Two new phloroglucinol glycosides, lysidiciside A (1) and lysidiciside B (2), were isolated from the roots of Lysidice rhodostega Hance. Their structures were determined as 1-[(3-methylbutyryl)phloroglucinol]-β-D- g...Two new phloroglucinol glycosides, lysidiciside A (1) and lysidiciside B (2), were isolated from the roots of Lysidice rhodostega Hance. Their structures were determined as 1-[(3-methylbutyryl)phloroglucinol]-β-D- glucopyranoside (1), 1- [(3-methylbutyryl)phloro- glucinol]-β-D-glucopyranosyl-5-O-β-D-glucopyranoside (2) on the basis of chemical and spectral analysis .展开更多
Objective: Diabetes is a common metabolic disease with several complications in its patients. Often, people living with diabetes develop erectile dysfunction (ED). The primary aim of this work was to investigate the e...Objective: Diabetes is a common metabolic disease with several complications in its patients. Often, people living with diabetes develop erectile dysfunction (ED). The primary aim of this work was to investigate the effect of phloroglucinol in diabetes-induced ED in rats. Methods: Male Wistar rats were given 52 mg/kg of streptozotocin, by intraperitoneal injection, to induce diabetes and ED. Subsequently, animals were grouped into three groups: group 1, diabetic control;group 2, low-dose phloroglucinol (150 mg/kg body weight);and group 3, high-dose phloroglucinol (250 mg/kg body weight). A group of six normal rats served as a normal control. The rats were treated with phloroglucinol for six weeks and then were assessed for treatment effects. Sexual behavior, glycosylated hemoglobin Ale (HbAlc) values, serum testosterone, serum nitric oxide (NO), blood pressure and sperm count were measured after the end of treatment. Results: After a 6-week treatment period, the high dose of phloroglucinol significantly decreased HbAlc values in diabetic rats. Rats treated with phloroglucinol had increased serum testosterone, NO and sperm count. Animals treated with 250 mg/kg phloroglucinol performed similar to normal rats in the sexual behavioral study, suggesting the reversal of complications of ED. Conversely, a decrease in the blood pressure in treated groups was observed. Conclusion: The results highlight the protective effect of phloroglucinol in diabetes-induced ED in rats warranting further studies.展开更多
A novel meroterpenoid,euphoractone(1),was isolated from the extracts of the roots of Euphorbia fischeriana Steud.Its structure was determined by spectroscopic methods and X-ray crystallography.1 possesses an unusual e...A novel meroterpenoid,euphoractone(1),was isolated from the extracts of the roots of Euphorbia fischeriana Steud.Its structure was determined by spectroscopic methods and X-ray crystallography.1 possesses an unusual ent-abietane-phloroglucinol skeleton.The plausible biosynthetic pathway for 1 was proposed.1 showed inhibitory activities against human lung cancer H23 and H460 cells with the IC_(50)values of 21.07±3.54 and 20.91±4.07 μmol/L.展开更多
To improve the purity of the total phloroglucinols from Dryopteris crassirhizoma extracts, the separation and purification conditions of the total phloroglucinols from Dryopteris crassirhizoma were studied with DM-130...To improve the purity of the total phloroglucinols from Dryopteris crassirhizoma extracts, the separation and purification conditions of the total phloroglucinols from Dryopteris crassirhizoma were studied with DM-130 macroporous adsorption resin in this study. Adsorption rate, elution rate and purity of the total phloroglucinols were used as indexes to investigate the adsorption and desorption capacity of the total phloroglucinols with DM-130 macroporous adsorption resin. Through the study, the optimum sample concentration of the total phloroglucinols and maximum sample volume were 1.5 mg·mL^-1 and 7 BV （210 mL）, respectively. The optimum desorption conditions were achieved by using 80% ethanol as desorption solvent at elution flow rate of 1.0 mL·min^-1. The result showed DM-130 macroporous adsorption resin performed effective adsorption and desorption. After purification, the purity of the total phloroglucinols increased by 11.5-fold.展开更多
Phytochemical investigation of the MeOH extract of twigs and leaves of Baeckea frutescens led to the isolation of seven new polymethylated phloroglucinol meroterpenoids(PPMs),named baeckfrutones M-S(1-7).Their structu...Phytochemical investigation of the MeOH extract of twigs and leaves of Baeckea frutescens led to the isolation of seven new polymethylated phloroglucinol meroterpenoids(PPMs),named baeckfrutones M-S(1-7).Their structures and absolute configurations were determined by spectroscopic analyses,chiral-phase HPLC analysis,and electronic circular dichroism(ECD)calculations.PPM 1 is a novel meroterpenoid possessing a 6/6/5/3 tetracyclic skeleton in PPMs,whereas 3 and 4 are the first hydroxytasmanone type phloroglucinol-monoterpene hybrids.(+)-2 and 7 displayed potent antiinflammatory activity with IC50 values of 20.86±0.60 and 36.21±1.18 lL,respectively.展开更多
Two new phloroglucinol glycosides,2,6-dihydroxy-3,5-dimethyl-1-isobutyrylbenzene-4-O-beta-D-glucoside and 2,6-dihydroxy-3,5-dimethyl-1-(2-methylbutyryl)benzene-4-O-beta-D-glucoside were isolated from Hypericum japonic...Two new phloroglucinol glycosides,2,6-dihydroxy-3,5-dimethyl-1-isobutyrylbenzene-4-O-beta-D-glucoside and 2,6-dihydroxy-3,5-dimethyl-1-(2-methylbutyryl)benzene-4-O-beta-D-glucoside were isolated from Hypericum japonicum.Their structures were determined by spectroscopic and chemical methods.展开更多
Background:Myocardial injury due to ischemia‐reperfusion(IR)is aggravated in diabetes which is associated with oxidative stress.Alleviating oxidative stress via use of antioxidants has been shown to be effective at m...Background:Myocardial injury due to ischemia‐reperfusion(IR)is aggravated in diabetes which is associated with oxidative stress.Alleviating oxidative stress via use of antioxidants has been shown to be effective at minimizing myocardial cell death and improving cardiac function.The aim of the present study was to evaluate the cardioprotective effect of phloroglucinol against myocardial reperfusion injury(MRI)in diabetic rats.Methods:Diabetes was induced in female rats with streptozotocin(50 mg/kg).The diabetic rats were orally treated with phloroglucinol(100 and 200 mg/kg daily for 28 days).After treatment the hearts were isolated and mounted on a Langendorff apparatus.The hearts were subjected to 15 minutes of IR to induce myocardial damage.Cardiac functions including heart rate(HR),resting and developed tension,and rate of change of contraction(+dP/dtmax)were recorded.Cardiac injury biomarkers lactate dehydrogenase(LDH)and creatine kinase(CK‐MB)were measured in the heart perfusate.Levels of the antioxidant enzymes reduced glutathione(GSH)and malondialdehyde(MDA)were measured.Hematoxylin and eosin(H&E)staining was also performed.Results:After IR injury,a decrease in HR and+dP/dtmax in hearts from diabetic rat was seen compared to healthy rat hearts,which was reversed by phloroglucinol treatment.Myocardial infarct size,measured by H&E staining,was increased in diabetic rats compared to healthy rats and an increase in the activity of LDH and CK‐MB in the heart perfusate in diabetic rats was decreased by phloroglucinol treatment.An increase in MDA levels and a decrease in levels of antioxidant enzymes were observed in diabetic rats,which was reversed with phloroglucinol treatment.Conclusion:Phloroglucinol treatment has potential therapeutic promise in the treatment of MRI in diabetes.展开更多
Background： Phloroglucinol plays an important role in oxidative stress and inflammatory responses. The effects of phloroglucinol have been proven in various disease models. The aim of the present study was to investi...Background： Phloroglucinol plays an important role in oxidative stress and inflammatory responses. The effects of phloroglucinol have been proven in various disease models. The aim of the present study was to investigate the efficacy and possible mechanisms of phloroglucinol in the treatment of interstitial cystitis （IC）. Methods： Thirty-two female Sprague-Dawley （SD） rats were used in this study. IC was induced by intraperitoneal injection of cyclophosphamide （CYP）. Rats were randomly allocated to one of four groups （n = 8 per group）： A control group, which was injected with saline （75 mg/kg; i.p.） instead of CYP on days 1, 4, and 7; a chronic IC group, which was injected with CYP （75 mg/kg; i.p.） on days l, 4, and 7; a high-dose （30 mg/kg） phloroglucinol-treated group; and a low-dose （15 mg/kg） phloroglucinol-treated group. On day 8, the rats in each group underwent cystometrography （CMG）, and the bladders were examined for evidence of oxidative stress and inflammation. Statistical analysis was performed by analysis of variance （ANOVA） followed by least square difference multiple comparison po.s＇t-hoc test. Results： Histological evaluation showed that bladder inflammation in CYP-treated rats was suppressed by phloroglucinol. CMG revealed that the CYP treatment induced overactive bladder in rats that was reversed by phloroglucinol. Up-regulated tumor necrosis factor-or and interleukin-6 expression in the CYP-treated rats were also suppressed in the phloroglucinol treated rats. CYP treatment significantly increased myeloperoxidase activity as well as the decreased activities ofcatalase of the bladder, which was reversed by treatment with phloroglucinol. Conclusions： The application of phloroglucinol suppressed oxidative stress, inflammation, and overactivity in the bladder. This may provide a new treatment strategy for IC.展开更多
Herein,phloroglucinol tristearate(PhgTS)was used to study the crystallization process due to its unique symmetric structure containing a benzene ring and three aliphatic chains.Spherulites of crystallized PhgTS from f...Herein,phloroglucinol tristearate(PhgTS)was used to study the crystallization process due to its unique symmetric structure containing a benzene ring and three aliphatic chains.Spherulites of crystallized PhgTS from four solvents under diverse conditions were analyzed in detail and their formation process was studied.Maltese cross is shown by PhgTS spherulites obtained from aprotic solvents via polarized optical microscopy.In comparison,no Maltese cross can be observed from branch-like crystals formed from protic solvents.Independent on the micro-scaled morphology,lamellae were found to be the basic blocks constructing both PhgTS spherulites and branch-like crystals,which were formed predominantly by stacked PhgTS molecules.Although differential characters of the solvents did not affect the formation of lamellas,the solvents played a crucial role in the formation of self-assembled microscaled morphologies.In particular,the morphologies of spherulites were strongly affected by the concentration of PhgTS solutions,surrounding tempera-ture and evaporation rate of solvents.Generally,a higher concentration of PhgTS led to more homogeneous spherulites,a lower evaporation rate resulted in more compact spherulites,and a higher surounding temperature generated preferentially more ring-banded spherulites of PhgTS.展开更多
目的研究间苯三酚在第一产程宫颈水肿产妇中的应用效果。方法回顾性选取邳州东大医院2024年1月至2025年2月收治的84例第一产程宫颈水肿产妇,依据治疗方案不同分为两组,每组42例。常规组予以阿托品治疗,治疗组予以间苯三酚治疗,对比两组...目的研究间苯三酚在第一产程宫颈水肿产妇中的应用效果。方法回顾性选取邳州东大医院2024年1月至2025年2月收治的84例第一产程宫颈水肿产妇,依据治疗方案不同分为两组,每组42例。常规组予以阿托品治疗,治疗组予以间苯三酚治疗,对比两组临床疗效、宫口扩张情况(宫口扩张速度、宫口全开时间)、产程、产后出血量、新生儿结局(1、5 min Apgar评分、新生儿窒息发生率)。结果治疗组总有效率高于常规组(P<0.05);治疗组宫口扩张速度大于常规组,宫口全开时间短于常规组(P<0.05);治疗组各产程短于常规组,产后2、24 h出血量少于常规组(P<0.05);治疗组新生儿1、5 min Apgar评分高于常规组,新生儿窒息发生率低于常规组(P<0.05)。结论间苯三酚治疗第一产程宫颈水肿的临床疗效确切,能够促进宫口扩张,缩短产程,减少产后出血,改善新生儿结局。展开更多
文摘Two new phloroglucinol glycosides, lysidiciside A (1) and lysidiciside B (2), were isolated from the roots of Lysidice rhodostega Hance. Their structures were determined as 1-[(3-methylbutyryl)phloroglucinol]-β-D- glucopyranoside (1), 1- [(3-methylbutyryl)phloro- glucinol]-β-D-glucopyranosyl-5-O-β-D-glucopyranoside (2) on the basis of chemical and spectral analysis .
基金funding from Al-Ameen College of Pharmacy, Bangalore, India
文摘Objective: Diabetes is a common metabolic disease with several complications in its patients. Often, people living with diabetes develop erectile dysfunction (ED). The primary aim of this work was to investigate the effect of phloroglucinol in diabetes-induced ED in rats. Methods: Male Wistar rats were given 52 mg/kg of streptozotocin, by intraperitoneal injection, to induce diabetes and ED. Subsequently, animals were grouped into three groups: group 1, diabetic control;group 2, low-dose phloroglucinol (150 mg/kg body weight);and group 3, high-dose phloroglucinol (250 mg/kg body weight). A group of six normal rats served as a normal control. The rats were treated with phloroglucinol for six weeks and then were assessed for treatment effects. Sexual behavior, glycosylated hemoglobin Ale (HbAlc) values, serum testosterone, serum nitric oxide (NO), blood pressure and sperm count were measured after the end of treatment. Results: After a 6-week treatment period, the high dose of phloroglucinol significantly decreased HbAlc values in diabetic rats. Rats treated with phloroglucinol had increased serum testosterone, NO and sperm count. Animals treated with 250 mg/kg phloroglucinol performed similar to normal rats in the sexual behavioral study, suggesting the reversal of complications of ED. Conversely, a decrease in the blood pressure in treated groups was observed. Conclusion: The results highlight the protective effect of phloroglucinol in diabetes-induced ED in rats warranting further studies.
基金financially supported by the National Natural Science Foundation of China(No.81673530)Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(No.2017BT01Y036)。
文摘A novel meroterpenoid,euphoractone(1),was isolated from the extracts of the roots of Euphorbia fischeriana Steud.Its structure was determined by spectroscopic methods and X-ray crystallography.1 possesses an unusual ent-abietane-phloroglucinol skeleton.The plausible biosynthetic pathway for 1 was proposed.1 showed inhibitory activities against human lung cancer H23 and H460 cells with the IC_(50)values of 21.07±3.54 and 20.91±4.07 μmol/L.
基金Supported by the National Natural Science Foundation of China(31070291)
文摘To improve the purity of the total phloroglucinols from Dryopteris crassirhizoma extracts, the separation and purification conditions of the total phloroglucinols from Dryopteris crassirhizoma were studied with DM-130 macroporous adsorption resin in this study. Adsorption rate, elution rate and purity of the total phloroglucinols were used as indexes to investigate the adsorption and desorption capacity of the total phloroglucinols with DM-130 macroporous adsorption resin. Through the study, the optimum sample concentration of the total phloroglucinols and maximum sample volume were 1.5 mg·mL^-1 and 7 BV （210 mL）, respectively. The optimum desorption conditions were achieved by using 80% ethanol as desorption solvent at elution flow rate of 1.0 mL·min^-1. The result showed DM-130 macroporous adsorption resin performed effective adsorption and desorption. After purification, the purity of the total phloroglucinols increased by 11.5-fold.
基金This work was financially supported by the National Natural Science Foundation of China(Nos.31570363 and 31770391)Key Research and Development Plan of Yunnan Province–Special Project of Science and Technology in Yunnan Province(2017IB007)+2 种基金Major Biomedical Project of Yunnan Province(2018ZF005)Innovation Team of the Ministry of Education(No.IRT-17R49)the Foundation of State Key Laboratory of Phytochemistry and Plant Resources in West China(P2017-ZZ04 and P2017-KF06),Kunming Institute of Botany,Chinese Academy of Sciences.
文摘Phytochemical investigation of the MeOH extract of twigs and leaves of Baeckea frutescens led to the isolation of seven new polymethylated phloroglucinol meroterpenoids(PPMs),named baeckfrutones M-S(1-7).Their structures and absolute configurations were determined by spectroscopic analyses,chiral-phase HPLC analysis,and electronic circular dichroism(ECD)calculations.PPM 1 is a novel meroterpenoid possessing a 6/6/5/3 tetracyclic skeleton in PPMs,whereas 3 and 4 are the first hydroxytasmanone type phloroglucinol-monoterpene hybrids.(+)-2 and 7 displayed potent antiinflammatory activity with IC50 values of 20.86±0.60 and 36.21±1.18 lL,respectively.
文摘Two new phloroglucinol glycosides,2,6-dihydroxy-3,5-dimethyl-1-isobutyrylbenzene-4-O-beta-D-glucoside and 2,6-dihydroxy-3,5-dimethyl-1-(2-methylbutyryl)benzene-4-O-beta-D-glucoside were isolated from Hypericum japonicum.Their structures were determined by spectroscopic and chemical methods.
文摘Background:Myocardial injury due to ischemia‐reperfusion(IR)is aggravated in diabetes which is associated with oxidative stress.Alleviating oxidative stress via use of antioxidants has been shown to be effective at minimizing myocardial cell death and improving cardiac function.The aim of the present study was to evaluate the cardioprotective effect of phloroglucinol against myocardial reperfusion injury(MRI)in diabetic rats.Methods:Diabetes was induced in female rats with streptozotocin(50 mg/kg).The diabetic rats were orally treated with phloroglucinol(100 and 200 mg/kg daily for 28 days).After treatment the hearts were isolated and mounted on a Langendorff apparatus.The hearts were subjected to 15 minutes of IR to induce myocardial damage.Cardiac functions including heart rate(HR),resting and developed tension,and rate of change of contraction(+dP/dtmax)were recorded.Cardiac injury biomarkers lactate dehydrogenase(LDH)and creatine kinase(CK‐MB)were measured in the heart perfusate.Levels of the antioxidant enzymes reduced glutathione(GSH)and malondialdehyde(MDA)were measured.Hematoxylin and eosin(H&E)staining was also performed.Results:After IR injury,a decrease in HR and+dP/dtmax in hearts from diabetic rat was seen compared to healthy rat hearts,which was reversed by phloroglucinol treatment.Myocardial infarct size,measured by H&E staining,was increased in diabetic rats compared to healthy rats and an increase in the activity of LDH and CK‐MB in the heart perfusate in diabetic rats was decreased by phloroglucinol treatment.An increase in MDA levels and a decrease in levels of antioxidant enzymes were observed in diabetic rats,which was reversed with phloroglucinol treatment.Conclusion:Phloroglucinol treatment has potential therapeutic promise in the treatment of MRI in diabetes.
文摘Background： Phloroglucinol plays an important role in oxidative stress and inflammatory responses. The effects of phloroglucinol have been proven in various disease models. The aim of the present study was to investigate the efficacy and possible mechanisms of phloroglucinol in the treatment of interstitial cystitis （IC）. Methods： Thirty-two female Sprague-Dawley （SD） rats were used in this study. IC was induced by intraperitoneal injection of cyclophosphamide （CYP）. Rats were randomly allocated to one of four groups （n = 8 per group）： A control group, which was injected with saline （75 mg/kg; i.p.） instead of CYP on days 1, 4, and 7; a chronic IC group, which was injected with CYP （75 mg/kg; i.p.） on days l, 4, and 7; a high-dose （30 mg/kg） phloroglucinol-treated group; and a low-dose （15 mg/kg） phloroglucinol-treated group. On day 8, the rats in each group underwent cystometrography （CMG）, and the bladders were examined for evidence of oxidative stress and inflammation. Statistical analysis was performed by analysis of variance （ANOVA） followed by least square difference multiple comparison po.s＇t-hoc test. Results： Histological evaluation showed that bladder inflammation in CYP-treated rats was suppressed by phloroglucinol. CMG revealed that the CYP treatment induced overactive bladder in rats that was reversed by phloroglucinol. Up-regulated tumor necrosis factor-or and interleukin-6 expression in the CYP-treated rats were also suppressed in the phloroglucinol treated rats. CYP treatment significantly increased myeloperoxidase activity as well as the decreased activities ofcatalase of the bladder, which was reversed by treatment with phloroglucinol. Conclusions： The application of phloroglucinol suppressed oxidative stress, inflammation, and overactivity in the bladder. This may provide a new treatment strategy for IC.
基金Y.Y.thanks the China Scholarship Council(CSC)for financial support.K.Z.thanks Georg-August-University of Goettingen for the Anschubfinanzierung(Funding for the Promotion of Young Academics of University of Goettingen)and Fonds der Chemischen Industrie(FCI)for the financial support.The authors declare no conflicts of interest.
文摘Herein,phloroglucinol tristearate(PhgTS)was used to study the crystallization process due to its unique symmetric structure containing a benzene ring and three aliphatic chains.Spherulites of crystallized PhgTS from four solvents under diverse conditions were analyzed in detail and their formation process was studied.Maltese cross is shown by PhgTS spherulites obtained from aprotic solvents via polarized optical microscopy.In comparison,no Maltese cross can be observed from branch-like crystals formed from protic solvents.Independent on the micro-scaled morphology,lamellae were found to be the basic blocks constructing both PhgTS spherulites and branch-like crystals,which were formed predominantly by stacked PhgTS molecules.Although differential characters of the solvents did not affect the formation of lamellas,the solvents played a crucial role in the formation of self-assembled microscaled morphologies.In particular,the morphologies of spherulites were strongly affected by the concentration of PhgTS solutions,surrounding tempera-ture and evaporation rate of solvents.Generally,a higher concentration of PhgTS led to more homogeneous spherulites,a lower evaporation rate resulted in more compact spherulites,and a higher surounding temperature generated preferentially more ring-banded spherulites of PhgTS.
文摘目的研究间苯三酚在第一产程宫颈水肿产妇中的应用效果。方法回顾性选取邳州东大医院2024年1月至2025年2月收治的84例第一产程宫颈水肿产妇,依据治疗方案不同分为两组,每组42例。常规组予以阿托品治疗,治疗组予以间苯三酚治疗,对比两组临床疗效、宫口扩张情况(宫口扩张速度、宫口全开时间)、产程、产后出血量、新生儿结局(1、5 min Apgar评分、新生儿窒息发生率)。结果治疗组总有效率高于常规组(P<0.05);治疗组宫口扩张速度大于常规组,宫口全开时间短于常规组(P<0.05);治疗组各产程短于常规组,产后2、24 h出血量少于常规组(P<0.05);治疗组新生儿1、5 min Apgar评分高于常规组,新生儿窒息发生率低于常规组(P<0.05)。结论间苯三酚治疗第一产程宫颈水肿的临床疗效确切,能够促进宫口扩张,缩短产程,减少产后出血,改善新生儿结局。
文摘目的 探讨间苯三酚联合利托君治疗高龄初产妇先兆早产的临床疗效。方法 选取2022年1月至2024年12月河池市人民医院收治的60例先兆早产高龄初产妇,按随机数字表法分为对照组与观察组,各30例。对照组予以利托君治疗,观察组予以间苯三酚联合利托君治疗。比较两组宫缩消失时间、早产率、分娩孕期、孕期延长时间、新生儿出生后1 min Apgar评分、新生儿体重、不良反应发生率、血管化血流指数(VFI)、血流指数(FI)及临床疗效。结果 观察组比对照组宫缩消失时间缩短、早产率降低(P<0.05),孕期延长时间及分娩孕期增大(P<0.05)。观察组新生儿不良结局发生率低于对照组(P<0.05),出生后1 min Apgar评分及体重高于对照组(P<0.05)。两组不良反应发生率比较差异无统计学意义(P>0.05)。治疗前两组VFI、FI比较无统计学差异(P>0.05);治疗后,观察组VFI、FI较对照组降低(P<0.05)。观察组治疗有效率高于对照组(P<0.05)。结论 间苯三酚联合利托君治疗先兆早产安全有效,可延长高龄初产妇妊娠时间,稳定胎盘血流灌注,改善母婴结局。
