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油菜种子特异表达启动子pNapB和pFAE1的结构与功能比较研究 被引量:7
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作者 肖娜 武玉花 +2 位作者 肖玲 吴刚 卢长明 《中国油料作物学报》 CAS CSCD 北大核心 2008年第1期1-9,共9页
启动子是基因在转录水平上的一种重要调控元件。本研究通过同源序列法从甘蓝型油菜品种中油821中分离到油菜基因NapB和FAE1的上游启动子序列pNapB和pFAE1,其中pNapB长1136bp,pFAE1长1420bp。两种启动子的核苷酸序列都含有种子特异表达... 启动子是基因在转录水平上的一种重要调控元件。本研究通过同源序列法从甘蓝型油菜品种中油821中分离到油菜基因NapB和FAE1的上游启动子序列pNapB和pFAE1,其中pNapB长1136bp,pFAE1长1420bp。两种启动子的核苷酸序列都含有种子特异表达启动子的顺式作用元件,包括RY重复、G-box和E-box等,但两种启动子顺式作用元件的序列分布不同。将pNapB和pFAE1两个启动子分别与报告基因GUS融合并通过农杆菌介导法导入烟草,得到大量转基因烟草植株。对T1代转基因烟草进行组织化学分析,比较了不同启动子的组织表达特异性。结果表明,两种启动子都只在种子中起作用,属于典型的种子特异性表达启动子,但是它们作用的时间和空间分布以及作用强度明显不同。pNapB启动子比pFAE1作用开始的时间早,持续时间长,在早期作用比pFAE1强,但pFAE1启动子在种子发育中期启动速度快,成熟种子的GUS染色深度在两种启动子间差别不大。 展开更多
关键词 种子特异表达启动子 pNapB pfae1 遗传转化 GUS 转基因烟草
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Properties of Creeping Streamer Progressed in Dielectric Barrier with Narrow Gap in PFAE Oil
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作者 Fri Murdiya Ryoichi Hanaoka +2 位作者 Ryota Hashi Katsunori Miyagi Hidenobu Koide 《Journal of Energy and Power Engineering》 2013年第7期1257-1264,共8页
The behaviors of creeping streamer progressed in the narrow gap with the range of 0.1 mm to 0.2 mm between the pressboard mounted a BSE (back side electrode) and the acrylic resin plate were carried out in PFAE (pa... The behaviors of creeping streamer progressed in the narrow gap with the range of 0.1 mm to 0.2 mm between the pressboard mounted a BSE (back side electrode) and the acrylic resin plate were carried out in PFAE (palm fatty acid ester) oil under variable impulse voltage up to -4-140 kVpeak with +1.2/50 μs and ±1.2/1,000μs waveforms. The growth of positive and negative streamers depends on the gap space between two solid dielectrics. And the distinctive polarities of impulse voltage affect in the streamer length and flashover voltage between needle and counter electrodes as well. By decreasing the gap space, the flashover voltage largely increased while the mean velocity of positive streamer significantly decreased under the both polarities of impulse voltage. These results have been compared to commercial mineral oil (transformer oil: JIS-C2320). It is shown that the behaviors of creeping discharge in both oils elegantly reveal the distinctive phenomena. 展开更多
关键词 pfae oil mineral oil impulse creeping streamer narrow gap flashover voltage.
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Impulse Creeping Streamer and Propagation Velocity in Vegetable and Mineral Oils with Pressboard Interface
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作者 Ryoichi Hanaoka Katsunori Miyagi +3 位作者 Yoji Fujita Kiyoshi Takamoto Seiichi Nishikawa Yoshiyuki Oura 《Journal of Energy and Power Engineering》 2018年第7期346-355,共10页
This paper presents an experimental study on the creeping discharge propagating over the pressboard surface in two vegetable oils(PFAE(palm fatty acid ester)oil and CRS(crude rapeseed)oil)and commercial mineral oil un... This paper presents an experimental study on the creeping discharge propagating over the pressboard surface in two vegetable oils(PFAE(palm fatty acid ester)oil and CRS(crude rapeseed)oil)and commercial mineral oil under the quasi-square impulse voltage with any pulse width.The pressboard impregnated with the sample oil is immersed completely into the same oil.The tungsten needle electrode is installed in the pressboard surface with and without the counter electrode to generate a creeping discharge.The other side of pressboard has the thin copper rod as a back side electrode.A comparison of the shape and stopping length of positive and negative streamers,discharge current,emitted light signal,and temporal variation and velocity of streamer propagation is reported for all different oil-pressboard interfaces.It has been shown that the behavior of creeping streamers has unique characteristics and polarity effects,and the traveling mode and propagation velocity of streamers are greatly different depending on the type of oil. 展开更多
关键词 CREEPING STREAMER STOPPING length propagation velocity quasi-square IMPULSE voltage pfae OIL CRS OIL mineral OIL
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