Pacific white shrimp(Penaeus vannamei)is a globally important aquaculture species,prized for its adaptability,high yield,and economic value.Growth traits are key determinants of aquaculture profitability,making it ess...Pacific white shrimp(Penaeus vannamei)is a globally important aquaculture species,prized for its adaptability,high yield,and economic value.Growth traits are key determinants of aquaculture profitability,making it essential to understand their genetic basis.In this study,we performed whole-genome sequencing of 996 individual P.vannamei,identifying 2,706,353 high-quality SNPs through genotyping and imputation.A genome-wide association study(GWAS)revealed six quantitative trait loci(QTLs)on linkage group(LG)2,25,26,and 37,with 30 SNPs significantly associated with body length.The most significant associations were located on LG37,encompassing PDE11A,SAAL,IAO-like,and several other genes potentially involved in molting and growth regulation.Functional validation through RNA interference demonstrated that silencing PDE11A and SAAL significantly reduced growth,confirming their critical role in growth modulation.This integrative approach,combining a GWAS with functional genomics,sheds light on the molecular mechanisms governing in P.vannamei.The identification and validation of PDE11A and SAAL as key growth-related genes offer valuable insights for developing selective breeding strategies to improve growth traits in aquaculture species.展开更多
基金funded by the National Key Research and Development Program of China(2022YFD2400201)Project of Sanya Yazhou Bay Science and Technology City(SKJC-2022-PTDX-025)+4 种基金Key R&D Project of Hainan Province(ZDYF2023XDNY176)Hainan Yazhou Bay Seed industry laboratory‘JBGS’project(B24YQ0009)Major Science and Technology Program of Hainan Province(ZDKJ2021017)the open competition program of top ten critical priorities of Agricultural Science and Technology Innovation for the 14th Five-Year Plan of Guangdong Province(2022SDZG01)Hebei Province Innovation Capacity Enhancement Program Project(225A6302D).
文摘Pacific white shrimp(Penaeus vannamei)is a globally important aquaculture species,prized for its adaptability,high yield,and economic value.Growth traits are key determinants of aquaculture profitability,making it essential to understand their genetic basis.In this study,we performed whole-genome sequencing of 996 individual P.vannamei,identifying 2,706,353 high-quality SNPs through genotyping and imputation.A genome-wide association study(GWAS)revealed six quantitative trait loci(QTLs)on linkage group(LG)2,25,26,and 37,with 30 SNPs significantly associated with body length.The most significant associations were located on LG37,encompassing PDE11A,SAAL,IAO-like,and several other genes potentially involved in molting and growth regulation.Functional validation through RNA interference demonstrated that silencing PDE11A and SAAL significantly reduced growth,confirming their critical role in growth modulation.This integrative approach,combining a GWAS with functional genomics,sheds light on the molecular mechanisms governing in P.vannamei.The identification and validation of PDE11A and SAAL as key growth-related genes offer valuable insights for developing selective breeding strategies to improve growth traits in aquaculture species.
