Grating-based X-ray phase-contrast imaging enhances the contrast of imaged objects,particularly soft tissues.However,the radiation dose in computed tomography(CT)is generally excessive owing to the complex collection ...Grating-based X-ray phase-contrast imaging enhances the contrast of imaged objects,particularly soft tissues.However,the radiation dose in computed tomography(CT)is generally excessive owing to the complex collection scheme.Sparse-view CT collection reduces the radiation dose,but with reduced resolution and reconstructed artifacts particularly in analytical reconstruction methods.Recently,deep learning has been employed in sparse-view CT reconstruction and achieved stateof-the-art results.Nevertheless,its low generalization performance and requirement for abundant training datasets have hindered the practical application of deep learning in phase-contrast CT.In this study,a CT model was used to generate a substantial number of simulated training datasets,thereby circumventing the need for experimental datasets.By training a network with simulated training datasets,the proposed method achieves high generalization performance in attenuationbased CT and phase-contrast CT,despite the lack of sufficient experimental datasets.In experiments utilizing only half of the CT data,our proposed method obtained an image quality comparable to that of the filtered back-projection algorithm with full-view projection.The proposed method simultaneously addresses two challenges in phase-contrast three-dimensional imaging,namely the lack of experimental datasets and the high exposure dose,through model-driven deep learning.This method significantly accelerates the practical application of phase-contrast CT.展开更多
The optimal velocity encoding of phase-contrast magnetic resonance angiography （PC MRA） in measuring cerebral blood flow volume （BFV） ranges from 60 to 80 cm/s. To verify the accuracy of two-dimensional （2D） PC ...The optimal velocity encoding of phase-contrast magnetic resonance angiography （PC MRA） in measuring cerebral blood flow volume （BFV） ranges from 60 to 80 cm/s. To verify the accuracy of two-dimensional （2D） PC MRA, the present study localized the region of interest at blood vessels of the neck using PC MRA based on three-dimensional time-of-flight sequences, and the velocity encoding was set to 80 cm/s. Results of the measurements showed that the error rate was 7.0±6.0% in the estimation of BFV in the internal carotid artery, the external carotid artery and the ipsilateral common carotid artery. There was no significant difference, and a significant correlation in BFV between internal carotid artery ＋ external carotid artery and ipsilateral common carotid artery. In addition, the BFV of the common carotid artery was correlated with that of the ipsilateral internal carotid artery. The main error was attributed to the external carotid artery and its branches. Therefore, after selecting the appropriate scanning parameters and protocols, 2D PC MRA is more accurate in the determination of BFV in the carotid arteries.展开更多
This paper gives theoretical analysis of visibility of fringes, which is influenced by distances, temporal and spatial coherence of source, in hard x-ray differential phase-contrast imaging with microfocus x-ray sourc...This paper gives theoretical analysis of visibility of fringes, which is influenced by distances, temporal and spatial coherence of source, in hard x-ray differential phase-contrast imaging with microfocus x-ray source. According to the character of longitudinal periodicity of the interferogram, the setup is insensitive to mechanical drift and vibrations. The effect of temporal coherence of x-ray source is investigated and its related bandwidth is derived. Based on the theory of partially coherent light, it shows that the requirement for the spatial coherence of x-ray source is not strict and can be met by the general microfocus x-ray tube for x-ray differential phase-contrast imaging.展开更多
In-line phase-contrast computed tomography（IL-PC-CT） imaging is a new physical and biochemical imaging method.IL-PC-CT has advantages compared to absorption CT when imaging soft tissues. In practical applications, r...In-line phase-contrast computed tomography（IL-PC-CT） imaging is a new physical and biochemical imaging method.IL-PC-CT has advantages compared to absorption CT when imaging soft tissues. In practical applications, ring artifacts which will reduce the image quality are commonly encountered in IL-PC-CT, and numerous correction methods exist to either pre-process the sinogram or post-process the reconstructed image. In this study, we develop an IL-PC-CT reconstruction method based on anisotropic total variation（TV） minimization. Using this method, the ring artifacts are corrected during the reconstruction process. This method is compared with two methods： a sinogram preprocessing correction technique based on wavelet-FFT filter and a reconstruction method based on isotropic TV. The correction results show that the proposed method can reduce visible ring artifacts while preserving the liver section details for real liver section synchrotron data.展开更多
A total of 50 healthy volunteers aged between 18 and 54 years underwent phase-contrast cine MR to assess cerebrospinal fluid flow characteristics in different regions of the vertebral canal. The results revealed that ...A total of 50 healthy volunteers aged between 18 and 54 years underwent phase-contrast cine MR to assess cerebrospinal fluid flow characteristics in different regions of the vertebral canal. The results revealed that the cerebrospinal fluid peak flow velocity and peak flow rate in the systolic phase were significantly greater than those in the diastolic phase at the same level in the subarachnoid space of the cervical spinal canal. The ventral peak flow velocity and peak flow rate were significantly greater than the post-lateral peak flow velocity and flow rate, while there were no differences between left and right post-lateral subarachnoid peak velocity and flow rate. Moreover, there were no significant differences in peak flow velocity and peak flow rate between the systolic and diastolic phases, ventral, right post-lateral or left post-lateral peak flow velocity and peak flow rate at the same level in the subarachnoid space of the cervical spinal canal among different age groups （18 24, 25 34, 35-44, ≥45 years）.展开更多
This study investigated the effect of velocity encoding on measurement of brain blood flow and blood volume of inflow and outflow using phase-contrast magnetic resonance angiography. A single two-dimensional phase-con...This study investigated the effect of velocity encoding on measurement of brain blood flow and blood volume of inflow and outflow using phase-contrast magnetic resonance angiography. A single two-dimensional phase-contrast magnetic resonance angiography slice was applied perpendicular to the internal carotid artery and the vertebral artery at C2 level. For each subject, the velocity encoding was set from 30 to 90 cm/s with an interval of 10 cm/s for a total of seven settings. Various velocity encodings greatly affected blood flow volume, maximal blood flow velocity and mean blood flow velocity in the internal carotid artery, but did not significantly affect vertebral arteries and jugular veins. When velocity encoding was 60-80 cm/s, the inflow blood volume was 655 _＋ 118 mL/min, and the outflow volume was 506 _＋ 186 mL/min. The ratio of outflow/inflow was steady at 0.78-0.83, and there was no aliasing in any of the images. These findings suggest that velocity encodings of 60 80 cm/s should be selected during measurement of cerebral blood flow volume using phase-contrast magnetic resonance angiography.展开更多
Grating-based x-ray phase contrast imaging has attracted increasing interest in recent decades as multimodal and laboratory source usable method.Specific efforts have been focused on establishing a new extraction meth...Grating-based x-ray phase contrast imaging has attracted increasing interest in recent decades as multimodal and laboratory source usable method.Specific efforts have been focused on establishing a new extraction method to perform practical applications.In this work,noise properties of multi-combination information of newly established information extraction method,so-called angular signal radiography method,are investigated to provide guidelines for targeted and specific applications.The results show that how multi-combination of images can be used in targeted practical applications to obtain a high-quality image in terms of signal-to-noise ratio.Our conclusions can also hold true for upcoming targeted practical applications such as biomedical imaging,non-destructive imaging,and materials science.展开更多
In the past decade, phase-contrast imaging (PCI) has become a hot research with an increased improvement of the image contrast with respect to conventional absorption radiography. In this paper, effects of tube voltag...In the past decade, phase-contrast imaging (PCI) has become a hot research with an increased improvement of the image contrast with respect to conventional absorption radiography. In this paper, effects of tube voltage (kVp) on propagation-based phase-contrast imaging have been investigated with two types of microfocus x-ray tubes, a conventional sealed x-ray tube with the focal spot size of 13 - 20 μm and an open x-ray tube with minimum focal spot size less than 2 μm. A cooled x-ray CCD detector with the pixel size of 24 μm was used to acquire digital images. Two thin plastic sheets with different thickness were used as radiography phantoms. Two different phenomena were observed for the two x-ray tubes. For the open tube, phase-contrast effect has a slight drop with the increasing of tube voltage, however, it is opposite for the sealed tube. A further investigation indicates that the variation of focal spot size causes the abnormal result for the sealed tube. It also shows that phase-contrast effect is more sensitive to focal spot size than tube voltage.展开更多
Objective To evaluate the application of phasecontrast cine magnetic resonance imaging (MRI) in endoscopic aqueductoplasty for patients with obstructive hydrocephalus. Methods The clinical diagnosis of hydrocephalus d...Objective To evaluate the application of phasecontrast cine magnetic resonance imaging (MRI) in endoscopic aqueductoplasty for patients with obstructive hydrocephalus. Methods The clinical diagnosis of hydrocephalus due to aqueduct obstruction in 23 patients was confirmed by phase contrast cine MRI examination.展开更多
Imaging blood vessels is of importance for determining the vascular distribution of organs and tumors.Phase-contrast X-ray imaging can reveal the vessels in much more detail than conventional X-ray absorption method.V...Imaging blood vessels is of importance for determining the vascular distribution of organs and tumors.Phase-contrast X-ray imaging can reveal the vessels in much more detail than conventional X-ray absorption method.Visualizing murine liver microvasculature ex vivo with phase-contrast X-ray imaging was performed at Shanghai Synchrotron Radiation Facility.Barium sulfate and physiological saline were used as contrast agents for the blood vessels.Blood vessels of <Φ20μm could be detected by replacing resident blood with physiological saline or barium sulfate.An entire branch of the portal vein (from the main axial portal vein to the ninth generation of branching) could be captured in a single phase-contrast image.It is demonstrated that selective angiography based on phase contrast X-ray imaging,with a physiological material of low Z elements (such as saline) being the contrast agent,is a viable imaging strategy.Further efforts will be focused on using the technique to image tumor angiogenesis.展开更多
An x-ray scintillator screen with a special structure, functioning as detector and analyser grating, was proposed for collecting the interferogram of differential phase contrast imaging without absorption grating and ...An x-ray scintillator screen with a special structure, functioning as detector and analyser grating, was proposed for collecting the interferogram of differential phase contrast imaging without absorption grating and difficulty of fabrication by a state of the art technique. On the basis of phase grating diffraction, a detecting model of the scintillator screen was built for analysing the phase and absorption information of objects. According to the analysis, a new method of phase retrievals based on two-images and the optimal structure of screen were presented.展开更多
Rationale and Objectives: Computational fluid dynamic (CFD) simulations are discussed with respect to their potential for quality assurance of flow quantification using commercial software for the evaluation of magnet...Rationale and Objectives: Computational fluid dynamic (CFD) simulations are discussed with respect to their potential for quality assurance of flow quantification using commercial software for the evaluation of magnetic resonance phase contrast angiography (PCA) data. Materials and Methods: Magnetic resonance phase contrast angiography data was evaluated with the Nova software. CFD simulations were performed on that part of the vessel system where the flow behavior was unexpected or non-reliable. The CFD simulations were performed with in-house written software. Results: The numerical CFD calculations demonstrated that under reasonable boundary conditions, defined by the PCA velocity values, the flow behavior within the critical parts of the vessel system can be correctly reproduced. Conclusion: CFD simulations are an important extension to commercial flow quantification tools with regard to quality assurance.展开更多
Objectives To reveal the relationship of brain motion and cerebrospinal fluid (CSF) flow by phase-contrast cine MRI, and to evaluate this technique in differentiating between arachnoid cysts and subarachnoid space en...Objectives To reveal the relationship of brain motion and cerebrospinal fluid (CSF) flow by phase-contrast cine MRI, and to evaluate this technique in differentiating between arachnoid cysts and subarachnoid space enlargement. Methods Using a phase-contrast cine MRI pulse sequence, we measured brain motion and CSF flow during the cardiac cycle in 10 healthy volunteers and 10 patients with MRI-suspected arachnoid cyst or subarachnoid space enlargement. CSF stroke volume curve was illustrated according to flow quantification, and time-signal intensity curve was traced. The two curves were compared. Results This study showed that brain motion was due to the volume difference between arterial and venous blood flow during a cardiac cycle, and thus drives CSF pulsation. Arachnoid cysts and subarachnoid space enlargement carried different curve patterns, demonstrating that phase-contrast MRI and flow quantification can be a useful and reliable technique for non-invasive evaluation of brain motion and CSF flow. Conclusion Arachnoid cysts can be successfully differentiated using phase-contrast cine MRI from subarachnoid space enlargement.展开更多
Several X-ray phase visualization methods are being real- ized for imaging of phase objects, such as biological and polymeric specimens. Grating-based phase-contrast imaging using a source-grating-attached X-ray tube ...Several X-ray phase visualization methods are being real- ized for imaging of phase objects, such as biological and polymeric specimens. Grating-based phase-contrast imaging using a source-grating-attached X-ray tube that provides partially coherent X rays is one of the most successful methods in this field.展开更多
Propagation-based phase-contrast imaging was simulated based on paraxial Fresnel-Kirchoff diffraction integral and spherical wave illumination. Under a developed micro-CT system parameters, the effects of focal-spot s...Propagation-based phase-contrast imaging was simulated based on paraxial Fresnel-Kirchoff diffraction integral and spherical wave illumination. Under a developed micro-CT system parameters, the effects of focal-spot size and imaging geometry on phase-contrast imaging have been investigated using a 2-mm-thickness polystyrene edge phantom. An equivalent mono-energy was used to substitute the polychromatic spectrum of the micro-focus X-ray source. To consider effects of focal-spot size and detector resolution, the obtained phase-contrast image with an ideal point source was convolved with source intensity distribution and point spread function of detector. Simulations show reasonable influences of the two parameters which are in good agreement with experimental results.展开更多
Red blood cell(RBC)indices serve as clinically important parameters for diagnosing various blood-related diseases.Conventional hematology analyzers provide the highly accurate detection of RBC indices but require larg...Red blood cell(RBC)indices serve as clinically important parameters for diagnosing various blood-related diseases.Conventional hematology analyzers provide the highly accurate detection of RBC indices but require large blood volumes(>1 mL),and the results are bulk mean values averaged over a large number of RBCs.Moreover,they do not provide quantitative information related to the morphological and chemical alteration of RBCs at the single-cell level.Recently,quantitative phase imaging(QPI)methods have been introduced as viable detection platforms for RBC indices.However,coherent QPI methods are built on complex optical setups and suffer from coherent speckle noise,which limits their detection accuracy and precision.Here,we present spectroscopic differential phase-contrast(sDPC)microscopy as a platform for measuring RBC indices.sDPC is a computational microscope that produces color-dependent phase images with higher spatial resolution and reduced speckle noise compared to coherent QPIs.Using these spectroscopic phase images and computational algorithms,RBC indices can be extracted with high accuracy.We experimentally demonstrate that sDPC enables the high-accuracy measurement of the mean corpuscular hemoglobin concentration,mean corpuscular volume,mean corpuscular hemoglobin,red cell distribution width,hematocrit,hemoglobin concentration,and RBC count with errors smaller than 7%as compared to a clinical hematology analyzer based on flow cytometry(XN-2000;Sysmex,Kobe,Japan).We further validate the clinical utility of the sDPC method by measuring and comparing the RBC indices of the control and anemic groups against those obtained using the clinical hematology analyzer.展开更多
Employing the approximation theory based on refraction and the definition of the total pointspread-function of the imaging system, the variation in the edge contrast of simple model samples is discussed with different...Employing the approximation theory based on refraction and the definition of the total pointspread-function of the imaging system, the variation in the edge contrast of simple model samples is discussed with different source-to-sample and sample-to-detector distances, which actually means different spatial resolutions of the imaging system. The experiments were carried out with the Beamline 4W1A imaging setup at the Beijing Synchrotron Radiation Facility for simple model and insect samples. The results show that to obtain clear phase-contrast images of biologic tissues for the X-ray in-line imaging setup, with determined parameters such as the size of the X-ray source, the pixel size of the detector and the fixed source-to-sample distance, there is a range of optimized sample-to-detector distances. The analysis method discussed in this article can be helpful in optimizing the setup of X-ray in-line phase-contrast imaging.展开更多
基金supported by the National Natural Science Foundation of China(Nos.U2032148,U2032157,11775224)USTC Research Funds of the Double First-Class Initiative(No.YD2310002008)the National Key Research and Development Program of China(No.2017YFA0402904),the Youth Innovation Promotion Association,CAS(No.2020457)。
文摘Grating-based X-ray phase-contrast imaging enhances the contrast of imaged objects,particularly soft tissues.However,the radiation dose in computed tomography(CT)is generally excessive owing to the complex collection scheme.Sparse-view CT collection reduces the radiation dose,but with reduced resolution and reconstructed artifacts particularly in analytical reconstruction methods.Recently,deep learning has been employed in sparse-view CT reconstruction and achieved stateof-the-art results.Nevertheless,its low generalization performance and requirement for abundant training datasets have hindered the practical application of deep learning in phase-contrast CT.In this study,a CT model was used to generate a substantial number of simulated training datasets,thereby circumventing the need for experimental datasets.By training a network with simulated training datasets,the proposed method achieves high generalization performance in attenuationbased CT and phase-contrast CT,despite the lack of sufficient experimental datasets.In experiments utilizing only half of the CT data,our proposed method obtained an image quality comparable to that of the filtered back-projection algorithm with full-view projection.The proposed method simultaneously addresses two challenges in phase-contrast three-dimensional imaging,namely the lack of experimental datasets and the high exposure dose,through model-driven deep learning.This method significantly accelerates the practical application of phase-contrast CT.
基金the Medical Program of Scientific & Technical Foundation in Xiamen in 2008, No. 3502Z20084028
文摘The optimal velocity encoding of phase-contrast magnetic resonance angiography （PC MRA） in measuring cerebral blood flow volume （BFV） ranges from 60 to 80 cm/s. To verify the accuracy of two-dimensional （2D） PC MRA, the present study localized the region of interest at blood vessels of the neck using PC MRA based on three-dimensional time-of-flight sequences, and the velocity encoding was set to 80 cm/s. Results of the measurements showed that the error rate was 7.0±6.0% in the estimation of BFV in the internal carotid artery, the external carotid artery and the ipsilateral common carotid artery. There was no significant difference, and a significant correlation in BFV between internal carotid artery ＋ external carotid artery and ipsilateral common carotid artery. In addition, the BFV of the common carotid artery was correlated with that of the ipsilateral internal carotid artery. The main error was attributed to the external carotid artery and its branches. Therefore, after selecting the appropriate scanning parameters and protocols, 2D PC MRA is more accurate in the determination of BFV in the carotid arteries.
文摘This paper gives theoretical analysis of visibility of fringes, which is influenced by distances, temporal and spatial coherence of source, in hard x-ray differential phase-contrast imaging with microfocus x-ray source. According to the character of longitudinal periodicity of the interferogram, the setup is insensitive to mechanical drift and vibrations. The effect of temporal coherence of x-ray source is investigated and its related bandwidth is derived. Based on the theory of partially coherent light, it shows that the requirement for the spatial coherence of x-ray source is not strict and can be met by the general microfocus x-ray tube for x-ray differential phase-contrast imaging.
基金supported by the National Natural Science Foundation of China(Grant Nos.61671004,61271012,81371549,81671683,and 11501415)the Natural Science Foundation of Tianjin City,China(Grant No.16JCYBJC28600)+4 种基金the WBE Liver Fibrosis Foundation of China(Grant No.CFHPC20131033)the Instrument Developing Project of the Chinese Academy of Sciences(Grant No.YZ201410)the Foundation of Tianjin University of Technology and Education(Grant Nos.KJ11-22 and J10011060321)SRF for ROCS,SEMthe IHEP-CAS Scientific Research Foundation(Grant No.2013IHEPYJRC801)
文摘In-line phase-contrast computed tomography（IL-PC-CT） imaging is a new physical and biochemical imaging method.IL-PC-CT has advantages compared to absorption CT when imaging soft tissues. In practical applications, ring artifacts which will reduce the image quality are commonly encountered in IL-PC-CT, and numerous correction methods exist to either pre-process the sinogram or post-process the reconstructed image. In this study, we develop an IL-PC-CT reconstruction method based on anisotropic total variation（TV） minimization. Using this method, the ring artifacts are corrected during the reconstruction process. This method is compared with two methods： a sinogram preprocessing correction technique based on wavelet-FFT filter and a reconstruction method based on isotropic TV. The correction results show that the proposed method can reduce visible ring artifacts while preserving the liver section details for real liver section synchrotron data.
基金supported by the Science and Technology Support Program of Hebei Province(Cervical spinal canal cerebrospinal fluid movement using 3.0TMR fast phase-contrast cine imaging),No.112061179D
文摘A total of 50 healthy volunteers aged between 18 and 54 years underwent phase-contrast cine MR to assess cerebrospinal fluid flow characteristics in different regions of the vertebral canal. The results revealed that the cerebrospinal fluid peak flow velocity and peak flow rate in the systolic phase were significantly greater than those in the diastolic phase at the same level in the subarachnoid space of the cervical spinal canal. The ventral peak flow velocity and peak flow rate were significantly greater than the post-lateral peak flow velocity and flow rate, while there were no differences between left and right post-lateral subarachnoid peak velocity and flow rate. Moreover, there were no significant differences in peak flow velocity and peak flow rate between the systolic and diastolic phases, ventral, right post-lateral or left post-lateral peak flow velocity and peak flow rate at the same level in the subarachnoid space of the cervical spinal canal among different age groups （18 24, 25 34, 35-44, ≥45 years）.
基金the Medical Program of the Scientific & Technical Foundation in Xiamen(MRI study of chronic cerebrovascular insufficiency) in 2008,No.3502Z20084028
文摘This study investigated the effect of velocity encoding on measurement of brain blood flow and blood volume of inflow and outflow using phase-contrast magnetic resonance angiography. A single two-dimensional phase-contrast magnetic resonance angiography slice was applied perpendicular to the internal carotid artery and the vertebral artery at C2 level. For each subject, the velocity encoding was set from 30 to 90 cm/s with an interval of 10 cm/s for a total of seven settings. Various velocity encodings greatly affected blood flow volume, maximal blood flow velocity and mean blood flow velocity in the internal carotid artery, but did not significantly affect vertebral arteries and jugular veins. When velocity encoding was 60-80 cm/s, the inflow blood volume was 655 _＋ 118 mL/min, and the outflow volume was 506 _＋ 186 mL/min. The ratio of outflow/inflow was steady at 0.78-0.83, and there was no aliasing in any of the images. These findings suggest that velocity encodings of 60 80 cm/s should be selected during measurement of cerebral blood flow volume using phase-contrast magnetic resonance angiography.
基金Project supported by the National Natural Science Foundation of China(Grant No.11535015)the National Special Foundation of China for Major Science Instrument(Grant No.61227802)+3 种基金the National Natural Science Foundation of China(Grant Nos.61405120,61605119,61571305,and 11674232)the Natural Science Foundation of Shenzhen,China(Grant No.JCYJ20170302142617703)the Natural Science Foundation of Shenzhen University,China(Grant Nos.2017017 and 2018041)sponsored by the Post-doctoral International Exchange Program of China
文摘Grating-based x-ray phase contrast imaging has attracted increasing interest in recent decades as multimodal and laboratory source usable method.Specific efforts have been focused on establishing a new extraction method to perform practical applications.In this work,noise properties of multi-combination information of newly established information extraction method,so-called angular signal radiography method,are investigated to provide guidelines for targeted and specific applications.The results show that how multi-combination of images can be used in targeted practical applications to obtain a high-quality image in terms of signal-to-noise ratio.Our conclusions can also hold true for upcoming targeted practical applications such as biomedical imaging,non-destructive imaging,and materials science.
文摘In the past decade, phase-contrast imaging (PCI) has become a hot research with an increased improvement of the image contrast with respect to conventional absorption radiography. In this paper, effects of tube voltage (kVp) on propagation-based phase-contrast imaging have been investigated with two types of microfocus x-ray tubes, a conventional sealed x-ray tube with the focal spot size of 13 - 20 μm and an open x-ray tube with minimum focal spot size less than 2 μm. A cooled x-ray CCD detector with the pixel size of 24 μm was used to acquire digital images. Two thin plastic sheets with different thickness were used as radiography phantoms. Two different phenomena were observed for the two x-ray tubes. For the open tube, phase-contrast effect has a slight drop with the increasing of tube voltage, however, it is opposite for the sealed tube. A further investigation indicates that the variation of focal spot size causes the abnormal result for the sealed tube. It also shows that phase-contrast effect is more sensitive to focal spot size than tube voltage.
文摘Objective To evaluate the application of phasecontrast cine magnetic resonance imaging (MRI) in endoscopic aqueductoplasty for patients with obstructive hydrocephalus. Methods The clinical diagnosis of hydrocephalus due to aqueduct obstruction in 23 patients was confirmed by phase contrast cine MRI examination.
基金Supported by National Basic Research Program of China (973 Program Grant No.2010CB834305)
文摘Imaging blood vessels is of importance for determining the vascular distribution of organs and tumors.Phase-contrast X-ray imaging can reveal the vessels in much more detail than conventional X-ray absorption method.Visualizing murine liver microvasculature ex vivo with phase-contrast X-ray imaging was performed at Shanghai Synchrotron Radiation Facility.Barium sulfate and physiological saline were used as contrast agents for the blood vessels.Blood vessels of <Φ20μm could be detected by replacing resident blood with physiological saline or barium sulfate.An entire branch of the portal vein (from the main axial portal vein to the ninth generation of branching) could be captured in a single phase-contrast image.It is demonstrated that selective angiography based on phase contrast X-ray imaging,with a physiological material of low Z elements (such as saline) being the contrast agent,is a viable imaging strategy.Further efforts will be focused on using the technique to image tumor angiogenesis.
基金supported by the National Natural Science Foundation of China (Grant Nos. 60232090 and 10774102)the Science & Technology Project from Shenzhen Government of China (Grant Nos. 2008340 and 200717)
文摘An x-ray scintillator screen with a special structure, functioning as detector and analyser grating, was proposed for collecting the interferogram of differential phase contrast imaging without absorption grating and difficulty of fabrication by a state of the art technique. On the basis of phase grating diffraction, a detecting model of the scintillator screen was built for analysing the phase and absorption information of objects. According to the analysis, a new method of phase retrievals based on two-images and the optimal structure of screen were presented.
文摘Rationale and Objectives: Computational fluid dynamic (CFD) simulations are discussed with respect to their potential for quality assurance of flow quantification using commercial software for the evaluation of magnetic resonance phase contrast angiography (PCA) data. Materials and Methods: Magnetic resonance phase contrast angiography data was evaluated with the Nova software. CFD simulations were performed on that part of the vessel system where the flow behavior was unexpected or non-reliable. The CFD simulations were performed with in-house written software. Results: The numerical CFD calculations demonstrated that under reasonable boundary conditions, defined by the PCA velocity values, the flow behavior within the critical parts of the vessel system can be correctly reproduced. Conclusion: CFD simulations are an important extension to commercial flow quantification tools with regard to quality assurance.
文摘Objectives To reveal the relationship of brain motion and cerebrospinal fluid (CSF) flow by phase-contrast cine MRI, and to evaluate this technique in differentiating between arachnoid cysts and subarachnoid space enlargement. Methods Using a phase-contrast cine MRI pulse sequence, we measured brain motion and CSF flow during the cardiac cycle in 10 healthy volunteers and 10 patients with MRI-suspected arachnoid cyst or subarachnoid space enlargement. CSF stroke volume curve was illustrated according to flow quantification, and time-signal intensity curve was traced. The two curves were compared. Results This study showed that brain motion was due to the volume difference between arterial and venous blood flow during a cardiac cycle, and thus drives CSF pulsation. Arachnoid cysts and subarachnoid space enlargement carried different curve patterns, demonstrating that phase-contrast MRI and flow quantification can be a useful and reliable technique for non-invasive evaluation of brain motion and CSF flow. Conclusion Arachnoid cysts can be successfully differentiated using phase-contrast cine MRI from subarachnoid space enlargement.
基金supported by the research fund of Dankook University(No.R000122495)
文摘Several X-ray phase visualization methods are being real- ized for imaging of phase objects, such as biological and polymeric specimens. Grating-based phase-contrast imaging using a source-grating-attached X-ray tube that provides partially coherent X rays is one of the most successful methods in this field.
基金National Natural Science Foundation of Chinagrant number:61002041,61102161+3 种基金National 973 Basic Research Program of Chinagrant number:2010CB7326002010-Guangdong Province Innovational Research Team Program and Programs of Shenzhengrant number:JC201005270313A,JC201005280493A,JC201005280581A,ZYA201006300034A
文摘Propagation-based phase-contrast imaging was simulated based on paraxial Fresnel-Kirchoff diffraction integral and spherical wave illumination. Under a developed micro-CT system parameters, the effects of focal-spot size and imaging geometry on phase-contrast imaging have been investigated using a 2-mm-thickness polystyrene edge phantom. An equivalent mono-energy was used to substitute the polychromatic spectrum of the micro-focus X-ray source. To consider effects of focal-spot size and detector resolution, the obtained phase-contrast image with an ideal point source was convolved with source intensity distribution and point spread function of detector. Simulations show reasonable influences of the two parameters which are in good agreement with experimental results.
基金supported by the Samsung Research Funding&Incubation Center of Samsung Electronics under Project SRFC-IT2002-07the National Research Foundation of Korea(NRF)Grant funded by the government of Korea(MSIT)(2023R1A2C3004040)+3 种基金the Korea Institute for Advancement of Technology(KIAT)Grant funded by the government of Korea(MOTIE)(P0019784)the Ministry of Science and ICT(Project 2023-22030004-20)(NTIS,1711179106)the Commercialization Promotion Agency for R&D Outcomes(COMPA)funded by the Ministry of Science and ICT(MSIT)(1711198541,development of key optical technologies of inspection and measurement for the analysis of 3D complex nanostructures)the Korea Medical Device Development Fund(KMDF_PR_20200901_0099,Project Number:9991007255).
文摘Red blood cell(RBC)indices serve as clinically important parameters for diagnosing various blood-related diseases.Conventional hematology analyzers provide the highly accurate detection of RBC indices but require large blood volumes(>1 mL),and the results are bulk mean values averaged over a large number of RBCs.Moreover,they do not provide quantitative information related to the morphological and chemical alteration of RBCs at the single-cell level.Recently,quantitative phase imaging(QPI)methods have been introduced as viable detection platforms for RBC indices.However,coherent QPI methods are built on complex optical setups and suffer from coherent speckle noise,which limits their detection accuracy and precision.Here,we present spectroscopic differential phase-contrast(sDPC)microscopy as a platform for measuring RBC indices.sDPC is a computational microscope that produces color-dependent phase images with higher spatial resolution and reduced speckle noise compared to coherent QPIs.Using these spectroscopic phase images and computational algorithms,RBC indices can be extracted with high accuracy.We experimentally demonstrate that sDPC enables the high-accuracy measurement of the mean corpuscular hemoglobin concentration,mean corpuscular volume,mean corpuscular hemoglobin,red cell distribution width,hematocrit,hemoglobin concentration,and RBC count with errors smaller than 7%as compared to a clinical hematology analyzer based on flow cytometry(XN-2000;Sysmex,Kobe,Japan).We further validate the clinical utility of the sDPC method by measuring and comparing the RBC indices of the control and anemic groups against those obtained using the clinical hematology analyzer.
基金Supported by the National Natural Science Foundation of China (10475090)
文摘Employing the approximation theory based on refraction and the definition of the total pointspread-function of the imaging system, the variation in the edge contrast of simple model samples is discussed with different source-to-sample and sample-to-detector distances, which actually means different spatial resolutions of the imaging system. The experiments were carried out with the Beamline 4W1A imaging setup at the Beijing Synchrotron Radiation Facility for simple model and insect samples. The results show that to obtain clear phase-contrast images of biologic tissues for the X-ray in-line imaging setup, with determined parameters such as the size of the X-ray source, the pixel size of the detector and the fixed source-to-sample distance, there is a range of optimized sample-to-detector distances. The analysis method discussed in this article can be helpful in optimizing the setup of X-ray in-line phase-contrast imaging.
