BACKGROUND This study analyzed the dental follicle and alveolar bone of two patients with tooth eruption disorders,aiming to provide some reference for exploring the etiology and selecting treatment plans of this dise...BACKGROUND This study analyzed the dental follicle and alveolar bone of two patients with tooth eruption disorders,aiming to provide some reference for exploring the etiology and selecting treatment plans of this disease from the perspective of the influence of extracellular matrix on osteoclasts differentiation in dental follicle.CASE SUMMARY Collect dental follicle and alveolar bone tissue from one patient with single tooth eruption disorder and one patient with full permanent tooth eruption disorder,respectively.Simultaneously collect the dental follicle and alveolar bone tissue of obstructed teeth that need to be extracted due to orthodontic treatment as the control group.Hematoxylin and eosin(HE)staining was used to observe the morphology of dental follicle cells.Immunohistochemical staining was used to observe the expression of periostin,receptor activator of nuclear factor kappa B ligand(RANKL),and osteoprotegerin(OPG)protein in dental follicle and alveolar bone tissue.And observe the eruption of teeth after removing resistance from the crown of the permanent tooth germ.CONCLUSION HE staining of two cases of dental follicle tissues showed that the volume of dental follicle cells decreased,the nuclei were condensed,and there seemed to be cellular fibrosis.The immunohistochemical staining showed that both the dental follicle and alveolar bone tissue exhibited increased expression of periostin,decreased expression of RANKL and OPG proteins,and decreased RANKL/OPG ratio.After removing resistance,the permanent tooth germ often appears to have normal eruption.Tooth eruption disorders may be accompanied by abnormal remodeling of periostin,which affects the differentiation function of osteoclasts in the dental follicle and leads to metabolic imbalance of alveolar bone,resulting in tooth eruption disorders.Whether it is a single or full permanent tooth eruption disorder,once the coronal resistance is removed,the teeth can often erupt normally.展开更多
目的探讨24 h动态心电图联合骨膜蛋白(Periostin)、乳糖凝集素-3(Gal-3)对急性心肌梗死(AMI)后恶性心律失常(MA)的预测价值。方法选择2020年1月-2022年12月皖南医学院第一附属医院心血管内科收治的AMI患者367例为研究对象(AMI组),根据...目的探讨24 h动态心电图联合骨膜蛋白(Periostin)、乳糖凝集素-3(Gal-3)对急性心肌梗死(AMI)后恶性心律失常(MA)的预测价值。方法选择2020年1月-2022年12月皖南医学院第一附属医院心血管内科收治的AMI患者367例为研究对象(AMI组),根据是否发生MA分为非MA亚组(n=286)和MA亚组(n=81)。另选取医院同期健康体检志愿者370例为健康对照组。酶联免疫吸附试验(ELISA)检测血清Periostin、Gal-3水平;多因素Logistic回归分析AMI患者发生MA的影响因素;受试者工作特征(ROC)曲线分析Periostin、Gal-3、24 h QT间期变异性(24 h QTV)、24 h正常心房开始除极至心室开始除极时间间期标准差(SDNN)对AMI患者发生MA的预测价值。结果与健康对照组比较,AMI组血清Periostin、Gal-3水平均升高(t/P=59.225/<0.001、33.141/<0.001);与非MA亚组比较,MA亚组血清Periostin、Gal-3水平及低血钾比例均升高(t/χ^(2)/P=7.602/<0.001、6.049/<0.001、15.167/<0.001);与非MA亚组比较,MA亚组SDNN、24 h QTV、24 h连续5 min正常R-R间期标准差(SDANN-index)、24 h连续5 min正常R-R间期标准差均值(SDNN-index)水平降低(t/P=7.029/<0.001、7.401/<0.001、4.281/<0.001、4.721/<0.001);血清Periostin、Gal-3水平升高是AMI患者发生MA的危险因素[OR(95%CI)=2.163(1.416~3.303)、2.450(1.570~3.823)],24 h QTV、SDNN水平升高为保护因素[OR(95%CI)=0.449(0.264~0.764)、0.430(0.223~0.829)];24 h QTV、SDNN、Periostin、Gal-3单独及四项联合预测AMI患者发生MA的AUC分别为0.791、0.719、0.758、0.744、0.901,四者联合预测的AUC高于单独预测(Z/P=3.916/<0.001、6.101/<0.001、4.888/<0.001、5.764/<0.001)。结论AMI患者血清Periostin、Gal-3水平均升高,24 h动态心电图指标联合血清Periostin、Gal-3预测AMI患者发生MA的效能较高。展开更多
基金Supported by The Xuzhou Medical University Affiliated Hospital Development Fund Support Project,No.XYFM202457the 521 Scientific Research Project of Lianyungang City,No.LYG 06521202362.
文摘BACKGROUND This study analyzed the dental follicle and alveolar bone of two patients with tooth eruption disorders,aiming to provide some reference for exploring the etiology and selecting treatment plans of this disease from the perspective of the influence of extracellular matrix on osteoclasts differentiation in dental follicle.CASE SUMMARY Collect dental follicle and alveolar bone tissue from one patient with single tooth eruption disorder and one patient with full permanent tooth eruption disorder,respectively.Simultaneously collect the dental follicle and alveolar bone tissue of obstructed teeth that need to be extracted due to orthodontic treatment as the control group.Hematoxylin and eosin(HE)staining was used to observe the morphology of dental follicle cells.Immunohistochemical staining was used to observe the expression of periostin,receptor activator of nuclear factor kappa B ligand(RANKL),and osteoprotegerin(OPG)protein in dental follicle and alveolar bone tissue.And observe the eruption of teeth after removing resistance from the crown of the permanent tooth germ.CONCLUSION HE staining of two cases of dental follicle tissues showed that the volume of dental follicle cells decreased,the nuclei were condensed,and there seemed to be cellular fibrosis.The immunohistochemical staining showed that both the dental follicle and alveolar bone tissue exhibited increased expression of periostin,decreased expression of RANKL and OPG proteins,and decreased RANKL/OPG ratio.After removing resistance,the permanent tooth germ often appears to have normal eruption.Tooth eruption disorders may be accompanied by abnormal remodeling of periostin,which affects the differentiation function of osteoclasts in the dental follicle and leads to metabolic imbalance of alveolar bone,resulting in tooth eruption disorders.Whether it is a single or full permanent tooth eruption disorder,once the coronal resistance is removed,the teeth can often erupt normally.
文摘目的探讨24 h动态心电图联合骨膜蛋白(Periostin)、乳糖凝集素-3(Gal-3)对急性心肌梗死(AMI)后恶性心律失常(MA)的预测价值。方法选择2020年1月-2022年12月皖南医学院第一附属医院心血管内科收治的AMI患者367例为研究对象(AMI组),根据是否发生MA分为非MA亚组(n=286)和MA亚组(n=81)。另选取医院同期健康体检志愿者370例为健康对照组。酶联免疫吸附试验(ELISA)检测血清Periostin、Gal-3水平;多因素Logistic回归分析AMI患者发生MA的影响因素;受试者工作特征(ROC)曲线分析Periostin、Gal-3、24 h QT间期变异性(24 h QTV)、24 h正常心房开始除极至心室开始除极时间间期标准差(SDNN)对AMI患者发生MA的预测价值。结果与健康对照组比较,AMI组血清Periostin、Gal-3水平均升高(t/P=59.225/<0.001、33.141/<0.001);与非MA亚组比较,MA亚组血清Periostin、Gal-3水平及低血钾比例均升高(t/χ^(2)/P=7.602/<0.001、6.049/<0.001、15.167/<0.001);与非MA亚组比较,MA亚组SDNN、24 h QTV、24 h连续5 min正常R-R间期标准差(SDANN-index)、24 h连续5 min正常R-R间期标准差均值(SDNN-index)水平降低(t/P=7.029/<0.001、7.401/<0.001、4.281/<0.001、4.721/<0.001);血清Periostin、Gal-3水平升高是AMI患者发生MA的危险因素[OR(95%CI)=2.163(1.416~3.303)、2.450(1.570~3.823)],24 h QTV、SDNN水平升高为保护因素[OR(95%CI)=0.449(0.264~0.764)、0.430(0.223~0.829)];24 h QTV、SDNN、Periostin、Gal-3单独及四项联合预测AMI患者发生MA的AUC分别为0.791、0.719、0.758、0.744、0.901,四者联合预测的AUC高于单独预测(Z/P=3.916/<0.001、6.101/<0.001、4.888/<0.001、5.764/<0.001)。结论AMI患者血清Periostin、Gal-3水平均升高,24 h动态心电图指标联合血清Periostin、Gal-3预测AMI患者发生MA的效能较高。
基金supported by the National Natural Science Foundation of China(No.30800372and30871014)National Basic Research Development Program of China(No.2007CB512004)
文摘心肌纤维化是糖尿病心肌病时心室舒缩功能障碍的主要机制之一。心脏成纤维细胞分泌的一种细胞外基质蛋白periostin与心肌纤维化及间质重塑密切相关。本研究旨在观察高糖对心脏成纤维细胞periostin表达的影响及其相关信号转导机制。在离体培养的成年大鼠心脏成纤维细胞,采用低糖(5.5mmol/L)和高糖(25mmol/L)干预,RT-PCR检测periostinmRNA表达,免疫印迹法检测periostin蛋白表达,荧光法检测细胞内活性氧(reactive oxygen species,ROS)含量。结果显示,高糖刺激12h可使periostin mRNA表达增加117.26%,刺激24h可使其蛋白表达上调93.12%,高糖刺激30min可显著促进心脏成纤维细胞内ROS的产生,蛋白激酶C(protein kinase C,PKC)抑制剂白屈菜红碱可减少高糖诱导的ROS生成增加。高糖诱导的periostin蛋白表达增加可被白屈菜红碱或ROS清除剂N-乙酰-L-半胱氨酸(N-acetylcysteine,NAC)所抑制。高糖刺激30和60min可显著增加磷酸化c-Jun氨基末端激酶(c-jun N-terminal protein kinase,JNK)的含量,应用白屈菜红碱或NAC可抑制高糖诱导的磷酸化JNK含量增加。JNK抑制剂SP600125预孵育明显抑制了高糖诱导的periostin蛋白表达增加。上述结果提示,高糖刺激可促进periostin的mRNA和蛋白表达,激活PKC/ROS/JNK通路介导高糖诱导的periostin表达增加。
