Two new phloroglucinol glycosides, lysidiciside A (1) and lysidiciside B (2), were isolated from the roots of Lysidice rhodostega Hance. Their structures were determined as 1-[(3-methylbutyryl)phloroglucinol]-β-D- g...Two new phloroglucinol glycosides, lysidiciside A (1) and lysidiciside B (2), were isolated from the roots of Lysidice rhodostega Hance. Their structures were determined as 1-[(3-methylbutyryl)phloroglucinol]-β-D- glucopyranoside (1), 1- [(3-methylbutyryl)phloro- glucinol]-β-D-glucopyranosyl-5-O-β-D-glucopyranoside (2) on the basis of chemical and spectral analysis .展开更多
Objective: Diabetes is a common metabolic disease with several complications in its patients. Often, people living with diabetes develop erectile dysfunction (ED). The primary aim of this work was to investigate the e...Objective: Diabetes is a common metabolic disease with several complications in its patients. Often, people living with diabetes develop erectile dysfunction (ED). The primary aim of this work was to investigate the effect of phloroglucinol in diabetes-induced ED in rats. Methods: Male Wistar rats were given 52 mg/kg of streptozotocin, by intraperitoneal injection, to induce diabetes and ED. Subsequently, animals were grouped into three groups: group 1, diabetic control;group 2, low-dose phloroglucinol (150 mg/kg body weight);and group 3, high-dose phloroglucinol (250 mg/kg body weight). A group of six normal rats served as a normal control. The rats were treated with phloroglucinol for six weeks and then were assessed for treatment effects. Sexual behavior, glycosylated hemoglobin Ale (HbAlc) values, serum testosterone, serum nitric oxide (NO), blood pressure and sperm count were measured after the end of treatment. Results: After a 6-week treatment period, the high dose of phloroglucinol significantly decreased HbAlc values in diabetic rats. Rats treated with phloroglucinol had increased serum testosterone, NO and sperm count. Animals treated with 250 mg/kg phloroglucinol performed similar to normal rats in the sexual behavioral study, suggesting the reversal of complications of ED. Conversely, a decrease in the blood pressure in treated groups was observed. Conclusion: The results highlight the protective effect of phloroglucinol in diabetes-induced ED in rats warranting further studies.展开更多
A novel meroterpenoid,euphoractone(1),was isolated from the extracts of the roots of Euphorbia fischeriana Steud.Its structure was determined by spectroscopic methods and X-ray crystallography.1 possesses an unusual e...A novel meroterpenoid,euphoractone(1),was isolated from the extracts of the roots of Euphorbia fischeriana Steud.Its structure was determined by spectroscopic methods and X-ray crystallography.1 possesses an unusual ent-abietane-phloroglucinol skeleton.The plausible biosynthetic pathway for 1 was proposed.1 showed inhibitory activities against human lung cancer H23 and H460 cells with the IC_(50)values of 21.07±3.54 and 20.91±4.07 μmol/L.展开更多
To improve the purity of the total phloroglucinols from Dryopteris crassirhizoma extracts, the separation and purification conditions of the total phloroglucinols from Dryopteris crassirhizoma were studied with DM-130...To improve the purity of the total phloroglucinols from Dryopteris crassirhizoma extracts, the separation and purification conditions of the total phloroglucinols from Dryopteris crassirhizoma were studied with DM-130 macroporous adsorption resin in this study. Adsorption rate, elution rate and purity of the total phloroglucinols were used as indexes to investigate the adsorption and desorption capacity of the total phloroglucinols with DM-130 macroporous adsorption resin. Through the study, the optimum sample concentration of the total phloroglucinols and maximum sample volume were 1.5 mg·mL^-1 and 7 BV （210 mL）, respectively. The optimum desorption conditions were achieved by using 80% ethanol as desorption solvent at elution flow rate of 1.0 mL·min^-1. The result showed DM-130 macroporous adsorption resin performed effective adsorption and desorption. After purification, the purity of the total phloroglucinols increased by 11.5-fold.展开更多
Phytochemical investigation of the MeOH extract of twigs and leaves of Baeckea frutescens led to the isolation of seven new polymethylated phloroglucinol meroterpenoids(PPMs),named baeckfrutones M-S(1-7).Their structu...Phytochemical investigation of the MeOH extract of twigs and leaves of Baeckea frutescens led to the isolation of seven new polymethylated phloroglucinol meroterpenoids(PPMs),named baeckfrutones M-S(1-7).Their structures and absolute configurations were determined by spectroscopic analyses,chiral-phase HPLC analysis,and electronic circular dichroism(ECD)calculations.PPM 1 is a novel meroterpenoid possessing a 6/6/5/3 tetracyclic skeleton in PPMs,whereas 3 and 4 are the first hydroxytasmanone type phloroglucinol-monoterpene hybrids.(+)-2 and 7 displayed potent antiinflammatory activity with IC50 values of 20.86±0.60 and 36.21±1.18 lL,respectively.展开更多
Background:Myocardial injury due to ischemia‐reperfusion(IR)is aggravated in diabetes which is associated with oxidative stress.Alleviating oxidative stress via use of antioxidants has been shown to be effective at m...Background:Myocardial injury due to ischemia‐reperfusion(IR)is aggravated in diabetes which is associated with oxidative stress.Alleviating oxidative stress via use of antioxidants has been shown to be effective at minimizing myocardial cell death and improving cardiac function.The aim of the present study was to evaluate the cardioprotective effect of phloroglucinol against myocardial reperfusion injury(MRI)in diabetic rats.Methods:Diabetes was induced in female rats with streptozotocin(50 mg/kg).The diabetic rats were orally treated with phloroglucinol(100 and 200 mg/kg daily for 28 days).After treatment the hearts were isolated and mounted on a Langendorff apparatus.The hearts were subjected to 15 minutes of IR to induce myocardial damage.Cardiac functions including heart rate(HR),resting and developed tension,and rate of change of contraction(+dP/dtmax)were recorded.Cardiac injury biomarkers lactate dehydrogenase(LDH)and creatine kinase(CK‐MB)were measured in the heart perfusate.Levels of the antioxidant enzymes reduced glutathione(GSH)and malondialdehyde(MDA)were measured.Hematoxylin and eosin(H&E)staining was also performed.Results:After IR injury,a decrease in HR and+dP/dtmax in hearts from diabetic rat was seen compared to healthy rat hearts,which was reversed by phloroglucinol treatment.Myocardial infarct size,measured by H&E staining,was increased in diabetic rats compared to healthy rats and an increase in the activity of LDH and CK‐MB in the heart perfusate in diabetic rats was decreased by phloroglucinol treatment.An increase in MDA levels and a decrease in levels of antioxidant enzymes were observed in diabetic rats,which was reversed with phloroglucinol treatment.Conclusion:Phloroglucinol treatment has potential therapeutic promise in the treatment of MRI in diabetes.展开更多
Two new phloroglucinol glycosides, 2,6-dihydroxy-3,5-dimethyl-1-isobutyrylbenzene-4-O-beta-D-glucoside and 2,6-dihydroxy-3,5-dimethyl-1-(2-methylbutyryl) benzene-4-O-beta-D-glucoside were isolated from Hypericum japon...Two new phloroglucinol glycosides, 2,6-dihydroxy-3,5-dimethyl-1-isobutyrylbenzene-4-O-beta-D-glucoside and 2,6-dihydroxy-3,5-dimethyl-1-(2-methylbutyryl) benzene-4-O-beta-D-glucoside were isolated from Hypericum japonicum. Their structures were determined by spectroscopic and chemical methods.展开更多
目的以注射用间苯三酚、注射用法莫替丁和盐酸雷尼替丁注射液为代表考察三种消化科常用注射剂与复方电解质醋酸钠葡萄糖注射液的配伍稳定性。方法注射用间苯三酚、注射用法莫替丁和盐酸雷尼替丁注射液与复方电解质醋酸钠葡萄糖注射液配...目的以注射用间苯三酚、注射用法莫替丁和盐酸雷尼替丁注射液为代表考察三种消化科常用注射剂与复方电解质醋酸钠葡萄糖注射液的配伍稳定性。方法注射用间苯三酚、注射用法莫替丁和盐酸雷尼替丁注射液与复方电解质醋酸钠葡萄糖注射液配伍后,于0、1、2、4、8 h检测配伍溶液的澄明度、pH值、电导率、不溶性微粒(用不溶性微粒检测仪测定≥10μm、≥25μm的微粒数)和各自主药成分含量的变化。结果注射用间苯三酚、注射用法莫替丁和盐酸雷尼替丁注射液配伍结果均表明三种配伍溶液在室温放置8 h内未出现变色、气泡、沉淀、浑浊等明显外观变化;注射用间苯三酚配伍液在配伍后0~8 h pH值、电导率的相对标准差(RSD)分别为0.14%、0.66%,注射用法莫替丁配伍液为0.13%、2.35%,盐酸雷尼替丁注射液配伍液为0.12%、2.00%;三者不溶性微粒均在中国药典规定范围内;三种配伍液中,间苯三酚、法莫替丁、雷尼替丁含量0~8 h RSD分别为1.29%、0.58%、4.14%。结论注射用间苯三酚、注射用法莫替丁和盐酸雷尼替丁注射液与复方电解质醋酸钠葡萄糖注射液配伍8 h内稳定。展开更多
Background： Phloroglucinol plays an important role in oxidative stress and inflammatory responses. The effects of phloroglucinol have been proven in various disease models. The aim of the present study was to investi...Background： Phloroglucinol plays an important role in oxidative stress and inflammatory responses. The effects of phloroglucinol have been proven in various disease models. The aim of the present study was to investigate the efficacy and possible mechanisms of phloroglucinol in the treatment of interstitial cystitis （IC）. Methods： Thirty-two female Sprague-Dawley （SD） rats were used in this study. IC was induced by intraperitoneal injection of cyclophosphamide （CYP）. Rats were randomly allocated to one of four groups （n = 8 per group）： A control group, which was injected with saline （75 mg/kg; i.p.） instead of CYP on days 1, 4, and 7; a chronic IC group, which was injected with CYP （75 mg/kg; i.p.） on days l, 4, and 7; a high-dose （30 mg/kg） phloroglucinol-treated group; and a low-dose （15 mg/kg） phloroglucinol-treated group. On day 8, the rats in each group underwent cystometrography （CMG）, and the bladders were examined for evidence of oxidative stress and inflammation. Statistical analysis was performed by analysis of variance （ANOVA） followed by least square difference multiple comparison po.s＇t-hoc test. Results： Histological evaluation showed that bladder inflammation in CYP-treated rats was suppressed by phloroglucinol. CMG revealed that the CYP treatment induced overactive bladder in rats that was reversed by phloroglucinol. Up-regulated tumor necrosis factor-or and interleukin-6 expression in the CYP-treated rats were also suppressed in the phloroglucinol treated rats. CYP treatment significantly increased myeloperoxidase activity as well as the decreased activities ofcatalase of the bladder, which was reversed by treatment with phloroglucinol. Conclusions： The application of phloroglucinol suppressed oxidative stress, inflammation, and overactivity in the bladder. This may provide a new treatment strategy for IC.展开更多
目的:探讨间苯三酚联合缩宫素在经阴道分娩产妇中的应用。方法:选取2021年1月—2023年12月北京市朝阳区妇幼保健院收治的82例经阴道分娩产妇,按照随机数表法分为两组,其中对照组(n=41)采用缩宫素治疗,观察组(n=41)联合间苯三酚治疗。比...目的:探讨间苯三酚联合缩宫素在经阴道分娩产妇中的应用。方法:选取2021年1月—2023年12月北京市朝阳区妇幼保健院收治的82例经阴道分娩产妇,按照随机数表法分为两组,其中对照组(n=41)采用缩宫素治疗,观察组(n=41)联合间苯三酚治疗。比较两组产程进展、产妇宫颈评分、新生儿出生后1 min Apgar评分、围产期总出血量及并发症发生率。结果:观察组总产程及各产程时间均短于对照组,差异有统计学意义(P<0.05)。治疗后,观察组Bishop评分、新生儿出生后1 min Apgar评分均较对照组更高,而围产期总出血量更少,差异有统计学意义(P<0.05)。观察组并发症发生率为7.32%,低于对照组的24.39%,差异有统计学意义(P<0.05)。结论:采用间苯三酚联合缩宫素可加速阴道分娩产妇产程进展,促进宫颈扩张,且对母体及新生儿结局无显著不良影响,安全性良好。展开更多
文摘Two new phloroglucinol glycosides, lysidiciside A (1) and lysidiciside B (2), were isolated from the roots of Lysidice rhodostega Hance. Their structures were determined as 1-[(3-methylbutyryl)phloroglucinol]-β-D- glucopyranoside (1), 1- [(3-methylbutyryl)phloro- glucinol]-β-D-glucopyranosyl-5-O-β-D-glucopyranoside (2) on the basis of chemical and spectral analysis .
基金funding from Al-Ameen College of Pharmacy, Bangalore, India
文摘Objective: Diabetes is a common metabolic disease with several complications in its patients. Often, people living with diabetes develop erectile dysfunction (ED). The primary aim of this work was to investigate the effect of phloroglucinol in diabetes-induced ED in rats. Methods: Male Wistar rats were given 52 mg/kg of streptozotocin, by intraperitoneal injection, to induce diabetes and ED. Subsequently, animals were grouped into three groups: group 1, diabetic control;group 2, low-dose phloroglucinol (150 mg/kg body weight);and group 3, high-dose phloroglucinol (250 mg/kg body weight). A group of six normal rats served as a normal control. The rats were treated with phloroglucinol for six weeks and then were assessed for treatment effects. Sexual behavior, glycosylated hemoglobin Ale (HbAlc) values, serum testosterone, serum nitric oxide (NO), blood pressure and sperm count were measured after the end of treatment. Results: After a 6-week treatment period, the high dose of phloroglucinol significantly decreased HbAlc values in diabetic rats. Rats treated with phloroglucinol had increased serum testosterone, NO and sperm count. Animals treated with 250 mg/kg phloroglucinol performed similar to normal rats in the sexual behavioral study, suggesting the reversal of complications of ED. Conversely, a decrease in the blood pressure in treated groups was observed. Conclusion: The results highlight the protective effect of phloroglucinol in diabetes-induced ED in rats warranting further studies.
基金financially supported by the National Natural Science Foundation of China(No.81673530)Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(No.2017BT01Y036)。
文摘A novel meroterpenoid,euphoractone(1),was isolated from the extracts of the roots of Euphorbia fischeriana Steud.Its structure was determined by spectroscopic methods and X-ray crystallography.1 possesses an unusual ent-abietane-phloroglucinol skeleton.The plausible biosynthetic pathway for 1 was proposed.1 showed inhibitory activities against human lung cancer H23 and H460 cells with the IC_(50)values of 21.07±3.54 and 20.91±4.07 μmol/L.
基金Supported by the National Natural Science Foundation of China(31070291)
文摘To improve the purity of the total phloroglucinols from Dryopteris crassirhizoma extracts, the separation and purification conditions of the total phloroglucinols from Dryopteris crassirhizoma were studied with DM-130 macroporous adsorption resin in this study. Adsorption rate, elution rate and purity of the total phloroglucinols were used as indexes to investigate the adsorption and desorption capacity of the total phloroglucinols with DM-130 macroporous adsorption resin. Through the study, the optimum sample concentration of the total phloroglucinols and maximum sample volume were 1.5 mg·mL^-1 and 7 BV （210 mL）, respectively. The optimum desorption conditions were achieved by using 80% ethanol as desorption solvent at elution flow rate of 1.0 mL·min^-1. The result showed DM-130 macroporous adsorption resin performed effective adsorption and desorption. After purification, the purity of the total phloroglucinols increased by 11.5-fold.
基金This work was financially supported by the National Natural Science Foundation of China(Nos.31570363 and 31770391)Key Research and Development Plan of Yunnan Province–Special Project of Science and Technology in Yunnan Province(2017IB007)+2 种基金Major Biomedical Project of Yunnan Province(2018ZF005)Innovation Team of the Ministry of Education(No.IRT-17R49)the Foundation of State Key Laboratory of Phytochemistry and Plant Resources in West China(P2017-ZZ04 and P2017-KF06),Kunming Institute of Botany,Chinese Academy of Sciences.
文摘Phytochemical investigation of the MeOH extract of twigs and leaves of Baeckea frutescens led to the isolation of seven new polymethylated phloroglucinol meroterpenoids(PPMs),named baeckfrutones M-S(1-7).Their structures and absolute configurations were determined by spectroscopic analyses,chiral-phase HPLC analysis,and electronic circular dichroism(ECD)calculations.PPM 1 is a novel meroterpenoid possessing a 6/6/5/3 tetracyclic skeleton in PPMs,whereas 3 and 4 are the first hydroxytasmanone type phloroglucinol-monoterpene hybrids.(+)-2 and 7 displayed potent antiinflammatory activity with IC50 values of 20.86±0.60 and 36.21±1.18 lL,respectively.
文摘Background:Myocardial injury due to ischemia‐reperfusion(IR)is aggravated in diabetes which is associated with oxidative stress.Alleviating oxidative stress via use of antioxidants has been shown to be effective at minimizing myocardial cell death and improving cardiac function.The aim of the present study was to evaluate the cardioprotective effect of phloroglucinol against myocardial reperfusion injury(MRI)in diabetic rats.Methods:Diabetes was induced in female rats with streptozotocin(50 mg/kg).The diabetic rats were orally treated with phloroglucinol(100 and 200 mg/kg daily for 28 days).After treatment the hearts were isolated and mounted on a Langendorff apparatus.The hearts were subjected to 15 minutes of IR to induce myocardial damage.Cardiac functions including heart rate(HR),resting and developed tension,and rate of change of contraction(+dP/dtmax)were recorded.Cardiac injury biomarkers lactate dehydrogenase(LDH)and creatine kinase(CK‐MB)were measured in the heart perfusate.Levels of the antioxidant enzymes reduced glutathione(GSH)and malondialdehyde(MDA)were measured.Hematoxylin and eosin(H&E)staining was also performed.Results:After IR injury,a decrease in HR and+dP/dtmax in hearts from diabetic rat was seen compared to healthy rat hearts,which was reversed by phloroglucinol treatment.Myocardial infarct size,measured by H&E staining,was increased in diabetic rats compared to healthy rats and an increase in the activity of LDH and CK‐MB in the heart perfusate in diabetic rats was decreased by phloroglucinol treatment.An increase in MDA levels and a decrease in levels of antioxidant enzymes were observed in diabetic rats,which was reversed with phloroglucinol treatment.Conclusion:Phloroglucinol treatment has potential therapeutic promise in the treatment of MRI in diabetes.
文摘Two new phloroglucinol glycosides, 2,6-dihydroxy-3,5-dimethyl-1-isobutyrylbenzene-4-O-beta-D-glucoside and 2,6-dihydroxy-3,5-dimethyl-1-(2-methylbutyryl) benzene-4-O-beta-D-glucoside were isolated from Hypericum japonicum. Their structures were determined by spectroscopic and chemical methods.
文摘目的 探讨间苯三酚联合利托君治疗高龄初产妇先兆早产的临床疗效。方法 选取2022年1月至2024年12月河池市人民医院收治的60例先兆早产高龄初产妇,按随机数字表法分为对照组与观察组,各30例。对照组予以利托君治疗,观察组予以间苯三酚联合利托君治疗。比较两组宫缩消失时间、早产率、分娩孕期、孕期延长时间、新生儿出生后1 min Apgar评分、新生儿体重、不良反应发生率、血管化血流指数(VFI)、血流指数(FI)及临床疗效。结果 观察组比对照组宫缩消失时间缩短、早产率降低(P<0.05),孕期延长时间及分娩孕期增大(P<0.05)。观察组新生儿不良结局发生率低于对照组(P<0.05),出生后1 min Apgar评分及体重高于对照组(P<0.05)。两组不良反应发生率比较差异无统计学意义(P>0.05)。治疗前两组VFI、FI比较无统计学差异(P>0.05);治疗后,观察组VFI、FI较对照组降低(P<0.05)。观察组治疗有效率高于对照组(P<0.05)。结论 间苯三酚联合利托君治疗先兆早产安全有效,可延长高龄初产妇妊娠时间,稳定胎盘血流灌注,改善母婴结局。
文摘目的以注射用间苯三酚、注射用法莫替丁和盐酸雷尼替丁注射液为代表考察三种消化科常用注射剂与复方电解质醋酸钠葡萄糖注射液的配伍稳定性。方法注射用间苯三酚、注射用法莫替丁和盐酸雷尼替丁注射液与复方电解质醋酸钠葡萄糖注射液配伍后,于0、1、2、4、8 h检测配伍溶液的澄明度、pH值、电导率、不溶性微粒(用不溶性微粒检测仪测定≥10μm、≥25μm的微粒数)和各自主药成分含量的变化。结果注射用间苯三酚、注射用法莫替丁和盐酸雷尼替丁注射液配伍结果均表明三种配伍溶液在室温放置8 h内未出现变色、气泡、沉淀、浑浊等明显外观变化;注射用间苯三酚配伍液在配伍后0~8 h pH值、电导率的相对标准差(RSD)分别为0.14%、0.66%,注射用法莫替丁配伍液为0.13%、2.35%,盐酸雷尼替丁注射液配伍液为0.12%、2.00%;三者不溶性微粒均在中国药典规定范围内;三种配伍液中,间苯三酚、法莫替丁、雷尼替丁含量0~8 h RSD分别为1.29%、0.58%、4.14%。结论注射用间苯三酚、注射用法莫替丁和盐酸雷尼替丁注射液与复方电解质醋酸钠葡萄糖注射液配伍8 h内稳定。
文摘Background： Phloroglucinol plays an important role in oxidative stress and inflammatory responses. The effects of phloroglucinol have been proven in various disease models. The aim of the present study was to investigate the efficacy and possible mechanisms of phloroglucinol in the treatment of interstitial cystitis （IC）. Methods： Thirty-two female Sprague-Dawley （SD） rats were used in this study. IC was induced by intraperitoneal injection of cyclophosphamide （CYP）. Rats were randomly allocated to one of four groups （n = 8 per group）： A control group, which was injected with saline （75 mg/kg; i.p.） instead of CYP on days 1, 4, and 7; a chronic IC group, which was injected with CYP （75 mg/kg; i.p.） on days l, 4, and 7; a high-dose （30 mg/kg） phloroglucinol-treated group; and a low-dose （15 mg/kg） phloroglucinol-treated group. On day 8, the rats in each group underwent cystometrography （CMG）, and the bladders were examined for evidence of oxidative stress and inflammation. Statistical analysis was performed by analysis of variance （ANOVA） followed by least square difference multiple comparison po.s＇t-hoc test. Results： Histological evaluation showed that bladder inflammation in CYP-treated rats was suppressed by phloroglucinol. CMG revealed that the CYP treatment induced overactive bladder in rats that was reversed by phloroglucinol. Up-regulated tumor necrosis factor-or and interleukin-6 expression in the CYP-treated rats were also suppressed in the phloroglucinol treated rats. CYP treatment significantly increased myeloperoxidase activity as well as the decreased activities ofcatalase of the bladder, which was reversed by treatment with phloroglucinol. Conclusions： The application of phloroglucinol suppressed oxidative stress, inflammation, and overactivity in the bladder. This may provide a new treatment strategy for IC.
文摘目的:探讨间苯三酚联合缩宫素在经阴道分娩产妇中的应用。方法:选取2021年1月—2023年12月北京市朝阳区妇幼保健院收治的82例经阴道分娩产妇,按照随机数表法分为两组,其中对照组(n=41)采用缩宫素治疗,观察组(n=41)联合间苯三酚治疗。比较两组产程进展、产妇宫颈评分、新生儿出生后1 min Apgar评分、围产期总出血量及并发症发生率。结果:观察组总产程及各产程时间均短于对照组,差异有统计学意义(P<0.05)。治疗后,观察组Bishop评分、新生儿出生后1 min Apgar评分均较对照组更高,而围产期总出血量更少,差异有统计学意义(P<0.05)。观察组并发症发生率为7.32%,低于对照组的24.39%,差异有统计学意义(P<0.05)。结论:采用间苯三酚联合缩宫素可加速阴道分娩产妇产程进展,促进宫颈扩张,且对母体及新生儿结局无显著不良影响,安全性良好。
