In environmental biosafety assessments of glyphosate-tolerant crops,it is essential to evaluate the effects of cultivating these crops and applying glyphosate on the microbial community in the rhizosphere soil,which p...In environmental biosafety assessments of glyphosate-tolerant crops,it is essential to evaluate the effects of cultivating these crops and applying glyphosate on the microbial community in the rhizosphere soil,which play a critical role in maintaining soil health,plant growth,and crop productivity.Maize(Zea mays)line GG2 was previously generated by transforming wild-type maize with the gat and gr79-epsps genes,endowing GG2 with both active and passive resistance to glyphosate.However,the ecological risk of introducing these two new glyphosate-tolerance genes into maize,as well as glyphosate treatment,to rhizosphere microorganisms remain unclear.In this study,we used high-throughput sequencing to analyze the diversity and composition of the bacterial and fungal communities in the rhizosphere soil around biotech maize GG2,with(GG2-H)and without glyphosate treatment(GG2-N),compared with the near-isogenic,non-biotech maize line ZD958 at seven stages of growth.The structure and diversity of the bacterial and fungal communities of GG2-H were similar to those of ZD958,whereas glyphosate treatment had temporary effects on bacterial and fungal diversity and richness.The differences in the bacterial and fungal communities were associated with changes in soil properties such as pH,available phosphorus and organic matter,and seasonal changes.These factors,rather than maize lines,made the greatest contributions to the shifts in bacterial and fungal community structure.This study provides a comprehensive analysis of the effects of biotech crop cultivation,glyphosate treatment,soil physicochemical properties of soil,and maize growth stages on soil microbial communities,offering valuable insights for the large-scale adoption of biotech crops in China.展开更多
There are five hot springs named separately SHANGT,ZHONGT,WENT,XIAT and SHENT in Lushan County,Pingdingshan City,Henan Province.The archaeal diversity of the five hot springs was investigated and analyzed.16 S rD NA v...There are five hot springs named separately SHANGT,ZHONGT,WENT,XIAT and SHENT in Lushan County,Pingdingshan City,Henan Province.The archaeal diversity of the five hot springs was investigated and analyzed.16 S rD NA v4-5 genes of archaea were sequenced by Illumina Hisiq2500 PE250 technology.The numbers of sample sequences and OTUs were sorted by using Uparse,Mothur,SILVA,MUSCLE and Qiime software.Finally,the archaeal abundance and diversity in the five hot springs were analyzed.87993,67326,44400,48611 and 48495 effective reads have been obtained from SHANGT,ZHONGT,WENT,XIAT and SHENT respectively,and 520,280,678,451 and 437 OTUs have been acquired.The analysis of archaeal classification showed that the archaea from the five hot springs could be classified into 11 phyla,in which Thaumarchaeota accounted for 95.25%and was the dominant archaea.In the genera level,the dominant archaea of the SHANGT,ZHONGT and WENT was Candidatus-Nitrosocaldus,and unidentified-Thaumarchaeota was the dominant archaea in XIAT and SHENT.The results showed that the five hot springs in Lushan are rich in microbial resources.The research provided basic data for further research and utilization of the archaea resources in the five hot springs of Lushan.展开更多
基金supported by the Nanfan Special Project(Grant No.YBXM2318)the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(CAAS-ZDRW202402)the Biological Breeding-National Science and Technology Major Project(2022ZD0400602).
文摘In environmental biosafety assessments of glyphosate-tolerant crops,it is essential to evaluate the effects of cultivating these crops and applying glyphosate on the microbial community in the rhizosphere soil,which play a critical role in maintaining soil health,plant growth,and crop productivity.Maize(Zea mays)line GG2 was previously generated by transforming wild-type maize with the gat and gr79-epsps genes,endowing GG2 with both active and passive resistance to glyphosate.However,the ecological risk of introducing these two new glyphosate-tolerance genes into maize,as well as glyphosate treatment,to rhizosphere microorganisms remain unclear.In this study,we used high-throughput sequencing to analyze the diversity and composition of the bacterial and fungal communities in the rhizosphere soil around biotech maize GG2,with(GG2-H)and without glyphosate treatment(GG2-N),compared with the near-isogenic,non-biotech maize line ZD958 at seven stages of growth.The structure and diversity of the bacterial and fungal communities of GG2-H were similar to those of ZD958,whereas glyphosate treatment had temporary effects on bacterial and fungal diversity and richness.The differences in the bacterial and fungal communities were associated with changes in soil properties such as pH,available phosphorus and organic matter,and seasonal changes.These factors,rather than maize lines,made the greatest contributions to the shifts in bacterial and fungal community structure.This study provides a comprehensive analysis of the effects of biotech crop cultivation,glyphosate treatment,soil physicochemical properties of soil,and maize growth stages on soil microbial communities,offering valuable insights for the large-scale adoption of biotech crops in China.
基金Supported by the Key Scientific Research Project of Colleges and Universities in Henan Province(16B180004)Science and Technology Public Relations Project from Department of Science and Technology in Henan Province(172102310211,172102310529)+1 种基金Scientific Research Start-up Foundation of High-level Talents in Pingdingshan University(PXY-BSQD-2015006)National Natural Fund Cultivation Project of Pingdingshan University(PXYPYJJ2016004)
文摘There are five hot springs named separately SHANGT,ZHONGT,WENT,XIAT and SHENT in Lushan County,Pingdingshan City,Henan Province.The archaeal diversity of the five hot springs was investigated and analyzed.16 S rD NA v4-5 genes of archaea were sequenced by Illumina Hisiq2500 PE250 technology.The numbers of sample sequences and OTUs were sorted by using Uparse,Mothur,SILVA,MUSCLE and Qiime software.Finally,the archaeal abundance and diversity in the five hot springs were analyzed.87993,67326,44400,48611 and 48495 effective reads have been obtained from SHANGT,ZHONGT,WENT,XIAT and SHENT respectively,and 520,280,678,451 and 437 OTUs have been acquired.The analysis of archaeal classification showed that the archaea from the five hot springs could be classified into 11 phyla,in which Thaumarchaeota accounted for 95.25%and was the dominant archaea.In the genera level,the dominant archaea of the SHANGT,ZHONGT and WENT was Candidatus-Nitrosocaldus,and unidentified-Thaumarchaeota was the dominant archaea in XIAT and SHENT.The results showed that the five hot springs in Lushan are rich in microbial resources.The research provided basic data for further research and utilization of the archaea resources in the five hot springs of Lushan.
