Objective: To examine the effect of Pandanus amaryllifolius(P. amaryllifolius) leaf extract on the insulin resistance state in obese ICR mice.Methods: Obesity was induced in mice fed with high-fat diet(45% fat) for 12...Objective: To examine the effect of Pandanus amaryllifolius(P. amaryllifolius) leaf extract on the insulin resistance state in obese ICR mice.Methods: Obesity was induced in mice fed with high-fat diet(45% fat) for 12 weeks. After the first six weeks on the diet, the obese mice were administered with the water extract of P. amaryllifolius leaf at 125 and 250 mg/kg/day, respectively for another six weeks. At the5 th week of treatment, oral glucose tolerance test was conducted. After six weeks of treatment, the levels of blood glucose, serum insulin, leptin, adiponectin, and lipid profiles were determined. The liver, muscle and epididymal fat tissues were removed for measuring the biochemical parameters and protein expression, as well as histological examination.Results: Six weeks of treatment with P. amaryllifolius led to a significant reduction in the blood glucose level as well as improvement in the insulin resistance. P. amaryllifolius also increased the liver glycogen storage and serum adiponectin and decreased the serum leptin levels. A reduction in the serum and hepatic triglyceride, and non-esterified fatty acid levels was also observed. The histological examination showed that the obese mice treated with P. amaryllifolius reduced the lipid droplet in liver tissue and adipocyte size in epididymal fat tissue. The treatment also increased the protein expression of glucose transporter 4 in the muscle and fat tissues.Conclusions: The treatment with P. amaryllifolius could decrease several parameters of impaired glucose and lipid metabolism. To the best of our knowledge, this is the first report on the role of P. amaryllifolius leaf extract in alleviating the insulin dysfunction in obesity state.展开更多
Effective control of eutrophication is generally established through the reduction of nutrient loading into waterways and water bodies. An economically viable and ecologically sustainable approach to nutrient pollutio...Effective control of eutrophication is generally established through the reduction of nutrient loading into waterways and water bodies. An economically viable and ecologically sustainable approach to nutrient pollution control could involve the integration of retention ponds, wetlands and greenways into water management systems. Plants not only play an invaluable role in the assimilation and removal of nutrients, but they also support fauna richness and can be aesthetically pleasing. Pandanus amaryllifolius, a tropical terrestrial plant, was found to establish well in hydrophytic conditions and was highly effective in remediating high nutrient levels in an aquatic environment showing 100% removal of NO^-N up to 200 mg/L in 14 days. Phosphate uptake by the plant was less efficient with 64% of the PO4-P removed at the maximum concentration of 100 mg/L at the end of 6 weeks. With its high NO^-N and PO43--P removal efficiency, P. amaryllifolius depleted the nutrient-rich media and markedly contained the natural colonization of algae. The impediment of algal growth led to improvements in the water quality with significant decreases in turbidity, pH and electrical conductivity. In addition, the plants did not show stress symptoms when grown in high nutrient levels as shown by the changes in their biomass, total soluble proteins and chlorophyll accumulation as well as photochemical efficiency. Thus, P. amaryUifolius is a potential candidate for the mitigation of nutrient pollution in phytoremediation systems in the tropics as the plant requires low maintenance, is tolerant to the natural variability of weather conditions and fluctuating hydro-periods, and exhibit good nutrient removal capabilities.展开更多
Objective:To evaluate the anti-inflammatory and analgesic activities of the ethanol and aqueous extracts of prop roots of Pandanus fascicularis(P.fascicularis) Lam(pandanaceae).And provide experimental evidence for it...Objective:To evaluate the anti-inflammatory and analgesic activities of the ethanol and aqueous extracts of prop roots of Pandanus fascicularis(P.fascicularis) Lam(pandanaceae).And provide experimental evidence for its traditional use such as rheumatoid arthritis and spasmodic. Methods:The anti-inflammatory activity was observed by carrageenan-induced edema of the hind paw of rats.Analgesic activities of prop roots of P.fascicularis were determined using acetic acid induced writhing model and tail clip method in mice and rat,respectively.The ethanol fraction was then subjected to chromatographic analysis and a compound has been isolated and characterized by IR,~1H-NMR and mass spectroscopy.Results:Edema suppressant effect of ethanol extract was found to be 37.03%inhibition whereas aqueous extract was found to be 63.22%inhibition after 3 h which was nearly equivalent to that of 10 mg/kg of indomethacin (67.81%).Percentage inhibition of writhing compared to control were 63.15%,54.38%,14.90%for aspirin,aqueous extract and ethanolic extract,respectively.Both ethanol and aqueous extracts show significant activity against appropriate controls after 60 min of treatment on tail clip method. The structure of the isolated compound is may be characterized as Hepta deca-5-ene-l-ol by analysis it’s IR,H-NMR and mass spectroscopy data.Conclusions:The extracts of prop roots of P.fascicularis produce significant analgesic and anti-inflammatory activities,supporting the traditional application of this herb in treating various diseases associated with inflammation and pain.展开更多
OBJECTIVE To investigate the effect of Pandanus amaryllifolius leaf in high-fat diet-induced insulin resistance in mice model.METHODS To induce obesity,male ICR mice were fed with a high-fat diet(45%fat)for six weeks....OBJECTIVE To investigate the effect of Pandanus amaryllifolius leaf in high-fat diet-induced insulin resistance in mice model.METHODS To induce obesity,male ICR mice were fed with a high-fat diet(45%fat)for six weeks.The mice were divided into four groups(n=8):non-obese control mice were treated with 5% gum arabic and obese mice were treated with Pandanus amaryllifolius(125and 250mg·kg-1·d-1),or 5% gum arabic.After six weeks of treatments,the fasting blood glucose,serum insulin,OGTT and fat cell protein expression of glucose transporter 4(GLUT4)were determined.RESULTS Administration of Pandanus amaryllifolius showed significantly(P<0.05)reduced the high blood glucose,inhibited the abnormal increase in blood glucose level during OGTT,and decreased the high level of serum insulin.Moreover,it is interesting that the protein expression of GLUT4 was effectively increased by Pandanus amaryllifolius.CONCLUSION These findings demonstrate that the extract from Pandanus amaryllifolius leaf possesses antihyperglycemic action in obese mice by improving insulin sensitivity and stimulating GLUT4 expression in adipose tissue.展开更多
[Objectives] This study was conducted to establish the quality control standards for Pandanus tectorius Soland.[Methods] Microscopic identification and thin layer chromatography were used to identify and determine the...[Objectives] This study was conducted to establish the quality control standards for Pandanus tectorius Soland.[Methods] Microscopic identification and thin layer chromatography were used to identify and determine the moisture,total ash and extract contents.[Results] The root transection is near round.The outmost side is velamen.There are nearly 10 columns of collenchyma tissue cells inside.In the cortex,there are a large number of fiber bundles scattered,and there are also large mucous cells scattered.The endodermis is distinct,and the cells are small and closely arranged.The pericycle is distinct and forms a ring.The pericycle fiber bundles are arranged in a radial shape by more than 40 bundles of phloem and xylem alternately.The leaf transection is V-shaped.The collenchyma lies under the epidermis.The spongy tissue has many calcium oxalate square crystal and needle crystals.The mucous cells contain calcium oxalate square crystal and needle crystals.The thin layer identification indicated that in the chromatogram of each test sample,spots of the same colors appeared at the positions corresponding to the chromatogram of the reference medicinal material,and the reproducibility was good.The moisture contents of the 10 batches of samples were all below 8.0%.The total ash was below 8.0%.The acid-insoluble ash was below 2.0%.With 50% ethanol as the solvent,the extract contents were all over 12.5%.[Conclusions] The microscopic identification results are reliable and can be used as the identification basis of P.tectorius.The thin layer identification method and the test results can provide a basis for quality control of P.tectorius.展开更多
Microbes are good sources of biologically active compounds that can be used as pharmaceuticals and agrochemicals. As part of our continuous efforts in search for biopesticides from natural sources, a fungus is isolate...Microbes are good sources of biologically active compounds that can be used as pharmaceuticals and agrochemicals. As part of our continuous efforts in search for biopesticides from natural sources, a fungus is isolated from leaves of Pandanus amaryllifolius that shows severe necrosis. This fungus is cultured in potato dextrose agar and identified as Curvularia intermedia. The ethyl acetate extract of the liquid culture medium is phytotoxic. Identification and bioassay of αβ-dehydrocurvularin (1) and curvularin (2) from this fungus are described. Monoacetyldehydrocurvularin (3), and diacetyldehydrocurvularin (4) are synthesized from 1. Phytoxicity rankings in a variety of bioassays are 1 > 2 > 4 > 3.展开更多
This paper provides illustrated descriptions of micro-fungi newly found on Pandanaceae in China and Thailand.The fungi are accommodated in 31 families.New taxa described include a new family,seven new genera,65 new sp...This paper provides illustrated descriptions of micro-fungi newly found on Pandanaceae in China and Thailand.The fungi are accommodated in 31 families.New taxa described include a new family,seven new genera,65 new species,16 previously known species.A new family:Malaysiascaceae(Glomerellales).New genera are Acremoniisimulans(Plec-tosphaerellaceae),Pandanaceomyces,Pseudoachroiostachy(Nectriaceae),Pseudohyaloseta(Niessliaceae),Pseudoor-natispora(Stachybotriaceae)and Yunnanomyces(Sympoventuriaceae).New species are Acremoniisimulans thailandensis,Beltrania krabiensis,Beltraniella pandanicola,B.thailandicus,Canalisporium krabiense,C.thailandensis,Clonostachys krabiensis,Curvularia chonburiensis,C.pandanicola,C.thailandicum,C.xishuangbannaensis,Cylindrocladiella xishuangbannaensis,Dictyochaeta pandanicola,Dictyocheirospora nabanheensis,D.pandanicola,D.xishuangbannaen-sis,Dictyosporium appendiculatum,Di.guttulatum,Di.hongkongensis,Di.krabiense,Di.pandanicola,Distoseptispora thailandica,D.xishuangbannaensis,Helicoma freycinetiae,Hermatomyces biconisporus,Lasiodiplodia chonburiensis,L.pandanicola,Lasionectria krabiense,Menisporopsis pandanicola,Montagnula krabiensis,Musicillium pandanicola,Neofusicoccum pandanicola,Neohelicomyces pandanicola,Neooccultibambusa thailandensis,Neopestalotiopsis chiang-maiensis,N.pandanicola,N.phangngaensis,Pandanaceomyces krabiensis,Paracylindrocarpon nabanheensis,P.pan-danicola,P.xishuangbannaensis,Parasarcopodium hongkongensis,Pestalotiopsis krabiensis,P.pandanicola,Polyplosphaeria nabanheensis,P.pandanicola,P.xishuangbannaensis,Pseudoachroiostachys krabiense,Pseu-doberkleasmium pandanicola,Pseudochaetosphaeronema pandanicola,Pseudohyaloseta pandanicola,Pseudoornatispora krabiense,Pseudopithomyces pandanicola,Rostriconidium pandanicola,Sirastachys phangngaensis,Stictis pandanicola,Terriera pandanicola,Thozetella pandanicola,Tubeufia freycinetiae,T.parvispora,T.pandanicola,Vermiculariopsiella hongkongensis,Volutella krabiense,V.thailandensis and Yunnanomyces pandanicola.Previous studies of micro-fungi on Pandanaceae have not included phylogenetic support.Inspiration for this study came from the book Fungi Associated with Pandanaceae by Whitton,McKenzie and Hyde in 2012.Both studies reveal that the micro-fungi on Pandanaceae is particularly rich in hyphomycetes.All data presented herein are based on morphological examination of specimens,coupled with phylogenetic sequence data to better integrate taxa into appropriate taxonomic ranks and infer their evolu-tionary relationships.展开更多
基金Supported by the research grant from the Faculty of Medicine,Thammasat University(Contract number:GEN2-22/2015)
文摘Objective: To examine the effect of Pandanus amaryllifolius(P. amaryllifolius) leaf extract on the insulin resistance state in obese ICR mice.Methods: Obesity was induced in mice fed with high-fat diet(45% fat) for 12 weeks. After the first six weeks on the diet, the obese mice were administered with the water extract of P. amaryllifolius leaf at 125 and 250 mg/kg/day, respectively for another six weeks. At the5 th week of treatment, oral glucose tolerance test was conducted. After six weeks of treatment, the levels of blood glucose, serum insulin, leptin, adiponectin, and lipid profiles were determined. The liver, muscle and epididymal fat tissues were removed for measuring the biochemical parameters and protein expression, as well as histological examination.Results: Six weeks of treatment with P. amaryllifolius led to a significant reduction in the blood glucose level as well as improvement in the insulin resistance. P. amaryllifolius also increased the liver glycogen storage and serum adiponectin and decreased the serum leptin levels. A reduction in the serum and hepatic triglyceride, and non-esterified fatty acid levels was also observed. The histological examination showed that the obese mice treated with P. amaryllifolius reduced the lipid droplet in liver tissue and adipocyte size in epididymal fat tissue. The treatment also increased the protein expression of glucose transporter 4 in the muscle and fat tissues.Conclusions: The treatment with P. amaryllifolius could decrease several parameters of impaired glucose and lipid metabolism. To the best of our knowledge, this is the first report on the role of P. amaryllifolius leaf extract in alleviating the insulin dysfunction in obesity state.
基金the support & contributions of the Singapore-Delft Water Alliance (SDWA)
文摘Effective control of eutrophication is generally established through the reduction of nutrient loading into waterways and water bodies. An economically viable and ecologically sustainable approach to nutrient pollution control could involve the integration of retention ponds, wetlands and greenways into water management systems. Plants not only play an invaluable role in the assimilation and removal of nutrients, but they also support fauna richness and can be aesthetically pleasing. Pandanus amaryllifolius, a tropical terrestrial plant, was found to establish well in hydrophytic conditions and was highly effective in remediating high nutrient levels in an aquatic environment showing 100% removal of NO^-N up to 200 mg/L in 14 days. Phosphate uptake by the plant was less efficient with 64% of the PO4-P removed at the maximum concentration of 100 mg/L at the end of 6 weeks. With its high NO^-N and PO43--P removal efficiency, P. amaryllifolius depleted the nutrient-rich media and markedly contained the natural colonization of algae. The impediment of algal growth led to improvements in the water quality with significant decreases in turbidity, pH and electrical conductivity. In addition, the plants did not show stress symptoms when grown in high nutrient levels as shown by the changes in their biomass, total soluble proteins and chlorophyll accumulation as well as photochemical efficiency. Thus, P. amaryUifolius is a potential candidate for the mitigation of nutrient pollution in phytoremediation systems in the tropics as the plant requires low maintenance, is tolerant to the natural variability of weather conditions and fluctuating hydro-periods, and exhibit good nutrient removal capabilities.
文摘Objective:To evaluate the anti-inflammatory and analgesic activities of the ethanol and aqueous extracts of prop roots of Pandanus fascicularis(P.fascicularis) Lam(pandanaceae).And provide experimental evidence for its traditional use such as rheumatoid arthritis and spasmodic. Methods:The anti-inflammatory activity was observed by carrageenan-induced edema of the hind paw of rats.Analgesic activities of prop roots of P.fascicularis were determined using acetic acid induced writhing model and tail clip method in mice and rat,respectively.The ethanol fraction was then subjected to chromatographic analysis and a compound has been isolated and characterized by IR,~1H-NMR and mass spectroscopy.Results:Edema suppressant effect of ethanol extract was found to be 37.03%inhibition whereas aqueous extract was found to be 63.22%inhibition after 3 h which was nearly equivalent to that of 10 mg/kg of indomethacin (67.81%).Percentage inhibition of writhing compared to control were 63.15%,54.38%,14.90%for aspirin,aqueous extract and ethanolic extract,respectively.Both ethanol and aqueous extracts show significant activity against appropriate controls after 60 min of treatment on tail clip method. The structure of the isolated compound is may be characterized as Hepta deca-5-ene-l-ol by analysis it’s IR,H-NMR and mass spectroscopy data.Conclusions:The extracts of prop roots of P.fascicularis produce significant analgesic and anti-inflammatory activities,supporting the traditional application of this herb in treating various diseases associated with inflammation and pain.
基金The project supported by Faculty of Medicine,Thammasat University Thailand
文摘OBJECTIVE To investigate the effect of Pandanus amaryllifolius leaf in high-fat diet-induced insulin resistance in mice model.METHODS To induce obesity,male ICR mice were fed with a high-fat diet(45%fat)for six weeks.The mice were divided into four groups(n=8):non-obese control mice were treated with 5% gum arabic and obese mice were treated with Pandanus amaryllifolius(125and 250mg·kg-1·d-1),or 5% gum arabic.After six weeks of treatments,the fasting blood glucose,serum insulin,OGTT and fat cell protein expression of glucose transporter 4(GLUT4)were determined.RESULTS Administration of Pandanus amaryllifolius showed significantly(P<0.05)reduced the high blood glucose,inhibited the abnormal increase in blood glucose level during OGTT,and decreased the high level of serum insulin.Moreover,it is interesting that the protein expression of GLUT4 was effectively increased by Pandanus amaryllifolius.CONCLUSION These findings demonstrate that the extract from Pandanus amaryllifolius leaf possesses antihyperglycemic action in obese mice by improving insulin sensitivity and stimulating GLUT4 expression in adipose tissue.
基金Supported by Study on Quality Evaluation and Standards of Guangxi Zhuang Medicine(MZY2013017)Guangxi Key Laboratory of Zhuang Yao Medicine(GKJZ[2014]32)Zhuang Yao Medicine Collaborative Innovation Center(GJKY[2013]20)
文摘[Objectives] This study was conducted to establish the quality control standards for Pandanus tectorius Soland.[Methods] Microscopic identification and thin layer chromatography were used to identify and determine the moisture,total ash and extract contents.[Results] The root transection is near round.The outmost side is velamen.There are nearly 10 columns of collenchyma tissue cells inside.In the cortex,there are a large number of fiber bundles scattered,and there are also large mucous cells scattered.The endodermis is distinct,and the cells are small and closely arranged.The pericycle is distinct and forms a ring.The pericycle fiber bundles are arranged in a radial shape by more than 40 bundles of phloem and xylem alternately.The leaf transection is V-shaped.The collenchyma lies under the epidermis.The spongy tissue has many calcium oxalate square crystal and needle crystals.The mucous cells contain calcium oxalate square crystal and needle crystals.The thin layer identification indicated that in the chromatogram of each test sample,spots of the same colors appeared at the positions corresponding to the chromatogram of the reference medicinal material,and the reproducibility was good.The moisture contents of the 10 batches of samples were all below 8.0%.The total ash was below 8.0%.The acid-insoluble ash was below 2.0%.With 50% ethanol as the solvent,the extract contents were all over 12.5%.[Conclusions] The microscopic identification results are reliable and can be used as the identification basis of P.tectorius.The thin layer identification method and the test results can provide a basis for quality control of P.tectorius.
文摘Microbes are good sources of biologically active compounds that can be used as pharmaceuticals and agrochemicals. As part of our continuous efforts in search for biopesticides from natural sources, a fungus is isolated from leaves of Pandanus amaryllifolius that shows severe necrosis. This fungus is cultured in potato dextrose agar and identified as Curvularia intermedia. The ethyl acetate extract of the liquid culture medium is phytotoxic. Identification and bioassay of αβ-dehydrocurvularin (1) and curvularin (2) from this fungus are described. Monoacetyldehydrocurvularin (3), and diacetyldehydrocurvularin (4) are synthesized from 1. Phytoxicity rankings in a variety of bioassays are 1 > 2 > 4 > 3.
文摘This paper provides illustrated descriptions of micro-fungi newly found on Pandanaceae in China and Thailand.The fungi are accommodated in 31 families.New taxa described include a new family,seven new genera,65 new species,16 previously known species.A new family:Malaysiascaceae(Glomerellales).New genera are Acremoniisimulans(Plec-tosphaerellaceae),Pandanaceomyces,Pseudoachroiostachy(Nectriaceae),Pseudohyaloseta(Niessliaceae),Pseudoor-natispora(Stachybotriaceae)and Yunnanomyces(Sympoventuriaceae).New species are Acremoniisimulans thailandensis,Beltrania krabiensis,Beltraniella pandanicola,B.thailandicus,Canalisporium krabiense,C.thailandensis,Clonostachys krabiensis,Curvularia chonburiensis,C.pandanicola,C.thailandicum,C.xishuangbannaensis,Cylindrocladiella xishuangbannaensis,Dictyochaeta pandanicola,Dictyocheirospora nabanheensis,D.pandanicola,D.xishuangbannaen-sis,Dictyosporium appendiculatum,Di.guttulatum,Di.hongkongensis,Di.krabiense,Di.pandanicola,Distoseptispora thailandica,D.xishuangbannaensis,Helicoma freycinetiae,Hermatomyces biconisporus,Lasiodiplodia chonburiensis,L.pandanicola,Lasionectria krabiense,Menisporopsis pandanicola,Montagnula krabiensis,Musicillium pandanicola,Neofusicoccum pandanicola,Neohelicomyces pandanicola,Neooccultibambusa thailandensis,Neopestalotiopsis chiang-maiensis,N.pandanicola,N.phangngaensis,Pandanaceomyces krabiensis,Paracylindrocarpon nabanheensis,P.pan-danicola,P.xishuangbannaensis,Parasarcopodium hongkongensis,Pestalotiopsis krabiensis,P.pandanicola,Polyplosphaeria nabanheensis,P.pandanicola,P.xishuangbannaensis,Pseudoachroiostachys krabiense,Pseu-doberkleasmium pandanicola,Pseudochaetosphaeronema pandanicola,Pseudohyaloseta pandanicola,Pseudoornatispora krabiense,Pseudopithomyces pandanicola,Rostriconidium pandanicola,Sirastachys phangngaensis,Stictis pandanicola,Terriera pandanicola,Thozetella pandanicola,Tubeufia freycinetiae,T.parvispora,T.pandanicola,Vermiculariopsiella hongkongensis,Volutella krabiense,V.thailandensis and Yunnanomyces pandanicola.Previous studies of micro-fungi on Pandanaceae have not included phylogenetic support.Inspiration for this study came from the book Fungi Associated with Pandanaceae by Whitton,McKenzie and Hyde in 2012.Both studies reveal that the micro-fungi on Pandanaceae is particularly rich in hyphomycetes.All data presented herein are based on morphological examination of specimens,coupled with phylogenetic sequence data to better integrate taxa into appropriate taxonomic ranks and infer their evolu-tionary relationships.
