Prime editing is a versatile CRISPR/Cas-based precise genome-editing technique for crop breeding.Four new types of prime editors(PEs)named PE6a–d were recently generated using evolved and engineered reverse transcrip...Prime editing is a versatile CRISPR/Cas-based precise genome-editing technique for crop breeding.Four new types of prime editors(PEs)named PE6a–d were recently generated using evolved and engineered reverse transcriptase(RT)variants from three different sources.In this study,we tested the editing efficiencies of four PE6 variants and two additional PE6 constructs with double-RT modules in transgenic rice(Oryza sativa)plants.PE6c,with an evolved and engineered RT variant from the yeast Tf1 retrotransposon,yielded the highest prime-editing efficiency.The average fold change in the editing efficiency of PE6c compared with PEmax exceeded 3.5 across 18 agronomically important target sites from 15 genes.We also demonstrated the feasibility of using two RT modules to improve prime-editing efficiency.Our results suggest that PE6c or its derivatives would be an excellent choice for prime editing in monocot plants.In addition,our findings have laid a foundation for prime-editing-based breeding of rice varieties with enhanced agronomically important traits.展开更多
由于IPv4网络地址匮乏,由IPv4向IPv6过渡是网络发展的必然趋势。文章针对目前电力数据网络重点考虑的3种IPv6过渡技术(双栈、IPv6 over IPv4隧道和6PE/6VPE),结合电力数据网络实际部署情况,对其分别进行了路由、业务转发、Qo S、地址获...由于IPv4网络地址匮乏,由IPv4向IPv6过渡是网络发展的必然趋势。文章针对目前电力数据网络重点考虑的3种IPv6过渡技术(双栈、IPv6 over IPv4隧道和6PE/6VPE),结合电力数据网络实际部署情况,对其分别进行了路由、业务转发、Qo S、地址获取和可靠性等方面的研究和仿真分析,验证了这3种过渡技术适合目前电力数据网络从IPv4向IPv6过渡演进的要求,可以为电力数据网络向IPv6的过渡演进提供依据。展开更多
如何在IPv6 over MPLS网络中提供端到端的服务质量?针对这个问题介绍了一个6PE-DiffServ解决方案(6PE,IPv6 Provider Edge Routers)。该方案使用6PE技术进行组网,通过统一的DiffServ策略的部署和BB-6PE(Bandwidth Broker for 6PE netwo...如何在IPv6 over MPLS网络中提供端到端的服务质量?针对这个问题介绍了一个6PE-DiffServ解决方案(6PE,IPv6 Provider Edge Routers)。该方案使用6PE技术进行组网,通过统一的DiffServ策略的部署和BB-6PE(Bandwidth Broker for 6PE network)的集中管理,给由IPv4向IPv6过渡的网络提供DiffServ服务。6PE-DiffServ方案的可行性和有效性在CNGI-QoS实验网中得到了验证。展开更多
基金supported by grants from the National Key Research and Development Program of China (2023YFD1202905)the National Natural Science Foundation of China (U19A2022)
文摘Prime editing is a versatile CRISPR/Cas-based precise genome-editing technique for crop breeding.Four new types of prime editors(PEs)named PE6a–d were recently generated using evolved and engineered reverse transcriptase(RT)variants from three different sources.In this study,we tested the editing efficiencies of four PE6 variants and two additional PE6 constructs with double-RT modules in transgenic rice(Oryza sativa)plants.PE6c,with an evolved and engineered RT variant from the yeast Tf1 retrotransposon,yielded the highest prime-editing efficiency.The average fold change in the editing efficiency of PE6c compared with PEmax exceeded 3.5 across 18 agronomically important target sites from 15 genes.We also demonstrated the feasibility of using two RT modules to improve prime-editing efficiency.Our results suggest that PE6c or its derivatives would be an excellent choice for prime editing in monocot plants.In addition,our findings have laid a foundation for prime-editing-based breeding of rice varieties with enhanced agronomically important traits.
文摘由于IPv4网络地址匮乏,由IPv4向IPv6过渡是网络发展的必然趋势。文章针对目前电力数据网络重点考虑的3种IPv6过渡技术(双栈、IPv6 over IPv4隧道和6PE/6VPE),结合电力数据网络实际部署情况,对其分别进行了路由、业务转发、Qo S、地址获取和可靠性等方面的研究和仿真分析,验证了这3种过渡技术适合目前电力数据网络从IPv4向IPv6过渡演进的要求,可以为电力数据网络向IPv6的过渡演进提供依据。
文摘如何在IPv6 over MPLS网络中提供端到端的服务质量?针对这个问题介绍了一个6PE-DiffServ解决方案(6PE,IPv6 Provider Edge Routers)。该方案使用6PE技术进行组网,通过统一的DiffServ策略的部署和BB-6PE(Bandwidth Broker for 6PE network)的集中管理,给由IPv4向IPv6过渡的网络提供DiffServ服务。6PE-DiffServ方案的可行性和有效性在CNGI-QoS实验网中得到了验证。
