In transgenic process, a foreign gene can be integrated in the host genome in two directions, which may influence its expression. In order to study the effects of insertal orientation, the gfp reporter gene was insert...In transgenic process, a foreign gene can be integrated in the host genome in two directions, which may influence its expression. In order to study the effects of insertal orientation, the gfp reporter gene was inserted in the isiAB locus of Synechococcus sp. PCC7942 in different directions, and the GFP expression levels and the growth of the transgenic algae were compared. It was showed that the gfp gene could express in each direction, and no significant difference was detected on algal growth and GFP expression levels between the two recombinant algae.展开更多
蓝藻Synechococcussp.PCC7942 HCO3-高亲和转运蛋白操纵子基因cmpABCD是其CO2浓缩机制中的调控基因之一.本研究用携带潮霉素B磷酸转移酶基因(hygromyc in B pho transferase,hpt)筛选标记的同源双臂整合载体pUC-HATH转化蓝藻Synechococc...蓝藻Synechococcussp.PCC7942 HCO3-高亲和转运蛋白操纵子基因cmpABCD是其CO2浓缩机制中的调控基因之一.本研究用携带潮霉素B磷酸转移酶基因(hygromyc in B pho transferase,hpt)筛选标记的同源双臂整合载体pUC-HATH转化蓝藻Synechococcussp.PCC7942,以潮霉素B作为筛选试剂筛选出具潮霉素B抗性的转化藻,运用引物PCR方法证实潮霉素B磷酸转移酶基因表达盒通过质粒pUC-HATH的介导已定点插入蓝藻Synechococcussp.PCC7942基因组中,成功地构建了具有潮霉素B抗性的cmpBCD基因插入失活突变藻株.并最终通过比较野生藻Synechococcussp.PCC7942和突变藻Synechococcussp.PCC7942在不同Na2CO3浓度的改良BG-11培养基中生长特性,探讨了HCO3-高亲和转运蛋白操纵子cmpABCD基因失活对藻体生长的影响.展开更多
基金supported Science Foundation of China by the National Natural(No.30471317).
文摘In transgenic process, a foreign gene can be integrated in the host genome in two directions, which may influence its expression. In order to study the effects of insertal orientation, the gfp reporter gene was inserted in the isiAB locus of Synechococcus sp. PCC7942 in different directions, and the GFP expression levels and the growth of the transgenic algae were compared. It was showed that the gfp gene could express in each direction, and no significant difference was detected on algal growth and GFP expression levels between the two recombinant algae.
文摘蓝藻Synechococcussp.PCC7942 HCO3-高亲和转运蛋白操纵子基因cmpABCD是其CO2浓缩机制中的调控基因之一.本研究用携带潮霉素B磷酸转移酶基因(hygromyc in B pho transferase,hpt)筛选标记的同源双臂整合载体pUC-HATH转化蓝藻Synechococcussp.PCC7942,以潮霉素B作为筛选试剂筛选出具潮霉素B抗性的转化藻,运用引物PCR方法证实潮霉素B磷酸转移酶基因表达盒通过质粒pUC-HATH的介导已定点插入蓝藻Synechococcussp.PCC7942基因组中,成功地构建了具有潮霉素B抗性的cmpBCD基因插入失活突变藻株.并最终通过比较野生藻Synechococcussp.PCC7942和突变藻Synechococcussp.PCC7942在不同Na2CO3浓度的改良BG-11培养基中生长特性,探讨了HCO3-高亲和转运蛋白操纵子cmpABCD基因失活对藻体生长的影响.
