时间序列聚类中的相似度度量方法选择已成为研究热点。目前大多数的聚类方法使用欧式距离进行相似性度量,但欧式距离进行度量对结构复杂的时间序列适用性较差,不能很好的提高聚类的准确性。提出一种基于动态时间弯曲(Dynamic Time Warpi...时间序列聚类中的相似度度量方法选择已成为研究热点。目前大多数的聚类方法使用欧式距离进行相似性度量,但欧式距离进行度量对结构复杂的时间序列适用性较差,不能很好的提高聚类的准确性。提出一种基于动态时间弯曲(Dynamic Time Warping)的PAM(Partitioning Around Medoids)算法,该方法在PAM算法的基础上引入DTW,以发现时间序列中的相似模式,增强对时间序列的偏移、振幅变化等情况的鲁棒性。在UCR数据集上的实验结果验证了PAM-DTW的准确率和稳定性优于传统算法。展开更多
CRISPR-Cas technology has revolutionized our ability to understand and engineer organisms,evolving from a singular Cas9 model to a diverse CRISPR toolbox.A critical bottleneck in developing new Cas proteins is identif...CRISPR-Cas technology has revolutionized our ability to understand and engineer organisms,evolving from a singular Cas9 model to a diverse CRISPR toolbox.A critical bottleneck in developing new Cas proteins is identifying protospacer adjacent motif(PAM)sequences.Due to the limitations of experimental methods,bioinformatics approaches have become essential.However,existing PAM prediction programs are limited by the small number of spacers in CRISPR-Cas systems,resulting in low accuracy.To address this,we develop PAMPHLET,a pipeline that uses homology searches to identify additional spacers,significantly increasing the number of spacers up to 18-fold.PAMPHLET is validated on 20 CRISPR-Cas systems and successfully predicts PAM sequences for 18 protospacers.These predictions are further validated using the DocMF platform,which characterizes protein-DNA recognition patterns via next-generation sequencing.The high consistency between PAMPHLET predictions and DocMF results for Cas proteins demonstrates the potential of PAMPHLET to enhance PAM sequence prediction accuracy,expedite the discovery process,and accelerate the development of CRISPR tools.展开更多
The adsorption of phosphate was conducted by the complex of chitosan/polyacrylamide/ferric(CS/PAM/Fe(Ⅲ))prepared.The SEM images and XPS spectra confirmed the successful adsorption of phosphate.The adsorption process ...The adsorption of phosphate was conducted by the complex of chitosan/polyacrylamide/ferric(CS/PAM/Fe(Ⅲ))prepared.The SEM images and XPS spectra confirmed the successful adsorption of phosphate.The adsorption process was studied by varying the influencing aspects like pH,co-existing ions,contact time,and initial phosphate concentration.The experimental results indicate that the adsorptive capacity decreases with the increase of pH.However,it is commendable that there is still a adsorption capacity of more than 5 mg/g when the pH is 8-11.The adsorption kinetics can be accurately described by the pseudo-second-order model and is controlled by both chemisorption and surface diffusion.The adsorption process is a single layer adsorption.This paper proposed that the adsorption mechanism of CS/PAM/Fe(Ⅲ)complex is the joint action of electrostatic attraction and ligand exchange.展开更多
文摘时间序列聚类中的相似度度量方法选择已成为研究热点。目前大多数的聚类方法使用欧式距离进行相似性度量,但欧式距离进行度量对结构复杂的时间序列适用性较差,不能很好的提高聚类的准确性。提出一种基于动态时间弯曲(Dynamic Time Warping)的PAM(Partitioning Around Medoids)算法,该方法在PAM算法的基础上引入DTW,以发现时间序列中的相似模式,增强对时间序列的偏移、振幅变化等情况的鲁棒性。在UCR数据集上的实验结果验证了PAM-DTW的准确率和稳定性优于传统算法。
基金supported by grants from the Foundation for Distinguished Young Talents in Higher Education of Guangdong,China(2024KQNCX157)Our work was also supported in part by the Guangdong Provincial Key Laboratory of Interdisciplinary Research and Application for Data Science,BNU-HKBU United International College(2022B1212010006)+1 种基金in part by the Guangdong Higher Education Upgrading Plan(2021-2025)of“Rushing to the Top,Making Up Shortcomings and Strengthening Special Features”(R0400001-22)Additionally,we acknowledge support from the Zhuhai Basic and Applied Basic ResearchFoundation(2220004002717).
文摘CRISPR-Cas technology has revolutionized our ability to understand and engineer organisms,evolving from a singular Cas9 model to a diverse CRISPR toolbox.A critical bottleneck in developing new Cas proteins is identifying protospacer adjacent motif(PAM)sequences.Due to the limitations of experimental methods,bioinformatics approaches have become essential.However,existing PAM prediction programs are limited by the small number of spacers in CRISPR-Cas systems,resulting in low accuracy.To address this,we develop PAMPHLET,a pipeline that uses homology searches to identify additional spacers,significantly increasing the number of spacers up to 18-fold.PAMPHLET is validated on 20 CRISPR-Cas systems and successfully predicts PAM sequences for 18 protospacers.These predictions are further validated using the DocMF platform,which characterizes protein-DNA recognition patterns via next-generation sequencing.The high consistency between PAMPHLET predictions and DocMF results for Cas proteins demonstrates the potential of PAMPHLET to enhance PAM sequence prediction accuracy,expedite the discovery process,and accelerate the development of CRISPR tools.
基金Funded by the Provincial Natural Science Foundation for Universities of AnhuiChina(No.KJ2021A0624)+1 种基金the Director's Fund of Anhui Province Advanced Building Materials International Research Center(No.JZCL2207ZR)the Anhui Jianzhu University Talent Introduction and Doctoral Start-up Fund(No.2023QDZ23)。
文摘The adsorption of phosphate was conducted by the complex of chitosan/polyacrylamide/ferric(CS/PAM/Fe(Ⅲ))prepared.The SEM images and XPS spectra confirmed the successful adsorption of phosphate.The adsorption process was studied by varying the influencing aspects like pH,co-existing ions,contact time,and initial phosphate concentration.The experimental results indicate that the adsorptive capacity decreases with the increase of pH.However,it is commendable that there is still a adsorption capacity of more than 5 mg/g when the pH is 8-11.The adsorption kinetics can be accurately described by the pseudo-second-order model and is controlled by both chemisorption and surface diffusion.The adsorption process is a single layer adsorption.This paper proposed that the adsorption mechanism of CS/PAM/Fe(Ⅲ)complex is the joint action of electrostatic attraction and ligand exchange.
