Fusobacterium nucleatum(F.nucleatum)is an early pathogenic colonizer in periodontitis,but the host response to infection with this pathogen remains unclear.In this study,we built an F.nucleatum infectious model with h...Fusobacterium nucleatum(F.nucleatum)is an early pathogenic colonizer in periodontitis,but the host response to infection with this pathogen remains unclear.In this study,we built an F.nucleatum infectious model with human periodontal ligament stem cells(PDLSCs)and showed that F.nucleatum could inhibit proliferation,and facilitate apoptosis,ferroptosis,and inflammatory cytokine production in a dose-dependent manner.The F.nucleatum adhesin Fad A acted as a proinflammatory virulence factor and increased the expression of interleukin(IL)-1β,IL-6 and IL-8.Further study showed that Fad A could bind with PEBP1 to activate the Raf1-MAPK and IKK-NF-κB signaling pathways.Time-course RNA-sequencing analyses showed the cascade of gene activation process in PDLSCs with increasing durations of F.nucleatum infection.NFκB1 and NFκB2 upregulated after 3 h of F.nucleatum-infection,and the inflammatory-related genes in the NF-κB signaling pathway were serially elevated with time.Using computational drug repositioning analysis,we predicted and validated that two potential drugs(piperlongumine and fisetin)could attenuate the negative effects of F.nucleatum-infection.Collectively,this study unveils the potential pathogenic mechanisms of F.nucleatum and the host inflammatory response at the early stage of F.nucleatum infection.展开更多
The periodontal ligament(PDL)plays a crucial role in transmitting and dispersing occlusal force,acting as mechanoreceptor for muscle activity during chewing,as well as mediating orthodontic tooth movement.It transform...The periodontal ligament(PDL)plays a crucial role in transmitting and dispersing occlusal force,acting as mechanoreceptor for muscle activity during chewing,as well as mediating orthodontic tooth movement.It transforms mechanical stimuli into biological signals,influencing alveolar bone remodeling.Recent research has delved deeper into the biological and mechanical aspects of PDL,emphasizing the importance of understanding its structure and mechanical properties comprehensively.This review focuses on the latest findings concerning both macro-and micro-structural aspects of the PDL,highlighting its mechanical characteristics and factors that influence them.Moreover,it explores the mechanotransduction mechanisms of PDL cells under mechanical forces.Structure-mechanics-mechanotransduction interplay in PDL has been integrated ultimately.By providing an up-to-date overview of our understanding on PDL at various scales,this study lays the foundation for further exploration into PDL-related biomechanics and mechanobiology.展开更多
In the previous research,when the economic factor forecasting solution of Urbanization was used into practice,the users almost always ignored the effects of the lagged economic development factors.The accuracy of the ...In the previous research,when the economic factor forecasting solution of Urbanization was used into practice,the users almost always ignored the effects of the lagged economic development factors.The accuracy of the forecasting should be decreased seriously by this way.In this paper,taking Gansu province as an example,the authors try to improve the forecasting accuracy on the basis of the unrestricted PDL(Polynomial Distributed Lag)Models.In the context,the ARIMA Models and CPPS software are also applied to finish the forecasting more accurately.展开更多
基金foundation support of the National Natural Science Foundation of China(Grant No.82071122)the Program of Taishan Young from Shandong Province+3 种基金Major Innovation Projects in Shandong Province(No.2021SFGC0502)Oral Microbiome Innovation Team of Young Scientist Project of Shandong Province(Grant No.2020KJK001)and Jinan City(2021GXRC021)The National High-level Young Scientist Project Foundation(2019)Excellent Young Scientist Foundation of Shandong Province(Grant No.ZR202102230369)。
文摘Fusobacterium nucleatum(F.nucleatum)is an early pathogenic colonizer in periodontitis,but the host response to infection with this pathogen remains unclear.In this study,we built an F.nucleatum infectious model with human periodontal ligament stem cells(PDLSCs)and showed that F.nucleatum could inhibit proliferation,and facilitate apoptosis,ferroptosis,and inflammatory cytokine production in a dose-dependent manner.The F.nucleatum adhesin Fad A acted as a proinflammatory virulence factor and increased the expression of interleukin(IL)-1β,IL-6 and IL-8.Further study showed that Fad A could bind with PEBP1 to activate the Raf1-MAPK and IKK-NF-κB signaling pathways.Time-course RNA-sequencing analyses showed the cascade of gene activation process in PDLSCs with increasing durations of F.nucleatum infection.NFκB1 and NFκB2 upregulated after 3 h of F.nucleatum-infection,and the inflammatory-related genes in the NF-κB signaling pathway were serially elevated with time.Using computational drug repositioning analysis,we predicted and validated that two potential drugs(piperlongumine and fisetin)could attenuate the negative effects of F.nucleatum-infection.Collectively,this study unveils the potential pathogenic mechanisms of F.nucleatum and the host inflammatory response at the early stage of F.nucleatum infection.
基金supported by the National Natural Science Foundation of China(32271416)Sichuan Provincial Science and Technology program(2022YFQ0002).
文摘The periodontal ligament(PDL)plays a crucial role in transmitting and dispersing occlusal force,acting as mechanoreceptor for muscle activity during chewing,as well as mediating orthodontic tooth movement.It transforms mechanical stimuli into biological signals,influencing alveolar bone remodeling.Recent research has delved deeper into the biological and mechanical aspects of PDL,emphasizing the importance of understanding its structure and mechanical properties comprehensively.This review focuses on the latest findings concerning both macro-and micro-structural aspects of the PDL,highlighting its mechanical characteristics and factors that influence them.Moreover,it explores the mechanotransduction mechanisms of PDL cells under mechanical forces.Structure-mechanics-mechanotransduction interplay in PDL has been integrated ultimately.By providing an up-to-date overview of our understanding on PDL at various scales,this study lays the foundation for further exploration into PDL-related biomechanics and mechanobiology.
文摘In the previous research,when the economic factor forecasting solution of Urbanization was used into practice,the users almost always ignored the effects of the lagged economic development factors.The accuracy of the forecasting should be decreased seriously by this way.In this paper,taking Gansu province as an example,the authors try to improve the forecasting accuracy on the basis of the unrestricted PDL(Polynomial Distributed Lag)Models.In the context,the ARIMA Models and CPPS software are also applied to finish the forecasting more accurately.
