Poly(ADP-ribose)polymerase-1(PARP-1)can exacerbate ischemic brain injury and lessen ischemic neuronal death,which may be associated with PARP-1 polymorphisms.The present study investigated human PARP-1 gene polymorphi...Poly(ADP-ribose)polymerase-1(PARP-1)can exacerbate ischemic brain injury and lessen ischemic neuronal death,which may be associated with PARP-1 polymorphisms.The present study investigated human PARP-1 gene polymorphisms in various Chinese nationalities,the results of which could potentially help in the treatment and prevention of neurologic diseases.Genetic polymorphisms of seven exons in the PARP-1 gene,in 898 Chinese Han,Buyi,Shui,Miao,and Zhuang subjects,were investigated by PCR-single-strand conformation polymorphism.A single-strand conformation polymorphism variant in exons 12,13,16,and 17 of the PARP-1 gene was identified in 148 people,with two stationary bands showing three degenerative single strands.Results showed that the PARP-1 gene polymorphisms exist in various nationalities,and may act as a biomarker for susceptibility to disease.展开更多
PCR-single-strand conformation polymorphism (SSCP) and Southern blotting tech-niques were adopted to investigate microbial community dynamics in a sulfate-reducing bioreactor caused by decreasing influent alkalinity. ...PCR-single-strand conformation polymorphism (SSCP) and Southern blotting tech-niques were adopted to investigate microbial community dynamics in a sulfate-reducing bioreactor caused by decreasing influent alkalinity. Experimental results indicated that the sulfate-removal rate approached 87% in 25 d under the conditions of influent alkalinity of 4000 mg/L (as CaCO3) and sul-fate-loading rate of 4.8 g/(L·d), which indicated that the bioreactor started up successfully. The analy-sis of microbial community structure in this stage showed that Lactococcus sp., Anaerofilum sp. and Kluyvera sp. were dominant populations. It was found that when influent alkalinity reduced to 1000 mg/L, sulfate-removal rate decreased rapidly to 35% in 3 d. Then influent alkalinity was increased to 3000 mg/L, the sulfate-removal rate rose to 55%. Under these conditions, the populations of Dysgo-nomonas sp., Sporobacte sp., Obesumbacterium sp. and Clostridium sp. got to rich, which predomi-nated in the community together with Lactococcus sp., Anaerofilum sp. and Kluyvera sp. However, when the alkalinity was decreased to 1500 mg/L, the sulfate-removal rate rose to and kept stable at 70% and populations of Dysgonomonas sp., Sporobacter sp. and Obesumbacterium sp. died out, while some strains of Desulfovibrio sp. and Clostridium sp. increased in concentration. In order to determine the minimum alkalinity value that the system could tolerate, the influent alkalinity was de-creased from 1500 to 400 mg/L secondly. This resulted in the sulfate-removal rate, pH value and ef-fluent alkalinity dropping quickly. The amount of Petrotoga sp., Prevotella sp., Kluyvera sp. and Neisseria sp. reduced obviously. The result data from Southern blotting indicated that the amount of sulfate-reducing bacteria (SRBs) decreased with influent alkalinity dropping. Analysis of the microbial community structure and diversity showed that the SRBs populations were very abundant in the in-oculated activated sludge and the alkalinity decrease caused the reduction of the populations noted. Most of resident populations in the bioreactor were fermentative acidogenic bacteria (FABs), among which the phylum Firmicute was in the majority, but SRBs were very few. This community structure demonstrates the cooperation between SRBs and FABs, which sustains the system’s high sul-fate-removal and operation stability.展开更多
基金the National Natural Science Foundation of China,No.30972500the Natural Science Foundation of Guangdong Province,No.7301507
文摘Poly(ADP-ribose)polymerase-1(PARP-1)can exacerbate ischemic brain injury and lessen ischemic neuronal death,which may be associated with PARP-1 polymorphisms.The present study investigated human PARP-1 gene polymorphisms in various Chinese nationalities,the results of which could potentially help in the treatment and prevention of neurologic diseases.Genetic polymorphisms of seven exons in the PARP-1 gene,in 898 Chinese Han,Buyi,Shui,Miao,and Zhuang subjects,were investigated by PCR-single-strand conformation polymorphism.A single-strand conformation polymorphism variant in exons 12,13,16,and 17 of the PARP-1 gene was identified in 148 people,with two stationary bands showing three degenerative single strands.Results showed that the PARP-1 gene polymorphisms exist in various nationalities,and may act as a biomarker for susceptibility to disease.
基金supported by the National Natural Science Foundation of China(Grant No.50208006)National 863 Plan of the Ministry of Science and Technology of China(Grant No.2002AA001036).
文摘PCR-single-strand conformation polymorphism (SSCP) and Southern blotting tech-niques were adopted to investigate microbial community dynamics in a sulfate-reducing bioreactor caused by decreasing influent alkalinity. Experimental results indicated that the sulfate-removal rate approached 87% in 25 d under the conditions of influent alkalinity of 4000 mg/L (as CaCO3) and sul-fate-loading rate of 4.8 g/(L·d), which indicated that the bioreactor started up successfully. The analy-sis of microbial community structure in this stage showed that Lactococcus sp., Anaerofilum sp. and Kluyvera sp. were dominant populations. It was found that when influent alkalinity reduced to 1000 mg/L, sulfate-removal rate decreased rapidly to 35% in 3 d. Then influent alkalinity was increased to 3000 mg/L, the sulfate-removal rate rose to 55%. Under these conditions, the populations of Dysgo-nomonas sp., Sporobacte sp., Obesumbacterium sp. and Clostridium sp. got to rich, which predomi-nated in the community together with Lactococcus sp., Anaerofilum sp. and Kluyvera sp. However, when the alkalinity was decreased to 1500 mg/L, the sulfate-removal rate rose to and kept stable at 70% and populations of Dysgonomonas sp., Sporobacter sp. and Obesumbacterium sp. died out, while some strains of Desulfovibrio sp. and Clostridium sp. increased in concentration. In order to determine the minimum alkalinity value that the system could tolerate, the influent alkalinity was de-creased from 1500 to 400 mg/L secondly. This resulted in the sulfate-removal rate, pH value and ef-fluent alkalinity dropping quickly. The amount of Petrotoga sp., Prevotella sp., Kluyvera sp. and Neisseria sp. reduced obviously. The result data from Southern blotting indicated that the amount of sulfate-reducing bacteria (SRBs) decreased with influent alkalinity dropping. Analysis of the microbial community structure and diversity showed that the SRBs populations were very abundant in the in-oculated activated sludge and the alkalinity decrease caused the reduction of the populations noted. Most of resident populations in the bioreactor were fermentative acidogenic bacteria (FABs), among which the phylum Firmicute was in the majority, but SRBs were very few. This community structure demonstrates the cooperation between SRBs and FABs, which sustains the system’s high sul-fate-removal and operation stability.
