To address critical challenges of protein template denaturation caused by intense exothermicity and prolonged reaction time when using the traditional protein molecular imprinting technology,a novel imprinting strateg...To address critical challenges of protein template denaturation caused by intense exothermicity and prolonged reaction time when using the traditional protein molecular imprinting technology,a novel imprinting strategy was proposed.This study successfully achieved the rapid and controllable in-situ synthesis of polyacrylamide/calcium alginate(PAM/CaAlg)hydrogel films under near-ambient temperature conditions,employing a silver ions(Ag^(+))-catalyzed ammonium persulfate-sodium bisulfite redox system with acrylamide(AM)as the monomer,N,N′-methylenebisacrylamide(MBA)as the crosslinker,and bovine serum albumin(BSA)as the template.The optimized molecularly imprinted polymer(MIP)films demonstrated substantial enhancement of the BSA adsorption capacity following the removal of templates,reaching a maximum equilibrium adsorption capacity(Qe)of 50.4 mg·g-1 while maintaining a stable imprinting efficiency(IE)of 2.7.Competitive adsorption experiments verified the exceptional selectivity of MIP films towards the BSA recognition.Additionally,the incorporation of Ag^(+)ions endowed both MIP and nonimprinted polymer(NIP)films with remarkable antibacterial properties.This work establishes a straightforward and effective methodology for developing advanced protein-imprinted hydrogels that simultaneously exhibit high adsorption capacity,superior selectivity,and significant antibacterial activity.展开更多
基金supported by the project of open bidding for selecting the best candidates in Tianjin Institute of Acupuncture and Moxibustion,and the National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion(ZJJBGS2024002-2).
文摘To address critical challenges of protein template denaturation caused by intense exothermicity and prolonged reaction time when using the traditional protein molecular imprinting technology,a novel imprinting strategy was proposed.This study successfully achieved the rapid and controllable in-situ synthesis of polyacrylamide/calcium alginate(PAM/CaAlg)hydrogel films under near-ambient temperature conditions,employing a silver ions(Ag^(+))-catalyzed ammonium persulfate-sodium bisulfite redox system with acrylamide(AM)as the monomer,N,N′-methylenebisacrylamide(MBA)as the crosslinker,and bovine serum albumin(BSA)as the template.The optimized molecularly imprinted polymer(MIP)films demonstrated substantial enhancement of the BSA adsorption capacity following the removal of templates,reaching a maximum equilibrium adsorption capacity(Qe)of 50.4 mg·g-1 while maintaining a stable imprinting efficiency(IE)of 2.7.Competitive adsorption experiments verified the exceptional selectivity of MIP films towards the BSA recognition.Additionally,the incorporation of Ag^(+)ions endowed both MIP and nonimprinted polymer(NIP)films with remarkable antibacterial properties.This work establishes a straightforward and effective methodology for developing advanced protein-imprinted hydrogels that simultaneously exhibit high adsorption capacity,superior selectivity,and significant antibacterial activity.
