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银胶菊(Parthenium hysterophorus)中抗TMV活性成分的分离及活性测定 被引量:8
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作者 陈启建 欧阳明安 +1 位作者 谢联辉 林奇英 《激光生物学报》 CAS CSCD 2008年第4期544-548,共5页
采用活性跟踪法从银胶菊中分离获得抗病毒活性物质,经质谱和核磁共振分析,确定该物质为小白菊内酯。采用半叶枯斑法和叶圆盘法测定了其对烟草花叶病毒(Tobacco mosaic virus,TMV)的抑制效果,结果表明:0.2 mg/mL的小白菊内酯在混合接种... 采用活性跟踪法从银胶菊中分离获得抗病毒活性物质,经质谱和核磁共振分析,确定该物质为小白菊内酯。采用半叶枯斑法和叶圆盘法测定了其对烟草花叶病毒(Tobacco mosaic virus,TMV)的抑制效果,结果表明:0.2 mg/mL的小白菊内酯在混合接种和药液浸泡两种处理中均表现出较好的体外抑制TMV活性,其抑制率分别为69.0%和72.1%;在离体条件下对烟草叶圆盘中的烟草花叶病毒复制也具有良好的抑制效果,抑制率为72.3%。 展开更多
关键词 银胶菊 小白菊内酯 抗TMV活性
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Predicting suitable habitat of an invasive weed Parthenium hysterophorus under future climate scenarios in Chitwan Annapurna Landscape,Nepal 被引量:2
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作者 MAHARJAN Seerjana SHRESTHA Bharat Babu +4 位作者 JOSHI Mohan Dev DEVKOTA Anjana MUNIAPPAN Rangaswamy ADIGA Abhijin JHA Pramod Kumar 《Journal of Mountain Science》 SCIE CSCD 2019年第10期2243-2256,共14页
Chitwan-Annapuma Landscape(CHAL)in central Nepal is known for its rich biodiversity and the landscape is expected to provide corridors for species range shift in response to climate change.Environmental assessments ha... Chitwan-Annapuma Landscape(CHAL)in central Nepal is known for its rich biodiversity and the landscape is expected to provide corridors for species range shift in response to climate change.Environmental assessments have identified biological invasions and other anthropogenic activities as major threats to the biodiversity in the CHAL.One of the rapidly spreading Invasive Alien Plant species(IAPs)in the CHAL is Parthenium hysterophorus L.,a neotropical invasive weed of global significance.This study aimed to investigate the current and future projected suitable habitat of P.hysterophorus in the CHAL using MaxEnt modelling in three'Representative Concentration Pathways'(RCPs 2.6,4.5 and 8.5)corresponding to different greenhouse gases emissiontrajectories for the year 2050 and 2070.A total of 288species occurrence points,six bioclimatic variablesmean diurnal range,isothermality,annual precipitation,precipitation of driest month,precipitation seasonality,precipitation of driest quarter and two topographic variables(aspect and slope)were selected for MaxEnt modelling.Potential range shift in terms of increase or decline in the suitable habitat areas under the projected scenarios were calculated.Slope and annual precipitation were the most important variables that explained the current distribution of P.hysterophorus.Twenty percent of the total area of CHAL was predicted to be suitable habitat for the growth of P.hysterophorus in the current climatic condition.Highest gain in the suitable habitat of this noxious weed was found under RCP 4.5 scenario in 2050 and 2070.whereas there will be a loss in thesuitable habitat under RCP 8.5 scenario in 2050 and2070.Out of four physiographic regions present in CHAL,three regions-Siwalik,Middle Mountain and High Mountain have suitable habitat for P.hysterophorus under current climatic condition.The mountainous region is likely to be affected more than the Siwalik region by further spread of P.fhysteropfhorus in the future under low(RCP 2.6)to medium(RCP 4.5)emission scenarios.The suitable habitat for this weed is likely to increase in the protected areas of mountain regions(Langtang National Park,Annapurna Conservation Area and Manaslu Conservation Area)in the future.The results have revealed a risk of spreading P.hysterophorus from present localities to non-invaded areas in the current and future climatic condition.Such risk needs to be considered by decision makers and resource managers while planning for effective management of this weed to reduce its ecological and economic impacts in the CHAL. 展开更多
关键词 parthenium WEED Ecological NICHE Model MAXENT Invasive species HABITAT SUITABILITY
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Phenolic Acids from <i>Parthenium hysterophorus</i>: Evaluation of Bioconversion Potential as Free Radical Scavengers and Anticancer Agents 被引量:1
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作者 Richa Panwar Asvene Kumar Sharma +1 位作者 Dharm Dutt Vikas Pruthi 《Advances in Bioscience and Biotechnology》 2015年第1期11-17,共7页
Parthenium hysterophorus is a globally recognized invasive alien weed that prominently colonizes grazing areas and cultivated lands causing adverse effect on crop production. Major allelochemicals released from parthe... Parthenium hysterophorus is a globally recognized invasive alien weed that prominently colonizes grazing areas and cultivated lands causing adverse effect on crop production. Major allelochemicals released from parthenium include sesqueterpene lactones and phenolic acids. Among these the presence of caffeic, vanillic and ferulic acids is of industrial significance as they possess potent free radical scavenging and anticancer activities. This study reports for the first time, high total phenolic acid content (20.82 ± 0.82 mg GAE/g dry sample) in parthenium. The GC-MS analysis indicated the presence of ferulic, p-coumaric, vanillic and gallic acid as major phenolic components. Free radical scavenging activity of the phenolic acids extract gave an EC50 value 130.4 μg/ml when measured using DPPH assay. Anticancer activity of parthenium phenolic extract against A-498 (IC50 0.5237 μg/ml) and MDA-MB231 (IC50 and 0.2685 μg/ml) cancerous cell lines indicated its potential to be used as anticancer agent. 展开更多
关键词 parthenium hysterophorus PHENOLIC ACIDS GC-MS ANTIOXIDANT Bioconversion
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Is intraspecific trait differentiation in Parthenium hysterophorus a consequence of hereditary factors and/or phenotypic plasticity?
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作者 Amarpreet Kaur Shalinder Kaur +1 位作者 Harminder Pal Singh Daizy R.Batish 《Plant Diversity》 SCIE CAS CSCD 2023年第5期611-620,共10页
Of the various strategies adopted by an invasive plant species for expanding its niche breadth,phenotypic differentiation(either due to plasticity and/or adaptive evolution) is proven to be the most successful.Lately,... Of the various strategies adopted by an invasive plant species for expanding its niche breadth,phenotypic differentiation(either due to plasticity and/or adaptive evolution) is proven to be the most successful.Lately,we studied the persistence of substantial morpho-functional variations within the individuals of alien invasive plant,Parthenium hysterophorus in Chandigarh,India,through field surveys.Based on observed differences,the individuals were categorized into two morphotypes,PAand PB.PAhad higher leaf area,leaf biomass,and chlorophyll content as compared with PB.However,PBhad a higher stem circumference,stem specific density,twig dry matter content,profuse branching,bigger canopy,and better reproductive output than PA.To substantiate the persistence of intraspecific variations in P. hysterophorus and to deduce the possible genesis of these variations,we propagated both the morphotypes under experimental conditions in winter and summer.Apart from the key morpho-functional differences observed during the field studies,protein and carbohydrate metabolism were studied in leaves and roots of the propagated plants.Differences in plant metabolism were observed only during the early growth period,whereas the morpho-functional traits varied in the mature flowering plants.The effect of growth season was highly significant on all the studied morpho-functional and biochemical parameters(p ≤0.05).Parent morphotypes(P) and interactions between morphotypes and seasons significantly affected several growth parameters(p ≤0.05).The analyses revealed that the contrasting growth conditions at the time of transplantation and early growth may regulate the phenotype of P. hysterophorus.The pattern of intraspecific variations observed during the study is justified to consider morphotype PAas winter biotype and morphotype PBas summer biotype of P. hysterophorus.The study points towards the role of plasticity or a combination of genetic and environmental(G×E) factors in producing the phenotypic variability observed in the population of P. hysterophorus. 展开更多
关键词 Biochemical traits Carbohydrate metabolism Morpho-functional traits Phenotypic variations Protein metabolism Ragweed parthenium
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Distribution pattern and ecological determinants of an invasive plant Parthenium hysterophorus L., in Malakand division of Pakistan
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作者 Nasrullah KHAN Khudija BIBI Rafi ULLAH 《Journal of Mountain Science》 SCIE CSCD 2020年第7期1670-1683,共14页
After habitat loss,drastic effects of biological invasion caused by alien invasive species to the native biodiversity have been acknowledged worldwide by the agriculturists,ecologists,and governments as it is more ove... After habitat loss,drastic effects of biological invasion caused by alien invasive species to the native biodiversity have been acknowledged worldwide by the agriculturists,ecologists,and governments as it is more overwhelming than pollution,harvest and disease combined.One such example of biological invasion is P.hysterophorus which is considered to be one of the topmost noxious weeds threatening ecosystems diversity in the world,particularly in Pakistan due to its invasive success and generalist nature.Yet no studies have explored the pattern and determinants of plant biodiversity in the Parthenium dominated landscapes.Here we explored the current distribution pattern of this species with relation to environmental variables in the Malakand division of northern Pakistan.Twenty-three sites dominated by P.hysterophorus were identified through a participatory mapping process combined with traditional ecological knowledge and quantitative inventories were made.In total,we found 62 plant species in 55 genera and 32 families,predominately from Asteraceae,Poaceae,and Solanaceae.Ward’s agglomerative clustering procedure showed three well-differentiated,and ecologically meaningful but overlapping vegetation communities.P.hysterophorus was found to be the leading species with an average Importance value ranging from 33.28%to 65.59%.Based on our criteria,surprisingly we found significantly less to be fully(30%)or partially invaded(21%)than the less invaded(47%)plots,indicating the gradual invasive success of the species across the landscape.Canonical Correspondence Analysis(CCA-ordination)has explained 36.9%of the cumulative variance by the first three axes and identified elevation(r=-0.526),slope(r=0.438)and percent sand(r=-0.474)to be the major environmental factors affecting the distribution of P.hysterophorus and associated vegetation.These results suggested that elevation and percent sand negatively affect Parthenium distribution whereas slope has a positive relationship thereby promoting its populations in the region.The generalist nature of this invasive species is likely to have an overall adverse negative effect on the ecosystem functioning and cannot be ignored.Therefore,quick actions should be taken for the management of this noxious weed to avoid economic consequences and potential threats to fragile ecosystems such as that in Malakand division. 展开更多
关键词 Habitat lost Biological invasion parthenium hysterophorus Multivariate analysis Conservation and Management
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Comparative Efficacy of Weed Control Practices for Parthenium Weed and Sunflower Crop under Varying Tillage Systems
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作者 Noor Ahmad Rana Nadeem Abbas +1 位作者 Asif Tanveer Zulfiqar Ahmad Saqib 《Phyton-International Journal of Experimental Botany》 SCIE 2020年第3期727-742,共16页
Parthenium poses serious threat to modern crop production system and necessitate evaluating control practices for its effective management.Efficacy of different weed control practices for controlling parthenium was ex... Parthenium poses serious threat to modern crop production system and necessitate evaluating control practices for its effective management.Efficacy of different weed control practices for controlling parthenium was explored in conventional and deep tillage systems in the field conditions.Hand hoeing(20 and 35 days after emergence),S-Metolachlor(pre-emergence herbicide),sorghum straw mulch@5 tons ha-1 and combination of hand hoeing and sorghum straw mulch(hand hoeing at 20 and straw mulch at 35 days after emergence)were used as weed control practice.Weedy check where no weed control measure was applied was also included in this experiment for comparison.Results concluded that the all weed management treatments significantly reduced parthenium density,its fresh and dry biomass during both the years of study as compared to weedy check.Maximum sunflower achene yield was recorded in hand hoeing(20 and 35 days after emergence)in combination with deep tillage.So,mold bold plough used for the purpose of deep tillage should be encouraged for better control of parthenium and higher achene yield of sunflower crop(3293.3 kg ha^(-1) in 2017 and 3221.3 kg ha^(-1) in 2018).Moreover,is also inferred that total dose of herbicide might be reduced by using hoeing and mulching in an integrated way. 展开更多
关键词 parthenium MULCH HOEING TILLAGE S-METOLACHLOR SUNFLOWER
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Impact of <i>Parthenium hysterophorus</i>L. Invasion on Species Diversity of Cultivated Fields of Bilaspur (C.G.) India
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作者 Preeti Kumari Pankaj K. Sahu +1 位作者 Madhu Y. Soni Poonam Awasthi 《Agricultural Sciences》 2014年第8期754-764,共11页
In present study invasion of Parthenium hysterophorus L. and its impacts on agriculture fields will be done in different cultivated crops in Bilaspur. The present work deals with diversity and distribution of weeds sp... In present study invasion of Parthenium hysterophorus L. and its impacts on agriculture fields will be done in different cultivated crops in Bilaspur. The present work deals with diversity and distribution of weeds species in cultivated fields of Bilaspur C.G. A total number of 28 species under 27 genera and 16 families was distributed in three study sites (Koni, Sakri & Kota) of cultivated fields in Bilaspur C.G. These study sites were located in different altitudinal zones responding different conditions in the this study. The number of species per sites ranged from 13 to 24. In the Sakri, number of species was ranged from 17 to 27, and the relative density of plant species ranged between 0.77 (Heteropogon contortus, Solanum surratense, Vernonia cinneria and Leucas aspera) and 22.92 (Parthenium hysterophorus). In the Kota, number of species was ranged from 14 to 28, and the relative density of plant species ranged between 0.85 (Ageratum conyzoides, Heteropogon contortus, Triticum aestivum) and 29.06 (Zea mays) and 30.91 (Parthenium hysterophorus). In the Agriculture field, number of species was ranged from 15 to 28, and the relative density of plant species ranged between 0.58 (Leucas aspera) and 26.23 (Triticum aestivum). Present work will give the information that weeds are harmful to herbs, crops and medicinal plants. Parthenium weed can suppress and crowd out other weed species, and could form a single dominant population in the invasion area, causing serious threat to plant community biological diversity. 展开更多
关键词 parthenium INVASION Species Diversity CROPS Bilaspur
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Distribution and Abundance of <i>Parthenium hysterophorus</i>L. in Metekel Zone, Northwest Ethiopia
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作者 Jemal Tola Horo Tesfaye Gudisa +1 位作者 Emebet Worku Derese Tilahun 《American Journal of Plant Sciences》 2020年第7期1101-1110,共10页
<i><span style="font-family:Verdana;">Parthenium hysterophorus</span></i><span style="font-family:""><span style="font-family:Verdana;"> L. is a h... <i><span style="font-family:Verdana;">Parthenium hysterophorus</span></i><span style="font-family:""><span style="font-family:Verdana;"> L. is a harmful invasive weed to plant biodiversity and human health. It is native to American tropics and first introduced to Ethiopia in the 1970s. Today, it is widely distributed across the country and severely affecting the biodiversity, crop, and animal production in the country. In the Metekel Zone, there was no report on its distribution and impacts so far. Therefore, this study was aimed to assess the distribution and abundance of the plant in the zone. The distribution and abundance data of the weed were recorded at five km intervals following all accessible roads of the zone. The result reveals that </span><i><span style="font-family:Verdana;">P. hysterophorus</span></i><span style="font-family:Verdana;"> L. was less distributed in the area with a 4.95% frequency. However, it was found abundantly growing at roadsides, wastelands, around habitation, market place, and around Zeghibridge where it can rapidly spread to most economical lands like the arable and grazing lands. Moreover, it has aggressively invaded a nursery site, which enables the weed to enter agricultural fields directly. This suggests that the weed is on a fast move to agricultural lands in the zone. The regular active development activities such as agricultural investment, construction of roads, and factories are presumed to promote its spread. Therefore, a decisive and timely decision is needed to mitigate the weed when it is still sparse and small. 展开更多
关键词 parthenium hysterophorus L. Invasive Weed Weed Control Agricultural Fields Arable Lands Grazing Lands Metekel Zone
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Comparative Efficacy of Common Broad Leaf Herbicides against an Invasive Weed: <i>Parthenium</i><i>hysterophorus</i>L.
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作者 Kazam Ali Abdul Rehman +1 位作者 Kausar Khan Philip Weyl 《Agricultural Sciences》 2020年第7期617-626,共10页
<em>Parthenium hysterophorus</em> L. (parthenium weed) is an annual weed that grows rapidly in disturbed land. It is considered as one of the most hazardous weeds in Pakistan as it poses serious health pro... <em>Parthenium hysterophorus</em> L. (parthenium weed) is an annual weed that grows rapidly in disturbed land. It is considered as one of the most hazardous weeds in Pakistan as it poses serious health problems to livestock as well as severe allergenic reactions in humans. It has invaded the Punjab and Khyber Pakhtunkhwa provinces and also been spreading in other parts of the country where it poses a risk for the grazing lands, roadsides, forests, wet lands, waste lands and of all types of cropped and non-cropped areas in Pakistan. The present studies were carried out to determine the impact of four locally available broad leaf herbicides viz;Stomp 455 CS (pendimethalin), Buctril Super 60 EC (bromoxynil + MCPA), Vantage 48 SL (glyphosate) and Logran Extra 750 WG (triasulfuron + terbutryn) (@ recommended and <span style="white-space:nowrap;"><span style="white-space:nowrap;">&#189;</span></span><span style="color:#4F4F4F;font-family:" font-size:14px;white-space:normal;background-color:#ffffff;"=""></span> of recommended dose) against <em>P. hysterophorus</em> grown in pots at research field of CABI CWA, Rawalpindi. All herbicides were applied at three growth stages (rosette, bolted and flowering). The observations for the mortality of <em>P. hysterophorus</em> were made 2 and 4 weeks after spray. The glyphosate was the most effective and reported 100% mortality of <em>P. hysterophorus</em> plants at flowering stage followed by bromoxynil + MCPA (89%), pendimethalin (80%) and triasulfuron + terbutryn (61%) at recommended dose after 4 weeks of spray. All tested herbicides achieved a mortality between 38% - 86% at rosette while 54% - 96% mortality at bolted stage after 4 weeks. Initially, 2 weeks after spray at flowering stage glyphosate caused 53% wilting followed by 49% (bromoxynil + MCPA), 33% (pendimethalin) and 9% (triasulfuron + terbutryn) at their recommended doses. The results indicated that <em>P. hysterophorus</em> is the most susceptible to glyphosate and bromoxynil + MCPA, both these herbicides are very promising for the wilting and management of parthenium weed. 展开更多
关键词 parthenium hysterophorus Annual Weed HERBICIDES Mortality WILTING
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A review on valorization,management,and applications of the hazardous weed Parthenium hysterophorus
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作者 Naveen Kumar Neeraj K.Aggarwal 《Systems Microbiology and Biomanufacturing》 2024年第2期607-619,共13页
The noxious weed Parthenium hysterophorus has spread globally since departing from its native environment over two centuries ago.Its ability to thrive is attributed to adaptive features such as the lack of natural adv... The noxious weed Parthenium hysterophorus has spread globally since departing from its native environment over two centuries ago.Its ability to thrive is attributed to adaptive features such as the lack of natural adversaries,broad adaptability,resilience to drought,insensitivity to light conditions,rapid seed production,easy seed dispersal,and allelopathic traits,enabling it to flourish in various soil types and overcome climatic constraints.We aim to eliminate the P.hysterophorus infec-tion because we are aware of its harmful effects.Attempting to limit its expansion is not a feasible strategy for eradication;instead,it can be effectively handled by harnessing it for diverse purposes.This review provides a concise overview of the P.hysterophorus issue and highlights potential uses that could offer innovative approaches to address the problem.Newly identified applications of P.hysterophorus encompass composting,serving as a bioremediation agent for hazardous metals and dyes,acting as a cost-effective substrate for cellulase production,contributing to nanoparticle synthesis,facilitating ethanol production,and serving as a biogas source. 展开更多
关键词 parthenium hysterophorus Weed management BIOCONTROL Management strategies
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种间竞争对入侵植物银胶菊生长及光合特性的影响 被引量:3
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作者 冉慧超 张运春 +1 位作者 刘守江 张桥英 《西华师范大学学报(自然科学版)》 2025年第3期231-237,共7页
为了探究种间竞争对恶性入侵杂草银胶菊(Parthenium hysterophorus)生长及光合特性的影响,设置了本地物种和入侵物种的5种不同组合,即银胶菊单种、银胶菊×紫苜蓿(Medicago sativa)混种、银胶菊×野苜蓿(M.falcata)混种、银胶菊... 为了探究种间竞争对恶性入侵杂草银胶菊(Parthenium hysterophorus)生长及光合特性的影响,设置了本地物种和入侵物种的5种不同组合,即银胶菊单种、银胶菊×紫苜蓿(Medicago sativa)混种、银胶菊×野苜蓿(M.falcata)混种、银胶菊×矮蒿(Artemisia lancea)×紫苜蓿混种、银胶菊×矮蒿×野苜蓿混种,测定不同种间关系下银胶菊生长和光合指标,计算生物量分配及相对邻株效应指数,以揭示种间竞争对银胶菊入侵策略的影响。结果表明:混种处理下银胶菊根、茎、叶及总生物量的种间关系均为竞争,野苜蓿对银胶菊的竞争强度大于紫苜蓿,野苜蓿矮蒿组合对银胶菊的竞争强度大于野苜蓿,也大于紫苜蓿矮蒿组合;种间关系降低了银胶菊株高、叶面积生物量,抑制了根系生长;与银胶菊单种相比,紫苜蓿、矮蒿紫苜蓿组合增加了银胶菊的根生物量比,降低了茎生物量比和叶生物量比,野苜蓿、矮蒿野苜蓿组合增加了银胶菊的叶生物量比,降低了根生物量比和茎生物量比;混种处理下银胶菊的光合特性参数P_(n)、G_(s)和T_(r)呈下降趋势,而C_(i)呈上升趋势。综上得出:紫苜蓿和野苜蓿都能抑制银胶菊的生长,但野苜蓿的抑制强度更大,矮蒿野苜蓿组合对银胶菊的抑制强于矮蒿紫苜蓿组合,也强于野苜蓿。 展开更多
关键词 银胶菊 种间竞争 入侵植物 生物量分配 光合特性
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基于MaxEnt模型的银胶菊及其天敌银胶菊叶甲的适生区预测
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作者 陈地宝 张玉 +4 位作者 杨鸣 林蓉 刘万学 Weyl Philip 冼晓青 《中国生物防治学报》 北大核心 2025年第2期423-435,共13页
银胶菊Parthenium hysterophorus是一种全球入侵性杂草,广泛分布于热带和亚热带地区。银胶菊叶甲Zygogramma bicolorata作为银胶菊天敌昆虫,对该杂草具有较好的防治效果。预测银胶菊及其生防天敌银胶菊叶甲的适生区可以为其生物防治和... 银胶菊Parthenium hysterophorus是一种全球入侵性杂草,广泛分布于热带和亚热带地区。银胶菊叶甲Zygogramma bicolorata作为银胶菊天敌昆虫,对该杂草具有较好的防治效果。预测银胶菊及其生防天敌银胶菊叶甲的适生区可以为其生物防治和区域管理提供参考。因此,本研究基于两个物种的全球发生点和21个环境变量,利用MaxEnt模型预测了银胶菊和银胶菊叶甲在近现代气候条件下的全球适生区。研究结果表明,影响银胶菊适生区分布的主要因子为最冷季节平均温(bio11)、最湿月份降水量(bio13)、最湿季节平均温(bio8)和人类影响指数(HII);影响银胶菊叶甲适生区分布的主要因子为最暖季节平均温(bio10)、最湿月份降水量(bio13)、等温性(bio3)和人类影响指数(HII);银胶菊和银胶菊叶甲在全球的适生区面积分别为5426.79×10^(4)km^(2)和1410.59×10^(4)km^(2),适生区重叠面积为1357.66×10^(4)km^(2),主要分布在亚洲(巴基斯坦、印度、中国南部以及大部分东南亚国家),北美洲墨西哥湾沿岸(美国中低纬度地区、墨西哥东部沿海地区),非洲西部的尼日尔河流域和东南部的赞比西河流域(赞比亚、尼日利亚等),南美洲中南部(巴西东南部地区、阿根廷北部地区等)。本研究结果为在适生区重叠区域内利用银胶菊叶甲防治银胶菊提供了依据。 展开更多
关键词 银胶菊 银胶菊叶甲 适生区 MAXENT
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基于SRAP分子标记的银胶菊遗传多样性分析 被引量:3
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作者 王美皇 高翔 关亚丽 《分子植物育种》 CAS CSCD 北大核心 2020年第1期193-199,共7页
银胶菊(Parthenium hysterophor L.)是热带地区入侵植物,主要分布在路边、荒地和农田,生长繁殖迅速,危害作物产量和人畜健康,植株具有较强的化感作用抑制农作物的生长。本研究探究银胶菊的入侵特性与遗传变异之间的相关关系,为预测其入... 银胶菊(Parthenium hysterophor L.)是热带地区入侵植物,主要分布在路边、荒地和农田,生长繁殖迅速,危害作物产量和人畜健康,植株具有较强的化感作用抑制农作物的生长。本研究探究银胶菊的入侵特性与遗传变异之间的相关关系,为预测其入侵趋势提供依据。对48对引物用优化的SRAP反应体系进行筛选,最终得到17对多态性较好的引物组合,对海南、云南、广西等15个居群共计300份样品进行遗传多样性分析。结果表明,三省分布的银胶菊与中国其它地区银胶菊居群都具有较高的遗传多样性;其中63.67%的遗传多样性来自居群间,而居群内部遗传多样性并不高;居群间基因流较低,聚类分析中遗传距离与地理距离不一致。由此推测银胶菊多以人为因素扩散。 展开更多
关键词 银胶菊(parthenium hysterophor L.) 遗传多样性 SRAP 聚类分析
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银胶菊丙二烯氧化物合成酶的表达纯化及其活性分析 被引量:1
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作者 刘云华 周秋虎 +1 位作者 张灵芝 常振战 《生物技术通报》 CAS CSCD 北大核心 2012年第8期76-82,共7页
植物来源细胞色素P450的表达和结晶都十分困难,成为成功解析该类蛋白晶体结构的瓶颈。探索了使用大肠杆菌表达系统表达、纯化银胶菊丙二烯氧化物合成酶(allene oxide synthase,AOS)的方法。氨基酸序列比对分析显示,银胶菊AOS缺失N-末端... 植物来源细胞色素P450的表达和结晶都十分困难,成为成功解析该类蛋白晶体结构的瓶颈。探索了使用大肠杆菌表达系统表达、纯化银胶菊丙二烯氧化物合成酶(allene oxide synthase,AOS)的方法。氨基酸序列比对分析显示,银胶菊AOS缺失N-末端的膜锚定序列和信号肽序列,推测其在大肠杆菌中应表达为水溶性蛋白质。试验中纯化得到的银胶菊AOS是一个分子量为54.5 kD的八聚体分子,均一性好,不含去垢剂,达到毫克量级,适合下一步的晶体培养研究;还原一氧化碳差示光谱显示该酶在450 nm处有特征吸收峰,酶活性测定得到该酶的米氏常数为45.3μmol/L,显示该重组AOS具有很高的酶活性。 展开更多
关键词 银胶菊(parthenium argentatum) 丙二烯氧化物合成酶 细胞色素P450 表达纯化 酶活性分析
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外来入侵植物银胶菊水提物对三叶鬼针草和茶条木种子萌发的化感作用 被引量:21
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作者 潘玉梅 唐赛春 +3 位作者 蒲高忠 林春蕊 陈秋霞 韦春强 《广西植物》 CAS CSCD 北大核心 2008年第4期534-538,共5页
以伴生草本植物三叶鬼针草和能源木本植物茶条木为受试植物,初步研究了外来入侵植物银胶菊根、茎、花水浸提液对二者种子萌发的化感效应。根据发芽率、化感效应敏感指数、发芽速率可以看出,银胶菊不同部位的浸提液在不同浓度下对三叶鬼... 以伴生草本植物三叶鬼针草和能源木本植物茶条木为受试植物,初步研究了外来入侵植物银胶菊根、茎、花水浸提液对二者种子萌发的化感效应。根据发芽率、化感效应敏感指数、发芽速率可以看出,银胶菊不同部位的浸提液在不同浓度下对三叶鬼针草的种子萌发的影响力不同;而对茶条木种子萌发无明显的作用。为选择适宜植物,生物替代防治银胶菊提供依据。 展开更多
关键词 银胶菊 化感作用 种子萌发 三叶鬼针草 茶条木
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入侵植物银胶菊在不同生境下表型可塑性和构件生物量 被引量:23
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作者 蒲高忠 唐赛春 +2 位作者 潘玉梅 韦春强 岑艳喜 《广西植物》 CAS CSCD 北大核心 2010年第5期641-646,625,共7页
研究了入侵植物银胶菊在4种不同小生境间花果期形态特征变化和构件生物量特征。结果显示:在植株密度小但土壤肥沃的小生境中,植株各形态指标如茎长、茎直径和花序直径等都明显高于其它小生境,在生物量结构特征上则表现为总生物量和花果... 研究了入侵植物银胶菊在4种不同小生境间花果期形态特征变化和构件生物量特征。结果显示:在植株密度小但土壤肥沃的小生境中,植株各形态指标如茎长、茎直径和花序直径等都明显高于其它小生境,在生物量结构特征上则表现为总生物量和花果生物量所占比例的升高。随着植株密度的增加以及土壤肥力下降,上述各形态指标都发生了较明显的变化,生物量投资也进行了优化配置,银胶菊表现出了较高的形态可塑性。银胶菊与觅光和竞争相关的几个指标如叶和根的比例都增加,但用于生殖构件的比例却减少了。相关分析显示,银胶菊花果期各构件生物量与高度成正相关,与密度为负相关,并受环境因素的制约。表明,较高的形态可塑性和较强的生殖配置策略可能是银胶菊成功入侵我国的重要特征。 展开更多
关键词 银胶菊 生物入侵 表型可塑性 构件生物量
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外来入侵植物银胶菊在广西的分布与危害 被引量:16
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作者 唐赛春 吕仕洪 +3 位作者 何成新 李先琨 潘玉梅 蒲高忠 《广西植物》 CAS CSCD 北大核心 2008年第2期197-200,共4页
银胶菊为一种菊科植物,原产中、南美洲。尽管在广西出现的时间不长,但分布范围较广,由路旁向荒地、耕地发展,引起了很大的危害,包括入侵道路旁,阻碍交通、破坏道路环境;入侵放牧地,减少放牧地产草量;入侵耕地,引起农作物减产;且有毒,能... 银胶菊为一种菊科植物,原产中、南美洲。尽管在广西出现的时间不长,但分布范围较广,由路旁向荒地、耕地发展,引起了很大的危害,包括入侵道路旁,阻碍交通、破坏道路环境;入侵放牧地,减少放牧地产草量;入侵耕地,引起农作物减产;且有毒,能引起人的皮炎、鼻炎及哮喘,危害人类健康。在广西发展成为一种重要的外来入侵植物,已引起科学家的注意。该文介绍了银胶菊的生物学特性和在广西的入侵、扩散等情况,提出了防治措施。 展开更多
关键词 银胶菊 外来入侵植物 广西 分布 危害 防治
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几种菊科入侵植物和非入侵植物的化感作用比较 被引量:31
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作者 李富荣 黄莹 +1 位作者 梁士楚 周巧劲 《生态环境学报》 CSCD 北大核心 2011年第5期813-818,共6页
以萝卜为受体,采用培养皿法比较研究了几种菊科入侵种和非入侵种的化感潜力。结果表明:入侵种和非入侵种都具有化感作用,且入侵种在高浓度下的化感抑制作用强度不一定都大于非入侵种。在0.2 g.mL-1浓度时,艾蒿和黄鹌菜的综合化感作用强... 以萝卜为受体,采用培养皿法比较研究了几种菊科入侵种和非入侵种的化感潜力。结果表明:入侵种和非入侵种都具有化感作用,且入侵种在高浓度下的化感抑制作用强度不一定都大于非入侵种。在0.2 g.mL-1浓度时,艾蒿和黄鹌菜的综合化感作用强度分别是小飞蓬的2.5和3.1倍;而紫茎泽兰和银胶菊则是小飞蓬的3.8和3.5倍。而不同植物浸提液对萝卜的不同检测指标影响不同。其中,紫茎泽兰对萝卜种子发芽和幼苗生长的影响主要表现为化感抑制作用。银胶菊和除0.025 g.mL-1外的黄鹌菜各浸提液都使萝卜种子萌发受较强的抑制,且抑制强度随浸提液浓度的升高而增大,而对幼苗生长则表现为"低促高抑"的规律。小飞蓬和苦荬菜对萝卜种子萌发和幼苗根长为抑制作用,但对其苗高和鲜质量为促进作用。除0.025 g.mL-1外,艾蒿对萝卜种子萌发和幼苗根长有较强的抵制,对其苗高和鲜质量则呈"低促高抑"的规律。 展开更多
关键词 生物入侵 化感机制 紫茎泽兰 银胶菊 小飞蓬
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外来入侵植物银胶菊不同部位的化感作用 被引量:9
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作者 高兴祥 李美 +5 位作者 谢慧 高宗军 张秀荣 张悦丽 曹坳程 孔金花 《草业科学》 CAS CSCD 北大核心 2012年第6期898-903,共6页
在室内以滤纸为载体用离体生物测定方法测定了银胶菊(Parthenium hysterophorus)根、茎、叶和果实水浸提液对蔬菜萝卜(Raphanus sativus)和杂草马唐(Digitaria sanguinalis)、苘麻(Abutilon theophrasti)种子的化感效应,同时在温室内以... 在室内以滤纸为载体用离体生物测定方法测定了银胶菊(Parthenium hysterophorus)根、茎、叶和果实水浸提液对蔬菜萝卜(Raphanus sativus)和杂草马唐(Digitaria sanguinalis)、苘麻(Abutilon theophrasti)种子的化感效应,同时在温室内以土壤为载体测定了银胶菊不同部位干粉对这3种植物种子萌发和幼苗生长的影响。室内生测试验结果表明,银胶菊叶和果实水浸提物对3种受体植物种子的萌发和幼苗生长均有较强的抑制作用,其中受体萝卜和苘麻的敏感性较高,而马唐的敏感性较低。温室盆栽试验也表明,银胶菊叶和果实对受体生长的影响明显高于根和茎,说明叶和果实是集中表现银胶菊化感作用的特定部位。 展开更多
关键词 银胶菊 化感作用 叶片 果实
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银胶菊叶和花提取物对南方根结线虫的毒杀活性比较 被引量:9
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作者 苏秀荣 谢宁 +1 位作者 张纪龙 王金信 《植物资源与环境学报》 CAS CSCD 北大核心 2012年第1期77-82,共6页
为进一步明确银胶菊(Parthenium hysterophorus L.)的杀线虫活性,对银胶菊叶和花的不同溶剂提取物、甲醇提取物的不同萃取物以及甲醇提取物碱水层的不同极性组分对南方根结线虫(Meloidogyne incognita Chitwood)的杀虫活性进行了检测,... 为进一步明确银胶菊(Parthenium hysterophorus L.)的杀线虫活性,对银胶菊叶和花的不同溶剂提取物、甲醇提取物的不同萃取物以及甲醇提取物碱水层的不同极性组分对南方根结线虫(Meloidogyne incognita Chitwood)的杀虫活性进行了检测,并对不同提取物、萃取物和萃取组分进行了生物碱的定性分析。结果表明:银胶菊叶和花的蒸馏水、甲醇、乙酸乙酯和石油醚提取物的得率分别为24.5%和20.3%、19.6%和10.9%、6.8%和7.7%、2.0%和2.7%,其中,叶和花的蒸馏水和甲醇提取物的杀线虫活性均较强,而石油醚提取物的杀线虫活性最弱。用质量体积分数1.0%和0.5%的叶和花蒸馏水提取物分别处理24和48 h后试虫的校正死亡率均达到100.00%;用质量体积分数1.0%和0.5%的叶和花甲醇提取物处理48 h,试虫的校正死亡率均大于90%。叶和花甲醇提取物的碱水层、三氯甲烷Ⅰ层和Ⅱ层萃取物均具有一定的杀线虫活性,其中,用质量体积分数1.0%的花和叶碱水层萃取物以及花的三氯甲烷Ⅰ层萃取物分别处理48 h,试虫的校正死亡率均为100.00%,而三氯甲烷Ⅱ层萃取物的杀线虫活性最弱。银胶菊叶和花甲醇提取物碱水层的11个不同极性组分(A1~A11)也表现出不同程度的杀线虫活性,其中,用质量体积分数0.2%和0.1%花的A2〔溶剂为V(三氯甲烷)∶V(甲醇)=10∶1〕和A7〔溶剂为V(三氯甲烷)∶V(甲醇)=1∶1〕组分以及叶的A2和A6〔溶剂为V(三氯甲烷)∶V(甲醇)=2∶1〕组分处理48 h后,试虫的校正死亡率均达100.00%,显著高于其他组分。定性实验结果表明:银胶菊叶和花中具有杀线虫活性的提取物、萃取物和萃取组分中均含有生物碱。研究结果说明:银胶菊花的杀线虫活性高于叶片,其毒杀活性不仅与提取部位及溶剂的种类和极性有关,还与提取物浓度及作用时间等因素有关。 展开更多
关键词 银胶菊 提取物 南方根结线虫 校正死亡率 生物碱
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