Objective:To screen potential serum differential proteins in insulin resistance(IR)complicated with polycystic ovary syndrome(PCOS)using isobaric tags for relative and absolute quantitation(i TRAQ)combined with ultra-...Objective:To screen potential serum differential proteins in insulin resistance(IR)complicated with polycystic ovary syndrome(PCOS)using isobaric tags for relative and absolute quantitation(i TRAQ)combined with ultra-high performance liquid chromatography-tandem mass spectrometry(LC-MS/MS).Methods:A total of 20 patients diagnosed with PCOS in our hospital were selected,including 10 cases of simple PCOS and 10 cases of PCOS+IR.i TRAQ combined with LC-MS/MS was used for proteomic analysis to identify serum differential proteins.Bioinformatics analyses,including gene ontology(GO),Kyoto encyclopedia of genes and genomes(KEGG),and protein-protein interaction(PPI)analysis,were conducted to understand the biological processes,cellular components,and molecular functions of the differentially expressed proteins detected by the two methods.Results:A total of 454,675 secondary spectra were detected by iTRAQ,with 14,376 matched spectra.Combined with LC-MS/MS,74,386peptides,47,542 unique peptide sequences,and 54,675 proteins were identified.A total of 249 differentially expressed proteins with a fold change≥1.30 or≤0.83 were found,among which 9 had a P-value<0.05.There were 5 up-regulated and 4 down-regulated proteins,namely RPAP3,ALDH6A1,COX20,RASSF3,ALPK2,NANOS1,FAM210A,CHGA,and CGA.These differential proteins were imported into the STRING database for PPI network analysis,which revealed 21 nodes and 69 edges with a PPI enrichment P-value<0.001.KEGG enrichment results included hsa04913(Ovarian steroidogenesis),hsa04024(cAMP signaling pathway),and hsa04080(Neuroactive ligand-receptor interaction).Conclusion:The combination of i TRAQ and LC-MS/MS technologies identified nine differential proteins including CHGA and CGA,which are mainly enriched in pathways related to ovarian steroidogenesis,c AMP signaling,and neuroactive ligand-receptor interaction.This combination provides a powerful platform for exploring the pathogenesis of IR complicated with PCOS and discovering new biomarkers.展开更多
你是否经常听到身边的朋友抱怨月经不规律、体重难以控制,或者脸上总是冒痘痘?这些看似普通的问题,可能隐藏着一种常见却容易被忽视的疾病——多囊卵巢综合征(PCOS)。P C O S是育龄期女性常见的内分泌代谢紊乱疾病,在国内的发病率约为5%...你是否经常听到身边的朋友抱怨月经不规律、体重难以控制,或者脸上总是冒痘痘?这些看似普通的问题,可能隐藏着一种常见却容易被忽视的疾病——多囊卵巢综合征(PCOS)。P C O S是育龄期女性常见的内分泌代谢紊乱疾病,在国内的发病率约为5%~10%。而在无排卵性不孕症患者中,这一比例甚至高达30%~60%。展开更多
目的:PCOS模型动物卵巢细胞内PI3K信号通路的变化与卵巢内分泌功能关系密切,本研究旨在探索PCOS模型大鼠卵巢颗粒细胞中PI3K信号通路的变化趋势,并探讨隐丹参酮调节PCOS模型大鼠颗粒细胞功能的可能机制。方法:将从空白对照大鼠及PCOS模...目的:PCOS模型动物卵巢细胞内PI3K信号通路的变化与卵巢内分泌功能关系密切,本研究旨在探索PCOS模型大鼠卵巢颗粒细胞中PI3K信号通路的变化趋势,并探讨隐丹参酮调节PCOS模型大鼠颗粒细胞功能的可能机制。方法:将从空白对照大鼠及PCOS模型大鼠获得的颗粒细胞分别分为空白组(空白对照组获取)、模型组(DHEA诱导PCOS模型大鼠获取)和隐丹参酮组(DHEA诱导PCOS模型大鼠获取,300 n M终浓度隐丹参酮处理24 h)进行体外培养及相应药物的预后。对各组细胞进行凋亡比率、培养液激素水平、甾体激素合成酶表达水平、葡萄糖摄取能力及PI3K信号通路蛋白表达水平检测。结果:隐丹参酮能够显著缓解PCOS模型大鼠颗粒细胞葡萄糖摄取能力及培养液激素水平的异常;在显著提高卵巢颗粒细胞PI3K信号通路关键分子磷酸化水平的同时,降低了PCOS模型大鼠颗粒细胞甾体激素合成酶STAR及CYP17的表达水平。结论:隐丹参酮治疗PCOS模型大鼠生殖机能异常的机制与PI3K途径的激活密切相关。展开更多
文摘Objective:To screen potential serum differential proteins in insulin resistance(IR)complicated with polycystic ovary syndrome(PCOS)using isobaric tags for relative and absolute quantitation(i TRAQ)combined with ultra-high performance liquid chromatography-tandem mass spectrometry(LC-MS/MS).Methods:A total of 20 patients diagnosed with PCOS in our hospital were selected,including 10 cases of simple PCOS and 10 cases of PCOS+IR.i TRAQ combined with LC-MS/MS was used for proteomic analysis to identify serum differential proteins.Bioinformatics analyses,including gene ontology(GO),Kyoto encyclopedia of genes and genomes(KEGG),and protein-protein interaction(PPI)analysis,were conducted to understand the biological processes,cellular components,and molecular functions of the differentially expressed proteins detected by the two methods.Results:A total of 454,675 secondary spectra were detected by iTRAQ,with 14,376 matched spectra.Combined with LC-MS/MS,74,386peptides,47,542 unique peptide sequences,and 54,675 proteins were identified.A total of 249 differentially expressed proteins with a fold change≥1.30 or≤0.83 were found,among which 9 had a P-value<0.05.There were 5 up-regulated and 4 down-regulated proteins,namely RPAP3,ALDH6A1,COX20,RASSF3,ALPK2,NANOS1,FAM210A,CHGA,and CGA.These differential proteins were imported into the STRING database for PPI network analysis,which revealed 21 nodes and 69 edges with a PPI enrichment P-value<0.001.KEGG enrichment results included hsa04913(Ovarian steroidogenesis),hsa04024(cAMP signaling pathway),and hsa04080(Neuroactive ligand-receptor interaction).Conclusion:The combination of i TRAQ and LC-MS/MS technologies identified nine differential proteins including CHGA and CGA,which are mainly enriched in pathways related to ovarian steroidogenesis,c AMP signaling,and neuroactive ligand-receptor interaction.This combination provides a powerful platform for exploring the pathogenesis of IR complicated with PCOS and discovering new biomarkers.
文摘你是否经常听到身边的朋友抱怨月经不规律、体重难以控制,或者脸上总是冒痘痘?这些看似普通的问题,可能隐藏着一种常见却容易被忽视的疾病——多囊卵巢综合征(PCOS)。P C O S是育龄期女性常见的内分泌代谢紊乱疾病,在国内的发病率约为5%~10%。而在无排卵性不孕症患者中,这一比例甚至高达30%~60%。
文摘目的:PCOS模型动物卵巢细胞内PI3K信号通路的变化与卵巢内分泌功能关系密切,本研究旨在探索PCOS模型大鼠卵巢颗粒细胞中PI3K信号通路的变化趋势,并探讨隐丹参酮调节PCOS模型大鼠颗粒细胞功能的可能机制。方法:将从空白对照大鼠及PCOS模型大鼠获得的颗粒细胞分别分为空白组(空白对照组获取)、模型组(DHEA诱导PCOS模型大鼠获取)和隐丹参酮组(DHEA诱导PCOS模型大鼠获取,300 n M终浓度隐丹参酮处理24 h)进行体外培养及相应药物的预后。对各组细胞进行凋亡比率、培养液激素水平、甾体激素合成酶表达水平、葡萄糖摄取能力及PI3K信号通路蛋白表达水平检测。结果:隐丹参酮能够显著缓解PCOS模型大鼠颗粒细胞葡萄糖摄取能力及培养液激素水平的异常;在显著提高卵巢颗粒细胞PI3K信号通路关键分子磷酸化水平的同时,降低了PCOS模型大鼠颗粒细胞甾体激素合成酶STAR及CYP17的表达水平。结论:隐丹参酮治疗PCOS模型大鼠生殖机能异常的机制与PI3K途径的激活密切相关。
