目的基于基因芯片数据分析PHD锌指蛋白19(PHD finger protein 19,PHF19)在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达及预后价值。方法下载癌症基因组图谱(The Cancer Genome Atlas,TCGA)肺癌患者数据并分析PHF19在肺癌...目的基于基因芯片数据分析PHD锌指蛋白19(PHD finger protein 19,PHF19)在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达及预后价值。方法下载癌症基因组图谱(The Cancer Genome Atlas,TCGA)肺癌患者数据并分析PHF19在肺癌中表达,通过基因表达数据库(Gene Expression Omnibus,GEO)下载数据集GSE30219,GSE50081筛选患者分别纳入训练集和验证集,并分析PHF19表达与肺癌患者性别、年龄、肿瘤临床分期、病理类型与无病生存率(disease free survival,DFS)、总生存率(overall survival,OS)的关系,通过在线数据库对肺癌患者PHF19及共表达相关基因进行基因本体论与京都基因和基因组百科全书(Gene Ontology and Kyoto Encyclopedia of Genes and Genomes,GO-KEGG)富集分析、免疫浸润分析。结果TCGA、GEO数据显示PHF19在肺癌中高表达(P<0.001),PHF19表达与肿瘤分期相关。PHF19低表达组的NSCLC患者较高表达组具有更长的DFS及OS(P均<0.05)。多因素COX回归分析显示PHF19为NSCLC患者独立预后影响因素(P<0.05)。绘制列线图预测肺癌患者的生存率并验证C指数表明模型准确度良好。基因富集分析显示PHF19高表达主要与细胞周期、细胞核、染色质等相关。免疫浸润分析显示PHF19与免疫细胞浸润密切相关。结论PHF19可作为预测NSCLC预后的指标,PHF19高表达是NSCLC不良预后的独立预测因子,可能是其治疗的新的靶点。展开更多
目的:探讨多发性骨髓瘤(MM)患者PHF19基因表达水平及其临床意义。方法:通过实时荧光定量PCR方法检测69例初诊MM患者、20例难治复发MM患者及正常对照PHF19基因表达,分析其表达水平与临床特征、疗效及预后之间的关系。结果:初诊患者PHF19...目的:探讨多发性骨髓瘤(MM)患者PHF19基因表达水平及其临床意义。方法:通过实时荧光定量PCR方法检测69例初诊MM患者、20例难治复发MM患者及正常对照PHF19基因表达,分析其表达水平与临床特征、疗效及预后之间的关系。结果:初诊患者PHF19水平明显高于正常对照(0.0206 vs 0.0008,P=0.000);相比于初诊患者,难治复发患者PHF19水平显著升高(0.0206 vs 0.0798,P=0.022)。在初诊患者中,以PHF19基因表达水平中位数作为分界值,分为低表达与高表达组,PHF19基因表达水平与患者性别、年龄、免疫球蛋白亚型及疾病负荷无相关;不同DS、ISS分期患者PHF19基因表达水平无统计学差异(P>0.05);中位随访22个月,PHF19基因低表达组OS率及PFS率优于高表达组。结论:PHF19基因表达与患者疗效和预后存在一定相关性,PHF19高表达患者预后不良。展开更多
Objective:To investigate the expression level,clinical prognosis,genetic changes,methylation value,biological function and immunomodulatory effects of PHF19 in lung adenocarcinoma.Methods:Based on the RNA cohort data ...Objective:To investigate the expression level,clinical prognosis,genetic changes,methylation value,biological function and immunomodulatory effects of PHF19 in lung adenocarcinoma.Methods:Based on the RNA cohort data of lung adenocarcinoma in the Cancer Genome Atlas(TCGA)database,the expression difference of PHF19 between lung adenocarcinoma and normal tissues and the relationship between the expression level and the prognosis of patients were analyzed by using TIMER database and UALCAN database.The gene mutation of PHF19 in lung adenocarcinoma was analyzed in cBioPortal database and TIMER database.The promoter methylation level of PHF19 in lung adenocarcinoma was analyzed in UALCAN database.The enrichment of PHF19 co-expression gene was analyzed in LinkedOmics database and KEGG database.The correlation between the expression of PHF19 in lung adenocarcinoma and the level of immunoinfiltration was explored in the TIMER database.Results:The expression of PHF19 in lung adenocarcinoma tissues was up-regulated,and its high expression was significantly correlated with the pathological stage,lymph node metastasis and shortened overall survival of lung adenocarcinoma patients.Genetic variation was found in 0.9%of patients,amplification and missense mutation were the most common mutations,and TP53 mutation could lead to the high expression of PHF19.The promoter methylation level of PHF19 significantly decreased in lung adenocarcinoma.The function of PHF19 was focused on the process of cell mitosis and cell cycle.The expression level of PHF19 was significantly correlated with tumor immune cell infiltration and immune checkpoint expression.Conclusion:PHF19 can be used as a marker of poor prognosis of lung adenocarcinoma and plays an important role in the occurrence and development of lung adenocarcinoma.展开更多
文摘目的基于基因芯片数据分析PHD锌指蛋白19(PHD finger protein 19,PHF19)在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达及预后价值。方法下载癌症基因组图谱(The Cancer Genome Atlas,TCGA)肺癌患者数据并分析PHF19在肺癌中表达,通过基因表达数据库(Gene Expression Omnibus,GEO)下载数据集GSE30219,GSE50081筛选患者分别纳入训练集和验证集,并分析PHF19表达与肺癌患者性别、年龄、肿瘤临床分期、病理类型与无病生存率(disease free survival,DFS)、总生存率(overall survival,OS)的关系,通过在线数据库对肺癌患者PHF19及共表达相关基因进行基因本体论与京都基因和基因组百科全书(Gene Ontology and Kyoto Encyclopedia of Genes and Genomes,GO-KEGG)富集分析、免疫浸润分析。结果TCGA、GEO数据显示PHF19在肺癌中高表达(P<0.001),PHF19表达与肿瘤分期相关。PHF19低表达组的NSCLC患者较高表达组具有更长的DFS及OS(P均<0.05)。多因素COX回归分析显示PHF19为NSCLC患者独立预后影响因素(P<0.05)。绘制列线图预测肺癌患者的生存率并验证C指数表明模型准确度良好。基因富集分析显示PHF19高表达主要与细胞周期、细胞核、染色质等相关。免疫浸润分析显示PHF19与免疫细胞浸润密切相关。结论PHF19可作为预测NSCLC预后的指标,PHF19高表达是NSCLC不良预后的独立预测因子,可能是其治疗的新的靶点。
文摘目的:探讨多发性骨髓瘤(MM)患者PHF19基因表达水平及其临床意义。方法:通过实时荧光定量PCR方法检测69例初诊MM患者、20例难治复发MM患者及正常对照PHF19基因表达,分析其表达水平与临床特征、疗效及预后之间的关系。结果:初诊患者PHF19水平明显高于正常对照(0.0206 vs 0.0008,P=0.000);相比于初诊患者,难治复发患者PHF19水平显著升高(0.0206 vs 0.0798,P=0.022)。在初诊患者中,以PHF19基因表达水平中位数作为分界值,分为低表达与高表达组,PHF19基因表达水平与患者性别、年龄、免疫球蛋白亚型及疾病负荷无相关;不同DS、ISS分期患者PHF19基因表达水平无统计学差异(P>0.05);中位随访22个月,PHF19基因低表达组OS率及PFS率优于高表达组。结论:PHF19基因表达与患者疗效和预后存在一定相关性,PHF19高表达患者预后不良。
基金Natural Science Foundation of Shanxi Province(201901D111391)。
文摘Objective:To investigate the expression level,clinical prognosis,genetic changes,methylation value,biological function and immunomodulatory effects of PHF19 in lung adenocarcinoma.Methods:Based on the RNA cohort data of lung adenocarcinoma in the Cancer Genome Atlas(TCGA)database,the expression difference of PHF19 between lung adenocarcinoma and normal tissues and the relationship between the expression level and the prognosis of patients were analyzed by using TIMER database and UALCAN database.The gene mutation of PHF19 in lung adenocarcinoma was analyzed in cBioPortal database and TIMER database.The promoter methylation level of PHF19 in lung adenocarcinoma was analyzed in UALCAN database.The enrichment of PHF19 co-expression gene was analyzed in LinkedOmics database and KEGG database.The correlation between the expression of PHF19 in lung adenocarcinoma and the level of immunoinfiltration was explored in the TIMER database.Results:The expression of PHF19 in lung adenocarcinoma tissues was up-regulated,and its high expression was significantly correlated with the pathological stage,lymph node metastasis and shortened overall survival of lung adenocarcinoma patients.Genetic variation was found in 0.9%of patients,amplification and missense mutation were the most common mutations,and TP53 mutation could lead to the high expression of PHF19.The promoter methylation level of PHF19 significantly decreased in lung adenocarcinoma.The function of PHF19 was focused on the process of cell mitosis and cell cycle.The expression level of PHF19 was significantly correlated with tumor immune cell infiltration and immune checkpoint expression.Conclusion:PHF19 can be used as a marker of poor prognosis of lung adenocarcinoma and plays an important role in the occurrence and development of lung adenocarcinoma.
