Mycotic pseudo-aneurysm is a rare and potentially fatal sequela of bacteraemia. The injury results in a localized, irreversible dilatation due to destruction of the blood vessel wall by infection, usually with an aero...Mycotic pseudo-aneurysm is a rare and potentially fatal sequela of bacteraemia. The injury results in a localized, irreversible dilatation due to destruction of the blood vessel wall by infection, usually with an aerobic bacterium. Anaerobic bacteria are uncommon. We report an unusual case of a 72-year-old Caucasian man who presented with a rapidly progressive mycotic pseudoaneurysm of the right subclavian artery due to an anaerobic Gram-positive coccus Peptoniphilus asaccharolyticus. Despite optimal treatment, combining antibiotics and surgery, mycotic pseudo-aneurysm has a poor prognosis. When surgery is not possible, antibiotics alone with or without the placement of an endovascular stent have been proposed but in such cases, mortality is very significant. Despite the availability of non-invasive imaging techniques, a strong clinical suspicion is essential for early diagnosis of a mycotic aneurysm. Clinicians must be aware of these important aspects of management.展开更多
Peptostreptococcus anaerobius is an anaerobic bacterium,which has been found selectively en-riched in the fecal and mucosal microbiota of colorectal cancer(CRC)patients.Emerging evidence suggest P.anaerobius may contr...Peptostreptococcus anaerobius is an anaerobic bacterium,which has been found selectively en-riched in the fecal and mucosal microbiota of colorectal cancer(CRC)patients.Emerging evidence suggest P.anaerobius may contribute to the development of CRC in human.In this study,we designed a multi-epitope chimeric vaccine against P.anaerobius PCWBR2,a recently identified adhesin that interacts directly with colon cell lines by bindingα2/β1 integrin frequently overexpressed in human CRC tumors and cell lines.Immunoinformatics tools predicted six cytotoxic T lymphocyte epitopes,five helper T lymphocyte epitopes,and six linear B lymphocyte epitopes.The predicted epitopes were joined with AAY or GPGPG linkers and a previously reported TLR4 agonist was added to the vaccine construct’s N terminal as an adjuvant using EAAAK linkers and the order of epitopes was optimized.Further in silico analysis revealed that the vaccine construct possesses satisfactory antigenicity,allergenicity,solubility,physicochemical properties,adjuvant-TLR4 molecular docking,and immune profile characteristics.Our study provided a promising design for vaccines against P.anaerobius.展开更多
文摘Mycotic pseudo-aneurysm is a rare and potentially fatal sequela of bacteraemia. The injury results in a localized, irreversible dilatation due to destruction of the blood vessel wall by infection, usually with an aerobic bacterium. Anaerobic bacteria are uncommon. We report an unusual case of a 72-year-old Caucasian man who presented with a rapidly progressive mycotic pseudoaneurysm of the right subclavian artery due to an anaerobic Gram-positive coccus Peptoniphilus asaccharolyticus. Despite optimal treatment, combining antibiotics and surgery, mycotic pseudo-aneurysm has a poor prognosis. When surgery is not possible, antibiotics alone with or without the placement of an endovascular stent have been proposed but in such cases, mortality is very significant. Despite the availability of non-invasive imaging techniques, a strong clinical suspicion is essential for early diagnosis of a mycotic aneurysm. Clinicians must be aware of these important aspects of management.
基金supported by the National Key Research and Development Program of China(Grant No.2020YFA0907800)the National Natural Science Foundation of China(Grant No.32000096)+2 种基金the Shenzhen Science and Technology Innovation Program(KQTD20200820145822023)the Program of Shenzhen Key Laboratory of Systems Medicine for Inflammatory Diseases(ZDSYS20220606100803007)the Foshan Science and Technology Innovation Program(2120001010795).
文摘Peptostreptococcus anaerobius is an anaerobic bacterium,which has been found selectively en-riched in the fecal and mucosal microbiota of colorectal cancer(CRC)patients.Emerging evidence suggest P.anaerobius may contribute to the development of CRC in human.In this study,we designed a multi-epitope chimeric vaccine against P.anaerobius PCWBR2,a recently identified adhesin that interacts directly with colon cell lines by bindingα2/β1 integrin frequently overexpressed in human CRC tumors and cell lines.Immunoinformatics tools predicted six cytotoxic T lymphocyte epitopes,five helper T lymphocyte epitopes,and six linear B lymphocyte epitopes.The predicted epitopes were joined with AAY or GPGPG linkers and a previously reported TLR4 agonist was added to the vaccine construct’s N terminal as an adjuvant using EAAAK linkers and the order of epitopes was optimized.Further in silico analysis revealed that the vaccine construct possesses satisfactory antigenicity,allergenicity,solubility,physicochemical properties,adjuvant-TLR4 molecular docking,and immune profile characteristics.Our study provided a promising design for vaccines against P.anaerobius.
