A human lung squamous carcinoma was transplanted and passaged in Swiss-DF nude mice, called LSX-83, for more than five years in our laboratory. The morphological characteristics of the original tumor were maintained i...A human lung squamous carcinoma was transplanted and passaged in Swiss-DF nude mice, called LSX-83, for more than five years in our laboratory. The morphological characteristics of the original tumor were maintained in passages from 4 to 33. But from the 35th generation, an increasing amount of tonofilaments and nuclear segregation with typical features was found with electron microscopy. The C-type virus particles were first detected in extra cellular space after 40 passages. The viruses were observed in different stages of growth, but their distribution and number did not show apparent change up to 54 passages. Such findings suggest that LSX-83 cells probably possess certain barrier of resistance against C-type viruses. The relation between C-type viruses and the morphological changes of LSX-83 cells was discussed.展开更多
Low pathogenic Avian Influenza (AI) virus has the ability to evolve to high pathogenic viruses resulting in significant economic losses in the poultry sector. This study aims at assessing the impact of H9N2 viral pass...Low pathogenic Avian Influenza (AI) virus has the ability to evolve to high pathogenic viruses resulting in significant economic losses in the poultry sector. This study aims at assessing the impact of H9N2 viral passaging in broilers and its relatedness to pathogenicity and amino acid (a.a) sequences of the hemagglutinin (HA) cleavage site and neuraminidase (NA) stalk. The original H9N2 AI virus (P0) was used to challenge ten-21 days old broilers. Individual recovery of H9N2 virus from homogenates of trachea, lungs and airsacs was attempted in 9 days old chicken embryos, as a conclusion of the first passage (P1). Tracheal isolates of H9N2 were passaged for a second (P2) and a third (P3) time in broilers, followed by a similar embryonic recovery procedure. The a.a. sequence of a part of HA1 cleavage site and Neuraminidase stalk were compared among the differently passaged viruses;an assessement of the relatedness of the determined a.a. sequences to the pathogenicity in broilers, based on frequency of mortality, morbidity signs, gross and microscopic lesions at 3 days post challenge with the P1, P2, and P3-H9N2, is concluded. An increase in certain morbidity signs and specific lesions was observed in P2- and P3-H9N2 challenged broilers compared to birds challenged with P1-H9N2. A conserved R-S-S-R amino acid sequence at the HA1 cleavage site was observed in the differently passaged H9N2, associated with a variability in the NA stalk-a.a sequences. The passaging of the low pathogenic H9N2 virus in broilers leads to a trend of increase in pathogenicity, manifested in higher frequency of morbidity signs, and of specific gross and microscopic lesions of the examined organs. This passaging was associated with a conserved a.a. sequence of the hemaglutinin cleavage site and a variability in the sequence of the neuraminidase stalk. A detailed study of the potential of the detected variability in the neuraminidase stalk of H9N2 in induction of a higher pathogenicity in broilers will be the subject of future investigations.展开更多
We have previously reported on both the osteogenic potential of hydroxyapatite (HA) combined with bone marrow-derived mesenchymal stem cells (BMSCs) and a method involving osteogenic matrix cell sheet transplantation ...We have previously reported on both the osteogenic potential of hydroxyapatite (HA) combined with bone marrow-derived mesenchymal stem cells (BMSCs) and a method involving osteogenic matrix cell sheet transplantation of BMSCs. In the present study, we assessed the osteogenic potential of serially-passaged BMSCs, both in vitro and in vivo. We also assessed whether an additional cell-loading technique can regain the osteogenic potential of the constructs combined with serially-passaged BMSCs. The present study revealed that passage (P) 1 cells cultured in osteogenic-induced medium showed strong positive staining for alkaline phosphatase (ALP) and Alizarin Red S, whereas P3 cells showed faint staining for ALP, with no Alizarin Red S staining. Staining of P1, P2 and P3 cells were progressively weaker, indicating that the osteogenic potential of the serially-passaged rat BMSCs is lost after P3 in vitro. The in vivo study showed that little bone formation was observed in the HA constructs seeded with P3 cells, 4 weeks after subcutaneous implantation. However, P3 cell/HA constructs which had increased cell-loading showed abundant bone formation within the pores of the HA construct. ALP and osteocalcin mRNA expression in these constructs was significantly higher than that of constructs with regular cell-seeding. The present study indicates that the osteogenic potential of the constructs with serially-passaged BMSCs is increased by additional cell-loading. This method can be applied to cases requiring hard tissue reconstruction, where BMSCs require serial expansion of cells.展开更多
Alice opened the door and found that it led into a small passage,not much larger than a rat⁃hole:she knelt down and looked along the passage into the loveliest garden you ever saw.How she longed to get out of that dar...Alice opened the door and found that it led into a small passage,not much larger than a rat⁃hole:she knelt down and looked along the passage into the loveliest garden you ever saw.How she longed to get out of that dark hall,and wander about among those beds of bright flowers and those cool fountains,but she could not even get her head through the doorway;“and even if my head would go through,”thought poor Alice,“it would be of very little use without my shoulders.Oh,how I wish I could shut up like a telescope!I think I could,if I only know how to begin.”For,you see,so many out⁃of⁃the⁃way things had happened lately,that Alice had begun to think that very few things indeed were really impossible.展开更多
1Legal requirements The author(s)guarantee(s)that the manuscript will not be published elsewhere in any language without the consent of the copyright holders,that the rights of third parties will not be violated,and t...1Legal requirements The author(s)guarantee(s)that the manuscript will not be published elsewhere in any language without the consent of the copyright holders,that the rights of third parties will not be violated,and that the publisher will not be held legally responsible should there be any claims for compensation.Authors wishing to include figures or text passages that have already been published elsewhere are required to obtain permission from the copyright holder(s)and to include evidence that such permission has been granted when submitting their papers.Any material received without such evidence will be assumed to originate from the authors.展开更多
1Legal requirements.The author(s)guarantee(s)that the manuscript will not be published elsewhere in any language without the consent of the copyright holders,that the rights of third parties will not be violated,and t...1Legal requirements.The author(s)guarantee(s)that the manuscript will not be published elsewhere in any language without the consent of the copyright holders,that the rights of third parties will not be violated,and that the publisher will not be held legally responsible should there be any claims for compensation.Authors wishing to include figures or text passages that have already been published elsewhere are required to obtain permission from the copyright holder(s)and to include evidence that such permission has been granted when submitting their papers.Any material received without such evidence will be assumed to originate from the authors.展开更多
NEW MATTER MATTERS.Oriental Outlook.17 April.The Shenzhen-Zhongshan Link,a major cross-sea passage in Guangdong Province,stands as a testament to modern engineering.To meet the demands of its harsh marine environment,...NEW MATTER MATTERS.Oriental Outlook.17 April.The Shenzhen-Zhongshan Link,a major cross-sea passage in Guangdong Province,stands as a testament to modern engineering.To meet the demands of its harsh marine environment,Chinese researchers have developed an advanced protective coating.Reinforced with ultrathin materials,this innovative solution is designed to extend the lifespan of steel structures well beyond a century by shielding them from corrosion.展开更多
Satellite communication technology has emerged as a key solution to address the challenges of data transmission in remote areas.By overcoming the limitations of traditional terrestrial communication networks,it enable...Satellite communication technology has emerged as a key solution to address the challenges of data transmission in remote areas.By overcoming the limitations of traditional terrestrial communication networks,it enables long-distance data transmission anytime and anywhere,ensuring the timely and accurate delivery of water level data,which is particularly crucial for fishway water level monitoring.To enhance the effectiveness of fishway water level monitoring,this study proposes a multi-task learning model,AS-SOMTF,designed for real-time and comprehensive prediction.The model integrates auxiliary sequences with primary input sequences to capture complex relationships and dependencies,thereby improving representational capacity.In addition,a novel timeseries embedding algorithm,AS-SOM,is introduced,which combines generative inference and pooling operations to optimize prediction efficiency for long sequences.This innovation not only ensures the timely transmission of water level data but also enhances the accuracy of real-time monitoring.Compared with traditional models such as Transformer and Long Short-Term Memory(LSTM)networks,the proposed model achieves improvements of 3.8%and 1.4%in prediction accuracy,respectively.These advancements provide more precise technical support for water level forecasting and resource management in the Diqing Tibetan Autonomous Prefecture of the Lancang River,contributing to ecosystem protection and improved operational safety.展开更多
Oral squamous cell carcinoma(OSCC)constitutes 90%of oral tumors.Advanced cases severely impair patients'life quality of life due to anatomical location and limited therapies.Conventional treatments often induce dr...Oral squamous cell carcinoma(OSCC)constitutes 90%of oral tumors.Advanced cases severely impair patients'life quality of life due to anatomical location and limited therapies.Conventional treatments often induce drug resistance or recurrence.Patientderived xenograft(PDX)models are widely used to simulate tumor progression and drug responses,serving as translational tools for precision medicine.This study aimed to establish drug-resistant OSCC PDX models.Human OSCC tissues were transplanted into immunodeficient mice and passaged(P1–P2).At P2(tumor volume:40–80 mm^(3)),mice received cisplatin(1 mg/kg,three times/week)with cetuximab(1 mg/kg,weekly),GSK690693(10 mg/kg,five times/week),or rapamycin(4 mg/kg,five times/week).PDX tissues from groups with less-therapeutic response(manifested as larger tumor volumes)were serially passaged to assess treatment efficacy.Tumor tissues with diminished drug sensitivity underwent histopathological analysis and identified stability of their tumor characteristics using hematoxylin–eosin(HE)and immunohistochemical staining after one additional passage and retreatment.Results demonstrated that successive passaging accelerates tumor growth.First-generation treatments showed universal sensitivity.At P2,cisplatin–cetuximab and rapamycin groups remained sensitive,whereas GSK690693 efficacy declined.Continued passaging of GSK690693-treated tumors confirmed resistance,as evidenced by exhibiting enhanced malignant characteristics at histological level.The GSK690693-resistant model was established first,whereas resistant models of other treatment groups were established according to similar protocols.These findings suggest that sequential passaging and drug exposure in PDX models recapitulated clinical tumor evolution,enabling the development of drug-resistant OSCC models.This study can offer methodological insights for precision therapy of OSCC.展开更多
We present a comprehensive extension of the integral of first passage times(IFS)method to investigate the adsorption kinetics of polymers with multiple binding sites on planar surfaces.While effective for single-point...We present a comprehensive extension of the integral of first passage times(IFS)method to investigate the adsorption kinetics of polymers with multiple binding sites on planar surfaces.While effective for single-point adsorption,the original IFS method was limited in capturing the complex kinetics of multi-point adsorption due to inadequate reaction coordinates and theoretical frameworks.Our approach introduces a center-of-mass-based reaction coordinate and a generalized kinetic model that accounts for multi-barrier free energy landscapes characteristic of collective polymer diffusion and binding.This theoretical advancement,implemented using the adaptive bias force method for efficient sampling,enables prediction of adsorption kinetics across timescales from nanoseconds to seconds.Our results demonstrate that adsorption behavior is governed by two key factors:the number of binding monomers primarily controls desorption barriers and long-term stability,while the configuration of pre-adsorbed layers significantly modulates both adsorption and desorption rates.Polymers with three or more binding sites exhibit effectively irreversible adsorption due to exponentially increasing desorption barriers,whereas different adsorbed layer configurations lead to distinct equilibrium coverages and kinetic profiles.This extended IFS framework provides critical insights for designing functional surfaces in nanoscale sensing,macromolecular recognition,and tailored polymeric coatings where precise control over adsorption kinetics is essential.展开更多
Global warming has made the regular operation of Arctic routes possible.This study selects hub ports based on infrastructure conditions and sea ice status,and then designs two pendulum route solutions for the Northeas...Global warming has made the regular operation of Arctic routes possible.This study selects hub ports based on infrastructure conditions and sea ice status,and then designs two pendulum route solutions for the Northeast Passage according to the distance between hub ports and ice-covered areas.We employ an evaluation framework combining annual profit metrics with discounted net present value(NPV)analysis,conducting probabilistic economic assessments through Monte Carlo simulations(20,000 iterations).Key findings indicate that(1)both solutions demonstrate>90%probability of economic viability and(2)Solution I′,with hub ports closer to ice-covered areas than those in Solution II,yields 5.02%higher mean annual profit and 4.69%greater NPV.The results indicate that pendulum routes in the Northeast Passage can achieve economic benefits by enabling year-round regular operations.Moreover,shorter shipping distances between hub ports and ice-covered areas enhance economic viability.展开更多
Global warming has led to a gradual extension of the navigable window for the Arctic Route,providing a realistic possibility for the normalized commercial operation of the Northeast Passage(NEP).Based on the changes i...Global warming has led to a gradual extension of the navigable window for the Arctic Route,providing a realistic possibility for the normalized commercial operation of the Northeast Passage(NEP).Based on the changes in the navigable window of the NEP,Russia’s proposed nuclear-powered icebreaker construction scheme,and China’s potential development of a moderately sized ice-class fleet,this study establishes three scenarios for the commercial operation of the NEP.These scenarios include:(a)normalized summer operational scenario(from July to October each year),(b)normalized summer-autumn operational scenario(from June to January of the following year),and(c)normalized year-round operational scenario(12 months per year).The cargo transportation potential of the NEP under three normalized operational scenarios was predicted based on the grey prediction model.On this basis,construction scale plans for China’s ice-class fleet to meet cargo transportation demands under the three normalized operational scenarios were designed.The economic benefits of different plans were evaluated using a profit-maximization linear programming model.The research results show the following:(1)The cargo transportation potential of the NEP demonstrates a rapid growth trend in the future,with annual throughput under year-round normalized operations expected to exceed 100 million tonnes and reach 297 million tonnes.(2)Under different normalized operational scenarios,the fleet scale and vessel type composition vary.Under the normalized summer operational scenario,the optimal scale for China’s ice-class fleet is 20 vessels,consisting solely of ships classed as PC7 by the International Association of Classification Societies(IACS).Under the normalized summer-autumn operational scenario,the optimal fleet scale is 31 vessels,including 30 IACS PC7 ships and 1 IACS PC3 ship.Under the normalized year-round operational scenario,the optimal fleet scale is 45 vessels,composed of 30 IACS PC7 ships,8 IACS PC3 ships,and 7 IACS PC2 ships.(3)Among the three normalized operational scenarios,the normalized year-round operational scenario yields the best economic benefits for the fleet scale,while the normalized summer operational scenario yields the lowest economic benefits.展开更多
Tricellulin,a key tricellular tight junction(TJ)protein,is essential for maintaining the barrier integrity of acinar epithelia against macromolecular passage in salivary glands.This study aims to explore the role and ...Tricellulin,a key tricellular tight junction(TJ)protein,is essential for maintaining the barrier integrity of acinar epithelia against macromolecular passage in salivary glands.This study aims to explore the role and regulatory mechanism of tricellulin in the development of salivary gland hypofunction in Sjögren’s syndrome(SS).Employing a multifaceted approach involving patient biopsies,non-obese diabetic(NOD)mice as a SS model,salivary gland acinar cell-specific tricellulin conditional knockout(TricCKO)mice,and IFN-γ-stimulated salivary gland epithelial cells,we investigated the role of tricellulin in SS-related hyposalivation.Our data revealed diminished levels of tricellulin in salivary glands of SS patients.Similarly,NOD mice displayed a reduction in tricellulin expression from the onset of the disease,concomitant with hyposecretion and an increase in salivary albumin content.Consistent with these findings,TricCKO mice exhibited both hyposecretion and leakage of macromolecular tracers when compared to control animals.Mechanistically,the JAK/STAT1/miR-145 axis was identified as mediating the IFN-γ-induced downregulation of tricellulin.Treatment with AT1001,a TJ sealer,ameliorated epithelial barrier dysfunction,restored tricellulin expression,and consequently alleviated hyposalivation in NOD mice.Importantly,treatment with miR-145 antagomir to specifically recover the expression of tricellulin in NOD mice significantly alleviated hyposalivation and macromolecular leakage.Collectively,we identified that tricellulin deficiency in salivary glands contributed to hyposalivation in SS.Our findings highlight tricellulin as a potential therapeutic target for hyposecretion,particularly in the context of reinforcing epithelial barrier function through preventing leakage of macromolecules in salivary glands.展开更多
The author attempts to interpret the theme of A Passsage to India by analyzing the major events and characterization. It is indicated in the thesis that E.M.Forster highlights the failure of aspired understanding ...The author attempts to interpret the theme of A Passsage to India by analyzing the major events and characterization. It is indicated in the thesis that E.M.Forster highlights the failure of aspired understanding between different races, different people and even within a person. In other words, in many cases people have aspirations for understanding, but in reality the real understanding is very difficult to fulfill.展开更多
IV.阅读理解。认真阅读下面两篇短文,每篇短文后各有五个问题,根据短文的内容从A、B、c、D四个选项中,选择一个正确答案。(本大题共10小题,每小题1分,共10分)Read the two passages and answer the questions. (10 points)Passage oneTh...IV.阅读理解。认真阅读下面两篇短文,每篇短文后各有五个问题,根据短文的内容从A、B、c、D四个选项中,选择一个正确答案。(本大题共10小题,每小题1分,共10分)Read the two passages and answer the questions. (10 points)Passage oneThe advantages and disadvantages of a large population has long been a subject of展开更多
Ⅳ.阅读理解。认真阅读下面两篇短文,每篇短文后各有五个问题,根据短文的内容从A、 B、C、D四个选项中,选择一个正确答案。 (本大题共10小题,每小题1分,共1O分)Read the two passages and answer the questions.(10 points) Passage One...Ⅳ.阅读理解。认真阅读下面两篇短文,每篇短文后各有五个问题,根据短文的内容从A、 B、C、D四个选项中,选择一个正确答案。 (本大题共10小题,每小题1分,共1O分)Read the two passages and answer the questions.(10 points) Passage One Every artist knows in his heart that he is展开更多
This thesis tries to study two novels:firstly,the performance of Orientalism in the novel A Passage to India;secondly the Exorcization of Orientalism in the novel the Woman Warrior written by Maxine Hong Kingston.
Ⅲ.Reading Comprehension 20%Questions 1 -4 are based on the following passage.Are you a team person? Are you at your best as part of a small tightly united group of dedicated workers? If so, the future may hold more f...Ⅲ.Reading Comprehension 20%Questions 1 -4 are based on the following passage.Are you a team person? Are you at your best as part of a small tightly united group of dedicated workers? If so, the future may hold more for you than you think.展开更多
There are many expressions using the word"bridge".So today,let’s talk about the stories of"bridge".英语中有很多表达中使用了"桥"一词,所以今天我们就来说说"桥"的故事吧。A bridge i...There are many expressions using the word"bridge".So today,let’s talk about the stories of"bridge".英语中有很多表达中使用了"桥"一词,所以今天我们就来说说"桥"的故事吧。A bridge is a structure that provides passage over something—such as a river,train tracks,a highway or a deep,wide opening in the ground.The word"bridge"can also展开更多
文摘A human lung squamous carcinoma was transplanted and passaged in Swiss-DF nude mice, called LSX-83, for more than five years in our laboratory. The morphological characteristics of the original tumor were maintained in passages from 4 to 33. But from the 35th generation, an increasing amount of tonofilaments and nuclear segregation with typical features was found with electron microscopy. The C-type virus particles were first detected in extra cellular space after 40 passages. The viruses were observed in different stages of growth, but their distribution and number did not show apparent change up to 54 passages. Such findings suggest that LSX-83 cells probably possess certain barrier of resistance against C-type viruses. The relation between C-type viruses and the morphological changes of LSX-83 cells was discussed.
文摘Low pathogenic Avian Influenza (AI) virus has the ability to evolve to high pathogenic viruses resulting in significant economic losses in the poultry sector. This study aims at assessing the impact of H9N2 viral passaging in broilers and its relatedness to pathogenicity and amino acid (a.a) sequences of the hemagglutinin (HA) cleavage site and neuraminidase (NA) stalk. The original H9N2 AI virus (P0) was used to challenge ten-21 days old broilers. Individual recovery of H9N2 virus from homogenates of trachea, lungs and airsacs was attempted in 9 days old chicken embryos, as a conclusion of the first passage (P1). Tracheal isolates of H9N2 were passaged for a second (P2) and a third (P3) time in broilers, followed by a similar embryonic recovery procedure. The a.a. sequence of a part of HA1 cleavage site and Neuraminidase stalk were compared among the differently passaged viruses;an assessement of the relatedness of the determined a.a. sequences to the pathogenicity in broilers, based on frequency of mortality, morbidity signs, gross and microscopic lesions at 3 days post challenge with the P1, P2, and P3-H9N2, is concluded. An increase in certain morbidity signs and specific lesions was observed in P2- and P3-H9N2 challenged broilers compared to birds challenged with P1-H9N2. A conserved R-S-S-R amino acid sequence at the HA1 cleavage site was observed in the differently passaged H9N2, associated with a variability in the NA stalk-a.a sequences. The passaging of the low pathogenic H9N2 virus in broilers leads to a trend of increase in pathogenicity, manifested in higher frequency of morbidity signs, and of specific gross and microscopic lesions of the examined organs. This passaging was associated with a conserved a.a. sequence of the hemaglutinin cleavage site and a variability in the sequence of the neuraminidase stalk. A detailed study of the potential of the detected variability in the neuraminidase stalk of H9N2 in induction of a higher pathogenicity in broilers will be the subject of future investigations.
文摘We have previously reported on both the osteogenic potential of hydroxyapatite (HA) combined with bone marrow-derived mesenchymal stem cells (BMSCs) and a method involving osteogenic matrix cell sheet transplantation of BMSCs. In the present study, we assessed the osteogenic potential of serially-passaged BMSCs, both in vitro and in vivo. We also assessed whether an additional cell-loading technique can regain the osteogenic potential of the constructs combined with serially-passaged BMSCs. The present study revealed that passage (P) 1 cells cultured in osteogenic-induced medium showed strong positive staining for alkaline phosphatase (ALP) and Alizarin Red S, whereas P3 cells showed faint staining for ALP, with no Alizarin Red S staining. Staining of P1, P2 and P3 cells were progressively weaker, indicating that the osteogenic potential of the serially-passaged rat BMSCs is lost after P3 in vitro. The in vivo study showed that little bone formation was observed in the HA constructs seeded with P3 cells, 4 weeks after subcutaneous implantation. However, P3 cell/HA constructs which had increased cell-loading showed abundant bone formation within the pores of the HA construct. ALP and osteocalcin mRNA expression in these constructs was significantly higher than that of constructs with regular cell-seeding. The present study indicates that the osteogenic potential of the constructs with serially-passaged BMSCs is increased by additional cell-loading. This method can be applied to cases requiring hard tissue reconstruction, where BMSCs require serial expansion of cells.
文摘Alice opened the door and found that it led into a small passage,not much larger than a rat⁃hole:she knelt down and looked along the passage into the loveliest garden you ever saw.How she longed to get out of that dark hall,and wander about among those beds of bright flowers and those cool fountains,but she could not even get her head through the doorway;“and even if my head would go through,”thought poor Alice,“it would be of very little use without my shoulders.Oh,how I wish I could shut up like a telescope!I think I could,if I only know how to begin.”For,you see,so many out⁃of⁃the⁃way things had happened lately,that Alice had begun to think that very few things indeed were really impossible.
文摘1Legal requirements The author(s)guarantee(s)that the manuscript will not be published elsewhere in any language without the consent of the copyright holders,that the rights of third parties will not be violated,and that the publisher will not be held legally responsible should there be any claims for compensation.Authors wishing to include figures or text passages that have already been published elsewhere are required to obtain permission from the copyright holder(s)and to include evidence that such permission has been granted when submitting their papers.Any material received without such evidence will be assumed to originate from the authors.
文摘1Legal requirements.The author(s)guarantee(s)that the manuscript will not be published elsewhere in any language without the consent of the copyright holders,that the rights of third parties will not be violated,and that the publisher will not be held legally responsible should there be any claims for compensation.Authors wishing to include figures or text passages that have already been published elsewhere are required to obtain permission from the copyright holder(s)and to include evidence that such permission has been granted when submitting their papers.Any material received without such evidence will be assumed to originate from the authors.
文摘NEW MATTER MATTERS.Oriental Outlook.17 April.The Shenzhen-Zhongshan Link,a major cross-sea passage in Guangdong Province,stands as a testament to modern engineering.To meet the demands of its harsh marine environment,Chinese researchers have developed an advanced protective coating.Reinforced with ultrathin materials,this innovative solution is designed to extend the lifespan of steel structures well beyond a century by shielding them from corrosion.
基金supported in part by the National Natural Science Foundation of China under Grant 62371181in part by the Changzhou Science and Technology International Cooperation Program under Grant CZ20230029The Institute of Information&Communications Technology Planning&Evaluation(IITP)grant funded by the Korea government(MSIT)(No.RS-2024-00396797,Development of core technology for intelligent O-RAN security platform).
文摘Satellite communication technology has emerged as a key solution to address the challenges of data transmission in remote areas.By overcoming the limitations of traditional terrestrial communication networks,it enables long-distance data transmission anytime and anywhere,ensuring the timely and accurate delivery of water level data,which is particularly crucial for fishway water level monitoring.To enhance the effectiveness of fishway water level monitoring,this study proposes a multi-task learning model,AS-SOMTF,designed for real-time and comprehensive prediction.The model integrates auxiliary sequences with primary input sequences to capture complex relationships and dependencies,thereby improving representational capacity.In addition,a novel timeseries embedding algorithm,AS-SOM,is introduced,which combines generative inference and pooling operations to optimize prediction efficiency for long sequences.This innovation not only ensures the timely transmission of water level data but also enhances the accuracy of real-time monitoring.Compared with traditional models such as Transformer and Long Short-Term Memory(LSTM)networks,the proposed model achieves improvements of 3.8%and 1.4%in prediction accuracy,respectively.These advancements provide more precise technical support for water level forecasting and resource management in the Diqing Tibetan Autonomous Prefecture of the Lancang River,contributing to ecosystem protection and improved operational safety.
基金National Natural Science Foundation of China,Grant/Award Number:82173399Young Elite Scientists Sponsorship Program by CAST,Grant/Award Number:2022QNRC001+2 种基金Beijing Natural Science Foundation,Grant/Award Number:7252096Beijing Natural Science Foundation-Haidian Original Innovation Joint Fund Project,Grant/Award Number:L222145CAMS&Comparative Medicine Center,PUMC (IACUC approval number:QC24002)
文摘Oral squamous cell carcinoma(OSCC)constitutes 90%of oral tumors.Advanced cases severely impair patients'life quality of life due to anatomical location and limited therapies.Conventional treatments often induce drug resistance or recurrence.Patientderived xenograft(PDX)models are widely used to simulate tumor progression and drug responses,serving as translational tools for precision medicine.This study aimed to establish drug-resistant OSCC PDX models.Human OSCC tissues were transplanted into immunodeficient mice and passaged(P1–P2).At P2(tumor volume:40–80 mm^(3)),mice received cisplatin(1 mg/kg,three times/week)with cetuximab(1 mg/kg,weekly),GSK690693(10 mg/kg,five times/week),or rapamycin(4 mg/kg,five times/week).PDX tissues from groups with less-therapeutic response(manifested as larger tumor volumes)were serially passaged to assess treatment efficacy.Tumor tissues with diminished drug sensitivity underwent histopathological analysis and identified stability of their tumor characteristics using hematoxylin–eosin(HE)and immunohistochemical staining after one additional passage and retreatment.Results demonstrated that successive passaging accelerates tumor growth.First-generation treatments showed universal sensitivity.At P2,cisplatin–cetuximab and rapamycin groups remained sensitive,whereas GSK690693 efficacy declined.Continued passaging of GSK690693-treated tumors confirmed resistance,as evidenced by exhibiting enhanced malignant characteristics at histological level.The GSK690693-resistant model was established first,whereas resistant models of other treatment groups were established according to similar protocols.These findings suggest that sequential passaging and drug exposure in PDX models recapitulated clinical tumor evolution,enabling the development of drug-resistant OSCC models.This study can offer methodological insights for precision therapy of OSCC.
基金financially supported by the National Natural Science Foundation of China(No.12374207)the Natural Science Foundation of Jiangsu Province(No.BK20233001)supported by the Big Data Computing Center of Southeast University。
文摘We present a comprehensive extension of the integral of first passage times(IFS)method to investigate the adsorption kinetics of polymers with multiple binding sites on planar surfaces.While effective for single-point adsorption,the original IFS method was limited in capturing the complex kinetics of multi-point adsorption due to inadequate reaction coordinates and theoretical frameworks.Our approach introduces a center-of-mass-based reaction coordinate and a generalized kinetic model that accounts for multi-barrier free energy landscapes characteristic of collective polymer diffusion and binding.This theoretical advancement,implemented using the adaptive bias force method for efficient sampling,enables prediction of adsorption kinetics across timescales from nanoseconds to seconds.Our results demonstrate that adsorption behavior is governed by two key factors:the number of binding monomers primarily controls desorption barriers and long-term stability,while the configuration of pre-adsorbed layers significantly modulates both adsorption and desorption rates.Polymers with three or more binding sites exhibit effectively irreversible adsorption due to exponentially increasing desorption barriers,whereas different adsorbed layer configurations lead to distinct equilibrium coverages and kinetic profiles.This extended IFS framework provides critical insights for designing functional surfaces in nanoscale sensing,macromolecular recognition,and tailored polymeric coatings where precise control over adsorption kinetics is essential.
文摘Global warming has made the regular operation of Arctic routes possible.This study selects hub ports based on infrastructure conditions and sea ice status,and then designs two pendulum route solutions for the Northeast Passage according to the distance between hub ports and ice-covered areas.We employ an evaluation framework combining annual profit metrics with discounted net present value(NPV)analysis,conducting probabilistic economic assessments through Monte Carlo simulations(20,000 iterations).Key findings indicate that(1)both solutions demonstrate>90%probability of economic viability and(2)Solution I′,with hub ports closer to ice-covered areas than those in Solution II,yields 5.02%higher mean annual profit and 4.69%greater NPV.The results indicate that pendulum routes in the Northeast Passage can achieve economic benefits by enabling year-round regular operations.Moreover,shorter shipping distances between hub ports and ice-covered areas enhance economic viability.
基金Funding by Social Science Research of Ministry of Education of the People’s Republic of China“Study on issues related on the development and utilization of the Arctic Passage”(Grant no.20JHQ016)is acknowledged.
文摘Global warming has led to a gradual extension of the navigable window for the Arctic Route,providing a realistic possibility for the normalized commercial operation of the Northeast Passage(NEP).Based on the changes in the navigable window of the NEP,Russia’s proposed nuclear-powered icebreaker construction scheme,and China’s potential development of a moderately sized ice-class fleet,this study establishes three scenarios for the commercial operation of the NEP.These scenarios include:(a)normalized summer operational scenario(from July to October each year),(b)normalized summer-autumn operational scenario(from June to January of the following year),and(c)normalized year-round operational scenario(12 months per year).The cargo transportation potential of the NEP under three normalized operational scenarios was predicted based on the grey prediction model.On this basis,construction scale plans for China’s ice-class fleet to meet cargo transportation demands under the three normalized operational scenarios were designed.The economic benefits of different plans were evaluated using a profit-maximization linear programming model.The research results show the following:(1)The cargo transportation potential of the NEP demonstrates a rapid growth trend in the future,with annual throughput under year-round normalized operations expected to exceed 100 million tonnes and reach 297 million tonnes.(2)Under different normalized operational scenarios,the fleet scale and vessel type composition vary.Under the normalized summer operational scenario,the optimal scale for China’s ice-class fleet is 20 vessels,consisting solely of ships classed as PC7 by the International Association of Classification Societies(IACS).Under the normalized summer-autumn operational scenario,the optimal fleet scale is 31 vessels,including 30 IACS PC7 ships and 1 IACS PC3 ship.Under the normalized year-round operational scenario,the optimal fleet scale is 45 vessels,composed of 30 IACS PC7 ships,8 IACS PC3 ships,and 7 IACS PC2 ships.(3)Among the three normalized operational scenarios,the normalized year-round operational scenario yields the best economic benefits for the fleet scale,while the normalized summer operational scenario yields the lowest economic benefits.
基金supported by the National Natural Science Foundation of China(grants 31972908,81991500,81991502,and 32030010)Beijing Natural Science Foundation(grant 7202082).
文摘Tricellulin,a key tricellular tight junction(TJ)protein,is essential for maintaining the barrier integrity of acinar epithelia against macromolecular passage in salivary glands.This study aims to explore the role and regulatory mechanism of tricellulin in the development of salivary gland hypofunction in Sjögren’s syndrome(SS).Employing a multifaceted approach involving patient biopsies,non-obese diabetic(NOD)mice as a SS model,salivary gland acinar cell-specific tricellulin conditional knockout(TricCKO)mice,and IFN-γ-stimulated salivary gland epithelial cells,we investigated the role of tricellulin in SS-related hyposalivation.Our data revealed diminished levels of tricellulin in salivary glands of SS patients.Similarly,NOD mice displayed a reduction in tricellulin expression from the onset of the disease,concomitant with hyposecretion and an increase in salivary albumin content.Consistent with these findings,TricCKO mice exhibited both hyposecretion and leakage of macromolecular tracers when compared to control animals.Mechanistically,the JAK/STAT1/miR-145 axis was identified as mediating the IFN-γ-induced downregulation of tricellulin.Treatment with AT1001,a TJ sealer,ameliorated epithelial barrier dysfunction,restored tricellulin expression,and consequently alleviated hyposalivation in NOD mice.Importantly,treatment with miR-145 antagomir to specifically recover the expression of tricellulin in NOD mice significantly alleviated hyposalivation and macromolecular leakage.Collectively,we identified that tricellulin deficiency in salivary glands contributed to hyposalivation in SS.Our findings highlight tricellulin as a potential therapeutic target for hyposecretion,particularly in the context of reinforcing epithelial barrier function through preventing leakage of macromolecules in salivary glands.
文摘The author attempts to interpret the theme of A Passsage to India by analyzing the major events and characterization. It is indicated in the thesis that E.M.Forster highlights the failure of aspired understanding between different races, different people and even within a person. In other words, in many cases people have aspirations for understanding, but in reality the real understanding is very difficult to fulfill.
文摘IV.阅读理解。认真阅读下面两篇短文,每篇短文后各有五个问题,根据短文的内容从A、B、c、D四个选项中,选择一个正确答案。(本大题共10小题,每小题1分,共10分)Read the two passages and answer the questions. (10 points)Passage oneThe advantages and disadvantages of a large population has long been a subject of
文摘Ⅳ.阅读理解。认真阅读下面两篇短文,每篇短文后各有五个问题,根据短文的内容从A、 B、C、D四个选项中,选择一个正确答案。 (本大题共10小题,每小题1分,共1O分)Read the two passages and answer the questions.(10 points) Passage One Every artist knows in his heart that he is
文摘This thesis tries to study two novels:firstly,the performance of Orientalism in the novel A Passage to India;secondly the Exorcization of Orientalism in the novel the Woman Warrior written by Maxine Hong Kingston.
文摘Ⅲ.Reading Comprehension 20%Questions 1 -4 are based on the following passage.Are you a team person? Are you at your best as part of a small tightly united group of dedicated workers? If so, the future may hold more for you than you think.
文摘There are many expressions using the word"bridge".So today,let’s talk about the stories of"bridge".英语中有很多表达中使用了"桥"一词,所以今天我们就来说说"桥"的故事吧。A bridge is a structure that provides passage over something—such as a river,train tracks,a highway or a deep,wide opening in the ground.The word"bridge"can also
