目的制备超顺磁性硫酸链霉素-聚乳酸-聚乙二醇(PELA)微球(superparamagnetic chitosan streptomycin PELA micro-spheres,spCSPM),研究此微球的特性,并对其在振荡磁场作用下体外药物释放规律进行研究。方法用化学共沉淀法合成纳米超顺磁...目的制备超顺磁性硫酸链霉素-聚乳酸-聚乙二醇(PELA)微球(superparamagnetic chitosan streptomycin PELA micro-spheres,spCSPM),研究此微球的特性,并对其在振荡磁场作用下体外药物释放规律进行研究。方法用化学共沉淀法合成纳米超顺磁Fe304壳聚糖纳米粒(superparamagnetic chitosan Fe3O4 nanospheres,spFCN),再用双乳化(W/O/W)溶剂蒸发法制备spCSPM。将spCSPM混合入兔血中形成血凝块,在37℃模拟体液中进行体外药物溶出试验,用振荡磁场干预,用酶联免疫法(ELISA)检测硫酸链霉素的释放量。结果振荡磁场能够增加spCSPM血凝块中链霉素释放速率,与非磁性的壳聚糖聚乳酸-聚乙二醇(PELA)微球(chitosan streptomycin PELA microspheres,CSPM)相比,26d时使药物释放量提高3倍左右。结论spCSPM具有药物缓释功能,振荡磁场可重复性增加体系中药物的溶出,此体系药物缓释周期超过三周。展开更多
AIM: To prepare poly (D,L-lactide)-polyethylene glycol copolymer (PELA) microspheres loaded H.pylorilysates or CyStografin and observe their targeting in gastrointestinal mucous membrane or analyze the mucosal immune ...AIM: To prepare poly (D,L-lactide)-polyethylene glycol copolymer (PELA) microspheres loaded H.pylorilysates or CyStografin and observe their targeting in gastrointestinal mucous membrane or analyze the mucosal immune responses by oral administration. METHODS: PELA microspheres loaded H. Pylorilysates or Cystografin were preparedby double emulsion evaporation method. Their distribution in gastrointestinal mucous membrane was observed by CT. Balb/c mice orally immunized in mucosal immune responses, whose antibody production in salivary and gut washing and antibody secreting cells in Peyer's patches (PP) were estimated by ELISA and ELISPOT, respectively. The microspheres' physical properties, such as particle size, protein level and morphology were investigated.RESULTS: All prepared microspheres were found to have a smooth surface morphology from 3.20-4.05 μm in diameter and high encapsulation efficiency from 74.9-82.2 %. No significant correlation in their physical properties was shown, depending on their molecular weight at the similar composition ratio. Immunization with all types of PELA-Hp microspheres elevated the saliva sIgA level at week 3 by approximately 3-4 times that with soluble antigen, which was greatly enhanced after boosting. At one week after last immunization with all types of PELA-Hp microspheres (week 8), the specific sIgA-ASCs, IgG-ASCs and stgA in salivary rose obviously. In intestinal Peyer's patches, the specific sIgA-ASCs were 5.92-6.98× 104/ml cell and IgG-ASCs were 3.47-4.02 × 104/ml cell, about 5-9 times higher than those with soluble antigen (P＜0.01). ASCs in intestine were more than those in stomach and the majority of the ASCs were sIgA-ASCs. The sIgA in gut washing fluid was 1.62-1.85 OD, about 3-6 times tthat of those with soluble antigen. There were significant differences of the ASCs and sIgA in gut washing fluid as compared with those of PBS and MS-0 (P＜0.05). There appeared to be good correlation between sIgA level in gut washing fluid and sIgA-ASCs in intestinal Peyer's patches.CONCLUSION: PELA microspheres may be used as vehicle to delivery antigen and adjuvant in designing oral vaccination.展开更多
应用Ewen’s改良多聚醛品红染色方法,对雌性白蜡虫(Ericerus pela Cha-vannes)成虫神经分泌系统的形态及组织学进行了研究.确定了在前脑中存在有两群属于“A”型神经分泌细胞.愈合腹神经节中亦有分散的“A”型神经分泌细胞.脑神经分泌...应用Ewen’s改良多聚醛品红染色方法,对雌性白蜡虫(Ericerus pela Cha-vannes)成虫神经分泌系统的形态及组织学进行了研究.确定了在前脑中存在有两群属于“A”型神经分泌细胞.愈合腹神经节中亦有分散的“A”型神经分泌细胞.脑神经分泌细胞中的神经分泌粒通过其轴突传送到心侧体中释放.心侧体为神经血器官.虫体在进入成体阶段后到产卵期间,其脑中的神经分泌细胞在形态及染色性上都没有明显差异.展开更多
文摘AIM: To prepare poly (D,L-lactide)-polyethylene glycol copolymer (PELA) microspheres loaded H.pylorilysates or CyStografin and observe their targeting in gastrointestinal mucous membrane or analyze the mucosal immune responses by oral administration. METHODS: PELA microspheres loaded H. Pylorilysates or Cystografin were preparedby double emulsion evaporation method. Their distribution in gastrointestinal mucous membrane was observed by CT. Balb/c mice orally immunized in mucosal immune responses, whose antibody production in salivary and gut washing and antibody secreting cells in Peyer's patches (PP) were estimated by ELISA and ELISPOT, respectively. The microspheres' physical properties, such as particle size, protein level and morphology were investigated.RESULTS: All prepared microspheres were found to have a smooth surface morphology from 3.20-4.05 μm in diameter and high encapsulation efficiency from 74.9-82.2 %. No significant correlation in their physical properties was shown, depending on their molecular weight at the similar composition ratio. Immunization with all types of PELA-Hp microspheres elevated the saliva sIgA level at week 3 by approximately 3-4 times that with soluble antigen, which was greatly enhanced after boosting. At one week after last immunization with all types of PELA-Hp microspheres (week 8), the specific sIgA-ASCs, IgG-ASCs and stgA in salivary rose obviously. In intestinal Peyer's patches, the specific sIgA-ASCs were 5.92-6.98× 104/ml cell and IgG-ASCs were 3.47-4.02 × 104/ml cell, about 5-9 times higher than those with soluble antigen (P＜0.01). ASCs in intestine were more than those in stomach and the majority of the ASCs were sIgA-ASCs. The sIgA in gut washing fluid was 1.62-1.85 OD, about 3-6 times tthat of those with soluble antigen. There were significant differences of the ASCs and sIgA in gut washing fluid as compared with those of PBS and MS-0 (P＜0.05). There appeared to be good correlation between sIgA level in gut washing fluid and sIgA-ASCs in intestinal Peyer's patches.CONCLUSION: PELA microspheres may be used as vehicle to delivery antigen and adjuvant in designing oral vaccination.
文摘应用Ewen’s改良多聚醛品红染色方法,对雌性白蜡虫(Ericerus pela Cha-vannes)成虫神经分泌系统的形态及组织学进行了研究.确定了在前脑中存在有两群属于“A”型神经分泌细胞.愈合腹神经节中亦有分散的“A”型神经分泌细胞.脑神经分泌细胞中的神经分泌粒通过其轴突传送到心侧体中释放.心侧体为神经血器官.虫体在进入成体阶段后到产卵期间,其脑中的神经分泌细胞在形态及染色性上都没有明显差异.
