate dressing combined with localized closure and phototherapy.METHODS In this study,we evaluated the data of eight patients,including six from the Department of Burn,one(with colorectal carcinoma)from the Veteran Cadre Department,and one(with leukemia)from the Hematology Department.Of these,three patients were male and five were female.Age of the patients ranged from 10 mo to 72 years,including two children(10 and 19 mo of age).In this study,the infusion was stopped immediately when the extravasation was identified.The extravasation event was managed routinely using a blocking solution.A ring-shaped localized closure was performed using the blocking agents.Moreover,ethacridine lactate dressing and phototherapy were applied for 3-5 d.RESULTS In this study,the drugs contained in the infusates were iodixanol,norepinephrine,alprostadil,amino acids,fat emulsion,cefoselis,cefoxitin,and potassium chloride+concentrated sodium chloride.All of the patients achieved complete healing after treatment and no obvious adverse reactions were observed.CONCLUSION The treatment of severe extravasation injuries using a combination of localized closure,ethacridine lactate dressing,and phototherapy resulted in satisfactory outcomes in patients.
文摘In this paper,we studied portable blue and red light-emitting-diode(LED)light sources in phototherapy for mild to moderate acne vulgaris to evaluate the efficacy and tolerance of patients.Patients,randomly divided into blue and red groups,received either blue or red LED phototherapy twice a week for four weeks.After complete treatment,the number of lesions reduced by 71.4% in the blue group,in contrast to 19.5% in the red group.No obvious side effects were observed during and one month after the treatment,except for some mild dryness mentioned by several patients.
文摘Phototherapy lamps are the most effective and safest in the treatment of several medical treatments such as bilirubin. They transfer radiant energy expressed by irradiance unit in W/m2 into the skin specifically to achieve a therapeutic reduction in the bilirubin concentration in the blood. National Institute for Standards （NIS） in Egypt builds up a radiometric method to en- sure the competence of phototherapy sources （luminaire） to the standard international and national requirements. Hence, NIS provides traceability to customer through unbroken chain of phototherapy radiometer calibrated as irradiance response in W/m2. Uncertainty model including all parameters accompanied with the measurements is studied.
基金Earmarked Research Grant(Hong Kong),China (No.POLYU5299/04E)
文摘An evaluation method for the practical photo-protection of eye-patch protectors (EPs) used in phototherapy for neonatal jaundice is developed.The protecting condition of neonatal eyes using EPs under typical phototherapy light is simulated.The simulation system consists of a neonatal head manikin,an International Light ILT 900 wideband rapid portable spectroradiometer,a standard Air Shields Isolette incubator,and a Medela fluorescent phototherapy unit.The results show that the practical protection of neonatal eyes,provided by EPs which are affected by the material characteristic,shape design,and wearing condition,is comprehensively illustrated using the indices of illuminance (Ev in lux unit) and the spectral irradiance (E in μW/(cm2·nm) unit).
基金Guangxi Medical and Health Plan Project(22015387)
文摘Objective To investigate the effects of phototherapy combined with albumin administration on hyperbilirubinemia in newborns. Methods Totally 64 newborns with neonatal hyperbilirubinemia were randomly divided into the control group and the study group,with 32 cases in each group. The newborns in control group received phototherapy and routine care,while those in study group were administrated with intravenous injection of albumin in addition to phototherapy and routine care. The concentration of total serum bilirubin,newborns' body weight and amount of milk intake were measured before and after phototherapy. Results There was a significant difference in the milk intake between two groups after phototherapy( P < 0. 05). There was no significant difference in the body weight or total serum bilirubin level of newborns between two groups after phototherapy( P > 0. 05). Conclusion Combined application of phothotherapy and albumin administration on neonatal hyperbilirubinemia could promote the newborns' appetite and improve the recovery of the neonates,but the effect on reducing blood bilirubin level and weight gain needs further study.
