In this paper, the theory of plausible and paradoxical reasoning of Dezert- Smarandache (DSmT) is used to take into account the paradoxical charac-ter through the intersections of vegetation, aquatic and mineral surfa...In this paper, the theory of plausible and paradoxical reasoning of Dezert- Smarandache (DSmT) is used to take into account the paradoxical charac-ter through the intersections of vegetation, aquatic and mineral surfaces. In order to do this, we developed a classification model of pixels by aggregating information using the DSmT theory based on the PCR5 rule using the ∩NDVI, ∩MNDWI and ∩NDBaI spectral indices obtained from the ASTER satellite images. On the qualitative level, the model produced three simple classes for certain knowledge (E, V, M) and eight composite classes including two union classes characterizing partial ignorance ({E,V}, {M,V}) and six classes of intersection of which three classes of simple intersection (E∩V, M∩V, E∩M) and three classes of composite intersection (E∩{M,V}, M∩{E,V}, V∩{E,M}), which represent paradoxes. This model was validated with an average rate of 93.34% for the well-classified pixels and a compliance rate of the entities in the field of 96.37%. Thus, the model 1 retained provides 84.98% for the simple classes against 15.02% for the composite classes.展开更多
Reference genes, stably expressing in different tissues and cells, are commonly used as the references in expression analysis. Selecting the optimum reference gene is crucial to the success of experiments. In this stu...Reference genes, stably expressing in different tissues and cells, are commonly used as the references in expression analysis. Selecting the optimum reference gene is crucial to the success of experiments. In this study, the expression stabilities of nine common reference genes, including ACT2, 18 S r RNA, APRT, EF-1α, RNA POL II, TUBα, TUBβ, GAPDH and TLF of Agropyron mongolicum, were studied under drought condition. Among them, 18 S r RNA was found to be the most optimum reference gene under drought stress by the analyzing of ge Norm and Norm Finder software. Quantitative expression levels of P5 CS using 18 S r RNA as the reference gene, and proline contents under drought stress in A. mongolicum were further operated, and we found the expression level of P5 CS gene and proline content had a significantly positive relationship(R^2=0.7763, P〈0.05). This study established and validated 18 S r RNA as the reference genes in A. mongolicum under drought stress, providing a powerful tool for the quantitative expression analysis of drought genes in A. mongolicum.展开更多
文摘In this paper, the theory of plausible and paradoxical reasoning of Dezert- Smarandache (DSmT) is used to take into account the paradoxical charac-ter through the intersections of vegetation, aquatic and mineral surfaces. In order to do this, we developed a classification model of pixels by aggregating information using the DSmT theory based on the PCR5 rule using the ∩NDVI, ∩MNDWI and ∩NDBaI spectral indices obtained from the ASTER satellite images. On the qualitative level, the model produced three simple classes for certain knowledge (E, V, M) and eight composite classes including two union classes characterizing partial ignorance ({E,V}, {M,V}) and six classes of intersection of which three classes of simple intersection (E∩V, M∩V, E∩M) and three classes of composite intersection (E∩{M,V}, M∩{E,V}, V∩{E,M}), which represent paradoxes. This model was validated with an average rate of 93.34% for the well-classified pixels and a compliance rate of the entities in the field of 96.37%. Thus, the model 1 retained provides 84.98% for the simple classes against 15.02% for the composite classes.
基金supported by the Western Light Project of Chinese Academy of Sciencesthe National Natural Science Foundation of China(31060057)the National Natural Science Foundation of Inner Mongolia,China(2015MS0305)
文摘Reference genes, stably expressing in different tissues and cells, are commonly used as the references in expression analysis. Selecting the optimum reference gene is crucial to the success of experiments. In this study, the expression stabilities of nine common reference genes, including ACT2, 18 S r RNA, APRT, EF-1α, RNA POL II, TUBα, TUBβ, GAPDH and TLF of Agropyron mongolicum, were studied under drought condition. Among them, 18 S r RNA was found to be the most optimum reference gene under drought stress by the analyzing of ge Norm and Norm Finder software. Quantitative expression levels of P5 CS using 18 S r RNA as the reference gene, and proline contents under drought stress in A. mongolicum were further operated, and we found the expression level of P5 CS gene and proline content had a significantly positive relationship(R^2=0.7763, P〈0.05). This study established and validated 18 S r RNA as the reference genes in A. mongolicum under drought stress, providing a powerful tool for the quantitative expression analysis of drought genes in A. mongolicum.